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The map label for this gene is epsE [H]

Identifier: 187736074

GI number: 187736074

Start: 1905728

End: 1907386

Strand: Reverse

Name: epsE [H]

Synonym: Amuc_1585

Alternate gene names: 187736074

Gene position: 1907386-1905728 (Counterclockwise)

Preceding gene: 187736075

Following gene: 187736073

Centisome position: 71.6

GC content: 56.9

Gene sequence:

>1659_bases
ATGGACACCAACCTCACACTGGAACTTTTCATCGGCCGGGGAATGATTGACAAATCCCTGGCAAAGGACATCAAGGAGGA
AATGATCGCCTCCGGCAAGGAGCTGCCGGAAGTGCTTGCAGACTTCGGCATCATCGGCAGCAAGGATGATATCTGGCAGA
TGATTGCCAGCGACCTGGGTACGGAATTCATTACACTGGACAACTTCCAGCCGGATCCGAACGTGCAGAACATGATGCCG
GCCACGCTCGTGCGCCTGCACGGGGCGCTCCCTGTGCGGCATGGTCCGGAAGGCCTGTACGTCTGCCTGGTGGATCCCCT
GAATCCCCAGACGGTGGAAGACCTGCGCTTCGCCCTCGGCCAGGACATCCATGTTCTGGTAGCGCCGGATTACCAGATTT
CCGAACGCATCAATGAGCTTTATGGAGGCGAATCCGCCGCCATGTCCGACCTGATGCAGGAGCTGAACAACATGCAGGTC
AACAATGAGACGGAGGACTCCGCCGCCGCTCCCGTCATCCGCTTTGTGGACCTCGTCATTACGCAGGCCATCAAGGAAAA
GGCCTCCGACATTCACTTCGAACCTTTTGAGAAGGAATTCAAAATCCGCTACCGTGTGGACGGCGCCCTGTATGAAATGC
AGCCTCCCCCCGTCCACCTGTCCGTGCCGGTCATTTCCCGCGTCAAAGTCATGGCGAACATGAACATCGCGGAACGCCGC
ATTCCGCAGGACGGACGCATCGTCAAGCAGATAGGAAACCGTTCCGTGGACATGCGCGTTTCCTCCCTTCCCACTCAGTA
CGGAGAATCCGTGGTGCTCCGCGTTCTGGACCGCTCTTCCGTCAACTTGAACATGGACAACCTGGGGCTTCCCGCGCATA
TCCACGAATATATTCTGGATACGGTCCACAAGCCCAACGGCATTTTCATCGTTACCGGCCCCACCGGCGCCGGCAAGACA
ACTACGCTGTATGCCGCCCTGCGTGAAATCAATACCATTGATTCCAAGGTGCTGACGGCGGAAGACCCTGTTGAATACGA
TATTGACGGCATCATCCAGATTCCTATCAATGAAGCCATCGGCCTGGACTTCCCAATGGTGCTCCGCGCCTTCCTGCGAC
AGGACCCGGACCGTATTCTGGTGGGGGAAATGCGAGACATGGCAACAGCGCAGATCGCCATCCAGGCATCCCTGACGGGT
CACCTGGTTCTCTCCACCCTGCACACGAACGACTCCGCCGGAGCCATTACGCGACTGGTGGACATGGGATGCGAACCTTT
CCTGGTGGCGGCTTCCCTGGAAGGGGTGCTTGCACAGCGCCTGGTGCGCACCATCTGTCCGGACTGCCGCACGCCGTATG
AACCCTCATCCACCATCCTCTCCCAGCTTGGCGTCTCTCCCTATGAACTGGGAGACAAGCACTTTTTCACGGGCCGAGGC
TGTGATAAATGCTCCAATTCCGGCTACAGGGGCCGCAAGGGGATTTATGAGCTCCTGGATATTAACGATACCCTGCGCGA
CATGATTACGGATCGCGCTCCTTCCGTGGTGCTGAAGCAGAAAGCCATTGAAATGGGCATGTCCACGCTGCGGGAAGACG
GGCTGAGAAATATTTATGACGGCAACACCACCATTGAAGAAGTGCTGAAATATACTTAA

Upstream 100 bases:

>100_bases
GTACGCAAAGTGCTGGCCGGCCTTACTTCTCCGGAAGAAGTGCTGAACGTCACCATGGGAGACGCCAACTGATTTTTCCC
CGCTATTCATTCTGCTTATT

Downstream 100 bases:

>100_bases
CGGACAGCCGGACGCCGTTGCTTCCCCTTATTTCAACCACTAAGAAAAAACACTTAAAATACAACATATATGCCTAAATA
TCAATACACAGCACTTGACC

Product: type II secretion system protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MDTNLTLELFIGRGMIDKSLAKDIKEEMIASGKELPEVLADFGIIGSKDDIWQMIASDLGTEFITLDNFQPDPNVQNMMP
ATLVRLHGALPVRHGPEGLYVCLVDPLNPQTVEDLRFALGQDIHVLVAPDYQISERINELYGGESAAMSDLMQELNNMQV
NNETEDSAAAPVIRFVDLVITQAIKEKASDIHFEPFEKEFKIRYRVDGALYEMQPPPVHLSVPVISRVKVMANMNIAERR
IPQDGRIVKQIGNRSVDMRVSSLPTQYGESVVLRVLDRSSVNLNMDNLGLPAHIHEYILDTVHKPNGIFIVTGPTGAGKT
TTLYAALREINTIDSKVLTAEDPVEYDIDGIIQIPINEAIGLDFPMVLRAFLRQDPDRILVGEMRDMATAQIAIQASLTG
HLVLSTLHTNDSAGAITRLVDMGCEPFLVAASLEGVLAQRLVRTICPDCRTPYEPSSTILSQLGVSPYELGDKHFFTGRG
CDKCSNSGYRGRKGIYELLDINDTLRDMITDRAPSVVLKQKAIEMGMSTLREDGLRNIYDGNTTIEEVLKYT

Sequences:

>Translated_552_residues
MDTNLTLELFIGRGMIDKSLAKDIKEEMIASGKELPEVLADFGIIGSKDDIWQMIASDLGTEFITLDNFQPDPNVQNMMP
ATLVRLHGALPVRHGPEGLYVCLVDPLNPQTVEDLRFALGQDIHVLVAPDYQISERINELYGGESAAMSDLMQELNNMQV
NNETEDSAAAPVIRFVDLVITQAIKEKASDIHFEPFEKEFKIRYRVDGALYEMQPPPVHLSVPVISRVKVMANMNIAERR
IPQDGRIVKQIGNRSVDMRVSSLPTQYGESVVLRVLDRSSVNLNMDNLGLPAHIHEYILDTVHKPNGIFIVTGPTGAGKT
TTLYAALREINTIDSKVLTAEDPVEYDIDGIIQIPINEAIGLDFPMVLRAFLRQDPDRILVGEMRDMATAQIAIQASLTG
HLVLSTLHTNDSAGAITRLVDMGCEPFLVAASLEGVLAQRLVRTICPDCRTPYEPSSTILSQLGVSPYELGDKHFFTGRG
CDKCSNSGYRGRKGIYELLDINDTLRDMITDRAPSVVLKQKAIEMGMSTLREDGLRNIYDGNTTIEEVLKYT
>Mature_552_residues
MDTNLTLELFIGRGMIDKSLAKDIKEEMIASGKELPEVLADFGIIGSKDDIWQMIASDLGTEFITLDNFQPDPNVQNMMP
ATLVRLHGALPVRHGPEGLYVCLVDPLNPQTVEDLRFALGQDIHVLVAPDYQISERINELYGGESAAMSDLMQELNNMQV
NNETEDSAAAPVIRFVDLVITQAIKEKASDIHFEPFEKEFKIRYRVDGALYEMQPPPVHLSVPVISRVKVMANMNIAERR
IPQDGRIVKQIGNRSVDMRVSSLPTQYGESVVLRVLDRSSVNLNMDNLGLPAHIHEYILDTVHKPNGIFIVTGPTGAGKT
TTLYAALREINTIDSKVLTAEDPVEYDIDGIIQIPINEAIGLDFPMVLRAFLRQDPDRILVGEMRDMATAQIAIQASLTG
HLVLSTLHTNDSAGAITRLVDMGCEPFLVAASLEGVLAQRLVRTICPDCRTPYEPSSTILSQLGVSPYELGDKHFFTGRG
CDKCSNSGYRGRKGIYELLDINDTLRDMITDRAPSVVLKQKAIEMGMSTLREDGLRNIYDGNTTIEEVLKYT

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=405, Percent_Identity=47.1604938271605, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1786296, Length=473, Percent_Identity=35.0951374207188, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI87082188, Length=194, Percent_Identity=41.2371134020619, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 61182; Mature: 61182

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTNLTLELFIGRGMIDKSLAKDIKEEMIASGKELPEVLADFGIIGSKDDIWQMIASDLG
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHC
TEFITLDNFQPDPNVQNMMPATLVRLHGALPVRHGPEGLYVCLVDPLNPQTVEDLRFALG
CCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHC
QDIHVLVAPDYQISERINELYGGESAAMSDLMQELNNMQVNNETEDSAAAPVIRFVDLVI
CCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
TQAIKEKASDIHFEPFEKEFKIRYRVDGALYEMQPPPVHLSVPVISRVKVMANMNIAERR
HHHHHHHHHCCCCCCCCCCEEEEEEECCEEEECCCCCEEEECCHHHHHHHHHCCCHHHHC
IPQDGRIVKQIGNRSVDMRVSSLPTQYGESVVLRVLDRSSVNLNMDNLGLPAHIHEYILD
CCCCCHHHHHHCCCCEEEEHHHCCHHHCHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHH
TVHKPNGIFIVTGPTGAGKTTTLYAALREINTIDSKVLTAEDPVEYDIDGIIQIPINEAI
HCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCEEEEECCCCC
GLDFPMVLRAFLRQDPDRILVGEMRDMATAQIAIQASLTGHLVLSTLHTNDSAGAITRLV
CCCHHHHHHHHHHCCCCCEEHHHHHHHHHHEEEEEEECCCEEEEEEEECCCCHHHHHHHH
DMGCEPFLVAASLEGVLAQRLVRTICPDCRTPYEPSSTILSQLGVSPYELGDKHFFTGRG
HCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHCCCCCEECCCC
CDKCSNSGYRGRKGIYELLDINDTLRDMITDRAPSVVLKQKAIEMGMSTLREDGLRNIYD
CHHHCCCCCCCCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHCC
GNTTIEEVLKYT
CCCHHHHHHCCC
>Mature Secondary Structure
MDTNLTLELFIGRGMIDKSLAKDIKEEMIASGKELPEVLADFGIIGSKDDIWQMIASDLG
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHC
TEFITLDNFQPDPNVQNMMPATLVRLHGALPVRHGPEGLYVCLVDPLNPQTVEDLRFALG
CCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHC
QDIHVLVAPDYQISERINELYGGESAAMSDLMQELNNMQVNNETEDSAAAPVIRFVDLVI
CCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
TQAIKEKASDIHFEPFEKEFKIRYRVDGALYEMQPPPVHLSVPVISRVKVMANMNIAERR
HHHHHHHHHCCCCCCCCCCEEEEEEECCEEEECCCCCEEEECCHHHHHHHHHCCCHHHHC
IPQDGRIVKQIGNRSVDMRVSSLPTQYGESVVLRVLDRSSVNLNMDNLGLPAHIHEYILD
CCCCCHHHHHHCCCCEEEEHHHCCHHHCHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHH
TVHKPNGIFIVTGPTGAGKTTTLYAALREINTIDSKVLTAEDPVEYDIDGIIQIPINEAI
HCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCEEEEECCCCC
GLDFPMVLRAFLRQDPDRILVGEMRDMATAQIAIQASLTGHLVLSTLHTNDSAGAITRLV
CCCHHHHHHHHHHCCCCCEEHHHHHHHHHHEEEEEEECCCEEEEEEEECCCCHHHHHHHH
DMGCEPFLVAASLEGVLAQRLVRTICPDCRTPYEPSSTILSQLGVSPYELGDKHFFTGRG
HCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHCCCCCEECCCC
CDKCSNSGYRGRKGIYELLDINDTLRDMITDRAPSVVLKQKAIEMGMSTLREDGLRNIYD
CHHHCCCCCCCCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHCC
GNTTIEEVLKYT
CCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]