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The map label for this gene is ruvA

Identifier: 187927480

GI number: 187927480

Start: 400226

End: 400807

Strand: Reverse

Name: ruvA

Synonym: Rpic_0377

Alternate gene names: 187927480

Gene position: 400807-400226 (Counterclockwise)

Preceding gene: 187927481

Following gene: 187927479

Centisome position: 10.17

GC content: 67.18

Gene sequence:

>582_bases
ATGATCGGACGCATCGCCGGGACGCTGATCGAAAAGAACCCGCCGCATCTGCTGGTCGATTGCCACGGCGTCGGCTACGA
GATCGACGTGCCGATGAGCACGTTCTATAACCTGCCCGCCACCGGCGAGAAGGTCGTCCTGCTCACGCAGCAGATCGTGC
GCGAGGATGCGCACCTGCTCTACGGTTTCGGCACCGCCGCGGAGCGCGAAACCTTCCGCCAACTCATCAAGATTTCGGGC
ATCGGCGCGCGCATCGCGCTGGCGGTGCTGTCGGGCATGTCGGTGGCCGAACTCGCGCAGGCCGTCACGCTGCAGGAAGC
CGGGCGGCTGACGCGCATCCCGGGCATCGGTAAGAAGACGGCCGAGCGCCTGCTGCTCGAACTCAAGGGCAAGCTCGGCG
CCGACCTGGGTACGGTGCCCGGCGGCCCAGCCGTCTCGGACGACGCGGTCGACGTGCTCAACGCCCTGCTGGCACTAGGG
TATTCCGACAAGGAAGCGGCGCTCGCCATCAAGCAGGTGCCGGCCGGCACGGGCGTGTCCGAGGGAATCAAGCTGGCGCT
CAAGGCGCTGTCCAAGGGCTGA

Upstream 100 bases:

>100_bases
TCTTCGCGCATCGGAACCGTAGCGAGCGGCCGAGCCTGGATCGGGCCGCGCAGTAGGTTTGGGCTACACTGCGTCCCGTT
TTGGAGGAGGAACCCCACCC

Downstream 100 bases:

>100_bases
CGCCCGGGCGCGGCAAGACGGTAGAATGGCCTGCTCGCGCCTTTCCGGCGCCGTCTGCCCGTCATGATCGAAACCGACAA
GCTCGCCGCCAAGGCTGTCT

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MIGRIAGTLIEKNPPHLLVDCHGVGYEIDVPMSTFYNLPATGEKVVLLTQQIVREDAHLLYGFGTAAERETFRQLIKISG
IGARIALAVLSGMSVAELAQAVTLQEAGRLTRIPGIGKKTAERLLLELKGKLGADLGTVPGGPAVSDDAVDVLNALLALG
YSDKEAALAIKQVPAGTGVSEGIKLALKALSKG

Sequences:

>Translated_193_residues
MIGRIAGTLIEKNPPHLLVDCHGVGYEIDVPMSTFYNLPATGEKVVLLTQQIVREDAHLLYGFGTAAERETFRQLIKISG
IGARIALAVLSGMSVAELAQAVTLQEAGRLTRIPGIGKKTAERLLLELKGKLGADLGTVPGGPAVSDDAVDVLNALLALG
YSDKEAALAIKQVPAGTGVSEGIKLALKALSKG
>Mature_193_residues
MIGRIAGTLIEKNPPHLLVDCHGVGYEIDVPMSTFYNLPATGEKVVLLTQQIVREDAHLLYGFGTAAERETFRQLIKISG
IGARIALAVLSGMSVAELAQAVTLQEAGRLTRIPGIGKKTAERLLLELKGKLGADLGTVPGGPAVSDDAVDVLNALLALG
YSDKEAALAIKQVPAGTGVSEGIKLALKALSKG

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=201, Percent_Identity=44.7761194029851, Blast_Score=174, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_RALPJ (B2UFL2)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001897967.1
- ProteinModelPortal:   B2UFL2
- SMR:   B2UFL2
- GeneID:   6289423
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0377
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- ProtClustDB:   PRK00116
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 20120; Mature: 20120

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGRIAGTLIEKNPPHLLVDCHGVGYEIDVPMSTFYNLPATGEKVVLLTQQIVREDAHLL
CCCCHHHHHHCCCCCEEEEEECCCCEEEECCHHHHCCCCCCCCEEHHHHHHHHHCCCCEE
YGFGTAAERETFRQLIKISGIGARIALAVLSGMSVAELAQAVTLQEAGRLTRIPGIGKKT
EECCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCEECCCCCCHHH
AERLLLELKGKLGADLGTVPGGPAVSDDAVDVLNALLALGYSDKEAALAIKQVPAGTGVS
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHH
EGIKLALKALSKG
HHHHHHHHHHHCC
>Mature Secondary Structure
MIGRIAGTLIEKNPPHLLVDCHGVGYEIDVPMSTFYNLPATGEKVVLLTQQIVREDAHLL
CCCCHHHHHHCCCCCEEEEEECCCCEEEECCHHHHCCCCCCCCEEHHHHHHHHHCCCCEE
YGFGTAAERETFRQLIKISGIGARIALAVLSGMSVAELAQAVTLQEAGRLTRIPGIGKKT
EECCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCEECCCCCCHHH
AERLLLELKGKLGADLGTVPGGPAVSDDAVDVLNALLALGYSDKEAALAIKQVPAGTGVS
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHH
EGIKLALKALSKG
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA