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The map label for this gene is glpK

Identifier: 187918113

GI number: 187918113

Start: 246280

End: 247767

Strand: Direct

Name: glpK

Synonym: BH0241

Alternate gene names: 187918113

Gene position: 246280-247767 (Clockwise)

Preceding gene: 187918112

Following gene: 187918114

Centisome position: 26.7

GC content: 35.82

Gene sequence:

>1488_bases
ATGAAATATATTCTATCTCTTGACCAAGGCACAACTAGTTCAAGAGCGGTAATATTCGATAAGAATGCAAACATAAAAGG
ATTTGCAAAAAAGGAATTTAAACAAATTTATCCACATCCAAGTTGGGTTGAGCACGATCCAAACGAAATATGGGGCTCTC
TACTAGGAGTTATGGCAGAAGCTTTAGCAAACGCAAGAACTTTTCCCAATAATATTGAAGCAATTGGAATCACAAATCAA
AGAGAAACGACCATTATTTGGGATAGGAACACAGGACATCCAATTTATAACGCAATAGTCTGGCAAGACAGAAGAACCGC
ACAACTTTGTGATGAATTAAAATCAAAAGGAAAAGATAAAATTTTTTTACAAAAAACAGGTCTTGTATTAGATGCTTACT
TTAGCGGCACAAAAATAAAATGGATACTGGACAATGTTGCAGGAGCAAGAAAACGAGCAGAAAAAGGGGAACTATGTTTC
GGAACAATAGATACTTGGATAGTATGGAACCTTACTAAAGGAAAAATACATATTACAGATTATTCTAACGCATCAAGAAC
TCTACTTTTAAACATTAAATCACTTAAATGGGACTGTGATCTATTACAAATATTAGACATCCCAAAATCACTTTTACCTG
AACTTAAACAAAGTTCCGAGGTGTATGGCAAAACCGACGCTTCCGCACTGGGAACAGAAATCATTATTTCAGGAATTGCA
GGGGATCAATTTGCAGCAACATTTGGACAAGCATGTCTTCAAAAAGGAATGGCTAAAAATACCTATGGAACTGGCTGCTT
TGTCACTGTCAACATAGGAAAAAAACCCATCATAAATGAACAAAAAATTTTAACGTCAATTGCATGGGGAAGAAAAAATT
CAATAACTTATGTTTTTGAGGGAAGTGTTTTCATTGGGGGAGCTGTAATTCAATGGTTAAGAGACAATCTAGAACTATTT
AGAAAAAGCGGTGATGCAGAGGCAGTAGCAGCCTCAGTAGATAATAATGGGGGCATTTATTTCGTGCCAGCATTTGTAGG
ACTTGGAACACCTCATTGGGATCCTTATGCCAGAGGAATGATTATTGGACTTACAAGAAGCTCAACAAAAGAACATATTA
CAAGAGCGGCTCTTGAAAGCATTGCATTGCAAAGCTTTGATGTACTAACCGAAATGCAAAACTCTATTCAAGAATTTGAA
ATAAAGGAATTAAGAGTTGATGGGGGAGCTAGTAAAAATAATCTACTTATGCAATTCCAAGCTGATATTTTACAGTGCAA
TGTTGTAAGGCCCAAAATCACAGAAACGACCGCTCTTGGTTCTGCCTATCTAGCAGGACTTGCTGTTGGATATTGGGAAA
GTGCTGAAGAGATTACAAGTCTTTGGAAATCAGATAAAATATTTGAACCTTCAATGGAAAAAAGTAAAAGAGAAGATTTA
ATCTATAATTGGAATAAAGCTATTAAGAGGGCAAAAGCTTGGATTTAA

Upstream 100 bases:

>100_bases
TTTACCTTAGAAGACAAAAATAATCTTAAAGAATTTTAATATAATCCAAATAACATTGATTATTTGGTTATTGAAATAAT
GCTAAGGAAGGGCAACTACT

Downstream 100 bases:

>100_bases
TAACTTCCATTACTAATATTTCAAATATCCAAAGACTAATATTTCTTAAGCTATACACTTAATATATAAAATTAAAATTT
GGTAAATACATGGAGTATTC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MKYILSLDQGTTSSRAVIFDKNANIKGFAKKEFKQIYPHPSWVEHDPNEIWGSLLGVMAEALANARTFPNNIEAIGITNQ
RETTIIWDRNTGHPIYNAIVWQDRRTAQLCDELKSKGKDKIFLQKTGLVLDAYFSGTKIKWILDNVAGARKRAEKGELCF
GTIDTWIVWNLTKGKIHITDYSNASRTLLLNIKSLKWDCDLLQILDIPKSLLPELKQSSEVYGKTDASALGTEIIISGIA
GDQFAATFGQACLQKGMAKNTYGTGCFVTVNIGKKPIINEQKILTSIAWGRKNSITYVFEGSVFIGGAVIQWLRDNLELF
RKSGDAEAVAASVDNNGGIYFVPAFVGLGTPHWDPYARGMIIGLTRSSTKEHITRAALESIALQSFDVLTEMQNSIQEFE
IKELRVDGGASKNNLLMQFQADILQCNVVRPKITETTALGSAYLAGLAVGYWESAEEITSLWKSDKIFEPSMEKSKREDL
IYNWNKAIKRAKAWI

Sequences:

>Translated_495_residues
MKYILSLDQGTTSSRAVIFDKNANIKGFAKKEFKQIYPHPSWVEHDPNEIWGSLLGVMAEALANARTFPNNIEAIGITNQ
RETTIIWDRNTGHPIYNAIVWQDRRTAQLCDELKSKGKDKIFLQKTGLVLDAYFSGTKIKWILDNVAGARKRAEKGELCF
GTIDTWIVWNLTKGKIHITDYSNASRTLLLNIKSLKWDCDLLQILDIPKSLLPELKQSSEVYGKTDASALGTEIIISGIA
GDQFAATFGQACLQKGMAKNTYGTGCFVTVNIGKKPIINEQKILTSIAWGRKNSITYVFEGSVFIGGAVIQWLRDNLELF
RKSGDAEAVAASVDNNGGIYFVPAFVGLGTPHWDPYARGMIIGLTRSSTKEHITRAALESIALQSFDVLTEMQNSIQEFE
IKELRVDGGASKNNLLMQFQADILQCNVVRPKITETTALGSAYLAGLAVGYWESAEEITSLWKSDKIFEPSMEKSKREDL
IYNWNKAIKRAKAWI
>Mature_495_residues
MKYILSLDQGTTSSRAVIFDKNANIKGFAKKEFKQIYPHPSWVEHDPNEIWGSLLGVMAEALANARTFPNNIEAIGITNQ
RETTIIWDRNTGHPIYNAIVWQDRRTAQLCDELKSKGKDKIFLQKTGLVLDAYFSGTKIKWILDNVAGARKRAEKGELCF
GTIDTWIVWNLTKGKIHITDYSNASRTLLLNIKSLKWDCDLLQILDIPKSLLPELKQSSEVYGKTDASALGTEIIISGIA
GDQFAATFGQACLQKGMAKNTYGTGCFVTVNIGKKPIINEQKILTSIAWGRKNSITYVFEGSVFIGGAVIQWLRDNLELF
RKSGDAEAVAASVDNNGGIYFVPAFVGLGTPHWDPYARGMIIGLTRSSTKEHITRAALESIALQSFDVLTEMQNSIQEFE
IKELRVDGGASKNNLLMQFQADILQCNVVRPKITETTALGSAYLAGLAVGYWESAEEITSLWKSDKIFEPSMEKSKREDL
IYNWNKAIKRAKAWI

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI41393575, Length=500, Percent_Identity=44, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI189409120, Length=503, Percent_Identity=43.5387673956262, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI4504007, Length=503, Percent_Identity=43.5387673956262, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI42794763, Length=509, Percent_Identity=43.0255402750491, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI88196792, Length=508, Percent_Identity=33.4645669291339, Blast_Score=320, Evalue=2e-87,
Organism=Escherichia coli, GI1790361, Length=494, Percent_Identity=55.8704453441296, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1789138, Length=508, Percent_Identity=24.2125984251968, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787789, Length=518, Percent_Identity=25.4826254826255, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1790005, Length=470, Percent_Identity=23.6170212765957, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789987, Length=461, Percent_Identity=23.8611713665944, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535599, Length=505, Percent_Identity=40.1980198019802, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71986140, Length=517, Percent_Identity=26.8858800773694, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI71986147, Length=268, Percent_Identity=31.3432835820896, Blast_Score=132, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6321755, Length=548, Percent_Identity=35.948905109489, Blast_Score=305, Evalue=1e-83,
Organism=Drosophila melanogaster, GI17864214, Length=506, Percent_Identity=41.699604743083, Blast_Score=387, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24654573, Length=433, Percent_Identity=44.5727482678984, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655555, Length=512, Percent_Identity=38.0859375, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24655560, Length=512, Percent_Identity=38.0859375, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24655570, Length=512, Percent_Identity=38.0859375, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24655574, Length=512, Percent_Identity=38.0859375, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24655565, Length=512, Percent_Identity=38.0859375, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI19922058, Length=526, Percent_Identity=31.3688212927757, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI24652871, Length=493, Percent_Identity=31.6430020283976, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24656367, Length=521, Percent_Identity=29.1746641074856, Blast_Score=232, Evalue=4e-61,
Organism=Drosophila melanogaster, GI21357867, Length=529, Percent_Identity=27.9773156899811, Blast_Score=225, Evalue=5e-59,
Organism=Drosophila melanogaster, GI24656372, Length=353, Percent_Identity=30.028328611898, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI45552909, Length=291, Percent_Identity=28.1786941580756, Blast_Score=112, Evalue=6e-25,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_BORHD (B2RZV0)

Other databases:

- EMBL:   CP000048
- RefSeq:   YP_001883676.1
- ProteinModelPortal:   B2RZV0
- SMR:   B2RZV0
- EnsemblBacteria:   EBBORT00000006722
- GeneID:   6277081
- GenomeReviews:   CP000048_GR
- KEGG:   bhr:BH0241
- GeneTree:   EBGT00050000006825
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- ProtClustDB:   CLSK507458
- BioCyc:   BHER314723:BH0241-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55194; Mature: 55194

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 11-11 BINDING 15-15 BINDING 81-81 BINDING 133-133 BINDING 242-242 BINDING 264-264 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYILSLDQGTTSSRAVIFDKNANIKGFAKKEFKQIYPHPSWVEHDPNEIWGSLLGVMAE
CCEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
ALANARTFPNNIEAIGITNQRETTIIWDRNTGHPIYNAIVWQDRRTAQLCDELKSKGKDK
HHHCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCE
IFLQKTGLVLDAYFSGTKIKWILDNVAGARKRAEKGELCFGTIDTWIVWNLTKGKIHITD
EEEEECCEEEEEEECCCEEEEEHHHHHHHHHHCCCCCEEEEEECEEEEEEEECCEEEEEE
YSNASRTLLLNIKSLKWDCDLLQILDIPKSLLPELKQSSEVYGKTDASALGTEIIISGIA
CCCCCCEEEEEEECCCCCCHHHHHHCCCHHHHHHHHHCCHHCCCCCHHHHCCEEEEECCC
GDQFAATFGQACLQKGMAKNTYGTGCFVTVNIGKKPIINEQKILTSIAWGRKNSITYVFE
CCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEE
GSVFIGGAVIQWLRDNLELFRKSGDAEAVAASVDNNGGIYFVPAFVGLGTPHWDPYARGM
CCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEHHHCCCCCCCCHHHCCE
IIGLTRSSTKEHITRAALESIALQSFDVLTEMQNSIQEFEIKELRVDGGASKNNLLMQFQ
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEHEEEEECCCCCCCCEEEEEE
ADILQCNVVRPKITETTALGSAYLAGLAVGYWESAEEITSLWKSDKIFEPSMEKSKREDL
CCHHEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHH
IYNWNKAIKRAKAWI
EECHHHHHHHHHCCC
>Mature Secondary Structure
MKYILSLDQGTTSSRAVIFDKNANIKGFAKKEFKQIYPHPSWVEHDPNEIWGSLLGVMAE
CCEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
ALANARTFPNNIEAIGITNQRETTIIWDRNTGHPIYNAIVWQDRRTAQLCDELKSKGKDK
HHHCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCE
IFLQKTGLVLDAYFSGTKIKWILDNVAGARKRAEKGELCFGTIDTWIVWNLTKGKIHITD
EEEEECCEEEEEEECCCEEEEEHHHHHHHHHHCCCCCEEEEEECEEEEEEEECCEEEEEE
YSNASRTLLLNIKSLKWDCDLLQILDIPKSLLPELKQSSEVYGKTDASALGTEIIISGIA
CCCCCCEEEEEEECCCCCCHHHHHHCCCHHHHHHHHHCCHHCCCCCHHHHCCEEEEECCC
GDQFAATFGQACLQKGMAKNTYGTGCFVTVNIGKKPIINEQKILTSIAWGRKNSITYVFE
CCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEE
GSVFIGGAVIQWLRDNLELFRKSGDAEAVAASVDNNGGIYFVPAFVGLGTPHWDPYARGM
CCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEHHHCCCCCCCCHHHCCE
IIGLTRSSTKEHITRAALESIALQSFDVLTEMQNSIQEFEIKELRVDGGASKNNLLMQFQ
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEHEEEEECCCCCCCCEEEEEE
ADILQCNVVRPKITETTALGSAYLAGLAVGYWESAEEITSLWKSDKIFEPSMEKSKREDL
CCHHEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHH
IYNWNKAIKRAKAWI
EECHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA