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The map label for this gene is ywtE [H]

Identifier: 187933103

GI number: 187933103

Start: 3318484

End: 3319350

Strand: Reverse

Name: ywtE [H]

Synonym: CLL_A3193

Alternate gene names: 187933103

Gene position: 3319350-3318484 (Counterclockwise)

Preceding gene: 187935484

Following gene: 187933864

Centisome position: 87.34

GC content: 26.41

Gene sequence:

>867_bases
ATGATAAAATTAATAGTTTCTGATATGGATGGAACACTTTTAAATAGTGAACATAAGATATCAAAAGAAAATTTAGATGC
AATAAAAAAGGCAGAGGAAAGAGGTATACATTTTGCAATAGCTACAGGCAGAGCATATCAAGATGTAAAGCCTTTTATTG
ATGAATGTGACTTAGATTGTCAATGTATACTTATGAATGGTGCAGAGTATCGTGATGAAAATGGAAAGATATTAGAAAAT
ATTTATATCGATAAAGAAAAAACAAAAAGAATTTTAGAGGTTTTAAAAAATAGTGGATTCAGTGTTGAATTGTATACAGA
TAAAGGAATATATACAACGGACACTAAAGAAGAAGCATATCAAGGAATGATATATAGGGTTAAAAGTTTTGATCCACACT
TATCAGAAGAAGAGGTATTAGAGAATGCTAAAGTGCATCCACATCTTTTAAATTTAAAGTATATAGATGATATAAATAGA
TTTTTAGAAAGTGAAATAAATATAGCTAAAGTAATTGCTTTTTATGATGATGTAGATGTAATTAAAAAATTAAAGTTTGA
TATAGAAAAGATAGAAGGATTAATTGTAGCATCAACCTTTGCAACTAACGTAGAAATTAATAATATAGAAGCACAAAAAG
GACTTATATTATCTAAAACTATTGAAAAGATGGGTATAAGTAAAGATGAGGTTATAGTTCTAGGTGATAGTTTTAATGAT
TATTCATTATTTACTGAATTTCCAAAATCTTTTGCTATGGAAAATGCTGTACCAGAAATAAAAGAAATAGCTAAGTACAT
AACTGATAATAATGATAATTCAGGAGTAGCTAAAGCTATTTATAAAGCAATAAATGAAGAAATTTAA

Upstream 100 bases:

>100_bases
TTGCTAAAAAACAAGGAAAAGATACGTATTATATATCTAATGTATAGTTAAATTAACATAAAAGAACTATAATTAATATG
AGAATACAGGAGGTAACATA

Downstream 100 bases:

>100_bases
TATTATATTTTAAAATAAAATGTATATGAAAATGCAAACTAGAATTTTAGTTTGCATTTTTTTGTTGCATAAAAATATTT
AGTATAAAAAGAAAGTGAGA

Product: hydrolase, Cof family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MIKLIVSDMDGTLLNSEHKISKENLDAIKKAEERGIHFAIATGRAYQDVKPFIDECDLDCQCILMNGAEYRDENGKILEN
IYIDKEKTKRILEVLKNSGFSVELYTDKGIYTTDTKEEAYQGMIYRVKSFDPHLSEEEVLENAKVHPHLLNLKYIDDINR
FLESEINIAKVIAFYDDVDVIKKLKFDIEKIEGLIVASTFATNVEINNIEAQKGLILSKTIEKMGISKDEVIVLGDSFND
YSLFTEFPKSFAMENAVPEIKEIAKYITDNNDNSGVAKAIYKAINEEI

Sequences:

>Translated_288_residues
MIKLIVSDMDGTLLNSEHKISKENLDAIKKAEERGIHFAIATGRAYQDVKPFIDECDLDCQCILMNGAEYRDENGKILEN
IYIDKEKTKRILEVLKNSGFSVELYTDKGIYTTDTKEEAYQGMIYRVKSFDPHLSEEEVLENAKVHPHLLNLKYIDDINR
FLESEINIAKVIAFYDDVDVIKKLKFDIEKIEGLIVASTFATNVEINNIEAQKGLILSKTIEKMGISKDEVIVLGDSFND
YSLFTEFPKSFAMENAVPEIKEIAKYITDNNDNSGVAKAIYKAINEEI
>Mature_288_residues
MIKLIVSDMDGTLLNSEHKISKENLDAIKKAEERGIHFAIATGRAYQDVKPFIDECDLDCQCILMNGAEYRDENGKILEN
IYIDKEKTKRILEVLKNSGFSVELYTDKGIYTTDTKEEAYQGMIYRVKSFDPHLSEEEVLENAKVHPHLLNLKYIDDINR
FLESEINIAKVIAFYDDVDVIKKLKFDIEKIEGLIVASTFATNVEINNIEAQKGLILSKTIEKMGISKDEVIVLGDSFND
YSLFTEFPKSFAMENAVPEIKEIAKYITDNNDNSGVAKAIYKAINEEI

Specific function: Unknown

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI1786982, Length=284, Percent_Identity=24.6478873239437, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1787043, Length=286, Percent_Identity=25.1748251748252, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI48994981, Length=266, Percent_Identity=25.187969924812, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI87081790, Length=304, Percent_Identity=25.6578947368421, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 32771; Mature: 32771

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLIVSDMDGTLLNSEHKISKENLDAIKKAEERGIHFAIATGRAYQDVKPFIDECDLDC
CEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCCE
QCILMNGAEYRDENGKILENIYIDKEKTKRILEVLKNSGFSVELYTDKGIYTTDTKEEAY
EEEEECCCCEECCCCCEEEHEECCHHHHHHHHHHHHCCCCEEEEEECCCEEECCCHHHHH
QGMIYRVKSFDPHLSEEEVLENAKVHPHLLNLKYIDDINRFLESEINIAKVIAFYDDVDV
HHHEEEEECCCCCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHCCHHH
IKKLKFDIEKIEGLIVASTFATNVEINNIEAQKGLILSKTIEKMGISKDEVIVLGDSFND
HHHHHHHHHHHCCEEEEEEEECCEEEECEEHHCCCHHHHHHHHHCCCCCCEEEECCCCCC
YSLFTEFPKSFAMENAVPEIKEIAKYITDNNDNSGVAKAIYKAINEEI
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKLIVSDMDGTLLNSEHKISKENLDAIKKAEERGIHFAIATGRAYQDVKPFIDECDLDC
CEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCCE
QCILMNGAEYRDENGKILENIYIDKEKTKRILEVLKNSGFSVELYTDKGIYTTDTKEEAY
EEEEECCCCEECCCCCEEEHEECCHHHHHHHHHHHHCCCCEEEEEECCCEEECCCHHHHH
QGMIYRVKSFDPHLSEEEVLENAKVHPHLLNLKYIDDINRFLESEINIAKVIAFYDDVDV
HHHEEEEECCCCCCCHHHHHHCCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHCCHHH
IKKLKFDIEKIEGLIVASTFATNVEINNIEAQKGLILSKTIEKMGISKDEVIVLGDSFND
HHHHHHHHHHHCCEEEEEEEECCEEEECEEHHCCCHHHHHHHHHCCCCCCEEEECCCCCC
YSLFTEFPKSFAMENAVPEIKEIAKYITDNNDNSGVAKAIYKAINEEI
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]