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The map label for this gene is ruvB

Identifier: 187927479

GI number: 187927479

Start: 399089

End: 400162

Strand: Reverse

Name: ruvB

Synonym: Rpic_0376

Alternate gene names: 187927479

Gene position: 400162-399089 (Counterclockwise)

Preceding gene: 187927480

Following gene: 187927478

Centisome position: 10.15

GC content: 66.01

Gene sequence:

>1074_bases
ATGATCGAAACCGACAAGCTCGCCGCCAAGGCTGTCTCTGCTGAGCGCATCATCTCAGCCTCCCCCGCCTCCCCCAATGA
GGAGGCGTTCGAGCGCGCGCTGCGCCCCAAGCTGCTCGACGAATACGTCGGTCAGGAAAAGGTGCGCGGCCAGCTCGACA
TCTTCATGACCGCGGCCAAGAACCGCAGCGAAGCGCTGGACCATGTGCTGCTGTTCGGCCCGCCGGGTCTCGGCAAGACG
ACGCTGGCGCACATCATCGCGCGCGAAATGGGCGTCAACCTGCGCCAGACTTCCGGCCCCGTGCTGGAGCGCCCGGGCGA
TCTGGCTGCACTGCTGACCAACCTCGAAGCCAACGACGTCCTCTTCATCGACGAGATTCACCGGCTCTCGCCCGTGGTGG
AAGAAATTCTGTACCCGGCGCTGGAGGACTACCAGATCGACATCATGATCGGCGAGGGCCCGGCGGCGCGCTCGGTCAAG
CTTGATCTGCAGCCCTTTACGCTCGTGGGCGCCACCACGCGCGCCGGCATGCTGACCAACCCGCTGCGCGATCGCTTCGG
GATCGTCGCGCGGCTGGAGTTCTATACGCCTGCCGAACTGGCAAAGATCGTCACGCGCTCATCAGGCCTGCTGAACGCCC
ACATCGTGGACGAGGGGGCGCTGGAAATTGCAAAGCGCTCGCGCGGCACGCCCCGTATCGCCAACCGTCTGCTACGTCGC
GTGCGCGACTTCGCCGAAGTCAAGGCCGACGGCATCATCACCCGCGAAGTCGCCGATGCCGCCCTCGCCATGCTCGACGT
GGACGCGGTCGGCTTCGACCTGATGGACCGCAAGCTGCTCGAGGCGATCCTGCACAAGTTCAACGGCGGCCCGGTCGGCA
TCGACAACCTGGCCGCCGCCATCGGCGAAGAGCGCGACACGATCGAAGACGTGCTCGAGCCCTACCTGATCCAGCAAGGC
TACCTGCAGCGCACGCCACGCGGCCGCGTGGCCACGGCTTCGGCATACCAGCACTTCGGGCTGGGCGCTCCCAAGAGCGG
CCCGGTGCGGGACCTCTGGGACGACAACCAATAA

Upstream 100 bases:

>100_bases
AATCAAGCTGGCGCTCAAGGCGCTGTCCAAGGGCTGACGCCCGGGCGCGGCAAGACGGTAGAATGGCCTGCTCGCGCCTT
TCCGGCGCCGTCTGCCCGTC

Downstream 100 bases:

>100_bases
CCCCCGCGCGCCCCGACATGTCTGCCGCCCCGCACCACCCGAACCGCACGTTCCGATACTACGACTTCATCATGGCGGCC
TTCGTCACAGTGCTGCTGTG

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MIETDKLAAKAVSAERIISASPASPNEEAFERALRPKLLDEYVGQEKVRGQLDIFMTAAKNRSEALDHVLLFGPPGLGKT
TLAHIIAREMGVNLRQTSGPVLERPGDLAALLTNLEANDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVK
LDLQPFTLVGATTRAGMLTNPLRDRFGIVARLEFYTPAELAKIVTRSSGLLNAHIVDEGALEIAKRSRGTPRIANRLLRR
VRDFAEVKADGIITREVADAALAMLDVDAVGFDLMDRKLLEAILHKFNGGPVGIDNLAAAIGEERDTIEDVLEPYLIQQG
YLQRTPRGRVATASAYQHFGLGAPKSGPVRDLWDDNQ

Sequences:

>Translated_357_residues
MIETDKLAAKAVSAERIISASPASPNEEAFERALRPKLLDEYVGQEKVRGQLDIFMTAAKNRSEALDHVLLFGPPGLGKT
TLAHIIAREMGVNLRQTSGPVLERPGDLAALLTNLEANDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVK
LDLQPFTLVGATTRAGMLTNPLRDRFGIVARLEFYTPAELAKIVTRSSGLLNAHIVDEGALEIAKRSRGTPRIANRLLRR
VRDFAEVKADGIITREVADAALAMLDVDAVGFDLMDRKLLEAILHKFNGGPVGIDNLAAAIGEERDTIEDVLEPYLIQQG
YLQRTPRGRVATASAYQHFGLGAPKSGPVRDLWDDNQ
>Mature_357_residues
MIETDKLAAKAVSAERIISASPASPNEEAFERALRPKLLDEYVGQEKVRGQLDIFMTAAKNRSEALDHVLLFGPPGLGKT
TLAHIIAREMGVNLRQTSGPVLERPGDLAALLTNLEANDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVK
LDLQPFTLVGATTRAGMLTNPLRDRFGIVARLEFYTPAELAKIVTRSSGLLNAHIVDEGALEIAKRSRGTPRIANRLLRR
VRDFAEVKADGIITREVADAALAMLDVDAVGFDLMDRKLLEAILHKFNGGPVGIDNLAAAIGEERDTIEDVLEPYLIQQG
YLQRTPRGRVATASAYQHFGLGAPKSGPVRDLWDDNQ

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=334, Percent_Identity=71.5568862275449, Blast_Score=487, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_RALPJ (B2UFL1)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001897966.1
- ProteinModelPortal:   B2UFL1
- SMR:   B2UFL1
- GeneID:   6289422
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0376
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 39070; Mature: 39070

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETDKLAAKAVSAERIISASPASPNEEAFERALRPKLLDEYVGQEKVRGQLDIFMTAAK
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCHHEEEEEHHC
NRSEALDHVLLFGPPGLGKTTLAHIIAREMGVNLRQTSGPVLERPGDLAALLTNLEANDV
CHHHHHCEEEEECCCCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHCCCCCCE
LFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVKLDLQPFTLVGATTRAGMLTN
EEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCEEEEECCHHCCHHHH
PLRDRFGIVARLEFYTPAELAKIVTRSSGLLNAHIVDEGALEIAKRSRGTPRIANRLLRR
HHHHHHCEEEEEECCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCCHHHHHHHHHH
VRDFAEVKADGIITREVADAALAMLDVDAVGFDLMDRKLLEAILHKFNGGPVGIDNLAAA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
IGEERDTIEDVLEPYLIQQGYLQRTPRGRVATASAYQHFGLGAPKSGPVRDLWDDNQ
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIETDKLAAKAVSAERIISASPASPNEEAFERALRPKLLDEYVGQEKVRGQLDIFMTAAK
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCHHEEEEEHHC
NRSEALDHVLLFGPPGLGKTTLAHIIAREMGVNLRQTSGPVLERPGDLAALLTNLEANDV
CHHHHHCEEEEECCCCCCHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHCCCCCCE
LFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVKLDLQPFTLVGATTRAGMLTN
EEHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCEEEEECCHHCCHHHH
PLRDRFGIVARLEFYTPAELAKIVTRSSGLLNAHIVDEGALEIAKRSRGTPRIANRLLRR
HHHHHHCEEEEEECCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCCHHHHHHHHHH
VRDFAEVKADGIITREVADAALAMLDVDAVGFDLMDRKLLEAILHKFNGGPVGIDNLAAA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
IGEERDTIEDVLEPYLIQQGYLQRTPRGRVATASAYQHFGLGAPKSGPVRDLWDDNQ
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA