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The map label for this gene is thrS [H]

Identifier: 187736219

GI number: 187736219

Start: 2114403

End: 2116295

Strand: Direct

Name: thrS [H]

Synonym: Amuc_1732

Alternate gene names: 187736219

Gene position: 2114403-2116295 (Clockwise)

Preceding gene: 187736211

Following gene: 187736220

Centisome position: 79.37

GC content: 61.54

Gene sequence:

>1893_bases
ATGTCCGAACACAAGGAAAGAAAGACTCTTGAAGAACGCCAACAGATGTCTGATCTGGAACGGCTGCGCCACTCCTGCGC
GCACGTTCTGGCTACGGCCATTTGCCGCCTCTGGCCGGATGCCCAGCTGGCCGGCGGGCCTGCCGTGGATAACGGTTTTT
ATTACGACGTGGAGCTGGACCACCGCATCAGCACAGAGGATTTTGAACGCATTGAGGAGGAGATGAAAAAAGTGGTGAAG
GAAAACCAGGTTTTCCAGAAGGAAGTCATTTCCCGCGCGGATGCCATGAAGATGGCGGAATCCGGGGAGCTGGGCGCCCT
GGGCCCCCGCAGCGAGCCTTCCCGCTTCAAGATTGACCTGCTGAACGACATCCCGGAAGACGAGGAGATTTCCCTCTACC
GCAACGGGGATTTTACGGACTTGTGCGCCGGTCCCCACGTGGGCCGCACGGGCAACTGCAAGGCATTCAAAATCATGAGC
GTGGCGAGCGCCTTCTACAAGGGGGACAAAAACCGCCCCATGCTCCAGCGCATTTACGGCACCTGCTTCCCGAACCGCAC
CCAGCTTGACGAACACCTGGAACGGCTGGAAGAGGCGCGGCGCCGCGACCACCGCAAACTGGGCCGCGAACTGGGCCTGT
TCTGCATTGACGAATCCGTGGGGCAGGGGCTCATCCTCTGGAAGCCCAAGGGGGCTCTCATCCGCCGTTCCCTGCAGGAC
TTCATCACGGAAGAGCTGGACAAGCTGGGCTACTCCCAGGTGTACACGCCCAACATCGGCAAGCTGGACCTGTACCGCAC
GTCCGGACACTTCCCGTATTATCAGGAAAGCCAGTACGCGCCCATCCCAGAACGGGACGCCATGGAAAAACTCAGCCAGG
AGGGGGCTTCCTGCGCGGAACTGTTCAACGGCCTGGCCACCGGCACCATTGAGGGCTATATGCTCAAGCCGATGAACTGC
CCCCACCACATCAAGATTTACGCGAATGACGCCCACTCCTACCGGGACCTTCCCGTGCGGCTGGCGGAATTCGGCACGGT
GTACCGTTGGGAACAGAGCGGAGAGCTGGGCGGCATGACGCGCGTGCGCGGTTTCACGCAGGATGACGCCCATATCTTCT
GCACGCCGGACCAGCTTGCCGGGGAAATCCGCCAGTGCCTGGGCATCGTGAAAACCATTTTCGGTACGCTGGGCATGACG
GACTACCGCGTGCGCCTTTCCATGCGCGACCCGGAAAGCGACAAATACGTGGGTTCTCCGGAAAATTGGGACAAAGCGGA
ACAGGCTCTGCGGGAAGCTGCGGAATGGCTGGGGGCGGACTACAGCGAGGAAGCTGGGGAAGCCGCCTTCTACGGCCCCA
AGATCGACTTCATCGTGCGCGACGCCATCGGCCGCGAATGGCAGCTGGGAACCGTGCAGGTGGACTACAACCTGCCGGAA
CGCTTTGACCTCCATTACACCGGAGCGGACAACAAGCCGCACCGGCCCGTGATGGTGCACCGCGCCCCCTTCGGCTCCAT
GGAACGCTTCACGGGGCTTCTGATTGAACACTTTGAAGGCAAATTCCCCACCTGGCTTTCCCCGGAACAGGTGCGCGTGC
TTCCCATCTCCGACAAGGTGACGGACGTGGCCGCCGCGCATCGAACCGCCCTGGCCGCCAGAGGCGTGCGCGTCACGGTG
GACGAAACGCCGGACAAAATAGGCGCCAAAATCCGCAATGCCCGTCTGGACCGCGTTCCCTACATGCTGGTGCTCGGCCA
GCGGGAGGCGGAAGACGGCACCGTCTCCGTCCGCCACCGGGACAAGGGGGATCTGGGCGCGATGCCCTTTGAGCAATTCG
CGGACCTCGTAGCCCGGGAAATCGCGGAGCGTCATATTTCTCCCGTGATTTGA

Upstream 100 bases:

>100_bases
GAGGAAAGGGCTTTCCCGCGGCGTTCATGCCTTGAATTTTCGGGAAGAAGGTCTTTTTGCATTTGCCAACAGCGTTTAAG
AGAGGTTTACTGATGCGCGT

Downstream 100 bases:

>100_bases
GTTGCCAAGTTATTCCTTTTAGTTTAGTAGTATCCGGACGTGCCAACCAAGCCAACGAAGAATAATGACGGAAATCGCCG
CCGTGACCGCGGCGATCAGA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MSEHKERKTLEERQQMSDLERLRHSCAHVLATAICRLWPDAQLAGGPAVDNGFYYDVELDHRISTEDFERIEEEMKKVVK
ENQVFQKEVISRADAMKMAESGELGALGPRSEPSRFKIDLLNDIPEDEEISLYRNGDFTDLCAGPHVGRTGNCKAFKIMS
VASAFYKGDKNRPMLQRIYGTCFPNRTQLDEHLERLEEARRRDHRKLGRELGLFCIDESVGQGLILWKPKGALIRRSLQD
FITEELDKLGYSQVYTPNIGKLDLYRTSGHFPYYQESQYAPIPERDAMEKLSQEGASCAELFNGLATGTIEGYMLKPMNC
PHHIKIYANDAHSYRDLPVRLAEFGTVYRWEQSGELGGMTRVRGFTQDDAHIFCTPDQLAGEIRQCLGIVKTIFGTLGMT
DYRVRLSMRDPESDKYVGSPENWDKAEQALREAAEWLGADYSEEAGEAAFYGPKIDFIVRDAIGREWQLGTVQVDYNLPE
RFDLHYTGADNKPHRPVMVHRAPFGSMERFTGLLIEHFEGKFPTWLSPEQVRVLPISDKVTDVAAAHRTALAARGVRVTV
DETPDKIGAKIRNARLDRVPYMLVLGQREAEDGTVSVRHRDKGDLGAMPFEQFADLVAREIAERHISPVI

Sequences:

>Translated_630_residues
MSEHKERKTLEERQQMSDLERLRHSCAHVLATAICRLWPDAQLAGGPAVDNGFYYDVELDHRISTEDFERIEEEMKKVVK
ENQVFQKEVISRADAMKMAESGELGALGPRSEPSRFKIDLLNDIPEDEEISLYRNGDFTDLCAGPHVGRTGNCKAFKIMS
VASAFYKGDKNRPMLQRIYGTCFPNRTQLDEHLERLEEARRRDHRKLGRELGLFCIDESVGQGLILWKPKGALIRRSLQD
FITEELDKLGYSQVYTPNIGKLDLYRTSGHFPYYQESQYAPIPERDAMEKLSQEGASCAELFNGLATGTIEGYMLKPMNC
PHHIKIYANDAHSYRDLPVRLAEFGTVYRWEQSGELGGMTRVRGFTQDDAHIFCTPDQLAGEIRQCLGIVKTIFGTLGMT
DYRVRLSMRDPESDKYVGSPENWDKAEQALREAAEWLGADYSEEAGEAAFYGPKIDFIVRDAIGREWQLGTVQVDYNLPE
RFDLHYTGADNKPHRPVMVHRAPFGSMERFTGLLIEHFEGKFPTWLSPEQVRVLPISDKVTDVAAAHRTALAARGVRVTV
DETPDKIGAKIRNARLDRVPYMLVLGQREAEDGTVSVRHRDKGDLGAMPFEQFADLVAREIAERHISPVI
>Mature_629_residues
SEHKERKTLEERQQMSDLERLRHSCAHVLATAICRLWPDAQLAGGPAVDNGFYYDVELDHRISTEDFERIEEEMKKVVKE
NQVFQKEVISRADAMKMAESGELGALGPRSEPSRFKIDLLNDIPEDEEISLYRNGDFTDLCAGPHVGRTGNCKAFKIMSV
ASAFYKGDKNRPMLQRIYGTCFPNRTQLDEHLERLEEARRRDHRKLGRELGLFCIDESVGQGLILWKPKGALIRRSLQDF
ITEELDKLGYSQVYTPNIGKLDLYRTSGHFPYYQESQYAPIPERDAMEKLSQEGASCAELFNGLATGTIEGYMLKPMNCP
HHIKIYANDAHSYRDLPVRLAEFGTVYRWEQSGELGGMTRVRGFTQDDAHIFCTPDQLAGEIRQCLGIVKTIFGTLGMTD
YRVRLSMRDPESDKYVGSPENWDKAEQALREAAEWLGADYSEEAGEAAFYGPKIDFIVRDAIGREWQLGTVQVDYNLPER
FDLHYTGADNKPHRPVMVHRAPFGSMERFTGLLIEHFEGKFPTWLSPEQVRVLPISDKVTDVAAAHRTALAARGVRVTVD
ETPDKIGAKIRNARLDRVPYMLVLGQREAEDGTVSVRHRDKGDLGAMPFEQFADLVAREIAERHISPVI

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=581, Percent_Identity=38.382099827883, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI38570111, Length=581, Percent_Identity=36.6609294320138, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI20070344, Length=586, Percent_Identity=36.1774744027304, Blast_Score=348, Evalue=9e-96,
Organism=Escherichia coli, GI1788013, Length=610, Percent_Identity=41.8032786885246, Blast_Score=477, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI71984192, Length=601, Percent_Identity=37.1048252911814, Blast_Score=403, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71984184, Length=601, Percent_Identity=37.1048252911814, Blast_Score=403, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6322112, Length=593, Percent_Identity=37.9426644182125, Blast_Score=413, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6322654, Length=441, Percent_Identity=33.1065759637188, Blast_Score=248, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24583842, Length=580, Percent_Identity=39.1379310344828, Blast_Score=420, Evalue=1e-117,
Organism=Drosophila melanogaster, GI28574141, Length=580, Percent_Identity=39.1379310344828, Blast_Score=420, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24583839, Length=580, Percent_Identity=39.1379310344828, Blast_Score=420, Evalue=1e-117,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 71582; Mature: 71451

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEHKERKTLEERQQMSDLERLRHSCAHVLATAICRLWPDAQLAGGPAVDNGFYYDVELD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEC
HRISTEDFERIEEEMKKVVKENQVFQKEVISRADAMKMAESGELGALGPRSEPSRFKIDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEH
LNDIPEDEEISLYRNGDFTDLCAGPHVGRTGNCKAFKIMSVASAFYKGDKNRPMLQRIYG
HHCCCCCCCEEEEECCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
TCFPNRTQLDEHLERLEEARRRDHRKLGRELGLFCIDESVGQGLILWKPKGALIRRSLQD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCEEEECCCHHHHHHHHHH
FITEELDKLGYSQVYTPNIGKLDLYRTSGHFPYYQESQYAPIPERDAMEKLSQEGASCAE
HHHHHHHHCCCCEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHH
LFNGLATGTIEGYMLKPMNCPHHIKIYANDAHSYRDLPVRLAEFGTVYRWEQSGELGGMT
HHCCHHHCCCCCEEECCCCCCCEEEEEECCCCCCCCCCHHHHHCCCEEEECCCCCCCCHH
RVRGFTQDDAHIFCTPDQLAGEIRQCLGIVKTIFGTLGMTDYRVRLSMRDPESDKYVGSP
HHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCC
ENWDKAEQALREAAEWLGADYSEEAGEAAFYGPKIDFIVRDAIGREWQLGTVQVDYNLPE
CCHHHHHHHHHHHHHHHCCCCCHHCCCEEEECCCEEEEEHHHCCCCEEEEEEEECCCCCC
RFDLHYTGADNKPHRPVMVHRAPFGSMERFTGLLIEHFEGKFPTWLSPEQVRVLPISDKV
CEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHH
TDVAAAHRTALAARGVRVTVDETPDKIGAKIRNARLDRVPYMLVLGQREAEDGTVSVRHR
HHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEC
DKGDLGAMPFEQFADLVAREIAERHISPVI
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SEHKERKTLEERQQMSDLERLRHSCAHVLATAICRLWPDAQLAGGPAVDNGFYYDVELD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEC
HRISTEDFERIEEEMKKVVKENQVFQKEVISRADAMKMAESGELGALGPRSEPSRFKIDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEH
LNDIPEDEEISLYRNGDFTDLCAGPHVGRTGNCKAFKIMSVASAFYKGDKNRPMLQRIYG
HHCCCCCCCEEEEECCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
TCFPNRTQLDEHLERLEEARRRDHRKLGRELGLFCIDESVGQGLILWKPKGALIRRSLQD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCEEEECCCHHHHHHHHHH
FITEELDKLGYSQVYTPNIGKLDLYRTSGHFPYYQESQYAPIPERDAMEKLSQEGASCAE
HHHHHHHHCCCCEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHH
LFNGLATGTIEGYMLKPMNCPHHIKIYANDAHSYRDLPVRLAEFGTVYRWEQSGELGGMT
HHCCHHHCCCCCEEECCCCCCCEEEEEECCCCCCCCCCHHHHHCCCEEEECCCCCCCCHH
RVRGFTQDDAHIFCTPDQLAGEIRQCLGIVKTIFGTLGMTDYRVRLSMRDPESDKYVGSP
HHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCC
ENWDKAEQALREAAEWLGADYSEEAGEAAFYGPKIDFIVRDAIGREWQLGTVQVDYNLPE
CCHHHHHHHHHHHHHHHCCCCCHHCCCEEEECCCEEEEEHHHCCCCEEEEEEEECCCCCC
RFDLHYTGADNKPHRPVMVHRAPFGSMERFTGLLIEHFEGKFPTWLSPEQVRVLPISDKV
CEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHH
TDVAAAHRTALAARGVRVTVDETPDKIGAKIRNARLDRVPYMLVLGQREAEDGTVSVRHR
HHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEC
DKGDLGAMPFEQFADLVAREIAERHISPVI
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]