Click here to switch to the map view.

The map label for this gene is fadH [C]

Identifier: 187736433

GI number: 187736433

Start: 2368957

End: 2370060

Strand: Direct

Name: fadH [C]

Synonym: Amuc_1951

Alternate gene names: 187736433

Gene position: 2368957-2370060 (Clockwise)

Preceding gene: 187736432

Following gene: 187736439

Centisome position: 88.92

GC content: 57.88

Gene sequence:

>1104_bases
ATGGAAATCCTGTTCCAGCCCTTCCACTCACGCAAGCTCAGCACTCCCACCCGCATCGTCCTTCCGGCCATGACCCGGGG
ATTTTCTCCCAACGGGGTTCCCACGGACCAGGTAGCCGCTTATTACAAAAGGCGGGCCAGACATGAAGTAGGGCTCATCA
TCACGGAGGGCACTTTCATTGACGAGCCCAGCGCCTCGCCTTCCTCCAACTATCCCAATTTCTTCGGGGGCGCTTCCCTG
CGGGGATGGAAAAAGGTGCTGGAAGCCGTCCACACCACGGATTGTAAAATAGCCCCGCAACTCTGGCATGTGGGTATGGC
CCGGCCCTTCAAGGGAGAAAACCTGCCCAATCCGGAGTTGCCGCCCATCGGCCCTTCCGGAATTGACGTAAACACGCTGG
AACAGACGGCGGAACCGATGAGCATCGCTAAAATTGAGGAGGTTATCAATGCTTTTGCGAGGGCGGCTGCGGACGCCAAG
CGGCTGGGTTTTGACGGTGTGGAACTGCATGGAGCGCACGGCTACCTGATTGACCAGTTTTTCTGGAAGGAGACCAACAG
GCGCACGGACGAATACGGAGGCGACCTGGTAGGCCGGACCCGTTTTGCCAGCCGCATCATCCATGCCGTCCGCAAGGCAG
TGGGCAGCCAGTTCCCCATCATCTTCCGCTTCTCCCAATGGAAAACAGTCCATTATAACGCCAAGCTGGCGTATACCCCC
ATGGAACTGGAAGCTTTTCTGGCTCCGCTGACGGATGCGGGCGTGGATATCTTTGACTGTTCCACGCGCCGGTTTTGGGA
ACCGGAGTTTGAAGGTACCCGCCTCAATCTGGCCGGCTGGACCAGGAAGCTTACCGGAAAGCCCACGATCTCCGTCGGCT
CCGTGGGGCTTAAGGGAGATTTTACGAATGCCTTTGACGGGGGGCCGGAAGCGGAGGCCGCCAGTGTGGAACCTCTGGTG
GAACGGCTGAAAGCAGGGGAATTTGACCTGATTGCCGTAGGCCGCGCCCTGCTGGCGGATGCGGAATGGGCGGAAAAAAT
ACGCCATGCGCGGGAAAAAGATATCCACCTGTTTACGAAGGAAGACCTGAAAACGCTGAAATAA

Upstream 100 bases:

>100_bases
GTGTCTCACTCCCGTGCGGTTCCGGTTCCCATGCCCGGCCGCTTCAGAGATAACGTTTTTCACCTTTATGAATGCACAGG
ACGAACTCAAGATCAGGGAC

Downstream 100 bases:

>100_bases
CGGATTTGCCTGACCGCAAGGATGATGCGGGAACTCACTCCGCAAAGCCGGGAGGGGCAAAAACGCCAGGCCGCTCCAAA
ACGGAGGAGCCGTCTTCTTT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MEILFQPFHSRKLSTPTRIVLPAMTRGFSPNGVPTDQVAAYYKRRARHEVGLIITEGTFIDEPSASPSSNYPNFFGGASL
RGWKKVLEAVHTTDCKIAPQLWHVGMARPFKGENLPNPELPPIGPSGIDVNTLEQTAEPMSIAKIEEVINAFARAAADAK
RLGFDGVELHGAHGYLIDQFFWKETNRRTDEYGGDLVGRTRFASRIIHAVRKAVGSQFPIIFRFSQWKTVHYNAKLAYTP
MELEAFLAPLTDAGVDIFDCSTRRFWEPEFEGTRLNLAGWTRKLTGKPTISVGSVGLKGDFTNAFDGGPEAEAASVEPLV
ERLKAGEFDLIAVGRALLADAEWAEKIRHAREKDIHLFTKEDLKTLK

Sequences:

>Translated_367_residues
MEILFQPFHSRKLSTPTRIVLPAMTRGFSPNGVPTDQVAAYYKRRARHEVGLIITEGTFIDEPSASPSSNYPNFFGGASL
RGWKKVLEAVHTTDCKIAPQLWHVGMARPFKGENLPNPELPPIGPSGIDVNTLEQTAEPMSIAKIEEVINAFARAAADAK
RLGFDGVELHGAHGYLIDQFFWKETNRRTDEYGGDLVGRTRFASRIIHAVRKAVGSQFPIIFRFSQWKTVHYNAKLAYTP
MELEAFLAPLTDAGVDIFDCSTRRFWEPEFEGTRLNLAGWTRKLTGKPTISVGSVGLKGDFTNAFDGGPEAEAASVEPLV
ERLKAGEFDLIAVGRALLADAEWAEKIRHAREKDIHLFTKEDLKTLK
>Mature_367_residues
MEILFQPFHSRKLSTPTRIVLPAMTRGFSPNGVPTDQVAAYYKRRARHEVGLIITEGTFIDEPSASPSSNYPNFFGGASL
RGWKKVLEAVHTTDCKIAPQLWHVGMARPFKGENLPNPELPPIGPSGIDVNTLEQTAEPMSIAKIEEVINAFARAAADAK
RLGFDGVELHGAHGYLIDQFFWKETNRRTDEYGGDLVGRTRFASRIIHAVRKAVGSQFPIIFRFSQWKTVHYNAKLAYTP
MELEAFLAPLTDAGVDIFDCSTRRFWEPEFEGTRLNLAGWTRKLTGKPTISVGSVGLKGDFTNAFDGGPEAEAASVEPLV
ERLKAGEFDLIAVGRALLADAEWAEKIRHAREKDIHLFTKEDLKTLK

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=364, Percent_Identity=29.3956043956044, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787939, Length=224, Percent_Identity=32.1428571428571, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17559804, Length=221, Percent_Identity=30.7692307692308, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=197, Percent_Identity=31.4720812182741, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI72001454, Length=212, Percent_Identity=30.6603773584906, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=200, Percent_Identity=31.5, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17559802, Length=338, Percent_Identity=27.810650887574, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17565138, Length=335, Percent_Identity=26.5671641791045, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17566914, Length=199, Percent_Identity=30.1507537688442, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=227, Percent_Identity=31.2775330396476, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321973, Length=226, Percent_Identity=32.7433628318584, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 40825; Mature: 40825

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEILFQPFHSRKLSTPTRIVLPAMTRGFSPNGVPTDQVAAYYKRRARHEVGLIITEGTFI
CCCEECCCCCCCCCCCCEEEEHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCEEC
DEPSASPSSNYPNFFGGASLRGWKKVLEAVHTTDCKIAPQLWHVGMARPFKGENLPNPEL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECHHHHHCCCCCCCCCCCCCCCCC
PPIGPSGIDVNTLEQTAEPMSIAKIEEVINAFARAAADAKRLGFDGVELHGAHGYLIDQF
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHH
FWKETNRRTDEYGGDLVGRTRFASRIIHAVRKAVGSQFPIIFRFSQWKTVHYNAKLAYTP
HHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEECCEEEECC
MELEAFLAPLTDAGVDIFDCSTRRFWEPEFEGTRLNLAGWTRKLTGKPTISVGSVGLKGD
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEEECCCEEECCCCCCEEECCCCCCCC
FTNAFDGGPEAEAASVEPLVERLKAGEFDLIAVGRALLADAEWAEKIRHAREKDIHLFTK
CCCCCCCCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHCHHHHHHHHHHHHCCEEEEEH
EDLKTLK
HHHHHCC
>Mature Secondary Structure
MEILFQPFHSRKLSTPTRIVLPAMTRGFSPNGVPTDQVAAYYKRRARHEVGLIITEGTFI
CCCEECCCCCCCCCCCCEEEEHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCEEC
DEPSASPSSNYPNFFGGASLRGWKKVLEAVHTTDCKIAPQLWHVGMARPFKGENLPNPEL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECHHHHHCCCCCCCCCCCCCCCCC
PPIGPSGIDVNTLEQTAEPMSIAKIEEVINAFARAAADAKRLGFDGVELHGAHGYLIDQF
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHH
FWKETNRRTDEYGGDLVGRTRFASRIIHAVRKAVGSQFPIIFRFSQWKTVHYNAKLAYTP
HHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEECCEEEECC
MELEAFLAPLTDAGVDIFDCSTRRFWEPEFEGTRLNLAGWTRKLTGKPTISVGSVGLKGD
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEEECCCEEECCCCCCEEECCCCCCCC
FTNAFDGGPEAEAASVEPLVERLKAGEFDLIAVGRALLADAEWAEKIRHAREKDIHLFTK
CCCCCCCCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHCHHHHHHHHHHHHCCEEEEEH
EDLKTLK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]