| Definition | Ralstonia pickettii 12J chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010682 |
| Length | 3,942,557 |
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The map label for this gene is purH [H]
Identifier: 187927483
GI number: 187927483
Start: 402754
End: 404328
Strand: Reverse
Name: purH [H]
Synonym: Rpic_0380
Alternate gene names: 187927483
Gene position: 404328-402754 (Counterclockwise)
Preceding gene: 187927484
Following gene: 187927482
Centisome position: 10.26
GC content: 64.83
Gene sequence:
>1575_bases ATGATCCAGCAAGCCCTGCTTTCCGTTTCCGACAAGACCGGCATCGTCGACTTTGCCCGTGCCCTGCACGAGCGCGGCGT CAAGCTCCTCTCCACCGGCGGCACCGCCAAGCTGCTCGCCGAGTCCAGCCTGCCCGTGACGGAAGTGGCGGACTACACCG GCTTTCCGGAAATGCTCGACGGCCGCGTCAAGACGCTGCACCCGAAGGTGCACGGCGGCATCCTGGCCCGTCGCGACCTG CCCGAGCACATGGCCGCACTGTCCGAACACAGCATCCCGACCATCGACCTTCTGGTGGTGAACCTGTACCCGTTCCAGCA GACGGTGGCCAAGGACGAATGCTCGCTGGCCGACGCCATCGAGAACATCGACATCGGCGGCCCGACCATGCTGCGCTCGG CGGCCAAGAACCACCGCGATGTGACAGTCATCGTTGACCCGGCCGACTACGCCACCGTGCTGGCCGAAATGCAGGCCAAC AACAACACGGTCGGCTACGAAACCAACTTCATGCTGGCCAAGAAGGTGTTCGCGCACACCGCGCAGTACGACGGCGCCAT CACCAACTACCTGACGAGCCTCGGCGCCGACAAGTCGCACAGCACGCGCAGCGCCTATCCGCAGACGCTGAACCTCGCCT TCGACAAGGTGCAGGAGATGCGTTACGGCGAAAACCCGCATCAGTCCGCCGCGTTCTACCGCGACCTGAAAGCCGTCGAC GGCGCGCTCGCCAATTACAAGCAACTGCAGGGCAAGGAGCTGTCGTACAACAACATCGCCGACGCCGATGCCGCGTGGGA ATGCGTGAAGTCGTTCGATGCGGCCCACGGCGCTGCATGCGTCATCATCAAGCACGCCAACCCGTGCGGCGTGGCCATCG GCGGCACCGCGCAGGAAGCGTATGAGAAGGCCTTCAAGACCGACTCGACCTCGGCCTTCGGCGGCATCATCGCCTTCAAC GTGCCGCTGGATGAAGCAGCCGCACAGGTGGTGGCCAAGCAGTTCGTGGAAGTGCTGATCGCCCCGGGCTTCTCGGAAGG CGCACGTGCCGTGTTCGCCGCCAAGCAAAACGTGCGTGTGCTGGAGATTCCGTTGGGCAAGGGCGTGAATGCGTACGACT TCAAGCGCGTTGGCGGCGGCCTGCTGGTGCAGAGCCCGGACGCCAAGAACGTGCAGCCGAGCGAGCTGCGCGTGGTTACC AAGCGCCACCCCACGCCAAAGGAAATGGACGACCTGATGTTTGCCTGGCGCGTCGCCAAGTTCGTCAAGTCCAACGCCAT CGTGTTCTGCGGCGGCGGCATGACGCTGGGTGTGGGCGCCGGCCAGATGAGCCGTGTGGACTCGGCCCGCATCGCCAGCA TCAAGGCACAGAACGCAGGCCTGACGCTGGCCGGTTCGGCTGTGGCATCGGATGCGTTCTTCCCGTTTCGCGACGGCCTG GACGTGGTGGTCGATGCTGGCGCCTCGTGCGTGATCCAGCCGGGCGGCTCGGTACGCGACGATGAAGTGATCGCCGCCGC CGACGAACGCAACGTGGCGATGATCTTCACGGGCACGCGCCACTTCCGCCACTAA
Upstream 100 bases:
>100_bases CTGAGGTTGCGGGCCGGCCTTTGAACCGATTTCCTTTCTGACGGCGCCTCCTGCCAGTTCGGCAAACCCAGCGCCACACC CGCCCACCGGCGTTTCCACC
Downstream 100 bases:
>100_bases GCAACATCGTCCACGCGACGAACGAACGGGGCAAGGCGTAAACTGGCGCCTTGCCCCGTTTCCATTTCTGCCCACTTCCT TCATGCGCATCCTCGGCATC
Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
Products: NA
Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]
Number of amino acids: Translated: 524; Mature: 524
Protein sequence:
>524_residues MIQQALLSVSDKTGIVDFARALHERGVKLLSTGGTAKLLAESSLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL PEHMAALSEHSIPTIDLLVVNLYPFQQTVAKDECSLADAIENIDIGGPTMLRSAAKNHRDVTVIVDPADYATVLAEMQAN NNTVGYETNFMLAKKVFAHTAQYDGAITNYLTSLGADKSHSTRSAYPQTLNLAFDKVQEMRYGENPHQSAAFYRDLKAVD GALANYKQLQGKELSYNNIADADAAWECVKSFDAAHGAACVIIKHANPCGVAIGGTAQEAYEKAFKTDSTSAFGGIIAFN VPLDEAAAQVVAKQFVEVLIAPGFSEGARAVFAAKQNVRVLEIPLGKGVNAYDFKRVGGGLLVQSPDAKNVQPSELRVVT KRHPTPKEMDDLMFAWRVAKFVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGL DVVVDAGASCVIQPGGSVRDDEVIAAADERNVAMIFTGTRHFRH
Sequences:
>Translated_524_residues MIQQALLSVSDKTGIVDFARALHERGVKLLSTGGTAKLLAESSLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL PEHMAALSEHSIPTIDLLVVNLYPFQQTVAKDECSLADAIENIDIGGPTMLRSAAKNHRDVTVIVDPADYATVLAEMQAN NNTVGYETNFMLAKKVFAHTAQYDGAITNYLTSLGADKSHSTRSAYPQTLNLAFDKVQEMRYGENPHQSAAFYRDLKAVD GALANYKQLQGKELSYNNIADADAAWECVKSFDAAHGAACVIIKHANPCGVAIGGTAQEAYEKAFKTDSTSAFGGIIAFN VPLDEAAAQVVAKQFVEVLIAPGFSEGARAVFAAKQNVRVLEIPLGKGVNAYDFKRVGGGLLVQSPDAKNVQPSELRVVT KRHPTPKEMDDLMFAWRVAKFVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGL DVVVDAGASCVIQPGGSVRDDEVIAAADERNVAMIFTGTRHFRH >Mature_524_residues MIQQALLSVSDKTGIVDFARALHERGVKLLSTGGTAKLLAESSLPVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDL PEHMAALSEHSIPTIDLLVVNLYPFQQTVAKDECSLADAIENIDIGGPTMLRSAAKNHRDVTVIVDPADYATVLAEMQAN NNTVGYETNFMLAKKVFAHTAQYDGAITNYLTSLGADKSHSTRSAYPQTLNLAFDKVQEMRYGENPHQSAAFYRDLKAVD GALANYKQLQGKELSYNNIADADAAWECVKSFDAAHGAACVIIKHANPCGVAIGGTAQEAYEKAFKTDSTSAFGGIIAFN VPLDEAAAQVVAKQFVEVLIAPGFSEGARAVFAAKQNVRVLEIPLGKGVNAYDFKRVGGGLLVQSPDAKNVQPSELRVVT KRHPTPKEMDDLMFAWRVAKFVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGL DVVVDAGASCVIQPGGSVRDDEVIAAADERNVAMIFTGTRHFRH
Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]
COG id: COG0138
COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the purH family [H]
Homologues:
Organism=Homo sapiens, GI20127454, Length=486, Percent_Identity=36.6255144032922, Blast_Score=269, Evalue=6e-72, Organism=Escherichia coli, GI1790439, Length=530, Percent_Identity=61.5094339622641, Blast_Score=641, Evalue=0.0, Organism=Caenorhabditis elegans, GI71985564, Length=609, Percent_Identity=32.8407224958949, Blast_Score=278, Evalue=5e-75, Organism=Caenorhabditis elegans, GI71985574, Length=314, Percent_Identity=27.0700636942675, Blast_Score=86, Evalue=4e-17, Organism=Caenorhabditis elegans, GI71985556, Length=130, Percent_Identity=34.6153846153846, Blast_Score=74, Evalue=1e-13, Organism=Saccharomyces cerevisiae, GI6323768, Length=480, Percent_Identity=35.4166666666667, Blast_Score=259, Evalue=5e-70, Organism=Saccharomyces cerevisiae, GI6323056, Length=488, Percent_Identity=34.4262295081967, Blast_Score=253, Evalue=4e-68, Organism=Drosophila melanogaster, GI24649832, Length=483, Percent_Identity=36.6459627329193, Blast_Score=259, Evalue=5e-69,
Paralogues:
None
Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002695 - InterPro: IPR013982 - InterPro: IPR016193 - InterPro: IPR011607 [H]
Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]
EC number: =2.1.2.3; =3.5.4.10 [H]
Molecular weight: Translated: 56016; Mature: 56016
Theoretical pI: Translated: 6.51; Mature: 6.51
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 3.4 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 3.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MIQQALLSVSDKTGIVDFARALHERGVKLLSTGGTAKLLAESSLPVTEVADYTGFPEMLD CCHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCCHHHHHHCCCCHHHHC GRVKTLHPKVHGGILARRDLPEHMAALSEHSIPTIDLLVVNLYPFQQTVAKDECSLADAI CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHHHHHHH ENIDIGGPTMLRSAAKNHRDVTVIVDPADYATVLAEMQANNNTVGYETNFMLAKKVFAHT HHCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHH AQYDGAITNYLTSLGADKSHSTRSAYPQTLNLAFDKVQEMRYGENPHQSAAFYRDLKAVD HHCCCHHHHHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH GALANYKQLQGKELSYNNIADADAAWECVKSFDAAHGAACVIIKHANPCGVAIGGTAQEA HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEECCCHHHH YEKAFKTDSTSAFGGIIAFNVPLDEAAAQVVAKQFVEVLIAPGFSEGARAVFAAKQNVRV HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEE LEIPLGKGVNAYDFKRVGGGLLVQSPDAKNVQPSELRVVTKRHPTPKEMDDLMFAWRVAK EEEECCCCCCCHHHHHCCCEEEEECCCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHH FVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGL HHHCCEEEEECCCEEEEECCCHHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCC DVVVDAGASCVIQPGGSVRDDEVIAAADERNVAMIFTGTRHFRH EEEEECCCEEEECCCCCCCCCCEEEEECCCCEEEEEECCHHCCC >Mature Secondary Structure MIQQALLSVSDKTGIVDFARALHERGVKLLSTGGTAKLLAESSLPVTEVADYTGFPEMLD CCHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCCHHHHHHCCCCHHHHC GRVKTLHPKVHGGILARRDLPEHMAALSEHSIPTIDLLVVNLYPFQQTVAKDECSLADAI CCCEEECCHHHCCEEECCCCHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHHHHHHH ENIDIGGPTMLRSAAKNHRDVTVIVDPADYATVLAEMQANNNTVGYETNFMLAKKVFAHT HHCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHH AQYDGAITNYLTSLGADKSHSTRSAYPQTLNLAFDKVQEMRYGENPHQSAAFYRDLKAVD HHCCCHHHHHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH GALANYKQLQGKELSYNNIADADAAWECVKSFDAAHGAACVIIKHANPCGVAIGGTAQEA HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEECCCHHHH YEKAFKTDSTSAFGGIIAFNVPLDEAAAQVVAKQFVEVLIAPGFSEGARAVFAAKQNVRV HHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEE LEIPLGKGVNAYDFKRVGGGLLVQSPDAKNVQPSELRVVTKRHPTPKEMDDLMFAWRVAK EEEECCCCCCCHHHHHCCCEEEEECCCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHHHH FVKSNAIVFCGGGMTLGVGAGQMSRVDSARIASIKAQNAGLTLAGSAVASDAFFPFRDGL HHHCCEEEEECCCEEEEECCCHHHHCCCHHHEEEEECCCCEEEECCHHHCCCCCCCCCCC DVVVDAGASCVIQPGGSVRDDEVIAAADERNVAMIFTGTRHFRH EEEEECCCEEEECCCCCCCCCCEEEEECCCCEEEEEECCHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11823852 [H]