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The map label for this gene is mutB [H]

Identifier: 187736463

GI number: 187736463

Start: 2406105

End: 2408249

Strand: Reverse

Name: mutB [H]

Synonym: Amuc_1983

Alternate gene names: 187736463

Gene position: 2408249-2406105 (Counterclockwise)

Preceding gene: 187736464

Following gene: 187736462

Centisome position: 90.4

GC content: 59.91

Gene sequence:

>2145_bases
ATGAGCAATATTCCTGATTTTGCATCCGCCTCCTATCCTGAAATCAAGGCTGGTGCCCCGTCCCCGGCTTCCGAAACGGA
AACCTGGATGACCAATGAACAAATTCCCGTGCCGCCCACCTACTCGGAAAGCGTGTACGACGACTGCCTGCACCTGGACT
TTACGGCCGGTGTGGCTCCCAACCTCCGCGGACCGTATGCCACCATGTATGTGGCGCGCCCCTGGACGGTGCGCCAGTAC
GCCGGCTTTTCCACGGCTGAGGAATCCAATGCCTTCTACCGCCGCAACCTGGCGGCGGGCCAGAAGGGCCTTTCCATTGC
CTTTGACCTGGCGACGCACCGCGGCTATGACTCCGATCACCCCCGCGTGGTGGGTGACGTGGGCAAGGCCGGCGTGGCCG
TGGACTCCATTCTGGACATGGAAATCCTCTTCAAGGGCATTCCCCTGGACAAAATGTCCGTTTCCATGACCATGAACGGC
GCCGTGTTGCCCGTGCTGGCCTTCTACATCGTCGCCGCCCAGGAACAGGGCTGCACGCTGGACCAGCTCTCCGGCACGAT
CCAGAATGACATTCTCAAGGAATACATGGTGCGCAACACCTACATTTACCCGCCTGATCCCTCCATGAGGATTATTGCGG
ACATCTTTGAGTTCACCTCCAAGTACATGCCCAAGTTTAACTCCATTTCCATCTCCGGCTACCACATGCAGGAGGCCGGC
GCTACGGCGGACCTGGAAATGGCCTACACCCTTGCGGACGGCCTAGAATATGTCCGCACCGGGATCAAGGCGGGCATTGA
CATTGACGCCTTCGCGCCGCGTCTTTCCTTCTTCTGGGCCCAGGGCAAGAATTACTTCATGGAAGTGGCCAAAATGCGCG
CCGCCCGCGTGCTGTGGGCCAAACTCATCAAGCAGTTCAATCCCAAGAACCCGAAGTCCCTGGCCTTGCGCACGCACTCC
CAGACTTCCGGCTGGTCCCTCACGGAACAGGACCCGTTCAACAACGTCACCCGCACCTGCATTGAGGCCCTAGCCGCTGC
CTGCGGCCATACGCAGTCCCTGCACACGAACTCCCTGGACGAAGCGATTGCCCTGCCGACGGACTTCTCCGCCCGCATCG
CCCGCAACACCCAGCTTCATCTTCAGGATGAAACCACCATCTGCAAGGTAATCGACCCGTGGGGCGGTTCCTACTATGTG
GAATACCTGACCAATGAGCTCATTCGCAAGGGCTGGGCCCACCTTCAGGAAGTGGAAAAACTCGGAGGCATGGCCAAGGC
CATTGAAACCGGGCTGCCCAAGATGCGCATTGAAGAAGCCGCAGCGCGCCGCCAGGCCGCCATTGACTCCGGAAAGGAAC
CCATCATCGGCGTTAACAAATACCGTCTGGAACAGGAAGCCCAGATCGATATTCTGGAAGTGGACAACACCACCGTCCGG
GAAGCCCAGATCGCCCGCCTGCAGAAACTGCGCGCGGAACGGGACTCCGCCGCGTGCGAAGCCGCGCTGGAAGCCCTGTC
CGAATGCGCCCGCACGGGGGAAGGCAATCTCCTTGACCTTGCCATCAAGGCGGCCAAGGCCCGCGCCTCCCTGGGTGAAA
TCTCCTCCGCCCTGGAAAAACACTTCGGGCGTCATAAAGCACCAATCAAACTCATTACCGGCGTGTACGCTCAATCCTAT
GGTCAAGATCCGCTGGTGGAGGAAGTCCGCAAGATGACGGATGACTTCGCCGAACGCACGGGGCGTCGTCCCCGCATCCT
TGTCGCCAAAATGGGCCAGGACGGCCATGACCGCGGCGCCAAGGTGGTCTCCTCCGCCTATGCCGACCTCGGTTTTGACG
TGGACGTGGGCCCGCTCTTCCAGACGCCGGAGGAAACTGCCAAAATGGCGATTGAAAACGACGTGCACATGATCGGCATG
AGCTCTCTGGCCGCCGGCCACAAGGTTCTCCTTCCCGCCCTGGTGGAGGAACTTGCCAAGCAGGGCCGTCCGGATATCCT
GGTCTTCTGCGGCGGCGTTATTCCCGCCCAGGACTATGACTTCCTGAAGGAACACGGAGCTGTGGCCATCTTCGGCCCGG
GCACCAACATTCCGGAAGCTTCCAGGGAAATCATGGAAGCTCTTAACGAACAGTACGCTGACTAA

Upstream 100 bases:

>100_bases
GCGTGGACATCTTCATCCACGTCAAGGCCAACTGCTACAACACCGTTGAAGCCATTCAAAACAAAATCGGCCTTTAATAA
CCACCAACCTATTCCACCCA

Downstream 100 bases:

>100_bases
ACGGAATTTCCTGTTTATCAGGTAAATACCTGTTGCGAGAGGCCGGATGAAAAATCCGGCCTCTTTTTTGTGAGCGGATT
GACTATCCGGTGAAAAAAAG

Product: methylmalonyl-CoA mutase

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MSNIPDFASASYPEIKAGAPSPASETETWMTNEQIPVPPTYSESVYDDCLHLDFTAGVAPNLRGPYATMYVARPWTVRQY
AGFSTAEESNAFYRRNLAAGQKGLSIAFDLATHRGYDSDHPRVVGDVGKAGVAVDSILDMEILFKGIPLDKMSVSMTMNG
AVLPVLAFYIVAAQEQGCTLDQLSGTIQNDILKEYMVRNTYIYPPDPSMRIIADIFEFTSKYMPKFNSISISGYHMQEAG
ATADLEMAYTLADGLEYVRTGIKAGIDIDAFAPRLSFFWAQGKNYFMEVAKMRAARVLWAKLIKQFNPKNPKSLALRTHS
QTSGWSLTEQDPFNNVTRTCIEALAAACGHTQSLHTNSLDEAIALPTDFSARIARNTQLHLQDETTICKVIDPWGGSYYV
EYLTNELIRKGWAHLQEVEKLGGMAKAIETGLPKMRIEEAAARRQAAIDSGKEPIIGVNKYRLEQEAQIDILEVDNTTVR
EAQIARLQKLRAERDSAACEAALEALSECARTGEGNLLDLAIKAAKARASLGEISSALEKHFGRHKAPIKLITGVYAQSY
GQDPLVEEVRKMTDDFAERTGRRPRILVAKMGQDGHDRGAKVVSSAYADLGFDVDVGPLFQTPEETAKMAIENDVHMIGM
SSLAAGHKVLLPALVEELAKQGRPDILVFCGGVIPAQDYDFLKEHGAVAIFGPGTNIPEASREIMEALNEQYAD

Sequences:

>Translated_714_residues
MSNIPDFASASYPEIKAGAPSPASETETWMTNEQIPVPPTYSESVYDDCLHLDFTAGVAPNLRGPYATMYVARPWTVRQY
AGFSTAEESNAFYRRNLAAGQKGLSIAFDLATHRGYDSDHPRVVGDVGKAGVAVDSILDMEILFKGIPLDKMSVSMTMNG
AVLPVLAFYIVAAQEQGCTLDQLSGTIQNDILKEYMVRNTYIYPPDPSMRIIADIFEFTSKYMPKFNSISISGYHMQEAG
ATADLEMAYTLADGLEYVRTGIKAGIDIDAFAPRLSFFWAQGKNYFMEVAKMRAARVLWAKLIKQFNPKNPKSLALRTHS
QTSGWSLTEQDPFNNVTRTCIEALAAACGHTQSLHTNSLDEAIALPTDFSARIARNTQLHLQDETTICKVIDPWGGSYYV
EYLTNELIRKGWAHLQEVEKLGGMAKAIETGLPKMRIEEAAARRQAAIDSGKEPIIGVNKYRLEQEAQIDILEVDNTTVR
EAQIARLQKLRAERDSAACEAALEALSECARTGEGNLLDLAIKAAKARASLGEISSALEKHFGRHKAPIKLITGVYAQSY
GQDPLVEEVRKMTDDFAERTGRRPRILVAKMGQDGHDRGAKVVSSAYADLGFDVDVGPLFQTPEETAKMAIENDVHMIGM
SSLAAGHKVLLPALVEELAKQGRPDILVFCGGVIPAQDYDFLKEHGAVAIFGPGTNIPEASREIMEALNEQYAD
>Mature_713_residues
SNIPDFASASYPEIKAGAPSPASETETWMTNEQIPVPPTYSESVYDDCLHLDFTAGVAPNLRGPYATMYVARPWTVRQYA
GFSTAEESNAFYRRNLAAGQKGLSIAFDLATHRGYDSDHPRVVGDVGKAGVAVDSILDMEILFKGIPLDKMSVSMTMNGA
VLPVLAFYIVAAQEQGCTLDQLSGTIQNDILKEYMVRNTYIYPPDPSMRIIADIFEFTSKYMPKFNSISISGYHMQEAGA
TADLEMAYTLADGLEYVRTGIKAGIDIDAFAPRLSFFWAQGKNYFMEVAKMRAARVLWAKLIKQFNPKNPKSLALRTHSQ
TSGWSLTEQDPFNNVTRTCIEALAAACGHTQSLHTNSLDEAIALPTDFSARIARNTQLHLQDETTICKVIDPWGGSYYVE
YLTNELIRKGWAHLQEVEKLGGMAKAIETGLPKMRIEEAAARRQAAIDSGKEPIIGVNKYRLEQEAQIDILEVDNTTVRE
AQIARLQKLRAERDSAACEAALEALSECARTGEGNLLDLAIKAAKARASLGEISSALEKHFGRHKAPIKLITGVYAQSYG
QDPLVEEVRKMTDDFAERTGRRPRILVAKMGQDGHDRGAKVVSSAYADLGFDVDVGPLFQTPEETAKMAIENDVHMIGMS
SLAAGHKVLLPALVEELAKQGRPDILVFCGGVIPAQDYDFLKEHGAVAIFGPGTNIPEASREIMEALNEQYAD

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=708, Percent_Identity=61.5819209039548, Blast_Score=919, Evalue=0.0,
Organism=Escherichia coli, GI1789284, Length=686, Percent_Identity=62.2448979591837, Blast_Score=874, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144500, Length=687, Percent_Identity=62.882096069869, Blast_Score=909, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 78416; Mature: 78285

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIPDFASASYPEIKAGAPSPASETETWMTNEQIPVPPTYSESVYDDCLHLDFTAGVAP
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHEEEECCCCCC
NLRGPYATMYVARPWTVRQYAGFSTAEESNAFYRRNLAAGQKGLSIAFDLATHRGYDSDH
CCCCCEEEEEEECCCCHHHHCCCCCCHHCCHHHHHHHHCCCCCCEEEEEEHHCCCCCCCC
PRVVGDVGKAGVAVDSILDMEILFKGIPLDKMSVSMTMNGAVLPVLAFYIVAAQEQGCTL
CEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCH
DQLSGTIQNDILKEYMVRNTYIYPPDPSMRIIADIFEFTSKYMPKFNSISISGYHMQEAG
HHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEECEEECCCC
ATADLEMAYTLADGLEYVRTGIKAGIDIDAFAPRLSFFWAQGKNYFMEVAKMRAARVLWA
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KLIKQFNPKNPKSLALRTHSQTSGWSLTEQDPFNNVTRTCIEALAAACGHTQSLHTNSLD
HHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCH
EAIALPTDFSARIARNTQLHLQDETTICKVIDPWGGSYYVEYLTNELIRKGWAHLQEVEK
HHHCCCCCCHHHHHCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LGGMAKAIETGLPKMRIEEAAARRQAAIDSGKEPIIGVNKYRLEQEAQIDILEVDNTTVR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEECCHHHHCCCCCCEEEEECCCCHHH
EAQIARLQKLRAERDSAACEAALEALSECARTGEGNLLDLAIKAAKARASLGEISSALEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
HFGRHKAPIKLITGVYAQSYGQDPLVEEVRKMTDDFAERTGRRPRILVAKMGQDGHDRGA
HHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHH
KVVSSAYADLGFDVDVGPLFQTPEETAKMAIENDVHMIGMSSLAAGHKVLLPALVEELAK
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHHHHHHHH
QGRPDILVFCGGVIPAQDYDFLKEHGAVAIFGPGTNIPEASREIMEALNEQYAD
CCCCCEEEEECCCCCCCCHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNIPDFASASYPEIKAGAPSPASETETWMTNEQIPVPPTYSESVYDDCLHLDFTAGVAP
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHEEEECCCCCC
NLRGPYATMYVARPWTVRQYAGFSTAEESNAFYRRNLAAGQKGLSIAFDLATHRGYDSDH
CCCCCEEEEEEECCCCHHHHCCCCCCHHCCHHHHHHHHCCCCCCEEEEEEHHCCCCCCCC
PRVVGDVGKAGVAVDSILDMEILFKGIPLDKMSVSMTMNGAVLPVLAFYIVAAQEQGCTL
CEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCH
DQLSGTIQNDILKEYMVRNTYIYPPDPSMRIIADIFEFTSKYMPKFNSISISGYHMQEAG
HHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEECEEECCCC
ATADLEMAYTLADGLEYVRTGIKAGIDIDAFAPRLSFFWAQGKNYFMEVAKMRAARVLWA
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KLIKQFNPKNPKSLALRTHSQTSGWSLTEQDPFNNVTRTCIEALAAACGHTQSLHTNSLD
HHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCH
EAIALPTDFSARIARNTQLHLQDETTICKVIDPWGGSYYVEYLTNELIRKGWAHLQEVEK
HHHCCCCCCHHHHHCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LGGMAKAIETGLPKMRIEEAAARRQAAIDSGKEPIIGVNKYRLEQEAQIDILEVDNTTVR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEECCHHHHCCCCCCEEEEECCCCHHH
EAQIARLQKLRAERDSAACEAALEALSECARTGEGNLLDLAIKAAKARASLGEISSALEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
HFGRHKAPIKLITGVYAQSYGQDPLVEEVRKMTDDFAERTGRRPRILVAKMGQDGHDRGA
HHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHH
KVVSSAYADLGFDVDVGPLFQTPEETAKMAIENDVHMIGMSSLAAGHKVLLPALVEELAK
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHHHHHHHH
QGRPDILVFCGGVIPAQDYDFLKEHGAVAIFGPGTNIPEASREIMEALNEQYAD
CCCCCEEEEECCCCCCCCHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8566763; 12949112 [H]