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The map label for this gene is cca [H]

Identifier: 187736125

GI number: 187736125

Start: 1975844

End: 1977238

Strand: Direct

Name: cca [H]

Synonym: Amuc_1636

Alternate gene names: 187736125

Gene position: 1975844-1977238 (Clockwise)

Preceding gene: 187736124

Following gene: 187736126

Centisome position: 74.17

GC content: 56.56

Gene sequence:

>1395_bases
ATGATCCGTTTCATGGAGGGGATTCTGTTGAGAAAGGCCGCGGAGGACGTGGCCCGGGTTCTTTCCGGAGCAGGACACAC
CGTATATTTTGTGGGCGGCTGTGTTCGGGACAGGTTGCTGGGATATTCCGTGAAGGATATCGATATTGCCACCTCCGCCC
GCCCGGATGAAGTGTTGCGCCTGTTTCCGGATGCCTGGGAAGTGGGCGCCGCCTTCGGCGTGGTGCTGGTACGCCGCGAA
GGTTTTTCCTTTGAGGTGGCGACCTTCCGCAGAGACGGAAGCTACAGGGATGGCCGGAGGCCGGAGGACGTCTGTTTCAC
GGATGCGGAAGAAGATGCCCGGCGGCGTGATTTTACCATGAACGGCCTCTTTGAAGATCCGTTTTCTTCGCCTCCGGGCC
GTGTGGTGGATTATGTAGGCGGAGTGGAGGATATCCGGGCCCGCGTATTGAGGTGCATCGGGGAGCCGGACCGTCGTTTT
GAAGAAGATTCCCTGCGGCTGATGCGCGCCGTACGTTTTGCCGTTACTAGGGAAGTTCAGGTGGAGAAGGAGACTTGCTC
CTCCATTTTCAGGAACGCTCCGCTGCTGGCCCGGATTTCCCCGGAACGCATCCGGGAGGAACTGGACAGGATTTTATTGT
CTCCCGGAAGGAAACGGGGTGTGGAAATGCTGGTGGAAACGGGGTTGATGAAGCATGTCATTCCGGAAATATACGATATG
ATAGGCTGTACGCAACCTCCCCAATGGCATCCGGAAGGAGACGTATACACCCATACGCTGATGATGCTGGATGCGCTGGG
AAGGGACGGCTCCCCCGTGTCTTTGAAGCTGGCCCTGGGGGTTCTGCTGCATGACATCGGGAAACCTCCCTGCCGTCAGG
TGGATGAAACGGGGCGCATCCGTTTTTCCGGTCATGACAAGGAAGGTTCTTCCATGGCCAGGGTCATTTTAAGAAGGCTG
AAATATTCCAATGCCGTGGTGGATGCGGTGTGTTCCATGGTGGAGCGCCATATGCGCTTCATCAACGTGAGGCAGATGAG
GAAATCTACGCTGCGAATGTTCATGAACGCTCCTCATTTCCGGGATGAACTGGAACTCCACCGCCTGGATTGCCTTTCCT
CCAACGGACTGATGGACAACTGGCAATTTGTCAGGGACTTCATGGATTCCTACCGGAATGCCCCTCTGGTTCCTCCTCCT
CTGGTGACCGGGCGTGATCTGGTGAATCTGGGGCTGTCCCCGGGGCCTGATTTCGGGAAATGGCTGTCCCGTCTGCAGGA
ACTTCAGTTGGAGGGAACGCTCCGGACCAGGGAGGAAGCCCTGCTGCAGCTGGGACAAATTGCCCCGGTGGAACAGAATG
CGCTTGCAGAATACCTGAAAAAGTTAGCATTATAA

Upstream 100 bases:

>100_bases
CTGTTTCCCGGCCTGATAGGCCGCGGCCCGCGTTCCCTTCCGCCCCCTCCGGTGCGGAGGACCATTCTCTTTCCGCTCCT
CCTTTGAGGCCCATGCCTGA

Downstream 100 bases:

>100_bases
GCGTCAAAATTTGATTATTTCAACTCATGCGTCCTGTCATTTATCAACTATTTGTTCGTCATTTTTCCAATATGGAGACC
CATGGAGTTGACTGGGGGAG

Product: polynucleotide adenylyltransferase/metal dependent phosphohydrolase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MIRFMEGILLRKAAEDVARVLSGAGHTVYFVGGCVRDRLLGYSVKDIDIATSARPDEVLRLFPDAWEVGAAFGVVLVRRE
GFSFEVATFRRDGSYRDGRRPEDVCFTDAEEDARRRDFTMNGLFEDPFSSPPGRVVDYVGGVEDIRARVLRCIGEPDRRF
EEDSLRLMRAVRFAVTREVQVEKETCSSIFRNAPLLARISPERIREELDRILLSPGRKRGVEMLVETGLMKHVIPEIYDM
IGCTQPPQWHPEGDVYTHTLMMLDALGRDGSPVSLKLALGVLLHDIGKPPCRQVDETGRIRFSGHDKEGSSMARVILRRL
KYSNAVVDAVCSMVERHMRFINVRQMRKSTLRMFMNAPHFRDELELHRLDCLSSNGLMDNWQFVRDFMDSYRNAPLVPPP
LVTGRDLVNLGLSPGPDFGKWLSRLQELQLEGTLRTREEALLQLGQIAPVEQNALAEYLKKLAL

Sequences:

>Translated_464_residues
MIRFMEGILLRKAAEDVARVLSGAGHTVYFVGGCVRDRLLGYSVKDIDIATSARPDEVLRLFPDAWEVGAAFGVVLVRRE
GFSFEVATFRRDGSYRDGRRPEDVCFTDAEEDARRRDFTMNGLFEDPFSSPPGRVVDYVGGVEDIRARVLRCIGEPDRRF
EEDSLRLMRAVRFAVTREVQVEKETCSSIFRNAPLLARISPERIREELDRILLSPGRKRGVEMLVETGLMKHVIPEIYDM
IGCTQPPQWHPEGDVYTHTLMMLDALGRDGSPVSLKLALGVLLHDIGKPPCRQVDETGRIRFSGHDKEGSSMARVILRRL
KYSNAVVDAVCSMVERHMRFINVRQMRKSTLRMFMNAPHFRDELELHRLDCLSSNGLMDNWQFVRDFMDSYRNAPLVPPP
LVTGRDLVNLGLSPGPDFGKWLSRLQELQLEGTLRTREEALLQLGQIAPVEQNALAEYLKKLAL
>Mature_464_residues
MIRFMEGILLRKAAEDVARVLSGAGHTVYFVGGCVRDRLLGYSVKDIDIATSARPDEVLRLFPDAWEVGAAFGVVLVRRE
GFSFEVATFRRDGSYRDGRRPEDVCFTDAEEDARRRDFTMNGLFEDPFSSPPGRVVDYVGGVEDIRARVLRCIGEPDRRF
EEDSLRLMRAVRFAVTREVQVEKETCSSIFRNAPLLARISPERIREELDRILLSPGRKRGVEMLVETGLMKHVIPEIYDM
IGCTQPPQWHPEGDVYTHTLMMLDALGRDGSPVSLKLALGVLLHDIGKPPCRQVDETGRIRFSGHDKEGSSMARVILRRL
KYSNAVVDAVCSMVERHMRFINVRQMRKSTLRMFMNAPHFRDELELHRLDCLSSNGLMDNWQFVRDFMDSYRNAPLVPPP
LVTGRDLVNLGLSPGPDFGKWLSRLQELQLEGTLRTREEALLQLGQIAPVEQNALAEYLKKLAL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=240, Percent_Identity=32.5, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI87081691, Length=228, Percent_Identity=39.0350877192982, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1789436, Length=322, Percent_Identity=31.055900621118, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71995920, Length=202, Percent_Identity=33.6633663366337, Blast_Score=99, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6321016, Length=489, Percent_Identity=23.1083844580777, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281360112, Length=311, Percent_Identity=31.5112540192926, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21357337, Length=311, Percent_Identity=31.5112540192926, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 52748; Mature: 52748

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRFMEGILLRKAAEDVARVLSGAGHTVYFVGGCVRDRLLGYSVKDIDIATSARPDEVLR
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCCCCEEECCCCCHHHHHH
LFPDAWEVGAAFGVVLVRREGFSFEVATFRRDGSYRDGRRPEDVCFTDAEEDARRRDFTM
HCCCHHHHHHHHHHHEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCC
NGLFEDPFSSPPGRVVDYVGGVEDIRARVLRCIGEPDRRFEEDSLRLMRAVRFAVTREVQ
CCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHH
VEKETCSSIFRNAPLLARISPERIREELDRILLSPGRKRGVEMLVETGLMKHVIPEIYDM
HHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGCTQPPQWHPEGDVYTHTLMMLDALGRDGSPVSLKLALGVLLHDIGKPPCRQVDETGRI
HCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCHHCCCCCCCE
RFSGHDKEGSSMARVILRRLKYSNAVVDAVCSMVERHMRFINVRQMRKSTLRMFMNAPHF
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RDELELHRLDCLSSNGLMDNWQFVRDFMDSYRNAPLVPPPLVTGRDLVNLGLSPGPDFGK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCHHH
WLSRLQELQLEGTLRTREEALLQLGQIAPVEQNALAEYLKKLAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIRFMEGILLRKAAEDVARVLSGAGHTVYFVGGCVRDRLLGYSVKDIDIATSARPDEVLR
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCCCCEEECCCCCHHHHHH
LFPDAWEVGAAFGVVLVRREGFSFEVATFRRDGSYRDGRRPEDVCFTDAEEDARRRDFTM
HCCCHHHHHHHHHHHEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCC
NGLFEDPFSSPPGRVVDYVGGVEDIRARVLRCIGEPDRRFEEDSLRLMRAVRFAVTREVQ
CCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHH
VEKETCSSIFRNAPLLARISPERIREELDRILLSPGRKRGVEMLVETGLMKHVIPEIYDM
HHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGCTQPPQWHPEGDVYTHTLMMLDALGRDGSPVSLKLALGVLLHDIGKPPCRQVDETGRI
HCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCHHCCCCCCCE
RFSGHDKEGSSMARVILRRLKYSNAVVDAVCSMVERHMRFINVRQMRKSTLRMFMNAPHF
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RDELELHRLDCLSSNGLMDNWQFVRDFMDSYRNAPLVPPPLVTGRDLVNLGLSPGPDFGK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCHHH
WLSRLQELQLEGTLRTREEALLQLGQIAPVEQNALAEYLKKLAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA