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The map label for this gene is glmU

Identifier: 187927171

GI number: 187927171

Start: 74849

End: 76216

Strand: Direct

Name: glmU

Synonym: Rpic_0061

Alternate gene names: 187927171

Gene position: 74849-76216 (Clockwise)

Preceding gene: 187927170

Following gene: 187927172

Centisome position: 1.9

GC content: 67.32

Gene sequence:

>1368_bases
GTGAATATCGTCATCCTGGCTGCAGGCATGGGCAAACGCATGCGCTCCGCCCTGCCGAAAGTGCTGCACCCCCTGACCGG
CAAACCGCTGCTGGCGCACGTCATTGAAACCGCGCGCACCATGTCGCCGACGCGCCTGGTGGTGGTGGTCGGACATGGCG
GTGATCGGGTGCGCGAGATGGTTGGCGCGCCCGACATCGCCTTCGCCACGCAAGACAAGCAGCTTGGCACGGGCCACGCC
GTCATGCAGGCCGTCGACCAGCTCGACGAGAGCGTGCCCACCCTGGTGCTCTACGGCGATGTGCCGCTCACCCGCGCCGA
AACGCTGAGCGCGCTGGTAGGCGCCGCCGGCAACGACCACCTGGGCGTGCTGACCGTGCATCTGGGTGACCCGACTGGCT
ACGGCCGCATCGTGCGCGACGCCGCAGGCCGCATCACGCGCATCGTCGAGCAGAAAGACGCCAACGAAACGCAACTCGCC
ATCCATGAGGTGAACACCGGCATCCTGGTGTGCCCGACGGCCAAGCTCAAGAGCTGGCTCGCCACGCTCTCCAACGACAA
CGCGCAGGGCGAGTATTACCTGACCGACGTGATCGAGCGCGCCGCCAGCGAAGGCGTGCCGATCACGTCCGCCCACCCGC
TGGCCGAGTGGGAAACGCTGGGCGTGAACAGCAAGGTGCAACTGGCGGAGCTGGAGCGCATTCACCAGCGCAACCTCGCG
CAGCAACTGCTGGAAGACGGCGTGACGTTGATCGACCCGGCGCGCATCGACGTGCGCGGCAAGCTCACCTGCGGCCGCGA
CGTCGTGATCGACGTCAACTGCATCTTCGAGGGCGACGTCACGCTGGCTGACGGCGTGCGCATCGGCGCCCATACCGTGA
TCCGCGATGCCGCCATCGAGGCCGGCGCCGAGATCCTGCCGTTCTGCCATATCGAGCGTGCCAAGGTTGGCGCCGACTCC
CGCATCGGCCCGTACGCGCGCCTGCGCCCGGGCACCGAGCTGGCCGAAGACGTGCACATCGGTAACTTTGTCGAGGTCAA
GAACAGCCAGATCGCGGCGCACAGCAAGGCCAACCACCTGGCCTATGTGGGCGACGCCACGGTCGGCTCGCGCGTCAACA
TCGGCGCGGGCACCATCACGTGCAACTACGACGGCGCGAACAAATTCCGCACCGTCATCGAAGACGACGCCTTCATCGGT
TCCGACACGCAGCTCGTGGCGCCGGTGCGCGTTGGCAAGGGCGCGACGCTGGGTGCCGGCACCACGCTGACCAAGGATGC
GCCCGAGGGCAAGCTGACGGTCTCGCGGGCGCGGCAGGTCACCATCGACAGCTGGCAGCGCCCGGTCAAGCAGAAGAAGG
AAGGCTGA

Upstream 100 bases:

>100_bases
CATCCTGAGGGGAGGTGGCGGGCGGTGGGTTTCAGGCAGCCATTCCCGGTGCCGGCAACCCGTCGCAGCTATCGCTTCGA
GTCACTCTAGGAACCTCCTT

Downstream 100 bases:

>100_bases
CAGGGTTTCTCCTGCGCCGAACAGCGGATGCCGCTTCGGCACACGAGCATCGATACTGAATTACCGAGTGCGGCGCACAT
TGAACGTGCGGCCACACCCC

Product: UDP-N-acetylglucosamine pyrophosphorylase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MNIVILAAGMGKRMRSALPKVLHPLTGKPLLAHVIETARTMSPTRLVVVVGHGGDRVREMVGAPDIAFATQDKQLGTGHA
VMQAVDQLDESVPTLVLYGDVPLTRAETLSALVGAAGNDHLGVLTVHLGDPTGYGRIVRDAAGRITRIVEQKDANETQLA
IHEVNTGILVCPTAKLKSWLATLSNDNAQGEYYLTDVIERAASEGVPITSAHPLAEWETLGVNSKVQLAELERIHQRNLA
QQLLEDGVTLIDPARIDVRGKLTCGRDVVIDVNCIFEGDVTLADGVRIGAHTVIRDAAIEAGAEILPFCHIERAKVGADS
RIGPYARLRPGTELAEDVHIGNFVEVKNSQIAAHSKANHLAYVGDATVGSRVNIGAGTITCNYDGANKFRTVIEDDAFIG
SDTQLVAPVRVGKGATLGAGTTLTKDAPEGKLTVSRARQVTIDSWQRPVKQKKEG

Sequences:

>Translated_455_residues
MNIVILAAGMGKRMRSALPKVLHPLTGKPLLAHVIETARTMSPTRLVVVVGHGGDRVREMVGAPDIAFATQDKQLGTGHA
VMQAVDQLDESVPTLVLYGDVPLTRAETLSALVGAAGNDHLGVLTVHLGDPTGYGRIVRDAAGRITRIVEQKDANETQLA
IHEVNTGILVCPTAKLKSWLATLSNDNAQGEYYLTDVIERAASEGVPITSAHPLAEWETLGVNSKVQLAELERIHQRNLA
QQLLEDGVTLIDPARIDVRGKLTCGRDVVIDVNCIFEGDVTLADGVRIGAHTVIRDAAIEAGAEILPFCHIERAKVGADS
RIGPYARLRPGTELAEDVHIGNFVEVKNSQIAAHSKANHLAYVGDATVGSRVNIGAGTITCNYDGANKFRTVIEDDAFIG
SDTQLVAPVRVGKGATLGAGTTLTKDAPEGKLTVSRARQVTIDSWQRPVKQKKEG
>Mature_455_residues
MNIVILAAGMGKRMRSALPKVLHPLTGKPLLAHVIETARTMSPTRLVVVVGHGGDRVREMVGAPDIAFATQDKQLGTGHA
VMQAVDQLDESVPTLVLYGDVPLTRAETLSALVGAAGNDHLGVLTVHLGDPTGYGRIVRDAAGRITRIVEQKDANETQLA
IHEVNTGILVCPTAKLKSWLATLSNDNAQGEYYLTDVIERAASEGVPITSAHPLAEWETLGVNSKVQLAELERIHQRNLA
QQLLEDGVTLIDPARIDVRGKLTCGRDVVIDVNCIFEGDVTLADGVRIGAHTVIRDAAIEAGAEILPFCHIERAKVGADS
RIGPYARLRPGTELAEDVHIGNFVEVKNSQIAAHSKANHLAYVGDATVGSRVNIGAGTITCNYDGANKFRTVIEDDAFIG
SDTQLVAPVRVGKGATLGAGTTLTKDAPEGKLTVSRARQVTIDSWQRPVKQKKEG

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=452, Percent_Identity=53.0973451327434, Blast_Score=470, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_RALPJ (B2UD47)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001897658.1
- ProteinModelPortal:   B2UD47
- SMR:   B2UD47
- GeneID:   6288988
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_0061
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- ProtClustDB:   CLSK896503
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 48513; Mature: 48513

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 359-359 BINDING 71-71 BINDING 135-135 BINDING 150-150 BINDING 165-165 BINDING 383-383 BINDING 401-401 BINDING 419-419 BINDING 436-436

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVILAAGMGKRMRSALPKVLHPLTGKPLLAHVIETARTMSPTRLVVVVGHGGDRVREM
CEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHH
VGAPDIAFATQDKQLGTGHAVMQAVDQLDESVPTLVLYGDVPLTRAETLSALVGAAGNDH
HCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCC
LGVLTVHLGDPTGYGRIVRDAAGRITRIVEQKDANETQLAIHEVNTGILVCPTAKLKSWL
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCHHHHHHH
ATLSNDNAQGEYYLTDVIERAASEGVPITSAHPLAEWETLGVNSKVQLAELERIHQRNLA
HHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHH
QQLLEDGVTLIDPARIDVRGKLTCGRDVVIDVNCIFEGDVTLADGVRIGAHTVIRDAAIE
HHHHHCCCEEECCCEEEEEEEEEECCEEEEEEEEEEECCEEEECCEEECHHHHHHHHHHH
AGAEILPFCHIERAKVGADSRIGPYARLRPGTELAEDVHIGNFVEVKNSQIAAHSKANHL
CCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCEEEECCCCCCE
AYVGDATVGSRVNIGAGTITCNYDGANKFRTVIEDDAFIGSDTQLVAPVRVGKGATLGAG
EEEECCCCCCEEECCCEEEEEECCCCHHHHHHHCCCCEECCCCEEEEEEEECCCCEECCC
TTLTKDAPEGKLTVSRARQVTIDSWQRPVKQKKEG
CEECCCCCCCCEEECHHCEEEHHHHHHHHHHCCCC
>Mature Secondary Structure
MNIVILAAGMGKRMRSALPKVLHPLTGKPLLAHVIETARTMSPTRLVVVVGHGGDRVREM
CEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHH
VGAPDIAFATQDKQLGTGHAVMQAVDQLDESVPTLVLYGDVPLTRAETLSALVGAAGNDH
HCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCC
LGVLTVHLGDPTGYGRIVRDAAGRITRIVEQKDANETQLAIHEVNTGILVCPTAKLKSWL
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCHHHHHHH
ATLSNDNAQGEYYLTDVIERAASEGVPITSAHPLAEWETLGVNSKVQLAELERIHQRNLA
HHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHH
QQLLEDGVTLIDPARIDVRGKLTCGRDVVIDVNCIFEGDVTLADGVRIGAHTVIRDAAIE
HHHHHCCCEEECCCEEEEEEEEEECCEEEEEEEEEEECCEEEECCEEECHHHHHHHHHHH
AGAEILPFCHIERAKVGADSRIGPYARLRPGTELAEDVHIGNFVEVKNSQIAAHSKANHL
CCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCEEEECCCCCCE
AYVGDATVGSRVNIGAGTITCNYDGANKFRTVIEDDAFIGSDTQLVAPVRVGKGATLGAG
EEEECCCCCCEEECCCEEEEEECCCCHHHHHHHCCCCEECCCCEEEEEEEECCCCEECCC
TTLTKDAPEGKLTVSRARQVTIDSWQRPVKQKKEG
CEECCCCCCCCEEECHHCEEEHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA