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The map label for this gene is mleN [H]

Identifier: 187918494

GI number: 187918494

Start: 682550

End: 683887

Strand: Direct

Name: mleN [H]

Synonym: BH0638

Alternate gene names: 187918494

Gene position: 682550-683887 (Clockwise)

Preceding gene: 187918493

Following gene: 187918500

Centisome position: 74.0

GC content: 34.6

Gene sequence:

>1338_bases
ATGAACAAAGGTAGTCATATAAGGCCGAATTTTTTAGGGCTTGTTCCTTTCCTTGTTTTTATTATTGTTTATGTGGGAAC
AGGAGTGGCTTTAGAGATGCAAGGTGTAGAGATGGCTTTTTATCAGATGCCACCAATAATTGCTATGTTATTAGCTGTTG
TTACTGCATTCCTTTTATTCAAGGGTTCGTTTATAGAGAAGCTGAATGAATTTATTGAAGGATGCTCACAGACTGATATT
ATATTTATATCCTTCATCTTTCTGATTTCAGGTGCTTTTTCTGCTGTTTGCAAGGAAATAGGTAGCATTGAAACCGTAGC
AAATATTGGACTTAAGTATATACCCGCTAATTTGTTGGTAGCAGGAATATTTTTAATATGCCTTTTCCTTTCTACTGCAA
CTGGTAGTTTCCTTGGAACTGTTGTGGCTGTTACTCCAATTGGATTCGAGATAGCAGGTAAGAGTGGTATTCCATTGCCA
ATGGTTGCGGGAGCTGTGCTTGGGGGCGGTGCTTTTGGTGATAGCATGTCTTTAATATCAGATACAACTATTATTGCAAG
TCGTACGCAAGGAGTTAAAATTATAGATGTTTTTAGGAATGGTGTTTTTTTTACATTTCCTGCATCTATTTTGTCGACTG
TAGCATTTGCTATTTTAGGCTCTTATGTTGGTGGTGTTGGAGTTAATGTTGAGCTTGGTGAGATAAGTTATTTGAAAGTG
ATACCTTACCTTTTTGTTATAATTTTTGCGTTCTTAGGAGTAGATGTATTTTTAGTTTTATTTTTGGGAATATTGATTGC
TGGTGGTATTGGTATTTTAAATGGTGATTTAACTTTACTTTTAATGGCTCAAAAGATTAATGTGGGGCTTTTAGATTTAA
GTGAGATGCTTATTCTTGTTATTTTTACAGGAGGAATTTCTTATATGACTATTAGCCATGGAGGTTTTGAGTGGATCTTA
ACTAAATTGAAGTATCTAGCTAAGTGTAGGAGAAGTGCTGAGTTTACAATTACTTTTTTAATAGTTATGGTAACGGGTTT
TCTTTCAAATAGTGGTCTTGCGATTTTGGTTAATGAGACTGTTACTAAGGGTATATCTGAGGCTAACTCTGTATGTCCTA
AGCGTTGTGCGGCATTACTTTCAATTTCTTCTTGTGCTTTAATTGGTGCTTTACCATATGGTATGCATATGATAAGCGTA
ATGAATATTGCAAAAGGGGCTATATCCCCACTTGATATCATGCCGTTTTTGTTTTATCAAGTGTTTTTAGGTATTATTAT
TTTCTCTATAGCTTTTTTGGGATTGAAAAATCAGTTTTTAAATTCTACTAAACCTTGA

Upstream 100 bases:

>100_bases
TAGGTTGTTGCTAAAAATTAAATTTATTTTGGATTTCTTAAGTCGAATAAGAAATCCAAGTAGTAATTCATAAATATATG
GATTATATAGGGGGTTTAAA

Downstream 100 bases:

>100_bases
TTAAAAGGCACCTTTCATTTAAAAGGTGCCTTAGATCTTGTTAAGATGATAATTCTACATATATTCTCATTTGTTCTTCT
TTTATTTTTTTAGGTATCAT

Product: Na+/H+ antiporter NhaC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MNKGSHIRPNFLGLVPFLVFIIVYVGTGVALEMQGVEMAFYQMPPIIAMLLAVVTAFLLFKGSFIEKLNEFIEGCSQTDI
IFISFIFLISGAFSAVCKEIGSIETVANIGLKYIPANLLVAGIFLICLFLSTATGSFLGTVVAVTPIGFEIAGKSGIPLP
MVAGAVLGGGAFGDSMSLISDTTIIASRTQGVKIIDVFRNGVFFTFPASILSTVAFAILGSYVGGVGVNVELGEISYLKV
IPYLFVIIFAFLGVDVFLVLFLGILIAGGIGILNGDLTLLLMAQKINVGLLDLSEMLILVIFTGGISYMTISHGGFEWIL
TKLKYLAKCRRSAEFTITFLIVMVTGFLSNSGLAILVNETVTKGISEANSVCPKRCAALLSISSCALIGALPYGMHMISV
MNIAKGAISPLDIMPFLFYQVFLGIIIFSIAFLGLKNQFLNSTKP

Sequences:

>Translated_445_residues
MNKGSHIRPNFLGLVPFLVFIIVYVGTGVALEMQGVEMAFYQMPPIIAMLLAVVTAFLLFKGSFIEKLNEFIEGCSQTDI
IFISFIFLISGAFSAVCKEIGSIETVANIGLKYIPANLLVAGIFLICLFLSTATGSFLGTVVAVTPIGFEIAGKSGIPLP
MVAGAVLGGGAFGDSMSLISDTTIIASRTQGVKIIDVFRNGVFFTFPASILSTVAFAILGSYVGGVGVNVELGEISYLKV
IPYLFVIIFAFLGVDVFLVLFLGILIAGGIGILNGDLTLLLMAQKINVGLLDLSEMLILVIFTGGISYMTISHGGFEWIL
TKLKYLAKCRRSAEFTITFLIVMVTGFLSNSGLAILVNETVTKGISEANSVCPKRCAALLSISSCALIGALPYGMHMISV
MNIAKGAISPLDIMPFLFYQVFLGIIIFSIAFLGLKNQFLNSTKP
>Mature_445_residues
MNKGSHIRPNFLGLVPFLVFIIVYVGTGVALEMQGVEMAFYQMPPIIAMLLAVVTAFLLFKGSFIEKLNEFIEGCSQTDI
IFISFIFLISGAFSAVCKEIGSIETVANIGLKYIPANLLVAGIFLICLFLSTATGSFLGTVVAVTPIGFEIAGKSGIPLP
MVAGAVLGGGAFGDSMSLISDTTIIASRTQGVKIIDVFRNGVFFTFPASILSTVAFAILGSYVGGVGVNVELGEISYLKV
IPYLFVIIFAFLGVDVFLVLFLGILIAGGIGILNGDLTLLLMAQKINVGLLDLSEMLILVIFTGGISYMTISHGGFEWIL
TKLKYLAKCRRSAEFTITFLIVMVTGFLSNSGLAILVNETVTKGISEANSVCPKRCAALLSISSCALIGALPYGMHMISV
MNIAKGAISPLDIMPFLFYQVFLGIIIFSIAFLGLKNQFLNSTKP

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 47421; Mature: 47421

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKGSHIRPNFLGLVPFLVFIIVYVGTGVALEMQGVEMAFYQMPPIIAMLLAVVTAFLLF
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCHHHHHHHHHHHHHHHH
KGSFIEKLNEFIEGCSQTDIIFISFIFLISGAFSAVCKEIGSIETVANIGLKYIPANLLV
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHH
AGIFLICLFLSTATGSFLGTVVAVTPIGFEIAGKSGIPLPMVAGAVLGGGAFGDSMSLIS
HHHHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHH
DTTIIASRTQGVKIIDVFRNGVFFTFPASILSTVAFAILGSYVGGVGVNVELGEISYLKV
CCHHEEECCCCCEEEEEHHCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHH
IPYLFVIIFAFLGVDVFLVLFLGILIAGGIGILNGDLTLLLMAQKINVGLLDLSEMLILV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEHHHCCCCHHHHHHHHHHH
IFTGGISYMTISHGGFEWILTKLKYLAKCRRSAEFTITFLIVMVTGFLSNSGLAILVNET
HHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEHH
VTKGISEANSVCPKRCAALLSISSCALIGALPYGMHMISVMNIAKGAISPLDIMPFLFYQ
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VFLGIIIFSIAFLGLKNQFLNSTKP
HHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNKGSHIRPNFLGLVPFLVFIIVYVGTGVALEMQGVEMAFYQMPPIIAMLLAVVTAFLLF
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCHHHHHHHHHHHHHHHH
KGSFIEKLNEFIEGCSQTDIIFISFIFLISGAFSAVCKEIGSIETVANIGLKYIPANLLV
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHH
AGIFLICLFLSTATGSFLGTVVAVTPIGFEIAGKSGIPLPMVAGAVLGGGAFGDSMSLIS
HHHHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHH
DTTIIASRTQGVKIIDVFRNGVFFTFPASILSTVAFAILGSYVGGVGVNVELGEISYLKV
CCHHEEECCCCCEEEEEHHCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHH
IPYLFVIIFAFLGVDVFLVLFLGILIAGGIGILNGDLTLLLMAQKINVGLLDLSEMLILV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEHHHCCCCHHHHHHHHHHH
IFTGGISYMTISHGGFEWILTKLKYLAKCRRSAEFTITFLIVMVTGFLSNSGLAILVNET
HHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEHH
VTKGISEANSVCPKRCAALLSISSCALIGALPYGMHMISVMNIAKGAISPLDIMPFLFYQ
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VFLGIIIFSIAFLGLKNQFLNSTKP
HHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]