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The map label for this gene is garR [C]

Identifier: 187736426

GI number: 187736426

Start: 2361482

End: 2362348

Strand: Direct

Name: garR [C]

Synonym: Amuc_1944

Alternate gene names: 187736426

Gene position: 2361482-2362348 (Clockwise)

Preceding gene: 187736425

Following gene: 187736427

Centisome position: 88.64

GC content: 56.52

Gene sequence:

>867_bases
ATGGAATTCTTCCCTTTCCCACTAAATAAAGCCATGTATCACGTTTCCATTTTAGGACTGGGCCTCATTGGCTCACGGAT
TGCACGCCATATGGCCGGAATGGGCGACAAGGTCACCGTCTGGAACAGAACTCCGCGGGAAGATTTTCCGGAAGCGGTTC
CTACCCCGGCGGATGCGGCACGCGCTTCCAAAGTTATCCAGCTCTACCTGAAAGACAGGAATGCCTGTCTGGAAGTGTTT
GAACAAATGAGAGGGGCTCTGACACCGGAGCATATCATTCTGAACCATTCAACCATTGACCTGGCGACCGTCGGCAAACT
CTCCCTGATGAGTTCCGCCATCGGCTGCACGTATGTGGATTGCCCGTTCACCGGCTCCCGTTTGCCCGCGGAAAAAGGGG
AACTGGTTTACTACGTTGGCACGGATTCCACCACACTGGACCGCATACGCCCCGTGCTGGAGCACAGTTCCCGGGACATC
CTGCACCTGGGCGGCATCGGAGCCGGCACCGTCGTCAAACTGGTCACCAACATGGTCTCCGCCACCATGGTGCAGAGTTT
GGGCGAAGCCCTGGCCATCTCCCAGGCGTACGGCATTTCCCCGGAAATGCTGGGCCAGGCCATTTCCCGGAACGTCATCG
CCTCCCCTCTGGCTGCATTTAAACTGCCCCTGATGGCAAAGCGTGACTTTTCCACCCATTTCTCCCTGGACAACATGAGG
AAAGACAGTATTTACGCTCAGGAACTTGCCGCAGAAAAAGGAATCCACCCGCCTGTTGCCGCCCTGGTTTCCTCCATCAT
GGGGAGGCTGTGCCGGGAAGGTCGCGCGGAAGAGGATTTCAGCTGTCTGGCGGAACAATTCGACTAA

Upstream 100 bases:

>100_bases
CACAACCTCCGGCAGAAGCCGCAACTGCCGCAACGGAAACGCCCAAGCCGGAAAAGCAGGAACCTCCGCGCAGGAAAAGC
CGTTTCCGAATCGGTTCCTG

Downstream 100 bases:

>100_bases
CTTTTTCCGTTGATGAAATTCAGGGGAACATGCGTCTTTCTGTTGACGGCTCTTCTTCTGCTCCCTGCCGGCCGGGGACA
GGAACGAAAGGAGCCTGGCA

Product: 6-phosphogluconate dehydrogenase NAD-binding

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MEFFPFPLNKAMYHVSILGLGLIGSRIARHMAGMGDKVTVWNRTPREDFPEAVPTPADAARASKVIQLYLKDRNACLEVF
EQMRGALTPEHIILNHSTIDLATVGKLSLMSSAIGCTYVDCPFTGSRLPAEKGELVYYVGTDSTTLDRIRPVLEHSSRDI
LHLGGIGAGTVVKLVTNMVSATMVQSLGEALAISQAYGISPEMLGQAISRNVIASPLAAFKLPLMAKRDFSTHFSLDNMR
KDSIYAQELAAEKGIHPPVAALVSSIMGRLCREGRAEEDFSCLAEQFD

Sequences:

>Translated_288_residues
MEFFPFPLNKAMYHVSILGLGLIGSRIARHMAGMGDKVTVWNRTPREDFPEAVPTPADAARASKVIQLYLKDRNACLEVF
EQMRGALTPEHIILNHSTIDLATVGKLSLMSSAIGCTYVDCPFTGSRLPAEKGELVYYVGTDSTTLDRIRPVLEHSSRDI
LHLGGIGAGTVVKLVTNMVSATMVQSLGEALAISQAYGISPEMLGQAISRNVIASPLAAFKLPLMAKRDFSTHFSLDNMR
KDSIYAQELAAEKGIHPPVAALVSSIMGRLCREGRAEEDFSCLAEQFD
>Mature_288_residues
MEFFPFPLNKAMYHVSILGLGLIGSRIARHMAGMGDKVTVWNRTPREDFPEAVPTPADAARASKVIQLYLKDRNACLEVF
EQMRGALTPEHIILNHSTIDLATVGKLSLMSSAIGCTYVDCPFTGSRLPAEKGELVYYVGTDSTTLDRIRPVLEHSSRDI
LHLGGIGAGTVVKLVTNMVSATMVQSLGEALAISQAYGISPEMLGQAISRNVIASPLAAFKLPLMAKRDFSTHFSLDNMR
KDSIYAQELAAEKGIHPPVAALVSSIMGRLCREGRAEEDFSCLAEQFD

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=278, Percent_Identity=24.1007194244604, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI23308751, Length=284, Percent_Identity=22.887323943662, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI145693186, Length=284, Percent_Identity=26.056338028169, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786719, Length=282, Percent_Identity=23.758865248227, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1790315, Length=285, Percent_Identity=22.8070175438596, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17557316, Length=293, Percent_Identity=23.8907849829352, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28574115, Length=278, Percent_Identity=25.5395683453237, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24655230, Length=292, Percent_Identity=23.972602739726, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19922568, Length=292, Percent_Identity=23.972602739726, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31342; Mature: 31342

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFFPFPLNKAMYHVSILGLGLIGSRIARHMAGMGDKVTVWNRTPREDFPEAVPTPADAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCCCCCCCHHHH
RASKVIQLYLKDRNACLEVFEQMRGALTPEHIILNHSTIDLATVGKLSLMSSAIGCTYVD
HHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEECCEECHHHHHHHHHHHHHCCCEEEE
CPFTGSRLPAEKGELVYYVGTDSTTLDRIRPVLEHSSRDILHLGGIGAGTVVKLVTNMVS
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHH
ATMVQSLGEALAISQAYGISPEMLGQAISRNVIASPLAAFKLPLMAKRDFSTHFSLDNMR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHCCHHHHH
KDSIYAQELAAEKGIHPPVAALVSSIMGRLCREGRAEEDFSCLAEQFD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MEFFPFPLNKAMYHVSILGLGLIGSRIARHMAGMGDKVTVWNRTPREDFPEAVPTPADAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHCCCCCCCCHHHH
RASKVIQLYLKDRNACLEVFEQMRGALTPEHIILNHSTIDLATVGKLSLMSSAIGCTYVD
HHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEEEECCEECHHHHHHHHHHHHHCCCEEEE
CPFTGSRLPAEKGELVYYVGTDSTTLDRIRPVLEHSSRDILHLGGIGAGTVVKLVTNMVS
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHH
ATMVQSLGEALAISQAYGISPEMLGQAISRNVIASPLAAFKLPLMAKRDFSTHFSLDNMR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHCCHHHHH
KDSIYAQELAAEKGIHPPVAALVSSIMGRLCREGRAEEDFSCLAEQFD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA