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The map label for this gene is dapE [H]

Identifier: 187736246

GI number: 187736246

Start: 2141787

End: 2143184

Strand: Direct

Name: dapE [H]

Synonym: Amuc_1759

Alternate gene names: 187736246

Gene position: 2141787-2143184 (Clockwise)

Preceding gene: 187736238

Following gene: 187736247

Centisome position: 80.39

GC content: 57.8

Gene sequence:

>1398_bases
ATGGAATGCCACACTTTACCGGTTGATTTGAAGCCCCAGGCATGGTCTGATGAAGGCATGAACAGACTGGATCAATTAGC
CACCTTGTTGAGATTTCCAAGCATTTCCGCACAGAAGGAACACGCCCGGGACGTGAGTGATTGCGCGGACTGGCTGGTAG
ACAAATTGTCCGGCATGGGATTGGAAGCAAAGGCCTGTAAAACGCCCCTCCATCCCATTGTGCTGGCCCGCAGCCCGCGG
GAGGAAGGAAAGCCCACCGTGCTGATTTACGGCCATTACGACGTGCAGCCCGTAGATCCTGTGGAATTATGGGAATCCGA
CCCGTTTGAACCGGAAGTGAGGGACGGGAAGATTTACGCCCGCGGCGCTACGGACAACAAGGGGCAGCTGTTCGCCCATA
TTCTGGGCGTGGAGGAATTGCTCTGCCAGAACGGAGGCCACCTCCCCGTAAATGTGATTTTCCTTCTGGAAGGGGAGGAA
GAAGTGGGCAGCGGCAGTCTTTCCCAGTTTATCAAGGAGCACAGGGAAGAGCTGGCCTGCGACGTTATTGTAGTTTCCGA
TACCGGCATGGCCGCTCCGGACACCCCTACGTTTTCCTACGGGCTGCGCGGCCTGGCCGGGGCGGAAATTATCGTCAAAG
GCCCTTCCGCGGATCTTCATTCCGGCGTATTCGGAGGAGCGGTGGCCAATCCCATCGCCGCTCTTGCGGAAATAATCGCC
TCCTTCCACGATGAAGAGGGGCGTGTGGCCGTGAGAGGGTTTTACGACGGCGTGGAACCTATCGCCACATGGGAACGCAG
CATGTGGGCCACGGTTCCCGGCATGAGCAATGAGGAGCTGGCTCAGCTGACGGGCGTGGAAACCGTGGTGACGGAGGCCG
GTTTCACCGGAGCGGAATGTATTTTTGCCCGCCCGACGCTGGAAATCAACGGTATCGGCGGAGGTTACCAGGGTGAGGGA
AGCAAGACGATTATCCCCAGCCATGCCTTTGCCAAAATTTCATGCCGCCTGGTGCCGGGACAGCAGCCTGAAAGGATTGC
AACCCTGCTGGAAGAACATGTAAAGAAACATTCCCCCAAGGGCGTCACGGTGGAATTCAGGCGGGGCCATGGCGGGAATG
CTTACGTGTGCGATCCGAATTCCGAGTTCGGTCTTGCCGCCCAGCAGGCGCTGGAAGAGGCTTTTGGCCACAAACCCGTG
CTGGTAAGGGAAGGGGGCAGCATCCCGATCATTGAAGAGATGAAGCGCGTGCTGGGAGCGGATGCCCTGATGCTGGGCAT
GTGCCTTCCGGATGCCCGCATCCATTCCCCCAATGAGAACTTTCCCGTGGATTTGTTCTCCAAGGGCATTGACATGAGCC
AGATTCTTCTGCGCCGCCTGGGGCAGATCAAGCATTGA

Upstream 100 bases:

>100_bases
ATAATCCTATATAAAGGAACAAAACACACGCGAGAGCAATCAAATATTGTTCCCGGCGCCATGTGCGGGCTCCATTTTTT
GAAAAAAGGCCCGTTCCGGC

Downstream 100 bases:

>100_bases
CCTTTTTATGGACCAGCCTGTAGCCGCTGAAATAGTCAACCTGCACAAGACCTTTAAAACAGGTCTTGGCAAACCCGTGG
TGCATGCCGTGCGGGGGATA

Product: peptidase M20

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MECHTLPVDLKPQAWSDEGMNRLDQLATLLRFPSISAQKEHARDVSDCADWLVDKLSGMGLEAKACKTPLHPIVLARSPR
EEGKPTVLIYGHYDVQPVDPVELWESDPFEPEVRDGKIYARGATDNKGQLFAHILGVEELLCQNGGHLPVNVIFLLEGEE
EVGSGSLSQFIKEHREELACDVIVVSDTGMAAPDTPTFSYGLRGLAGAEIIVKGPSADLHSGVFGGAVANPIAALAEIIA
SFHDEEGRVAVRGFYDGVEPIATWERSMWATVPGMSNEELAQLTGVETVVTEAGFTGAECIFARPTLEINGIGGGYQGEG
SKTIIPSHAFAKISCRLVPGQQPERIATLLEEHVKKHSPKGVTVEFRRGHGGNAYVCDPNSEFGLAAQQALEEAFGHKPV
LVREGGSIPIIEEMKRVLGADALMLGMCLPDARIHSPNENFPVDLFSKGIDMSQILLRRLGQIKH

Sequences:

>Translated_465_residues
MECHTLPVDLKPQAWSDEGMNRLDQLATLLRFPSISAQKEHARDVSDCADWLVDKLSGMGLEAKACKTPLHPIVLARSPR
EEGKPTVLIYGHYDVQPVDPVELWESDPFEPEVRDGKIYARGATDNKGQLFAHILGVEELLCQNGGHLPVNVIFLLEGEE
EVGSGSLSQFIKEHREELACDVIVVSDTGMAAPDTPTFSYGLRGLAGAEIIVKGPSADLHSGVFGGAVANPIAALAEIIA
SFHDEEGRVAVRGFYDGVEPIATWERSMWATVPGMSNEELAQLTGVETVVTEAGFTGAECIFARPTLEINGIGGGYQGEG
SKTIIPSHAFAKISCRLVPGQQPERIATLLEEHVKKHSPKGVTVEFRRGHGGNAYVCDPNSEFGLAAQQALEEAFGHKPV
LVREGGSIPIIEEMKRVLGADALMLGMCLPDARIHSPNENFPVDLFSKGIDMSQILLRRLGQIKH
>Mature_465_residues
MECHTLPVDLKPQAWSDEGMNRLDQLATLLRFPSISAQKEHARDVSDCADWLVDKLSGMGLEAKACKTPLHPIVLARSPR
EEGKPTVLIYGHYDVQPVDPVELWESDPFEPEVRDGKIYARGATDNKGQLFAHILGVEELLCQNGGHLPVNVIFLLEGEE
EVGSGSLSQFIKEHREELACDVIVVSDTGMAAPDTPTFSYGLRGLAGAEIIVKGPSADLHSGVFGGAVANPIAALAEIIA
SFHDEEGRVAVRGFYDGVEPIATWERSMWATVPGMSNEELAQLTGVETVVTEAGFTGAECIFARPTLEINGIGGGYQGEG
SKTIIPSHAFAKISCRLVPGQQPERIATLLEEHVKKHSPKGVTVEFRRGHGGNAYVCDPNSEFGLAAQQALEEAFGHKPV
LVREGGSIPIIEEMKRVLGADALMLGMCLPDARIHSPNENFPVDLFSKGIDMSQILLRRLGQIKH

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=469, Percent_Identity=32.1961620469083, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI21071039, Length=402, Percent_Identity=30.3482587064677, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI271398277, Length=324, Percent_Identity=31.1728395061728, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1788816, Length=136, Percent_Identity=30.1470588235294, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17563146, Length=459, Percent_Identity=30.718954248366, Blast_Score=217, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI193211059, Length=451, Percent_Identity=29.490022172949, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321159, Length=464, Percent_Identity=32.3275862068966, Blast_Score=234, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6319758, Length=458, Percent_Identity=27.5109170305677, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24585880, Length=457, Percent_Identity=30.6345733041575, Blast_Score=211, Evalue=8e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 50333; Mature: 50333

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MECHTLPVDLKPQAWSDEGMNRLDQLATLLRFPSISAQKEHARDVSDCADWLVDKLSGMG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHCCC
LEAKACKTPLHPIVLARSPREEGKPTVLIYGHYDVQPVDPVELWESDPFEPEVRDGKIYA
CCHHHHCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCEEEE
RGATDNKGQLFAHILGVEELLCQNGGHLPVNVIFLLEGEEEVGSGSLSQFIKEHREELAC
ECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHEE
DVIVVSDTGMAAPDTPTFSYGLRGLAGAEIIVKGPSADLHSGVFGGAVANPIAALAEIIA
EEEEEECCCCCCCCCCCHHHCCCCCCCCEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHH
SFHDEEGRVAVRGFYDGVEPIATWERSMWATVPGMSNEELAQLTGVETVVTEAGFTGAEC
HHCCCCCCEEEEECCCCCCHHHHHHHHHEEECCCCCCHHHHHHHCHHHHHHHCCCCCCEE
IFARPTLEINGIGGGYQGEGSKTIIPSHAFAKISCRLVPGQQPERIATLLEEHVKKHSPK
EEECCCEEEECCCCCCCCCCCCEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCC
GVTVEFRRGHGGNAYVCDPNSEFGLAAQQALEEAFGHKPVLVREGGSIPIIEEMKRVLGA
CCEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCC
DALMLGMCLPDARIHSPNENFPVDLFSKGIDMSQILLRRLGQIKH
CHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MECHTLPVDLKPQAWSDEGMNRLDQLATLLRFPSISAQKEHARDVSDCADWLVDKLSGMG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHCCC
LEAKACKTPLHPIVLARSPREEGKPTVLIYGHYDVQPVDPVELWESDPFEPEVRDGKIYA
CCHHHHCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCEEEE
RGATDNKGQLFAHILGVEELLCQNGGHLPVNVIFLLEGEEEVGSGSLSQFIKEHREELAC
ECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHEE
DVIVVSDTGMAAPDTPTFSYGLRGLAGAEIIVKGPSADLHSGVFGGAVANPIAALAEIIA
EEEEEECCCCCCCCCCCHHHCCCCCCCCEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHH
SFHDEEGRVAVRGFYDGVEPIATWERSMWATVPGMSNEELAQLTGVETVVTEAGFTGAEC
HHCCCCCCEEEEECCCCCCHHHHHHHHHEEECCCCCCHHHHHHHCHHHHHHHCCCCCCEE
IFARPTLEINGIGGGYQGEGSKTIIPSHAFAKISCRLVPGQQPERIATLLEEHVKKHSPK
EEECCCEEEECCCCCCCCCCCCEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCC
GVTVEFRRGHGGNAYVCDPNSEFGLAAQQALEEAFGHKPVLVREGGSIPIIEEMKRVLGA
CCEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCC
DALMLGMCLPDARIHSPNENFPVDLFSKGIDMSQILLRRLGQIKH
CHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA