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The map label for this gene is amyA [C]

Identifier: 187736299

GI number: 187736299

Start: 2198164

End: 2199642

Strand: Reverse

Name: amyA [C]

Synonym: Amuc_1812

Alternate gene names: 187736299

Gene position: 2199642-2198164 (Counterclockwise)

Preceding gene: 187736300

Following gene: 187736297

Centisome position: 82.57

GC content: 52.74

Gene sequence:

>1479_bases
ATGAAAAACGGAATCATGATGCAGTATTTTGAGTGGAATCTGCCCAACGACGGACAGTTTTGGAACAAGTTGAAGGAAGA
CGCCCCCCACCTGGAGGAAATGGGTGTTACGGCCGTCTGGATTCCTCCAGCCTACAAAGGGAAGGAACAAAATGACGTAG
GATACGGCACCTATGATCTTTTTGACCTGGGGGAATTCGACCAGAAAAACACCGTCCGCACCAAATACGGCACCAGGCAG
GAACTCCAGGAGGCCATCAAGGCTCTTCATGAACACCATGTAGGCGTTTATCTGGATGCCGTCATGAACCACAAAGCAGG
GGCGGACTATACGGAAAAATTCATGGCCAAGGAAGTGGACCAGCAGAACCGCGACAAGGAAATTACGGATGCCTACGAGA
TTGAAGGCTGGACCGGCTTCAACTTTCCCGGCCGCGGAAACAAATATTCCGACTTCAAATGGCACTGGTACCACTTCACC
GGAACGGACTATGACGCACGCACGGAAAAAACCTCCATTTTCAAGATTATGGGAGACGGCAAAAGCTGGAGCGAAGGCGT
GGATGAAGAAAACGGCAACTACGACTACCTTATGTTCGCCAATCTGGACTTCAACCACCCGGAAGTGGTGAAAGAAATGG
AAAGATGGGGAATATGGGTATCCCGGGAACTGGATCTGGACGGCATGAGGCTGGACGCCATCAAGCATATCAATGACGAA
TTCATCAGGAAATTCCTGGCAGCCGTCCGCAAGGAAAGGGGAGCAGACTTCTACGCCGTGGGCGAATACTGGAAACAGGA
TCTGGAATCCCTGGACGATTACCTCAAGGAGGAACGCTACAAAGTGGACCTGTTTGACGTCCCCCTGCACTACAACATGT
ACCAGGCCTCCAAGCAGGGCCGCGATTACGACCTTTCCAAAATTCTGGACGGAACCCTGGTTCAGAACCACCCTACGCTG
GCCGTTACCTTTGTGGACAACCATGATTCCCAGTGGGGCAGTTCCCTGGAATCAGCTGTGGAAGACTGGTTCAAACCCTC
CGCCTATGCGCTCATCCTGCTCATGAAGGAAGGTTATCCCTGCATCTTCTACGGGGACTATTACGGTGTGAGCGGCAACC
CGCCCATGCACCGCGCCATCATCGACAATCTGCTGGAAATCCGTAAAAACCATGCTTTCGGGGAACAAAATTACTACTTT
GACCACCCGAACACCATCGGCTTCACCCGTGTGGGAGATGAAGAGCACCCGCATTCCGGCGTGGCTGTGCTCATTTCCAA
TGGAGAGGATGGAGACAAAGTCATGAACGTGGGCAAGCAGCACGCAGGGGAAACTTGGAAGGAAGCCACCGGCAACGTGG
AGGAAACCGTCTCCATTGACGGGGAAGGCAACGGCCGGTTTCTTGTCCACGGCGGCAATGTGGCCGTATGGATTCCGGAA
AACGCTTTACAGGACGCCCGGAAGGAAGACGGAAAATAA

Upstream 100 bases:

>100_bases
GGATTCATCATCCGGAACCTCGCCCGGGAAAACCGCTTTTTCCACCGGACAGCCCCTGGAAGCACGACAACCCGAACGGT
ATGACAAGGAATCAGATCAA

Downstream 100 bases:

>100_bases
CGCAACTCCCGCCCGCAAATTGAAGAAACGGCCTGTTCTCCATTCCGGACTGTTTTTCCGGAATGAGGTTCAGGCCGTTC
TGACTTTAATCACCAGTTTC

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: Exo-maltohexaohydrolase; G6-amylase; Maltohexaose-producing amylase [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MKNGIMMQYFEWNLPNDGQFWNKLKEDAPHLEEMGVTAVWIPPAYKGKEQNDVGYGTYDLFDLGEFDQKNTVRTKYGTRQ
ELQEAIKALHEHHVGVYLDAVMNHKAGADYTEKFMAKEVDQQNRDKEITDAYEIEGWTGFNFPGRGNKYSDFKWHWYHFT
GTDYDARTEKTSIFKIMGDGKSWSEGVDEENGNYDYLMFANLDFNHPEVVKEMERWGIWVSRELDLDGMRLDAIKHINDE
FIRKFLAAVRKERGADFYAVGEYWKQDLESLDDYLKEERYKVDLFDVPLHYNMYQASKQGRDYDLSKILDGTLVQNHPTL
AVTFVDNHDSQWGSSLESAVEDWFKPSAYALILLMKEGYPCIFYGDYYGVSGNPPMHRAIIDNLLEIRKNHAFGEQNYYF
DHPNTIGFTRVGDEEHPHSGVAVLISNGEDGDKVMNVGKQHAGETWKEATGNVEETVSIDGEGNGRFLVHGGNVAVWIPE
NALQDARKEDGK

Sequences:

>Translated_492_residues
MKNGIMMQYFEWNLPNDGQFWNKLKEDAPHLEEMGVTAVWIPPAYKGKEQNDVGYGTYDLFDLGEFDQKNTVRTKYGTRQ
ELQEAIKALHEHHVGVYLDAVMNHKAGADYTEKFMAKEVDQQNRDKEITDAYEIEGWTGFNFPGRGNKYSDFKWHWYHFT
GTDYDARTEKTSIFKIMGDGKSWSEGVDEENGNYDYLMFANLDFNHPEVVKEMERWGIWVSRELDLDGMRLDAIKHINDE
FIRKFLAAVRKERGADFYAVGEYWKQDLESLDDYLKEERYKVDLFDVPLHYNMYQASKQGRDYDLSKILDGTLVQNHPTL
AVTFVDNHDSQWGSSLESAVEDWFKPSAYALILLMKEGYPCIFYGDYYGVSGNPPMHRAIIDNLLEIRKNHAFGEQNYYF
DHPNTIGFTRVGDEEHPHSGVAVLISNGEDGDKVMNVGKQHAGETWKEATGNVEETVSIDGEGNGRFLVHGGNVAVWIPE
NALQDARKEDGK
>Mature_492_residues
MKNGIMMQYFEWNLPNDGQFWNKLKEDAPHLEEMGVTAVWIPPAYKGKEQNDVGYGTYDLFDLGEFDQKNTVRTKYGTRQ
ELQEAIKALHEHHVGVYLDAVMNHKAGADYTEKFMAKEVDQQNRDKEITDAYEIEGWTGFNFPGRGNKYSDFKWHWYHFT
GTDYDARTEKTSIFKIMGDGKSWSEGVDEENGNYDYLMFANLDFNHPEVVKEMERWGIWVSRELDLDGMRLDAIKHINDE
FIRKFLAAVRKERGADFYAVGEYWKQDLESLDDYLKEERYKVDLFDVPLHYNMYQASKQGRDYDLSKILDGTLVQNHPTL
AVTFVDNHDSQWGSSLESAVEDWFKPSAYALILLMKEGYPCIFYGDYYGVSGNPPMHRAIIDNLLEIRKNHAFGEQNYYF
DHPNTIGFTRVGDEEHPHSGVAVLISNGEDGDKVMNVGKQHAGETWKEATGNVEETVSIDGEGNGRFLVHGGNVAVWIPE
NALQDARKEDGK

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=497, Percent_Identity=41.851106639839, Blast_Score=404, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR015237
- InterPro:   IPR006046
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF09154 DUF1939 [H]

EC number: =3.2.1.98 [H]

Molecular weight: Translated: 56720; Mature: 56720

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNGIMMQYFEWNLPNDGQFWNKLKEDAPHLEEMGVTAVWIPPAYKGKEQNDVGYGTYDL
CCCCEEEEEEECCCCCCCHHHHHHHHCCCCHHHCCCEEEEECCCCCCCCCCCCCCCCEEE
FDLGEFDQKNTVRTKYGTRQELQEAIKALHEHHVGVYLDAVMNHKAGADYTEKFMAKEVD
CCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCHHHHHHHHHHH
QQNRDKEITDAYEIEGWTGFNFPGRGNKYSDFKWHWYHFTGTDYDARTEKTSIFKIMGDG
HCCCCCCCCCCEEECCEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECC
KSWSEGVDEENGNYDYLMFANLDFNHPEVVKEMERWGIWVSRELDLDGMRLDAIKHINDE
CCHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCHH
FIRKFLAAVRKERGADFYAVGEYWKQDLESLDDYLKEERYKVDLFDVPLHYNMYQASKQG
HHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHCCC
RDYDLSKILDGTLVQNHPTLAVTFVDNHDSQWGSSLESAVEDWFKPSAYALILLMKEGYP
CCCCHHHHHCCCEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCC
CIFYGDYYGVSGNPPMHRAIIDNLLEIRKNHAFGEQNYYFDHPNTIGFTRVGDEEHPHSG
EEEECCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEECCCCCCCCCC
VAVLISNGEDGDKVMNVGKQHAGETWKEATGNVEETVSIDGEGNGRFLVHGGNVAVWIPE
EEEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCEEEEEECCCCCCEEEEECCCEEEEECC
NALQDARKEDGK
HHHHHHHHCCCC
>Mature Secondary Structure
MKNGIMMQYFEWNLPNDGQFWNKLKEDAPHLEEMGVTAVWIPPAYKGKEQNDVGYGTYDL
CCCCEEEEEEECCCCCCCHHHHHHHHCCCCHHHCCCEEEEECCCCCCCCCCCCCCCCEEE
FDLGEFDQKNTVRTKYGTRQELQEAIKALHEHHVGVYLDAVMNHKAGADYTEKFMAKEVD
CCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCHHHHHHHHHHH
QQNRDKEITDAYEIEGWTGFNFPGRGNKYSDFKWHWYHFTGTDYDARTEKTSIFKIMGDG
HCCCCCCCCCCEEECCEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECC
KSWSEGVDEENGNYDYLMFANLDFNHPEVVKEMERWGIWVSRELDLDGMRLDAIKHINDE
CCHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCHH
FIRKFLAAVRKERGADFYAVGEYWKQDLESLDDYLKEERYKVDLFDVPLHYNMYQASKQG
HHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHCCC
RDYDLSKILDGTLVQNHPTLAVTFVDNHDSQWGSSLESAVEDWFKPSAYALILLMKEGYP
CCCCHHHHHCCCEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCC
CIFYGDYYGVSGNPPMHRAIIDNLLEIRKNHAFGEQNYYFDHPNTIGFTRVGDEEHPHSG
EEEECCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEECCCCCCCCCC
VAVLISNGEDGDKVMNVGKQHAGETWKEATGNVEETVSIDGEGNGRFLVHGGNVAVWIPE
EEEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCEEEEEECCCCCCEEEEECCCEEEEECC
NALQDARKEDGK
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3258152 [H]