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The map label for this gene is pyrE [H]

Identifier: 187934364

GI number: 187934364

Start: 2685913

End: 2686587

Strand: Reverse

Name: pyrE [H]

Synonym: CLL_A2575

Alternate gene names: 187934364

Gene position: 2686587-2685913 (Counterclockwise)

Preceding gene: 187933348

Following gene: 187933107

Centisome position: 70.69

GC content: 28.89

Gene sequence:

>675_bases
ATGGAAGCATATAAAAAAGAGTTTATTGAATTTATGATAGAGTGTGGAGTTTTAACTTTTGGAGATTTTGTAACTAAGAG
TGGTAGAAATACACCTTTCTTTGTTAATACAGGAAATTATAAGACAGGAAGTCAATTAAAAAGATTAGGTGAATATTATG
CTGAAGCTATAAAGGCAAATTATAAAGATGATTATAATATAGTGTTTGGCCCAGCATATAAGGGAATCCCTTTAAGTGTT
ACAGTTACAATGGCTTTAAGTGATAAGTACGGAATAGATGTAAGTTATTGTTCAAATAGAAAAGAGATTAAGGATCATGG
TGATACAGGAATACTTCTTGGAAGTAAATTAAATGATGGAGATAAGGTTTTAATAGTAGAGGATGTAACTACATCAGGAA
AATCTATTTATGAAACAATGCCAATTATAAAAGAACAAGGAAATGTAGATGTAGTAGGACTTGTTATATCTGTTAATAGA
ATGGAAAAAGGACAAGGAGAAAAATCAGCATTAGTAGAATTAGAAGAAAAGTATGGTTTTAAATCTTGCGCAATAGTTAC
AATGACTGAAGTTGTAAAGTATTTGTATAATAAAGAAGTTAACGGAAAAGTAATAATTAATGATGAGGTTAAAACTAGAA
TAGATGAATATTACAAAGAATATGGTGCTAAGTAG

Upstream 100 bases:

>100_bases
TTTAGTATTCCTAAGTTCAATTACTCAGTCCACTTTGCTTATGCATATATAAACACTAGCATATATTAAAATACATTGTG
AATATTGGGAGGAATAGAAA

Downstream 100 bases:

>100_bases
ATTAGATTTTTAGAATTTTTGAGGTGAGTTTAAGGTTTTTAGTTCGATATTTGAAGTTTGATTTTTATAGAAGTTACTAT
GTATAATATTAATAAGAAAA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MEAYKKEFIEFMIECGVLTFGDFVTKSGRNTPFFVNTGNYKTGSQLKRLGEYYAEAIKANYKDDYNIVFGPAYKGIPLSV
TVTMALSDKYGIDVSYCSNRKEIKDHGDTGILLGSKLNDGDKVLIVEDVTTSGKSIYETMPIIKEQGNVDVVGLVISVNR
MEKGQGEKSALVELEEKYGFKSCAIVTMTEVVKYLYNKEVNGKVIINDEVKTRIDEYYKEYGAK

Sequences:

>Translated_224_residues
MEAYKKEFIEFMIECGVLTFGDFVTKSGRNTPFFVNTGNYKTGSQLKRLGEYYAEAIKANYKDDYNIVFGPAYKGIPLSV
TVTMALSDKYGIDVSYCSNRKEIKDHGDTGILLGSKLNDGDKVLIVEDVTTSGKSIYETMPIIKEQGNVDVVGLVISVNR
MEKGQGEKSALVELEEKYGFKSCAIVTMTEVVKYLYNKEVNGKVIINDEVKTRIDEYYKEYGAK
>Mature_224_residues
MEAYKKEFIEFMIECGVLTFGDFVTKSGRNTPFFVNTGNYKTGSQLKRLGEYYAEAIKANYKDDYNIVFGPAYKGIPLSV
TVTMALSDKYGIDVSYCSNRKEIKDHGDTGILLGSKLNDGDKVLIVEDVTTSGKSIYETMPIIKEQGNVDVVGLVISVNR
MEKGQGEKSALVELEEKYGFKSCAIVTMTEVVKYLYNKEVNGKVIINDEVKTRIDEYYKEYGAK

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=202, Percent_Identity=23.7623762376238, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1790073, Length=198, Percent_Identity=43.9393939393939, Blast_Score=181, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323530, Length=231, Percent_Identity=38.5281385281385, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6323927, Length=230, Percent_Identity=36.9565217391304, Blast_Score=145, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 25120; Mature: 25120

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAYKKEFIEFMIECGVLTFGDFVTKSGRNTPFFVNTGNYKTGSQLKRLGEYYAEAIKAN
CCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCC
YKDDYNIVFGPAYKGIPLSVTVTMALSDKYGIDVSYCSNRKEIKDHGDTGILLGSKLNDG
CCCCCEEEECCCCCCCEEEEEEEEEECCCCCCEEHCCCCCHHHHHCCCCEEEEECCCCCC
DKVLIVEDVTTSGKSIYETMPIIKEQGNVDVVGLVISVNRMEKGQGEKSALVELEEKYGF
CEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEEEEEHHCCCCCCHHHHHHHHHHCCC
KSCAIVTMTEVVKYLYNKEVNGKVIINDEVKTRIDEYYKEYGAK
CHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEAYKKEFIEFMIECGVLTFGDFVTKSGRNTPFFVNTGNYKTGSQLKRLGEYYAEAIKAN
CCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCC
YKDDYNIVFGPAYKGIPLSVTVTMALSDKYGIDVSYCSNRKEIKDHGDTGILLGSKLNDG
CCCCCEEEECCCCCCCEEEEEEEEEECCCCCCEEHCCCCCHHHHHCCCCEEEEECCCCCC
DKVLIVEDVTTSGKSIYETMPIIKEQGNVDVVGLVISVNRMEKGQGEKSALVELEEKYGF
CEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEEEEEHHCCCCCCHHHHHHHHHHCCC
KSCAIVTMTEVVKYLYNKEVNGKVIINDEVKTRIDEYYKEYGAK
CHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA