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The map label for this gene is fabI [H]

Identifier: 187736131

GI number: 187736131

Start: 1984993

End: 1985775

Strand: Direct

Name: fabI [H]

Synonym: Amuc_1642

Alternate gene names: 187736131

Gene position: 1984993-1985775 (Clockwise)

Preceding gene: 187736130

Following gene: 187736135

Centisome position: 74.51

GC content: 53.9

Gene sequence:

>783_bases
ATGTCAAGTAAATTACTCGAAGGCAAAGTTGGTGTCGTAGTCGGCGTAGCGAACAAGCGCAGCATCGCGTTCGCAATTGC
CAAGGCGTGGCATGAAGCGGGCGCAAAGCTCATCTTCAATTATCAGGGAGAACGTCTCAAGGACGGAGTTCTCAAGCTGA
TCCATGAGAACTTCGGAGAGGATGTTCCCGTTTATGATCTGGATGTCAGCAGTGATGAATCTGTTAACGCTTTCTTTGAA
AGGGTCCGCCAGCATACGGATAAAGTAGATTGTCTGCTGCATTCCGTGGCTTTTGCCCCGAAGGAGGCTCTTGGCGGCAG
TTTCCTGGAAACGGGCCGCGAGGCGTTCAAGATTTCCCTGGATATCTCCGCTTATTCCCTGGTAGCTCTTTCCCGCGCCG
TGGAACCCATGATGTCCGACAACGGCAGCATTTTGGCGATGTCCTATCTGGGATCCGAAAAAGTGGTTCCTAACTACAAT
CTGATGGGAGTTTCCAAAGCTGCCCTGGAAGCCTGCACCCGTTATCTGGCTTATGATTTGGGCCGTTCCCGCGGTATCCG
TGTCAATTGCATCAGTGCAGGCCCCATGCAGACGCTGGCTGCTCGCGGCGTTTCCGGTTTTAACACCATGTTCAAGGTGT
ATGAGGAGCATGCCCCTCTGGGCCGTTCCTGCACGGGTGAGGAATTGGGAGCCACCGGCGTTTTCCTGGCCAGTGACGGC
GCAGCCGCAATCACCGGGCAGGTGATTTACGTGGACGGCGGTTATCAGATCATGGGAATGTAA

Upstream 100 bases:

>100_bases
GTCAAAGCGTGCCGCCAGCAGACGGGAGCCTGACGAAAATGCCGTGACGTCCGTTTTTCTTGTCAAATGAAGGAAAGTAG
GGCAGTATTCAGTTACCATT

Downstream 100 bases:

>100_bases
CGCGTTCCTTGCGGAAGCTTTCTTCCTGTTCACAAGCCGTACCGGTTTTTCCGGTACGGCTTGTTTACGGTTTCATGGAG
AAGGTAAGGGGGGCTGGGGG

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSSKLLEGKVGVVVGVANKRSIAFAIAKAWHEAGAKLIFNYQGERLKDGVLKLIHENFGEDVPVYDLDVSSDESVNAFFE
RVRQHTDKVDCLLHSVAFAPKEALGGSFLETGREAFKISLDISAYSLVALSRAVEPMMSDNGSILAMSYLGSEKVVPNYN
LMGVSKAALEACTRYLAYDLGRSRGIRVNCISAGPMQTLAARGVSGFNTMFKVYEEHAPLGRSCTGEELGATGVFLASDG
AAAITGQVIYVDGGYQIMGM

Sequences:

>Translated_260_residues
MSSKLLEGKVGVVVGVANKRSIAFAIAKAWHEAGAKLIFNYQGERLKDGVLKLIHENFGEDVPVYDLDVSSDESVNAFFE
RVRQHTDKVDCLLHSVAFAPKEALGGSFLETGREAFKISLDISAYSLVALSRAVEPMMSDNGSILAMSYLGSEKVVPNYN
LMGVSKAALEACTRYLAYDLGRSRGIRVNCISAGPMQTLAARGVSGFNTMFKVYEEHAPLGRSCTGEELGATGVFLASDG
AAAITGQVIYVDGGYQIMGM
>Mature_259_residues
SSKLLEGKVGVVVGVANKRSIAFAIAKAWHEAGAKLIFNYQGERLKDGVLKLIHENFGEDVPVYDLDVSSDESVNAFFER
VRQHTDKVDCLLHSVAFAPKEALGGSFLETGREAFKISLDISAYSLVALSRAVEPMMSDNGSILAMSYLGSEKVVPNYNL
MGVSKAALEACTRYLAYDLGRSRGIRVNCISAGPMQTLAARGVSGFNTMFKVYEEHAPLGRSCTGEELGATGVFLASDGA
AAITGQVIYVDGGYQIMGM

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031737, Length=252, Percent_Identity=27.3809523809524, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI66933014, Length=202, Percent_Identity=28.2178217821782, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI32483357, Length=253, Percent_Identity=26.4822134387352, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787545, Length=257, Percent_Identity=43.579766536965, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI87082100, Length=264, Percent_Identity=27.6515151515151, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787905, Length=251, Percent_Identity=25.8964143426295, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787335, Length=254, Percent_Identity=28.3464566929134, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71994604, Length=205, Percent_Identity=27.3170731707317, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71994600, Length=205, Percent_Identity=26.8292682926829, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=268, Percent_Identity=27.6119402985075, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17562904, Length=201, Percent_Identity=23.8805970149254, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21355319, Length=253, Percent_Identity=29.6442687747036, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27880; Mature: 27749

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKLLEGKVGVVVGVANKRSIAFAIAKAWHEAGAKLIFNYQGERLKDGVLKLIHENFGE
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCC
DVPVYDLDVSSDESVNAFFERVRQHTDKVDCLLHSVAFAPKEALGGSFLETGREAFKISL
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHCCCEEEEEEE
DISAYSLVALSRAVEPMMSDNGSILAMSYLGSEKVVPNYNLMGVSKAALEACTRYLAYDL
ECHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GRSRGIRVNCISAGPMQTLAARGVSGFNTMFKVYEEHAPLGRSCTGEELGATGVFLASDG
CCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCC
AAAITGQVIYVDGGYQIMGM
CEEEEEEEEEEECCEEEECC
>Mature Secondary Structure 
SSKLLEGKVGVVVGVANKRSIAFAIAKAWHEAGAKLIFNYQGERLKDGVLKLIHENFGE
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCC
DVPVYDLDVSSDESVNAFFERVRQHTDKVDCLLHSVAFAPKEALGGSFLETGREAFKISL
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHCCCEEEEEEE
DISAYSLVALSRAVEPMMSDNGSILAMSYLGSEKVVPNYNLMGVSKAALEACTRYLAYDL
ECHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GRSRGIRVNCISAGPMQTLAARGVSGFNTMFKVYEEHAPLGRSCTGEELGATGVFLASDG
CCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCC
AAAITGQVIYVDGGYQIMGM
CEEEEEEEEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]