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The map label for this gene is rlmI [H]

Identifier: 187736308

GI number: 187736308

Start: 2210941

End: 2212125

Strand: Reverse

Name: rlmI [H]

Synonym: Amuc_1821

Alternate gene names: 187736308

Gene position: 2212125-2210941 (Counterclockwise)

Preceding gene: 187736312

Following gene: 187736307

Centisome position: 83.03

GC content: 57.89

Gene sequence:

>1185_bases
ATGGCAGGTTTGATTATTTCTCCAAGAGCACGCATTTTTCAAGGGCACGACTGGGTTTACGGCACGGAAGTGCGCAAAAT
TTTCGGCAATCCGCAGCCGGGGGACGTCGTGGCCCTGAAGGATTTCAAGGACCGCTTTCTGGGTTCCGCCATGTTCAATC
CCCATTCCCAGATCGTCGCCAGGCGCTTTTCCCGCCGCAAACAGGAACTGAACGGAGATTTCTTTTCCAGGCGCATCAGC
CAGGCAGTAGAACTGCGCCGCCGCCGGCTTCCGGAAGAAACTCTCACCCGGCTCGTTTGGAGCGAATCCGACGGGCTTCC
CGGCCTCATTGTGGACCGTTACGCGGATTATCTGGTCGTCCAGACGCTGACGATCGCCATGGAATGCCGCCTCCCCATCA
TCCTGAATGTTTTGGAAGACCTTCTTTCTCCCCGCGGGATTATTGTCAGGAATGATTCACCCATGCTGGCGGCAGAAGGT
ATTTCCCCCTCCGTCCGGGTGGCACGAGGACAGCAACCGGAACCTTTTGCCGCACGCAGCGGCAGCGTGCAATTCATGAT
TGACCTTCAGACGGGACAAAAAACCGGCCTGTATCTGGACCAGCTTGACAATTATGCCGCCGTGGCTCGCTTCGCCCGCG
GACGCCGCGTGCTGGACTGCTTCTGCAACCAGGGCGGTTTCGCCCTGGCCTGCGCCCTTGCCGGTGCCTCGGAGGTAACG
GCCGTGGACGTTTCCCAGGATGCTATGGACGCCGTAGCGCGGAACGCCCGCCTGAACGGAGTCTCCGTGCAGTGCGTCAC
GGATAACGCGTTTGACTTCCTGAAAAAGGAAGCGGCCCTTGTCCGGGACGGAGGAGAACACAAATGGGATTTAATTATCC
TGGATCCGCCCTCTTTTACCAGAAACAAAAAATCCGTGCATGACGCCATGCGCGGATATAAGGAAATCCACCTCCGCGCC
ATGAAGCTTCTGGCCCCGGGAGGCATCCTTTCCACCTTCTGCTGTTCCCACCACGCCGGAGCGGACCTGTTCCGGGAGAG
CGTGCTTGACGCCGCCATTGATGCTCCGGCCACCCTGCGTCTGATGCAGCAACACGGCCAAAGAGCGGATCATCCGGTTT
TATTGAATATTCCGGAAACGGAATACCTGAAGGGGTTCACGTATGAACTGCTTCCCGGAAGATGA

Upstream 100 bases:

>100_bases
TCCCAGGGAACGGAGGCCTCCACCCTTCCCACGGCCTTTCTGGGAGCCGCCCGCATTACCCTTGAACTTTCCACCCCGAT
CATGTATTGCCTTTCCTGAC

Downstream 100 bases:

>100_bases
TTTCATGACAATAACAATAACCATTCCTCCGGTCCCGATATTGTCAAAATGAAGTAAAAATACGATGCTATTTCCATTGA
ACAAAAACATCGTTCCAATG

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAGLIISPRARIFQGHDWVYGTEVRKIFGNPQPGDVVALKDFKDRFLGSAMFNPHSQIVARRFSRRKQELNGDFFSRRIS
QAVELRRRRLPEETLTRLVWSESDGLPGLIVDRYADYLVVQTLTIAMECRLPIILNVLEDLLSPRGIIVRNDSPMLAAEG
ISPSVRVARGQQPEPFAARSGSVQFMIDLQTGQKTGLYLDQLDNYAAVARFARGRRVLDCFCNQGGFALACALAGASEVT
AVDVSQDAMDAVARNARLNGVSVQCVTDNAFDFLKKEAALVRDGGEHKWDLIILDPPSFTRNKKSVHDAMRGYKEIHLRA
MKLLAPGGILSTFCCSHHAGADLFRESVLDAAIDAPATLRLMQQHGQRADHPVLLNIPETEYLKGFTYELLPGR

Sequences:

>Translated_394_residues
MAGLIISPRARIFQGHDWVYGTEVRKIFGNPQPGDVVALKDFKDRFLGSAMFNPHSQIVARRFSRRKQELNGDFFSRRIS
QAVELRRRRLPEETLTRLVWSESDGLPGLIVDRYADYLVVQTLTIAMECRLPIILNVLEDLLSPRGIIVRNDSPMLAAEG
ISPSVRVARGQQPEPFAARSGSVQFMIDLQTGQKTGLYLDQLDNYAAVARFARGRRVLDCFCNQGGFALACALAGASEVT
AVDVSQDAMDAVARNARLNGVSVQCVTDNAFDFLKKEAALVRDGGEHKWDLIILDPPSFTRNKKSVHDAMRGYKEIHLRA
MKLLAPGGILSTFCCSHHAGADLFRESVLDAAIDAPATLRLMQQHGQRADHPVLLNIPETEYLKGFTYELLPGR
>Mature_393_residues
AGLIISPRARIFQGHDWVYGTEVRKIFGNPQPGDVVALKDFKDRFLGSAMFNPHSQIVARRFSRRKQELNGDFFSRRISQ
AVELRRRRLPEETLTRLVWSESDGLPGLIVDRYADYLVVQTLTIAMECRLPIILNVLEDLLSPRGIIVRNDSPMLAAEGI
SPSVRVARGQQPEPFAARSGSVQFMIDLQTGQKTGLYLDQLDNYAAVARFARGRRVLDCFCNQGGFALACALAGASEVTA
VDVSQDAMDAVARNARLNGVSVQCVTDNAFDFLKKEAALVRDGGEHKWDLIILDPPSFTRNKKSVHDAMRGYKEIHLRAM
KLLAPGGILSTFCCSHHAGADLFRESVLDAAIDAPATLRLMQQHGQRADHPVLLNIPETEYLKGFTYELLPGR

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=378, Percent_Identity=37.037037037037, Blast_Score=247, Evalue=8e-67,
Organism=Escherichia coli, GI1787181, Length=229, Percent_Identity=27.5109170305677, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43791; Mature: 43660

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGLIISPRARIFQGHDWVYGTEVRKIFGNPQPGDVVALKDFKDRFLGSAMFNPHSQIVA
CCCCEECCCHHEECCCCCEEHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCHHHHHH
RRFSRRKQELNGDFFSRRISQAVELRRRRLPEETLTRLVWSESDGLPGLIVDRYADYLVV
HHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
QTLTIAMECRLPIILNVLEDLLSPRGIIVRNDSPMLAAEGISPSVRVARGQQPEPFAARS
HHHHHHHHCCHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCCEEECCCCCCCCCCCCC
GSVQFMIDLQTGQKTGLYLDQLDNYAAVARFARGRRVLDCFCNQGGFALACALAGASEVT
CCEEEEEEECCCCCCCCHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCCCEE
AVDVSQDAMDAVARNARLNGVSVQCVTDNAFDFLKKEAALVRDGGEHKWDLIILDPPSFT
EEECCHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
RNKKSVHDAMRGYKEIHLRAMKLLAPGGILSTFCCSHHAGADLFRESVLDAAIDAPATLR
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHH
LMQQHGQRADHPVLLNIPETEYLKGFTYELLPGR
HHHHHCCCCCCCEEEECCCHHHHCCCEEECCCCC
>Mature Secondary Structure 
AGLIISPRARIFQGHDWVYGTEVRKIFGNPQPGDVVALKDFKDRFLGSAMFNPHSQIVA
CCCEECCCHHEECCCCCEEHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCHHHHHH
RRFSRRKQELNGDFFSRRISQAVELRRRRLPEETLTRLVWSESDGLPGLIVDRYADYLVV
HHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
QTLTIAMECRLPIILNVLEDLLSPRGIIVRNDSPMLAAEGISPSVRVARGQQPEPFAARS
HHHHHHHHCCHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCCEEECCCCCCCCCCCCC
GSVQFMIDLQTGQKTGLYLDQLDNYAAVARFARGRRVLDCFCNQGGFALACALAGASEVT
CCEEEEEEECCCCCCCCHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCCCEE
AVDVSQDAMDAVARNARLNGVSVQCVTDNAFDFLKKEAALVRDGGEHKWDLIILDPPSFT
EEECCHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
RNKKSVHDAMRGYKEIHLRAMKLLAPGGILSTFCCSHHAGADLFRESVLDAAIDAPATLR
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHH
LMQQHGQRADHPVLLNIPETEYLKGFTYELLPGR
HHHHHCCCCCCCEEEECCCHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA