Click here to switch to the map view.

The map label for this gene is nadE [H]

Identifier: 187934473

GI number: 187934473

Start: 1113959

End: 1115857

Strand: Reverse

Name: nadE [H]

Synonym: CLL_A1089

Alternate gene names: 187934473

Gene position: 1115857-1113959 (Counterclockwise)

Preceding gene: 187933879

Following gene: 187935225

Centisome position: 29.36

GC content: 29.38

Gene sequence:

>1899_bases
ATGGATTTTATAAAAGTTGCATCCGCATGTCCAAAAACTAAAGTTGGAGATATTGATTATAATATCTCTAATATTTTGAA
ATGTATAGATGATGCTAAAAATAATAATAGTAAGTTTATAGTCTTCCCAGAACTATGTATAACTTCATATACATGTGGAG
ATTTATTCTTAAATGATACTTTATTAAACAAATCAATAACTGGAATAAATCAAATATTAAACGCTACAGAAGATTGCGAT
ATGTTGATTGCACTAGGAGCACCATTACTTATAAATAACGTACTTTATAATTGCGCTTATTTACTTTTCAAAGGTAAAAT
ATTAGGAATAGTCCCTAAATCATATATACCTAACTATAGTGAATTTTATGAAAAAAGATGGTTTACAGAAGGACTTTCTT
TAGAAACACAAGAAATAGATTTACCTATTCAAAAAAATGTACCTTTTGGAGCAAATCTTATTTTTTCTTCTCAAATAGCT
AATTTTGGAGTTGAAATTTGTGAAGATTTATGGGTAACTATACCTCCTAGTTCATACCTTTCTCTACTTGGAGCACATAT
AATAGGAAACTTATCTGCTTCAAATGAATTAGTAAGTAAAAAGGATTATAGAAAAAGCCTCGTTTCAAACCAAAGCGCTA
GATGCCTTTGTAGTTATATATATGCTTCTGCTGGAGTGCATGAATCATCTACCGACTTACTATTTAGCGGACATTTAATG
ATAAGTGAAAATGGTTCTATTTTAAAAGAAAATGAAAGGTTTCAAAGAGAAAACGAAGTTATTTACTCTTGTGTAGATAT
ATTTAGACTTAAATCTGAAAGACTAAAAAATCTTAGTTTTAGAGATGCCACTAAATTCACAGGAAAAAAACCTTATATAA
TAAACTTTAAATTTGGTTGTACTAATATCAGCCACTTTGATAGATTTATAGATAAACATCCTTTTGTACCTTCATCAAAA
TATGAACGCGAAGAAAGATGTAAAGAAATATTTAATATACAAAGTGCTGCTTTAGCAAAAAGATTTGAACATACTGGCTC
TAAAAAAGCTGTAATTGGAATCTCTGGCGGACTAGATTCTACTCTTGCTCTTCTTGTTGTAGTTAAAACCTTTGAATTAC
TTAATTTAGATAAGCATAATATAGTTACTATAACAATGCCGGGATTTGGTACAACAGATAGAACTTATAATAATGCATTA
ACTTTATGCAAAGAACTAAATTGTGATTTAAGAGAAATAAATATAGTAGATGCTTCTCTTCAACATTTTAAAGATATAGG
GCATGATAAAAATATTCATGACGTTACTTATGAAAATGTTCAGGCAAGAGAAAGAACTCAAATTTTAATGGATTTAGCTA
ATAAAGAACAAGGCCTTTTAATAGGAACTGGTGATTTATCTGAACTAGCTCTTGGATGGTGTACTTATAACGGTGATCAT
ATGTCAATGTATTCAGTAAATCCATCTATACCAAAAACATTAGTTAGATATTTAGTCAGATATGTTGCAGAAAAAGAATC
TAATTCAGAAGTTAGCAAAACACTTATTGATATCTTAGATACTCCTGTAAGTCCAGAATTACTTCCTAAGGATGCAAATG
GAGAAATAACACAAAAAACTGAAGATATAGTAGGACCTTATGAATTACATGATTTCTTCTTATATCACTTCATTAAAAAT
GGATCATCAAAAGATAGAATTTTGTTTTTAGCTAAGGAAGCCTTTAAAGATGATTATAGTTATGAAGAAATTGAAAAATG
GCTTAATAAATTTATGTGGAGATTTTTTACTCAACAATTTAAAAGAAGTGCCTTACCAGACGGCCCCAAAGTAGGAAGTA
TCTCTTTAAGCCCTAGAGGTGATTGGAGAATGCCTTCAGATGCAGAACCTTGGAAATAA

Upstream 100 bases:

>100_bases
GCAATTGATTTTATATAATTTTCAGCATATTATGATTATATATACAAATGCTATTTGGTTATACTAAAAGCATATACTAA
AAAATTGGAGTTGATCTAAT

Downstream 100 bases:

>100_bases
TTATAAATTCATCAACATTTTTGTAATTATGTTATTAATTTAGTAATAAATATATACTTTTTATGCTTAACTATAGAATT
TTTGTTTTGATAGTGTTAGA

Product: glutamine-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MDFIKVASACPKTKVGDIDYNISNILKCIDDAKNNNSKFIVFPELCITSYTCGDLFLNDTLLNKSITGINQILNATEDCD
MLIALGAPLLINNVLYNCAYLLFKGKILGIVPKSYIPNYSEFYEKRWFTEGLSLETQEIDLPIQKNVPFGANLIFSSQIA
NFGVEICEDLWVTIPPSSYLSLLGAHIIGNLSASNELVSKKDYRKSLVSNQSARCLCSYIYASAGVHESSTDLLFSGHLM
ISENGSILKENERFQRENEVIYSCVDIFRLKSERLKNLSFRDATKFTGKKPYIINFKFGCTNISHFDRFIDKHPFVPSSK
YEREERCKEIFNIQSAALAKRFEHTGSKKAVIGISGGLDSTLALLVVVKTFELLNLDKHNIVTITMPGFGTTDRTYNNAL
TLCKELNCDLREINIVDASLQHFKDIGHDKNIHDVTYENVQARERTQILMDLANKEQGLLIGTGDLSELALGWCTYNGDH
MSMYSVNPSIPKTLVRYLVRYVAEKESNSEVSKTLIDILDTPVSPELLPKDANGEITQKTEDIVGPYELHDFFLYHFIKN
GSSKDRILFLAKEAFKDDYSYEEIEKWLNKFMWRFFTQQFKRSALPDGPKVGSISLSPRGDWRMPSDAEPWK

Sequences:

>Translated_632_residues
MDFIKVASACPKTKVGDIDYNISNILKCIDDAKNNNSKFIVFPELCITSYTCGDLFLNDTLLNKSITGINQILNATEDCD
MLIALGAPLLINNVLYNCAYLLFKGKILGIVPKSYIPNYSEFYEKRWFTEGLSLETQEIDLPIQKNVPFGANLIFSSQIA
NFGVEICEDLWVTIPPSSYLSLLGAHIIGNLSASNELVSKKDYRKSLVSNQSARCLCSYIYASAGVHESSTDLLFSGHLM
ISENGSILKENERFQRENEVIYSCVDIFRLKSERLKNLSFRDATKFTGKKPYIINFKFGCTNISHFDRFIDKHPFVPSSK
YEREERCKEIFNIQSAALAKRFEHTGSKKAVIGISGGLDSTLALLVVVKTFELLNLDKHNIVTITMPGFGTTDRTYNNAL
TLCKELNCDLREINIVDASLQHFKDIGHDKNIHDVTYENVQARERTQILMDLANKEQGLLIGTGDLSELALGWCTYNGDH
MSMYSVNPSIPKTLVRYLVRYVAEKESNSEVSKTLIDILDTPVSPELLPKDANGEITQKTEDIVGPYELHDFFLYHFIKN
GSSKDRILFLAKEAFKDDYSYEEIEKWLNKFMWRFFTQQFKRSALPDGPKVGSISLSPRGDWRMPSDAEPWK
>Mature_632_residues
MDFIKVASACPKTKVGDIDYNISNILKCIDDAKNNNSKFIVFPELCITSYTCGDLFLNDTLLNKSITGINQILNATEDCD
MLIALGAPLLINNVLYNCAYLLFKGKILGIVPKSYIPNYSEFYEKRWFTEGLSLETQEIDLPIQKNVPFGANLIFSSQIA
NFGVEICEDLWVTIPPSSYLSLLGAHIIGNLSASNELVSKKDYRKSLVSNQSARCLCSYIYASAGVHESSTDLLFSGHLM
ISENGSILKENERFQRENEVIYSCVDIFRLKSERLKNLSFRDATKFTGKKPYIINFKFGCTNISHFDRFIDKHPFVPSSK
YEREERCKEIFNIQSAALAKRFEHTGSKKAVIGISGGLDSTLALLVVVKTFELLNLDKHNIVTITMPGFGTTDRTYNNAL
TLCKELNCDLREINIVDASLQHFKDIGHDKNIHDVTYENVQARERTQILMDLANKEQGLLIGTGDLSELALGWCTYNGDH
MSMYSVNPSIPKTLVRYLVRYVAEKESNSEVSKTLIDILDTPVSPELLPKDANGEITQKTEDIVGPYELHDFFLYHFIKN
GSSKDRILFLAKEAFKDDYSYEEIEKWLNKFMWRFFTQQFKRSALPDGPKVGSISLSPRGDWRMPSDAEPWK

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=608, Percent_Identity=24.8355263157895, Blast_Score=129, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17538756, Length=685, Percent_Identity=24.0875912408759, Blast_Score=124, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321865, Length=590, Percent_Identity=25.5932203389831, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24641841, Length=669, Percent_Identity=24.3647234678625, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24641843, Length=669, Percent_Identity=24.3647234678625, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 71630; Mature: 71630

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFIKVASACPKTKVGDIDYNISNILKCIDDAKNNNSKFIVFPELCITSYTCGDLFLNDT
CCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECHHHHCCCCCCHHHHHHH
LLNKSITGINQILNATEDCDMLIALGAPLLINNVLYNCAYLLFKGKILGIVPKSYIPNYS
HHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCHH
EFYEKRWFTEGLSLETQEIDLPIQKNVPFGANLIFSSQIANFGVEICEDLWVTIPPSSYL
HHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCEEECCCHHHH
SLLGAHIIGNLSASNELVSKKDYRKSLVSNQSARCLCSYIYASAGVHESSTDLLFSGHLM
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
ISENGSILKENERFQRENEVIYSCVDIFRLKSERLKNLSFRDATKFTGKKPYIINFKFGC
EECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEEECC
TNISHFDRFIDKHPFVPSSKYEREERCKEIFNIQSAALAKRFEHTGSKKAVIGISGGLDS
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
TLALLVVVKTFELLNLDKHNIVTITMPGFGTTDRTYNNALTLCKELNCDLREINIVDASL
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCHHEEEEEHHHH
QHFKDIGHDKNIHDVTYENVQARERTQILMDLANKEQGLLIGTGDLSELALGWCTYNGDH
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHEEHEEECCCE
MSMYSVNPSIPKTLVRYLVRYVAEKESNSEVSKTLIDILDTPVSPELLPKDANGEITQKT
EEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
EDIVGPYELHDFFLYHFIKNGSSKDRILFLAKEAFKDDYSYEEIEKWLNKFMWRFFTQQF
HHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KRSALPDGPKVGSISLSPRGDWRMPSDAEPWK
HHHCCCCCCCCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDFIKVASACPKTKVGDIDYNISNILKCIDDAKNNNSKFIVFPELCITSYTCGDLFLNDT
CCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECHHHHCCCCCCHHHHHHH
LLNKSITGINQILNATEDCDMLIALGAPLLINNVLYNCAYLLFKGKILGIVPKSYIPNYS
HHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCHHHCCCHH
EFYEKRWFTEGLSLETQEIDLPIQKNVPFGANLIFSSQIANFGVEICEDLWVTIPPSSYL
HHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCEEECCCHHHH
SLLGAHIIGNLSASNELVSKKDYRKSLVSNQSARCLCSYIYASAGVHESSTDLLFSGHLM
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
ISENGSILKENERFQRENEVIYSCVDIFRLKSERLKNLSFRDATKFTGKKPYIINFKFGC
EECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEEECC
TNISHFDRFIDKHPFVPSSKYEREERCKEIFNIQSAALAKRFEHTGSKKAVIGISGGLDS
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
TLALLVVVKTFELLNLDKHNIVTITMPGFGTTDRTYNNALTLCKELNCDLREINIVDASL
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCHHEEEEEHHHH
QHFKDIGHDKNIHDVTYENVQARERTQILMDLANKEQGLLIGTGDLSELALGWCTYNGDH
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHEEHEEECCCE
MSMYSVNPSIPKTLVRYLVRYVAEKESNSEVSKTLIDILDTPVSPELLPKDANGEITQKT
EEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
EDIVGPYELHDFFLYHFIKNGSSKDRILFLAKEAFKDDYSYEEIEKWLNKFMWRFFTQQF
HHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
KRSALPDGPKVGSISLSPRGDWRMPSDAEPWK
HHHCCCCCCCCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]