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The map label for this gene is rlmI [H]

Identifier: 187736305

GI number: 187736305

Start: 2206455

End: 2207756

Strand: Direct

Name: rlmI [H]

Synonym: Amuc_1818

Alternate gene names: 187736305

Gene position: 2206455-2207756 (Clockwise)

Preceding gene: 187736304

Following gene: 187736306

Centisome position: 82.82

GC content: 60.75

Gene sequence:

>1302_bases
ATGAATGATTTCCACAATTCCCGTGATTCCCGCCCCAGGCCCGTTTACCGGGACCGCCGTTCCCAGCCGGGCCGCCCGCG
CACAGCTCCCAAAAAGAACCTTTTTGCCAACAAGTCGCCGGAAGGGAGCGAACAATGGCTCAAGCCCTGGGTGGAGCTGA
AGTATTTCACATACAATCCGGCCGTGTTCCCCCGCATGCTCGGCTCCGTAAGCGGGGAAATAGCGCCGGGAAGCCTGGTA
AACGTCTATGACAAGAACGGTGAACTGTTCGGCGCCGGATTCTGGAATGAGTCCAGCCGCACGCCCCTGCGCATGGTGTA
CCACGGGAAGGACGTTTTTGCCGAACGTGACCTGGATGCTGCGCTGGAACGAGCCGTGAAGCTGCGCAGGGAAGTTCTGC
GCCTGGATGAAACCACTAATGCGTACCGTGTGCTGCACGGAGATTCCGACGGACTGGGCGGCCTGGTGGTGGACCGTTAC
GCGGACGTGCTGAGCCTGGAGGTGAGTACGCTGGCCGTCTGGCAGAGGCTGAACCGCTGGCTGCCCCTGCTGCACCGCCT
GTGCGGCACAAAACGCCATGTGGTGCAGGTGGATGAGGGCATTGCCCGCATGGAGGGCATCCGGGCGGAGGAGGCGCCGG
CATCCCCAGCCCCCGTCAGGCTGGTGAAGATTGTGGAAAACGGCATCACCTATGAAGTGGACTTCGCCCAGGGGCACAAG
ACGGGCTTTTTCTGTGACCAGCGGGACAACCGCCTCAAGTTCGCCTCCCTGGTGAAGGGGGCCACGGTGCTGGACCTGTG
CTGCTACAGCGGCGGCTTTTCCATTGCCGCCAAGATGCTGGGCGGAGCGGCGGAGGTAACGGCTGTGGATTTGGATGAGA
AAGCCGTCGCCATGGCTAAAAGAAACGGAAATATCAACCGTCAGCGCATCGACTTCGTGCATGCGGATGCCTTTGTCTAT
GCGCGCCAGATGGTGCGCAACGGCCGCCTGTTTGACGCCGTGCTGCTGGACCCGCCCAAATTCATCGTTGGCAGGGACGG
ATATGAAGAGGGCATCAAAAAATACCATGACCTCAATATGTTGGGGCTGCAATGCGTGCGTCCCGGCGGCCTGTTTGTCA
CCTGTTCCTGTTCCGGGCTGCTTTCCCCCGCGGAGTTTGAGCATACCGTCATCAAGGCCGCCCAGCGGCAGGGGCGCAAG
CTTCAGATTATGGCGATGACCGGCCCCGGTTGGGACCACCCTTTCCTGAGCACTTATCCGGAGGGCCGTTATCTGAAGGT
TCTGTGGGCAATTGCCCTGTAG

Upstream 100 bases:

>100_bases
TCTCTTCCCTGATGAAGCAGCCCGTGAGGGCGTATGAAGAGGCCATTGACATGGCCGTGGCCCGCTATGCCGCAGATCCA
ACCTTTCTCTCCAGCATTTC

Downstream 100 bases:

>100_bases
CTGGAGGTTGAGGCCCGGGATGCATTTCGGAAAAACCTTTCAGGCGTGTTGACGTTCTTTGCCCCTCTCTGCTTTCGTGG
TGAGGAATATGGCAAAACAA

Product: PUA domain containing protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MNDFHNSRDSRPRPVYRDRRSQPGRPRTAPKKNLFANKSPEGSEQWLKPWVELKYFTYNPAVFPRMLGSVSGEIAPGSLV
NVYDKNGELFGAGFWNESSRTPLRMVYHGKDVFAERDLDAALERAVKLRREVLRLDETTNAYRVLHGDSDGLGGLVVDRY
ADVLSLEVSTLAVWQRLNRWLPLLHRLCGTKRHVVQVDEGIARMEGIRAEEAPASPAPVRLVKIVENGITYEVDFAQGHK
TGFFCDQRDNRLKFASLVKGATVLDLCCYSGGFSIAAKMLGGAAEVTAVDLDEKAVAMAKRNGNINRQRIDFVHADAFVY
ARQMVRNGRLFDAVLLDPPKFIVGRDGYEEGIKKYHDLNMLGLQCVRPGGLFVTCSCSGLLSPAEFEHTVIKAAQRQGRK
LQIMAMTGPGWDHPFLSTYPEGRYLKVLWAIAL

Sequences:

>Translated_433_residues
MNDFHNSRDSRPRPVYRDRRSQPGRPRTAPKKNLFANKSPEGSEQWLKPWVELKYFTYNPAVFPRMLGSVSGEIAPGSLV
NVYDKNGELFGAGFWNESSRTPLRMVYHGKDVFAERDLDAALERAVKLRREVLRLDETTNAYRVLHGDSDGLGGLVVDRY
ADVLSLEVSTLAVWQRLNRWLPLLHRLCGTKRHVVQVDEGIARMEGIRAEEAPASPAPVRLVKIVENGITYEVDFAQGHK
TGFFCDQRDNRLKFASLVKGATVLDLCCYSGGFSIAAKMLGGAAEVTAVDLDEKAVAMAKRNGNINRQRIDFVHADAFVY
ARQMVRNGRLFDAVLLDPPKFIVGRDGYEEGIKKYHDLNMLGLQCVRPGGLFVTCSCSGLLSPAEFEHTVIKAAQRQGRK
LQIMAMTGPGWDHPFLSTYPEGRYLKVLWAIAL
>Mature_433_residues
MNDFHNSRDSRPRPVYRDRRSQPGRPRTAPKKNLFANKSPEGSEQWLKPWVELKYFTYNPAVFPRMLGSVSGEIAPGSLV
NVYDKNGELFGAGFWNESSRTPLRMVYHGKDVFAERDLDAALERAVKLRREVLRLDETTNAYRVLHGDSDGLGGLVVDRY
ADVLSLEVSTLAVWQRLNRWLPLLHRLCGTKRHVVQVDEGIARMEGIRAEEAPASPAPVRLVKIVENGITYEVDFAQGHK
TGFFCDQRDNRLKFASLVKGATVLDLCCYSGGFSIAAKMLGGAAEVTAVDLDEKAVAMAKRNGNINRQRIDFVHADAFVY
ARQMVRNGRLFDAVLLDPPKFIVGRDGYEEGIKKYHDLNMLGLQCVRPGGLFVTCSCSGLLSPAEFEHTVIKAAQRQGRK
LQIMAMTGPGWDHPFLSTYPEGRYLKVLWAIAL

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=376, Percent_Identity=31.3829787234043, Blast_Score=195, Evalue=6e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 48543; Mature: 48543

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDFHNSRDSRPRPVYRDRRSQPGRPRTAPKKNLFANKSPEGSEQWLKPWVELKYFTYNP
CCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCHHHEEEEEEECC
AVFPRMLGSVSGEIAPGSLVNVYDKNGELFGAGFWNESSRTPLRMVYHGKDVFAERDLDA
HHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCEEEEEECCHHHHHCCHHH
ALERAVKLRREVLRLDETTNAYRVLHGDSDGLGGLVVDRYADVLSLEVSTLAVWQRLNRW
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LPLLHRLCGTKRHVVQVDEGIARMEGIRAEEAPASPAPVRLVKIVENGITYEVDFAQGHK
HHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCEEHEEHHCCCEEEEECCCCCC
TGFFCDQRDNRLKFASLVKGATVLDLCCYSGGFSIAAKMLGGAAEVTAVDLDEKAVAMAK
CCEEECCCCCCCHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCHHHHHHHH
RNGNINRQRIDFVHADAFVYARQMVRNGRLFDAVLLDPPKFIVGRDGYEEGIKKYHDLNM
CCCCCCHHEEEEEEHHHHHHHHHHHHCCCEEEEEEECCCCEEECCCCHHHHHHHHHCCCC
LGLQCVRPGGLFVTCSCSGLLSPAEFEHTVIKAAQRQGRKLQIMAMTGPGWDHPFLSTYP
EEHEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCC
EGRYLKVLWAIAL
CCCEEEEEEEECC
>Mature Secondary Structure
MNDFHNSRDSRPRPVYRDRRSQPGRPRTAPKKNLFANKSPEGSEQWLKPWVELKYFTYNP
CCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCHHHEEEEEEECC
AVFPRMLGSVSGEIAPGSLVNVYDKNGELFGAGFWNESSRTPLRMVYHGKDVFAERDLDA
HHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCEEEEEECCHHHHHCCHHH
ALERAVKLRREVLRLDETTNAYRVLHGDSDGLGGLVVDRYADVLSLEVSTLAVWQRLNRW
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LPLLHRLCGTKRHVVQVDEGIARMEGIRAEEAPASPAPVRLVKIVENGITYEVDFAQGHK
HHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCEEHEEHHCCCEEEEECCCCCC
TGFFCDQRDNRLKFASLVKGATVLDLCCYSGGFSIAAKMLGGAAEVTAVDLDEKAVAMAK
CCEEECCCCCCCHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCHHHHHHHH
RNGNINRQRIDFVHADAFVYARQMVRNGRLFDAVLLDPPKFIVGRDGYEEGIKKYHDLNM
CCCCCCHHEEEEEEHHHHHHHHHHHHCCCEEEEEEECCCCEEECCCCHHHHHHHHHCCCC
LGLQCVRPGGLFVTCSCSGLLSPAEFEHTVIKAAQRQGRKLQIMAMTGPGWDHPFLSTYP
EEHEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCC
EGRYLKVLWAIAL
CCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA