| Definition | Ralstonia pickettii 12J chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010682 |
| Length | 3,942,557 |
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The map label for this gene is lptD [H]
Identifier: 187927494
GI number: 187927494
Start: 415116
End: 417557
Strand: Direct
Name: lptD [H]
Synonym: Rpic_0391
Alternate gene names: 187927494
Gene position: 415116-417557 (Clockwise)
Preceding gene: 187927489
Following gene: 187927495
Centisome position: 10.53
GC content: 63.1
Gene sequence:
>2442_bases ATGACCGAACCGAACCGAGCCAGGAAGAACCGGCAGCGCGCTGCTGTCGCCGCGCCCGATCAGCGGCCCGCCGACTTGGC CACCATCAGGCGTGGCACGCTGGCATTGCGCCCGCTGGTATTTGCGGTGGCCGGTGTCTGGACGGCGACGTCCGCGGCGC AGAGCACGGGCACAACGCAAGCGACGGCGCCCGCTGTCACTCCGGCCTCGTCGGTGACCGCGCTGGCGACGTCCGGACCG ACCACGCCCGGCGGCAGTCCGCTGGTGCCCAAGATGGCCGAGCCCGTCCAGCGACCGGACAACACTGTCCCCACGTTTAC CGCGGCCGACAAGATGGACGGTCGCACCAACGAAGACATCCACCTGCGCGGTAATGGCGAACTGCGCCGCAACGGCTCGG TGCTGAAGGGCGACACGCTCGATTACAACCAGGACACCGACGTCGCAACCGCAACCGGCAACGTGCGCCTGTTCAAGGGC GGCACGCTGGTCACCGGCCCGGACGCCAAGCTGAAGATTACCGCCAACGAAGGGACCATCAGCACGCCGAGCTACGAGTT CCACTCGAACGGCGGCCACGGCACTGCAGACCGCATCGACTTCGTCGACAAGGACAACAGCCGCATCACGCGCGGCACGT ACACCACGTGCTCGCCCGACAACGTCGACTGGTACTTCAGCGCCAGCCGCATCGACATCGACAGCGACCGCCAGGTCGGC ACGGGCCGCAACGGCGTGCTGCACTTCTTCGGCGTGCCGGTGTTTGCGGCGCCGGTCATGGATTTTCCGCTCAATGACGA TCGGCGCAGCGGTTTCCTGGCACCGGTGTTCGGCTACAACAGCCGCAGCGGGGCCGACGTCACGCTGCCGTACTACTTCA ACATCGCGCCGAATCGCGATCTGACGTTGTATCCGCGTCTGCTGAGCCAGCGCGGCTTGCAGCTCGGCGCGGAATACCGC TATCTCGATACATCGTACAGCGGCGTTCTGCGTGGCGAATTCCTGCCCGATGATCGTGAGGCAGGCCGCAACCGCTGGTC GATTTCGGCAGTACACACACAGCGCCTGGCGCCGGGCCTGACCGGCTACATCAACTACAACAAGGTTTCCGATAACACCT ACCCGGATGACCTCGGCCGCAGCATTGTCACGGCGACGCAGCGGCAGTACATCCAGGAGGGCGGTGTTAGTTACTCGTTC GGCGACTGGGTTGCGCTGGCGCGCGTGCAGAAGTTCCAGACGCTGTCGACCGCCACAACGCCACTCGCAGCACCGTATGA GCGACTGCCGCAGTTGAGCCTGACCTATAACAAGTACGACGTTGGCGGCTTTGACATCAATTTCCTGACCGACTACACGC GGTTTTCGATCCCGACGGCCAATACCGTGCAGGGCGAGCGCCTGTTCATGCAGCCGACGATCAGCTACCCGATCGTCCGC CCCGGCTGGTACGTCACCCCGAAGTTCATCCTGAACACCACCGCATACCGCCTGGACCGCCCGGCCGGCGACACGCAGGA CACGCAGATCAATCGCACGCTTCCGACGTTCTCGCTCGATTCGGGCATGACCTTCGAGCGCGATGCGCCGCTGGTCTCCA AATTGTTCGGCGTGAGCTACATCCAGACGCTGGAGCCGCGCGTGTTCTACGTGTACACGCCGTATCGCGACCAGTCGCAG ATTCCGTTGTTCGATACCGGCCAGTCGGACTACAACCTCGGGCAGATATTTACCGAGAACCCGTACACCGGTTACGACCG CATTGCCGACAACAACAAGGTGACGGCAGGTGTGACCACGCGTTTCATCGAGGCCGAATCAGGCATCGAGCGCCTGCGTG CCACGATTGCGCAGCGCGTCGATCTCGACGGGCAGCGCGTGACGCTGGCGGGTGCGGCCCCGACGTCGGTACGCCGCTAT TCGGACCTTCTGGGTGCGACCACGATCCAGATGTTCCGCGGCATCTTTTTCGATACGAACCTTCAGTACAATCAGGACCT CGACCGCATCATGCGTTCGACGGTCGCGTTCAGCTGGAAGCCGGAAGCCAACAAGGTGATCAACCTCGGCTACCGCTATT ACCGGCAAGACGCCAATACCGGTCAGTTGCCGCTGGAACAGGCCGATATCTCCACGCAGTGGCCGATTACGCGGCGCCTC TACGGCCTGGGTCGCATCGGTTACGACCTGGATGCGCGCAAGCCATCGGATGTGCTGCTCGGCCTCGAATATGTGGCGGA CTGCTGGGTCGGCCGACTGGCGGTACAGCGCTACAGCAATGCGGTTTCGGGCTATACGACGCATATCTTTGCCCAGATCG AATTCAAGGGTTTGTCGAAGGTGGGGAACAATCCGATCGACGTGATTCGTCTGAACGTGCCCGGCTACCAGCCGGTGAAC GCACAGCCGATCACGCCGTCCGTGCTGGATCAATATGAATAA
Upstream 100 bases:
>100_bases GGCCTTGTCCAGGCTGCGTCCGGCGGCGGGATTGACCGCAGTACCGACTGATTTTGTGCCTGTGCGTGCCGTTGCGCGCG CGGCCCTGTTCCCGATCTGC
Downstream 100 bases:
>100_bases CGACATCATGGCTTTCCAATCGACCGCTTTCGCCACGCGTGTGGCTCCTCTGGGCCGCTTGCGTGTTGCCACCGGCGTGC TGCTTGCGCTGTTGGCGGGG
Product: Organic solvent tolerance protein
Products: NA
Alternate protein names: Organic solvent tolerance protein [H]
Number of amino acids: Translated: 813; Mature: 812
Protein sequence:
>813_residues MTEPNRARKNRQRAAVAAPDQRPADLATIRRGTLALRPLVFAVAGVWTATSAAQSTGTTQATAPAVTPASSVTALATSGP TTPGGSPLVPKMAEPVQRPDNTVPTFTAADKMDGRTNEDIHLRGNGELRRNGSVLKGDTLDYNQDTDVATATGNVRLFKG GTLVTGPDAKLKITANEGTISTPSYEFHSNGGHGTADRIDFVDKDNSRITRGTYTTCSPDNVDWYFSASRIDIDSDRQVG TGRNGVLHFFGVPVFAAPVMDFPLNDDRRSGFLAPVFGYNSRSGADVTLPYYFNIAPNRDLTLYPRLLSQRGLQLGAEYR YLDTSYSGVLRGEFLPDDREAGRNRWSISAVHTQRLAPGLTGYINYNKVSDNTYPDDLGRSIVTATQRQYIQEGGVSYSF GDWVALARVQKFQTLSTATTPLAAPYERLPQLSLTYNKYDVGGFDINFLTDYTRFSIPTANTVQGERLFMQPTISYPIVR PGWYVTPKFILNTTAYRLDRPAGDTQDTQINRTLPTFSLDSGMTFERDAPLVSKLFGVSYIQTLEPRVFYVYTPYRDQSQ IPLFDTGQSDYNLGQIFTENPYTGYDRIADNNKVTAGVTTRFIEAESGIERLRATIAQRVDLDGQRVTLAGAAPTSVRRY SDLLGATTIQMFRGIFFDTNLQYNQDLDRIMRSTVAFSWKPEANKVINLGYRYYRQDANTGQLPLEQADISTQWPITRRL YGLGRIGYDLDARKPSDVLLGLEYVADCWVGRLAVQRYSNAVSGYTTHIFAQIEFKGLSKVGNNPIDVIRLNVPGYQPVN AQPITPSVLDQYE
Sequences:
>Translated_813_residues MTEPNRARKNRQRAAVAAPDQRPADLATIRRGTLALRPLVFAVAGVWTATSAAQSTGTTQATAPAVTPASSVTALATSGP TTPGGSPLVPKMAEPVQRPDNTVPTFTAADKMDGRTNEDIHLRGNGELRRNGSVLKGDTLDYNQDTDVATATGNVRLFKG GTLVTGPDAKLKITANEGTISTPSYEFHSNGGHGTADRIDFVDKDNSRITRGTYTTCSPDNVDWYFSASRIDIDSDRQVG TGRNGVLHFFGVPVFAAPVMDFPLNDDRRSGFLAPVFGYNSRSGADVTLPYYFNIAPNRDLTLYPRLLSQRGLQLGAEYR YLDTSYSGVLRGEFLPDDREAGRNRWSISAVHTQRLAPGLTGYINYNKVSDNTYPDDLGRSIVTATQRQYIQEGGVSYSF GDWVALARVQKFQTLSTATTPLAAPYERLPQLSLTYNKYDVGGFDINFLTDYTRFSIPTANTVQGERLFMQPTISYPIVR PGWYVTPKFILNTTAYRLDRPAGDTQDTQINRTLPTFSLDSGMTFERDAPLVSKLFGVSYIQTLEPRVFYVYTPYRDQSQ IPLFDTGQSDYNLGQIFTENPYTGYDRIADNNKVTAGVTTRFIEAESGIERLRATIAQRVDLDGQRVTLAGAAPTSVRRY SDLLGATTIQMFRGIFFDTNLQYNQDLDRIMRSTVAFSWKPEANKVINLGYRYYRQDANTGQLPLEQADISTQWPITRRL YGLGRIGYDLDARKPSDVLLGLEYVADCWVGRLAVQRYSNAVSGYTTHIFAQIEFKGLSKVGNNPIDVIRLNVPGYQPVN AQPITPSVLDQYE >Mature_812_residues TEPNRARKNRQRAAVAAPDQRPADLATIRRGTLALRPLVFAVAGVWTATSAAQSTGTTQATAPAVTPASSVTALATSGPT TPGGSPLVPKMAEPVQRPDNTVPTFTAADKMDGRTNEDIHLRGNGELRRNGSVLKGDTLDYNQDTDVATATGNVRLFKGG TLVTGPDAKLKITANEGTISTPSYEFHSNGGHGTADRIDFVDKDNSRITRGTYTTCSPDNVDWYFSASRIDIDSDRQVGT GRNGVLHFFGVPVFAAPVMDFPLNDDRRSGFLAPVFGYNSRSGADVTLPYYFNIAPNRDLTLYPRLLSQRGLQLGAEYRY LDTSYSGVLRGEFLPDDREAGRNRWSISAVHTQRLAPGLTGYINYNKVSDNTYPDDLGRSIVTATQRQYIQEGGVSYSFG DWVALARVQKFQTLSTATTPLAAPYERLPQLSLTYNKYDVGGFDINFLTDYTRFSIPTANTVQGERLFMQPTISYPIVRP GWYVTPKFILNTTAYRLDRPAGDTQDTQINRTLPTFSLDSGMTFERDAPLVSKLFGVSYIQTLEPRVFYVYTPYRDQSQI PLFDTGQSDYNLGQIFTENPYTGYDRIADNNKVTAGVTTRFIEAESGIERLRATIAQRVDLDGQRVTLAGAAPTSVRRYS DLLGATTIQMFRGIFFDTNLQYNQDLDRIMRSTVAFSWKPEANKVINLGYRYYRQDANTGQLPLEQADISTQWPITRRLY GLGRIGYDLDARKPSDVLLGLEYVADCWVGRLAVQRYSNAVSGYTTHIFAQIEFKGLSKVGNNPIDVIRLNVPGYQPVNA QPITPSVLDQYE
Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]
COG id: COG1452
COG function: function code M; Organic solvent tolerance protein OstA
Gene ontology:
Cell location: Cell outer membrane [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the lptD family [H]
Homologues:
Organism=Escherichia coli, GI1786239, Length=757, Percent_Identity=27.7410832232497, Blast_Score=263, Evalue=3e-71,
Paralogues:
None
Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR020889 - InterPro: IPR005653 - InterPro: IPR007543 [H]
Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]
EC number: NA
Molecular weight: Translated: 90096; Mature: 89964
Theoretical pI: Translated: 8.37; Mature: 8.37
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 1.0 %Met (Translated Protein) 1.2 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 0.9 %Met (Mature Protein) 1.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTEPNRARKNRQRAAVAAPDQRPADLATIRRGTLALRPLVFAVAGVWTATSAAQSTGTTQ CCCCHHHHHHHHHCEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC ATAPAVTPASSVTALATSGPTTPGGSPLVPKMAEPVQRPDNTVPTFTAADKMDGRTNEDI CCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEECCCCCCCCCCEE HLRGNGELRRNGSVLKGDTLDYNQDTDVATATGNVRLFKGGTLVTGPDAKLKITANEGTI EEECCCCEECCCCEEECCCCCCCCCCCEEEECCCEEEEECCEEEECCCCEEEEEECCCCC STPSYEFHSNGGHGTADRIDFVDKDNSRITRGTYTTCSPDNVDWYFSASRIDIDSDRQVG CCCCEEEECCCCCCCCCCEEEECCCCCEEECCEEEECCCCCCEEEEEECEEECCCCCCCC TGRNGVLHFFGVPVFAAPVMDFPLNDDRRSGFLAPVFGYNSRSGADVTLPYYFNIAPNRD CCCCCEEEEECCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEEECCCCC LTLYPRLLSQRGLQLGAEYRYLDTSYSGVLRGEFLPDDREAGRNRWSISAVHTQRLAPGL CEEHHHHHHHCCHHCCCCEEEECCCCCCEEECCCCCCHHHHCCCEEEEEEEHHHHHCCCC TGYINYNKVSDNTYPDDLGRSIVTATQRQYIQEGGVSYSFGDWVALARVQKFQTLSTATT EEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHCCC PLAAPYERLPQLSLTYNKYDVGGFDINFLTDYTRFSIPTANTVQGERLFMQPTISYPIVR CCCCCHHHCCCEEEEEEEEECCCEEEEEEECCCEEECCCCCCCCCCEEEECCCCCCCEEC PGWYVTPKFILNTTAYRLDRPAGDTQDTQINRTLPTFSLDSGMTFERDAPLVSKLFGVSY CCCEECCHHEECCHHHEECCCCCCCCCCEEECCCCCEECCCCCCCCCCCHHHHHHHHHHH IQTLEPRVFYVYTPYRDQSQIPLFDTGQSDYNLGQIFTENPYTGYDRIADNNKVTAGVTT HHHCCCEEEEEECCCCCCCCCCEEECCCCCCCCCEEEECCCCCCHHHHCCCCEEEECEEE RFIEAESGIERLRATIAQRVDLDGQRVTLAGAAPTSVRRYSDLLGATTIQMFRGIFFDTN EEEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEECC LQYNQDLDRIMRSTVAFSWKPEANKVINLGYRYYRQDANTGQLPLEQADISTQWPITRRL CCCCHHHHHHHHHHEEEEECCCCCCEEECCHHEEECCCCCCCCCCHHCCCCCCCCHHHHH YGLGRIGYDLDARKPSDVLLGLEYVADCWVGRLAVQRYSNAVSGYTTHIFAQIEFKGLSK HHCCCCCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEHHH VGNNPIDVIRLNVPGYQPVNAQPITPSVLDQYE CCCCCEEEEEEECCCCCCCCCCCCCHHHHHCCC >Mature Secondary Structure TEPNRARKNRQRAAVAAPDQRPADLATIRRGTLALRPLVFAVAGVWTATSAAQSTGTTQ CCCHHHHHHHHHCEEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC ATAPAVTPASSVTALATSGPTTPGGSPLVPKMAEPVQRPDNTVPTFTAADKMDGRTNEDI CCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEECCCCCCCCCCEE HLRGNGELRRNGSVLKGDTLDYNQDTDVATATGNVRLFKGGTLVTGPDAKLKITANEGTI EEECCCCEECCCCEEECCCCCCCCCCCEEEECCCEEEEECCEEEECCCCEEEEEECCCCC STPSYEFHSNGGHGTADRIDFVDKDNSRITRGTYTTCSPDNVDWYFSASRIDIDSDRQVG CCCCEEEECCCCCCCCCCEEEECCCCCEEECCEEEECCCCCCEEEEEECEEECCCCCCCC TGRNGVLHFFGVPVFAAPVMDFPLNDDRRSGFLAPVFGYNSRSGADVTLPYYFNIAPNRD CCCCCEEEEECCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEEECCCCC LTLYPRLLSQRGLQLGAEYRYLDTSYSGVLRGEFLPDDREAGRNRWSISAVHTQRLAPGL CEEHHHHHHHCCHHCCCCEEEECCCCCCEEECCCCCCHHHHCCCEEEEEEEHHHHHCCCC TGYINYNKVSDNTYPDDLGRSIVTATQRQYIQEGGVSYSFGDWVALARVQKFQTLSTATT EEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHCCC PLAAPYERLPQLSLTYNKYDVGGFDINFLTDYTRFSIPTANTVQGERLFMQPTISYPIVR CCCCCHHHCCCEEEEEEEEECCCEEEEEEECCCEEECCCCCCCCCCEEEECCCCCCCEEC PGWYVTPKFILNTTAYRLDRPAGDTQDTQINRTLPTFSLDSGMTFERDAPLVSKLFGVSY CCCEECCHHEECCHHHEECCCCCCCCCCEEECCCCCEECCCCCCCCCCCHHHHHHHHHHH IQTLEPRVFYVYTPYRDQSQIPLFDTGQSDYNLGQIFTENPYTGYDRIADNNKVTAGVTT HHHCCCEEEEEECCCCCCCCCCEEECCCCCCCCCEEEECCCCCCHHHHCCCCEEEECEEE RFIEAESGIERLRATIAQRVDLDGQRVTLAGAAPTSVRRYSDLLGATTIQMFRGIFFDTN EEEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEECC LQYNQDLDRIMRSTVAFSWKPEANKVINLGYRYYRQDANTGQLPLEQADISTQWPITRRL CCCCHHHHHHHHHHEEEEECCCCCCEEECCHHEEECCCCCCCCCCHHCCCCCCCCHHHHH YGLGRIGYDLDARKPSDVLLGLEYVADCWVGRLAVQRYSNAVSGYTTHIFAQIEFKGLSK HHCCCCCCCCCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEHHH VGNNPIDVIRLNVPGYQPVNAQPITPSVLDQYE CCCCCEEEEEEECCCCCCCCCCCCCHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 11823852 [H]