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The map label for this gene is glpF [H]

Identifier: 187918112

GI number: 187918112

Start: 245442

End: 246218

Strand: Direct

Name: glpF [H]

Synonym: BH0240

Alternate gene names: 187918112

Gene position: 245442-246218 (Clockwise)

Preceding gene: 187918109

Following gene: 187918113

Centisome position: 26.61

GC content: 35.78

Gene sequence:

>777_bases
ATGATTTATATACGATTTAAAGAATTTATTGCAGAATTTTTAGGCACATTTATTTTGCTAGCACTTGGAACAGGTTCTAT
AGCAATGACAACCCTATTCCCATCAAATCCTGCTGTAGCTGGAGAAATAATAAAGGGGGGCTATACAAATATAGTACTTG
GTTGGGGCCTAGGTGTAACATTTGGGGTTTATACAGCTGCAAAAATTAGTGGTGCACATTTAAATCCTGCCCTTAGCATT
GGACTTGCTAGTATTGGCAAATTTCCAACGTCAAAGCTTTTCCACTACATTTTAGCACAAATACTTGGGGCATTTTCTGG
AGCTCTAATGACACTAATTGTATTCTACCCTAAATGGATAGAAATAGATCCTACATTTGAAATTACCCAAGGCATCATGT
CAACTTTTCCCGCTGTCTCTGGTTTTTGGCCTGGATTTATTGACCAAATATTTGGAACATTTTTATTGATGTTTTTAGTT
CTAGTTGTTGGAAAGTTTGTCAAGGAAGAGACTCAAAACCCATTTTTCCCCTTCATTATTGGAGCAATAGTTCTAGCCAT
TGGAATAAGCTTTGGAGGAATGAATGGTTATGCTATTAACCCAGCAAGGGATTTGGGGCCAAGAATATTACTACTACTGG
CTGGTTTTAAAAATCACGGTTTTGATGACATGACTGTATTCATCATTCCTATATTAGGCCCAATAATTGGCGCTATTTTA
GGTGCTATAGTTTATGAATTTACCTTAGAAGACAAAAATAATCTTAAAGAATTTTAA

Upstream 100 bases:

>100_bases
AGATAGCTTATTACAGAAACATAATATTTTTAAAGGACGATAAATATTTTAGAAAGTTAATTTCAAATTAGATCTTATGT
AATATTAAGGAGGTTAAAGA

Downstream 100 bases:

>100_bases
TATAATCCAAATAACATTGATTATTTGGTTATTGAAATAATGCTAAGGAAGGGCAACTACTATGAAATATATTCTATCTC
TTGACCAAGGCACAACTAGT

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MIYIRFKEFIAEFLGTFILLALGTGSIAMTTLFPSNPAVAGEIIKGGYTNIVLGWGLGVTFGVYTAAKISGAHLNPALSI
GLASIGKFPTSKLFHYILAQILGAFSGALMTLIVFYPKWIEIDPTFEITQGIMSTFPAVSGFWPGFIDQIFGTFLLMFLV
LVVGKFVKEETQNPFFPFIIGAIVLAIGISFGGMNGYAINPARDLGPRILLLLAGFKNHGFDDMTVFIIPILGPIIGAIL
GAIVYEFTLEDKNNLKEF

Sequences:

>Translated_258_residues
MIYIRFKEFIAEFLGTFILLALGTGSIAMTTLFPSNPAVAGEIIKGGYTNIVLGWGLGVTFGVYTAAKISGAHLNPALSI
GLASIGKFPTSKLFHYILAQILGAFSGALMTLIVFYPKWIEIDPTFEITQGIMSTFPAVSGFWPGFIDQIFGTFLLMFLV
LVVGKFVKEETQNPFFPFIIGAIVLAIGISFGGMNGYAINPARDLGPRILLLLAGFKNHGFDDMTVFIIPILGPIIGAIL
GAIVYEFTLEDKNNLKEF
>Mature_258_residues
MIYIRFKEFIAEFLGTFILLALGTGSIAMTTLFPSNPAVAGEIIKGGYTNIVLGWGLGVTFGVYTAAKISGAHLNPALSI
GLASIGKFPTSKLFHYILAQILGAFSGALMTLIVFYPKWIEIDPTFEITQGIMSTFPAVSGFWPGFIDQIFGTFLLMFLV
LVVGKFVKEETQNPFFPFIIGAIVLAIGISFGGMNGYAINPARDLGPRILLLLAGFKNHGFDDMTVFIIPILGPIIGAIL
GAIVYEFTLEDKNNLKEF

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=260, Percent_Identity=35.7692307692308, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4502187, Length=253, Percent_Identity=33.596837944664, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI22538420, Length=250, Percent_Identity=32, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI157266307, Length=252, Percent_Identity=32.9365079365079, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI310133356, Length=212, Percent_Identity=31.1320754716981, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI310114181, Length=212, Percent_Identity=31.1320754716981, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI4502179, Length=246, Percent_Identity=28.8617886178862, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1790362, Length=252, Percent_Identity=35.3174603174603, Blast_Score=132, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71992966, Length=252, Percent_Identity=35.3174603174603, Blast_Score=139, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71994009, Length=253, Percent_Identity=33.9920948616601, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71992961, Length=251, Percent_Identity=31.4741035856574, Blast_Score=113, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI32564052, Length=264, Percent_Identity=31.4393939393939, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17533613, Length=264, Percent_Identity=31.4393939393939, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17531431, Length=250, Percent_Identity=32, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17531429, Length=250, Percent_Identity=32, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17544068, Length=212, Percent_Identity=38.2075471698113, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71993722, Length=246, Percent_Identity=28.4552845528455, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558372, Length=249, Percent_Identity=26.5060240963855, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=225, Percent_Identity=34.2222222222222, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6322985, Length=297, Percent_Identity=28.956228956229, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 27950; Mature: 27950

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00061 ADH_SHORT ; PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYIRFKEFIAEFLGTFILLALGTGSIAMTTLFPSNPAVAGEIIKGGYTNIVLGWGLGVT
CEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHCCCCCEEEEECCHHHH
FGVYTAAKISGAHLNPALSIGLASIGKFPTSKLFHYILAQILGAFSGALMTLIVFYPKWI
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
EIDPTFEITQGIMSTFPAVSGFWPGFIDQIFGTFLLMFLVLVVGKFVKEETQNPFFPFII
EECCCHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
GAIVLAIGISFGGMNGYAINPARDLGPRILLLLAGFKNHGFDDMTVFIIPILGPIIGAIL
HHHHHHHHHHCCCCCCEEECCHHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
GAIVYEFTLEDKNNLKEF
HHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIYIRFKEFIAEFLGTFILLALGTGSIAMTTLFPSNPAVAGEIIKGGYTNIVLGWGLGVT
CEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHCCCCCEEEEECCHHHH
FGVYTAAKISGAHLNPALSIGLASIGKFPTSKLFHYILAQILGAFSGALMTLIVFYPKWI
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
EIDPTFEITQGIMSTFPAVSGFWPGFIDQIFGTFLLMFLVLVVGKFVKEETQNPFFPFII
EECCCHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
GAIVLAIGISFGGMNGYAINPARDLGPRILLLLAGFKNHGFDDMTVFIIPILGPIIGAIL
HHHHHHHHHHCCCCCCEEECCHHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
GAIVYEFTLEDKNNLKEF
HHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]