Click here to switch to the map view.

The map label for this gene is folD

Identifier: 187736130

GI number: 187736130

Start: 1984050

End: 1984925

Strand: Direct

Name: folD

Synonym: Amuc_1641

Alternate gene names: 187736130

Gene position: 1984050-1984925 (Clockwise)

Preceding gene: 187736129

Following gene: 187736131

Centisome position: 74.47

GC content: 57.42

Gene sequence:

>876_bases
ATGCAAATCATCGATGGAAAACAAGTAGCTTCCCAGGTGCTGGAAGAGGTTGAAAGGGATATTGCCCGGCTCAGGGAACA
GGGAATAACGCCCGGCTTGGCCGTGGTTCTGGTAGGGGAAGATCCCGCTTCCCAGGTATATGTAAATTCCAAGGTGAAAA
AGTGTGCGGAGCTGGGGATGAATTCCCGTAAAATAGTTCTCCCTTCCGACGCCTCCCAGGAAGAACTGCTCCGGATTGTC
CGTGAATTGAACGTGGATCCTTCCGTCCACGGCATACTGGTGCAAAGCCCGCCTCCCCCCCATATCGACGAGGCGGCCGT
GGTCCTGGAAATAGACCCTGCCAAGGATGTGGACGGCTTCCATCCGGAGAATGTCGCCAAGCTGGTGCTGGAAGATGAGA
CCGGTTTTGTACCCTGTACCCCTCTGGGCTGTATGAGGCTGTTAAAGGCCGCCGGCATTGAAACGGCGGGAGCGAATGCC
GTGGTCATTGGCCGCAGCATGATTGTGGGCAAACCGATGGCGCATCTTTTGATGTCCAAACAGGCAAACGCTACCGTAAC
CGTGGCTCATTCCCGTACGAAAAACCTGCCGGAACTTTGCCGTTCCGCTGATATCATCGTGGCAGCCATAGGCCGGCCCG
CTTTTGTGACGGCTGATTTTGTGAAAGACGGCGCCGTAGTGGTGGATGTGGGCATCAACCGCGTGGAAGATGCCTCCGCC
AAGCGCGGCTACCGGATTGTGGGAGACGTAGCCTATGACGAGGTGGCTCCCAAGTGCCGGGCCATTACTCCCGTGCCCGG
CGGCGTGGGGCCGATGACTATCGCCATGTTGATGGCTAATACGGTCAAAGCGTGCCGCCAGCAGACGGGAGCCTGA

Upstream 100 bases:

>100_bases
CGGACGCGACGGATGAACAGGTGGCGGATTATGCCCGTCTTCTGGCCGGGCTGCTTGGCGAGGGGCTTCCGGACTTCCCG
CCTCTTAACCAATCATTATT

Downstream 100 bases:

>100_bases
CGAAAATGCCGTGACGTCCGTTTTTCTTGTCAAATGAAGGAAAGTAGGGCAGTATTCAGTTACCATTATGTCAAGTAAAT
TACTCGAAGGCAAAGTTGGT

Product: Methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MQIIDGKQVASQVLEEVERDIARLREQGITPGLAVVLVGEDPASQVYVNSKVKKCAELGMNSRKIVLPSDASQEELLRIV
RELNVDPSVHGILVQSPPPPHIDEAAVVLEIDPAKDVDGFHPENVAKLVLEDETGFVPCTPLGCMRLLKAAGIETAGANA
VVIGRSMIVGKPMAHLLMSKQANATVTVAHSRTKNLPELCRSADIIVAAIGRPAFVTADFVKDGAVVVDVGINRVEDASA
KRGYRIVGDVAYDEVAPKCRAITPVPGGVGPMTIAMLMANTVKACRQQTGA

Sequences:

>Translated_291_residues
MQIIDGKQVASQVLEEVERDIARLREQGITPGLAVVLVGEDPASQVYVNSKVKKCAELGMNSRKIVLPSDASQEELLRIV
RELNVDPSVHGILVQSPPPPHIDEAAVVLEIDPAKDVDGFHPENVAKLVLEDETGFVPCTPLGCMRLLKAAGIETAGANA
VVIGRSMIVGKPMAHLLMSKQANATVTVAHSRTKNLPELCRSADIIVAAIGRPAFVTADFVKDGAVVVDVGINRVEDASA
KRGYRIVGDVAYDEVAPKCRAITPVPGGVGPMTIAMLMANTVKACRQQTGA
>Mature_291_residues
MQIIDGKQVASQVLEEVERDIARLREQGITPGLAVVLVGEDPASQVYVNSKVKKCAELGMNSRKIVLPSDASQEELLRIV
RELNVDPSVHGILVQSPPPPHIDEAAVVLEIDPAKDVDGFHPENVAKLVLEDETGFVPCTPLGCMRLLKAAGIETAGANA
VVIGRSMIVGKPMAHLLMSKQANATVTVAHSRTKNLPELCRSADIIVAAIGRPAFVTADFVKDGAVVVDVGINRVEDASA
KRGYRIVGDVAYDEVAPKCRAITPVPGGVGPMTIAMLMANTVKACRQQTGA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=291, Percent_Identity=43.6426116838488, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=43.8775510204082, Blast_Score=228, Evalue=4e-60,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=44.8979591836735, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI36796743, Length=224, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1786741, Length=285, Percent_Identity=49.1228070175439, Blast_Score=258, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17568735, Length=295, Percent_Identity=41.0169491525424, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6319558, Length=291, Percent_Identity=45.0171821305842, Blast_Score=257, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=43.9597315436242, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6322933, Length=319, Percent_Identity=25.705329153605, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17136816, Length=296, Percent_Identity=48.9864864864865, Blast_Score=259, Evalue=1e-69,
Organism=Drosophila melanogaster, GI17136818, Length=296, Percent_Identity=48.9864864864865, Blast_Score=259, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24645718, Length=288, Percent_Identity=47.5694444444444, Blast_Score=251, Evalue=4e-67,
Organism=Drosophila melanogaster, GI17137370, Length=288, Percent_Identity=47.5694444444444, Blast_Score=251, Evalue=4e-67,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=47.4048442906574, Blast_Score=251, Evalue=4e-67,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=47.4048442906574, Blast_Score=251, Evalue=4e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_AKKM8 (B2UM17)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001878242.1
- ProteinModelPortal:   B2UM17
- SMR:   B2UM17
- GeneID:   6274645
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_1641
- HOGENOM:   HBG328751
- OMA:   CLERISP
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30847; Mature: 30847

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIIDGKQVASQVLEEVERDIARLREQGITPGLAVVLVGEDPASQVYVNSKVKKCAELGM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHCCC
NSRKIVLPSDASQEELLRIVRELNVDPSVHGILVQSPPPPHIDEAAVVLEIDPAKDVDGF
CCCEEEECCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCC
HPENVAKLVLEDETGFVPCTPLGCMRLLKAAGIETAGANAVVIGRSMIVGKPMAHLLMSK
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECHHHHCCHHHHHHHHC
QANATVTVAHSRTKNLPELCRSADIIVAAIGRPAFVTADFVKDGAVVVDVGINRVEDASA
CCCCEEEEECCCCCCHHHHHCCCCEEEEECCCCCEEEHHHHCCCCEEEEECCCCCCCHHH
KRGYRIVGDVAYDEVAPKCRAITPVPGGVGPMTIAMLMANTVKACRQQTGA
CCCCEEEEECHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQIIDGKQVASQVLEEVERDIARLREQGITPGLAVVLVGEDPASQVYVNSKVKKCAELGM
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHCCC
NSRKIVLPSDASQEELLRIVRELNVDPSVHGILVQSPPPPHIDEAAVVLEIDPAKDVDGF
CCCEEEECCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCC
HPENVAKLVLEDETGFVPCTPLGCMRLLKAAGIETAGANAVVIGRSMIVGKPMAHLLMSK
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECHHHHCCHHHHHHHHC
QANATVTVAHSRTKNLPELCRSADIIVAAIGRPAFVTADFVKDGAVVVDVGINRVEDASA
CCCCEEEEECCCCCCHHHHHCCCCEEEEECCCCCEEEHHHHCCCCEEEEECCCCCCCHHH
KRGYRIVGDVAYDEVAPKCRAITPVPGGVGPMTIAMLMANTVKACRQQTGA
CCCCEEEEECHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA