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The map label for this gene is thlA [H]

Identifier: 187927234

GI number: 187927234

Start: 145295

End: 146476

Strand: Reverse

Name: thlA [H]

Synonym: Rpic_0126

Alternate gene names: 187927234

Gene position: 146476-145295 (Counterclockwise)

Preceding gene: 187927235

Following gene: 187927233

Centisome position: 3.72

GC content: 66.58

Gene sequence:

>1182_bases
ATGTCCGACCGAATCGTCATCGCCTCCGCCGCCCGTACCCCGATGGGCGCCTTCCAGGGCGAACTCGCCAGCCTCACCGC
TCCGCAACTCGGCGCCGCTGCCATTCGCGCGGCGGTCGAACGTGCGGGCTTGAAACCGGAACAGATCGAAGAAGTCATCA
TGGGCTGTGTTCTGCCTGCGGGCCAGGGGCAGGCACCGGCACGCCAGGCCGCATTGGGCGCGGGCCTGCCGCTGTCGGTC
GGCTGCACCACGGTCAACAAGATGTGCGGTTCGGGCATGCGCGCGGCCATGAACGCGTATGACGCACTGCTCGCGGGTTC
GGTCGACGTGGTGGTCGCGGGCGGCATGGAAAGCATGACGAATGCGCCGTACCTGATCCCGAAAGGCCGCGGCGGTTATC
GCATCGGCCACGGCATGATCTACGACCACATGATGCTGGACGGCCTGGAAGACGCATACGACAAGGGCCGCGCCATGGGC
ACCTTCGGCGAGGACTGCGCCGAGAAGTACGGCTTCACGCGCGAAGCCCAGGACCACTTCGCGATGACGTCCGTGCGCCG
CGCGCAGGAGGCCACCGAATCCGGCGCATTCCGCTGGGAAATCACGCCCGTTACCGTGTCGAGCAAAGCGGGCGACACCG
TCATCGACACCGATGAAGGCCCGCGCCGCATCAAGGTCGACAAGATTCCCTCGCTCAAGCCCGCGTTCAAGAAAGACGGC
ACGGTGACGGCCGCGTCGTCTTCGTCGATCAACGATGGGGCGGCTGCGCTGGTGATGATGCGCGAGTCGACTGCCAAGCA
ACTCGGCATCACGCCGCTGGCGGTGATGCTGGGCCACACCACGCACGCACAGGCACCGGGCTGGTTCACGACGGCGCCGG
TCACAGCCATCGACAAGCTGTTCAAGAAGCTCGACTGGAACTCGGGGAACGTCGACCTGTTCGAGATCAACGAGGCGTTT
GCCGTGGTGCCGATGGCCGCGATGCACGACCTGCACATCCCGCACGAGAAGGTGAACGTGAACGGCGGCGCATGCGCGCT
GGGCCATCCGATCGGCGCATCCGGCGCGCGCGTGATCGCGACGCTGCTGGGCGCGCTGCAACACCGTGGCGGCAAGCGCG
GTGTGGCCAGCCTGTGCATCGGCGGCGGTGAAGCCACGGCCGTCGGCATCGAACTCTACTAA

Upstream 100 bases:

>100_bases
GAGATGGACCAACAAGGCCCACCCAAAAAGCAAGAGACACACAACCCCTGCACCAAAAGTGCAGAACATGTCCCAAGCAC
AGTTCCAGGAGATTGCAGCA

Downstream 100 bases:

>100_bases
GGCACGCGCATGACGAACGCAACGTCCCGCCGCACGCGCACCGCGCTCATCCTGGGCGCGTCGCGCGGCATCGGCCTGGA
AACGGTCAAGCAGTATCGCG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSDRIVIASAARTPMGAFQGELASLTAPQLGAAAIRAAVERAGLKPEQIEEVIMGCVLPAGQGQAPARQAALGAGLPLSV
GCTTVNKMCGSGMRAAMNAYDALLAGSVDVVVAGGMESMTNAPYLIPKGRGGYRIGHGMIYDHMMLDGLEDAYDKGRAMG
TFGEDCAEKYGFTREAQDHFAMTSVRRAQEATESGAFRWEITPVTVSSKAGDTVIDTDEGPRRIKVDKIPSLKPAFKKDG
TVTAASSSSINDGAAALVMMRESTAKQLGITPLAVMLGHTTHAQAPGWFTTAPVTAIDKLFKKLDWNSGNVDLFEINEAF
AVVPMAAMHDLHIPHEKVNVNGGACALGHPIGASGARVIATLLGALQHRGGKRGVASLCIGGGEATAVGIELY

Sequences:

>Translated_393_residues
MSDRIVIASAARTPMGAFQGELASLTAPQLGAAAIRAAVERAGLKPEQIEEVIMGCVLPAGQGQAPARQAALGAGLPLSV
GCTTVNKMCGSGMRAAMNAYDALLAGSVDVVVAGGMESMTNAPYLIPKGRGGYRIGHGMIYDHMMLDGLEDAYDKGRAMG
TFGEDCAEKYGFTREAQDHFAMTSVRRAQEATESGAFRWEITPVTVSSKAGDTVIDTDEGPRRIKVDKIPSLKPAFKKDG
TVTAASSSSINDGAAALVMMRESTAKQLGITPLAVMLGHTTHAQAPGWFTTAPVTAIDKLFKKLDWNSGNVDLFEINEAF
AVVPMAAMHDLHIPHEKVNVNGGACALGHPIGASGARVIATLLGALQHRGGKRGVASLCIGGGEATAVGIELY
>Mature_392_residues
SDRIVIASAARTPMGAFQGELASLTAPQLGAAAIRAAVERAGLKPEQIEEVIMGCVLPAGQGQAPARQAALGAGLPLSVG
CTTVNKMCGSGMRAAMNAYDALLAGSVDVVVAGGMESMTNAPYLIPKGRGGYRIGHGMIYDHMMLDGLEDAYDKGRAMGT
FGEDCAEKYGFTREAQDHFAMTSVRRAQEATESGAFRWEITPVTVSSKAGDTVIDTDEGPRRIKVDKIPSLKPAFKKDGT
VTAASSSSINDGAAALVMMRESTAKQLGITPLAVMLGHTTHAQAPGWFTTAPVTAIDKLFKKLDWNSGNVDLFEINEAFA
VVPMAAMHDLHIPHEKVNVNGGACALGHPIGASGARVIATLLGALQHRGGKRGVASLCIGGGEATAVGIELY

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4557237, Length=390, Percent_Identity=46.6666666666667, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI148539872, Length=393, Percent_Identity=44.529262086514, Blast_Score=312, Evalue=4e-85,
Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=36.7609254498715, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI4501853, Length=396, Percent_Identity=37.3737373737374, Blast_Score=219, Evalue=5e-57,
Organism=Homo sapiens, GI4504327, Length=435, Percent_Identity=28.735632183908, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI194353979, Length=396, Percent_Identity=31.3131313131313, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1788554, Length=388, Percent_Identity=49.2268041237113, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI87082165, Length=388, Percent_Identity=41.7525773195876, Blast_Score=311, Evalue=6e-86,
Organism=Escherichia coli, GI1787663, Length=395, Percent_Identity=35.6962025316456, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI48994986, Length=401, Percent_Identity=35.6608478802993, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1788683, Length=416, Percent_Identity=28.3653846153846, Blast_Score=148, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI25147385, Length=385, Percent_Identity=43.1168831168831, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17535921, Length=394, Percent_Identity=44.1624365482233, Blast_Score=305, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI133906874, Length=388, Percent_Identity=38.4020618556701, Blast_Score=271, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17535917, Length=392, Percent_Identity=34.4387755102041, Blast_Score=197, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=31.2056737588652, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6325229, Length=402, Percent_Identity=47.5124378109453, Blast_Score=337, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6322031, Length=393, Percent_Identity=33.8422391857506, Blast_Score=189, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24655093, Length=395, Percent_Identity=45.8227848101266, Blast_Score=340, Evalue=7e-94,
Organism=Drosophila melanogaster, GI24640423, Length=390, Percent_Identity=46.6666666666667, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI17648125, Length=390, Percent_Identity=35.1282051282051, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=30.0469483568075, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40907; Mature: 40776

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRIVIASAARTPMGAFQGELASLTAPQLGAAAIRAAVERAGLKPEQIEEVIMGCVLPA
CCCCEEEEECCCCCCHHHHCHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
GQGQAPARQAALGAGLPLSVGCTTVNKMCGSGMRAAMNAYDALLAGSVDVVVAGGMESMT
CCCCCCHHHHHHCCCCCCEECHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCHHHHC
NAPYLIPKGRGGYRIGHGMIYDHMMLDGLEDAYDKGRAMGTFGEDCAEKYGFTREAQDHF
CCCEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHH
AMTSVRRAQEATESGAFRWEITPVTVSSKAGDTVIDTDEGPRRIKVDKIPSLKPAFKKDG
HHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEECCCCCCEEEECCCCCCCCCCCCCC
TVTAASSSSINDGAAALVMMRESTAKQLGITPLAVMLGHTTHAQAPGWFTTAPVTAIDKL
CEEECCCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHCCCCCCCCCCCEECCHHHHHHHH
FKKLDWNSGNVDLFEINEAFAVVPMAAMHDLHIPHEKVNVNGGACALGHPIGASGARVIA
HHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCHHEEECCCCEEEECCCCCCCHHHHHH
TLLGALQHRGGKRGVASLCIGGGEATAVGIELY
HHHHHHHHCCCCCCHHHEEECCCCEEEEEEEEC
>Mature Secondary Structure 
SDRIVIASAARTPMGAFQGELASLTAPQLGAAAIRAAVERAGLKPEQIEEVIMGCVLPA
CCCEEEEECCCCCCHHHHCHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
GQGQAPARQAALGAGLPLSVGCTTVNKMCGSGMRAAMNAYDALLAGSVDVVVAGGMESMT
CCCCCCHHHHHHCCCCCCEECHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCHHHHC
NAPYLIPKGRGGYRIGHGMIYDHMMLDGLEDAYDKGRAMGTFGEDCAEKYGFTREAQDHF
CCCEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHH
AMTSVRRAQEATESGAFRWEITPVTVSSKAGDTVIDTDEGPRRIKVDKIPSLKPAFKKDG
HHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEECCCCCCEEEECCCCCCCCCCCCCC
TVTAASSSSINDGAAALVMMRESTAKQLGITPLAVMLGHTTHAQAPGWFTTAPVTAIDKL
CEEECCCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHCCCCCCCCCCCEECCHHHHHHHH
FKKLDWNSGNVDLFEINEAFAVVPMAAMHDLHIPHEKVNVNGGACALGHPIGASGARVIA
HHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCHHEEECCCCEEEECCCCCCCHHHHHH
TLLGALQHRGGKRGVASLCIGGGEATAVGIELY
HHHHHHHHCCCCCCHHHEEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]