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The map label for this gene is pflB [H]

Identifier: 187736033

GI number: 187736033

Start: 1854516

End: 1856783

Strand: Reverse

Name: pflB [H]

Synonym: Amuc_1543

Alternate gene names: 187736033

Gene position: 1856783-1854516 (Counterclockwise)

Preceding gene: 187736038

Following gene: 187736032

Centisome position: 69.7

GC content: 57.94

Gene sequence:

>2268_bases
ATGATCAGCATAGTAAAAGACTTGAAGGAGTCCACCGCCCTTCCGCAGGAATGGCAGGGATTCAAGCCGGGAACATGGAC
GGAGTCCATTGACGTACGGGATTTCATCCAGCATAACTACACGCCCTATTCCGGCAATGAAGAGTTTCTTTCCGGCCCCT
CCCAGCGCACGCTTCGTTTATGGGACGAGCTGAAAGTCCTGCTGAAACGGGAAATAGACAACGGCGGCGTGCTGGATGCT
GATGAAAAAGTGGTATCCTCCATCACATCCCACAAGCCCGGCTACATTGACAAGGAACTGGAAGTGGTCGTAGGCCTCCA
GACGGACGCCCCCCTGAAAAGGGCCCTGATGCCCTTTGGCGGCTTGCGCATGGCCCAACAGGCCCTGGAATCCTACGGGT
TCAAGATGTGCGAGAAAACCGCGGACATTTTCAAAAAGATACGCAAGACGCACAATGAAGGCGTTTTCGACGCCTACACT
TCCGATATCCGGGCGGCCCGTTCCGCCGGGATTATTACCGGCCTTCCGGACGCCTATGGCCGCGGCCGCATCATCGGGGA
CTACCGCCGGGTGGCCCTGTACGGCACGGATAAGCTGATTGCCGAACGCCGCAAGGACCTCAAAAACCGTGAACATTCCC
CCCTTACGGATGAACTGATCCGTCTGCGCGAAGAAATGAGCGAACAAATACGCGCCCTGGAAGAACTCGCCCAGCTGGGC
GCCTCCTACGGCTGCGACCTCACTCGCCCCGCCGCAAACGCCCGCGAAGCCGTGCAATGGACGTACCTGGGCTACCTGGC
TGCCGTGAAAGAACAGAATGGAGCGGCCATGTCCCTGGGCCGCGTTTCCACTTTCTTCGACATTTATTTCACCCGCGACC
TGGAACAGGGGCTCATCACGGAAGAGGAAGTTCAGGAAATCATCGACCAGTTCGTGATGAAGCTGCGCATCGTCCGTTTC
ATCCGCACGCCTGATTACAATAACCTGTTCTCCGGAGACCCCACCTGGGTGACGGAATCCATCGGCGGCATGGGAGAGGA
CGGGCGCACCCTCGTCACCCGCAGTTCTTTCCGCATGCTGCAGACCCTGTACAACCTGGGGCCGGCTCCGGAACCGAACC
TGACCGTTCTGTGGTCCCGGAACCTGCCGGAAGCCTTCAAAAGCTTCTGCGCCAAGGTTTCCATTGAAACATCCTCCGTT
CAATACGAGAACGATGACCTGATGCGCCCACATTGGGGCGACGACTACGGCATCGCATGCTGCGTGTCCGCCATGCGCAT
CGGCAAGCAGATGCAGTTCTTCGGAGCGCGCGCCAACCTCGCCAAGTGCCTTCTCTACGCCCTGAACGGCGGCGTGGATG
AACTCAAGGGCAAGCAGGTGGCCCCGCCCTCCCCCCGCTACACGGAGGAAATTCTCAACTATGATGAAGTGATGACCCTG
TACGACAAGATGCAGGACTGGCTCGCCAAAACTTACATTGACGCGCTGAACATCATCCACTACATGCACGACAAATACTG
CTATGAACGCATTGAAATGGCGCTGCATGATCCGGAAATTCTGCGCACGATGGCTACGGGAATCGCCGGGCTTTCCGTGG
CGGCGGACTCCCTGTCCGCCATCAAGTATGCCACCGTAAAAGCCATCCGCAATGAAGAAGGGCTGATTGTGGACTTCAAG
ACGGAAGGGGAATTCCCCTGTTACGGGAACAATGACCCGCGCGTGGACGACATAGCATGCAGCCTGGTCAGCAATTTCAT
GGAAAAACTGCGCAGGCTCCACACTTACCGCAATTCCCTGCCCACCCAGTCCATCCTGACGATCACCTCCAACGTGGTGT
ACGGCAAAAAGACGGGCAACACGCCGGACGGCCGCCGAGCCGGGGAACCGTTCGCCCCCGGAGCCAACCCCATGCACGGA
AGGGACAGGAACGGAGCCGTGGCCTCCATGCTCTCCGTAGCCAAGCTGTCCTATGACGACTCCCTGGACGGCATCTCCTA
CACCTTCTCCATCGTTCCTCAGGCCCTGGGCAAGGAGGAACGTGAACGTCGCGTCAAGCTCGTCTCCCTGCTGGACGCCT
ACTTTGCCGCTACAGGTCACCACATTAATGTGAACGTACTGGAACGGGAAACGCTTCTCGACGCCATGGATCACCCGGAA
AAATACCCGCAGCTTACCATCCGCGTTTCCGGCTATGCCGTGAATTTCATCAAGCTGACCCGGGAACAGCAGCAGGAGGT
CATCAACCGCACTTTCCACACCCGTTAA

Upstream 100 bases:

>100_bases
GGAATTCACGATATAATTACGCGGCGCGGAGTTTTGCCGTTTCCAGCCTTTCAGGGGCTGCGGAACGTTTTTCTCCAGTC
TTCTTCACTACATTCTATTT

Downstream 100 bases:

>100_bases
CCCGGACTGCAAGCGGATGCCGCAGGTTTTTCCACAAAACCATGATCCGGACGGAGAAAGCTCCGTCACCGGTTTGGTTC
ATTCCGTGGAATCCTGCGGC

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 755; Mature: 755

Protein sequence:

>755_residues
MISIVKDLKESTALPQEWQGFKPGTWTESIDVRDFIQHNYTPYSGNEEFLSGPSQRTLRLWDELKVLLKREIDNGGVLDA
DEKVVSSITSHKPGYIDKELEVVVGLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYT
SDIRAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDKLIAERRKDLKNREHSPLTDELIRLREEMSEQIRALEELAQLG
ASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGRVSTFFDIYFTRDLEQGLITEEEVQEIIDQFVMKLRIVRF
IRTPDYNNLFSGDPTWVTESIGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSSV
QYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGKQVAPPSPRYTEEILNYDEVMTL
YDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHDPEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFK
TEGEFPCYGNNDPRVDDIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHG
RDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAYFAATGHHINVNVLERETLLDAMDHPE
KYPQLTIRVSGYAVNFIKLTREQQQEVINRTFHTR

Sequences:

>Translated_755_residues
MISIVKDLKESTALPQEWQGFKPGTWTESIDVRDFIQHNYTPYSGNEEFLSGPSQRTLRLWDELKVLLKREIDNGGVLDA
DEKVVSSITSHKPGYIDKELEVVVGLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYT
SDIRAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDKLIAERRKDLKNREHSPLTDELIRLREEMSEQIRALEELAQLG
ASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGRVSTFFDIYFTRDLEQGLITEEEVQEIIDQFVMKLRIVRF
IRTPDYNNLFSGDPTWVTESIGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSSV
QYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGKQVAPPSPRYTEEILNYDEVMTL
YDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHDPEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFK
TEGEFPCYGNNDPRVDDIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHG
RDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAYFAATGHHINVNVLERETLLDAMDHPE
KYPQLTIRVSGYAVNFIKLTREQQQEVINRTFHTR
>Mature_755_residues
MISIVKDLKESTALPQEWQGFKPGTWTESIDVRDFIQHNYTPYSGNEEFLSGPSQRTLRLWDELKVLLKREIDNGGVLDA
DEKVVSSITSHKPGYIDKELEVVVGLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYT
SDIRAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDKLIAERRKDLKNREHSPLTDELIRLREEMSEQIRALEELAQLG
ASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGRVSTFFDIYFTRDLEQGLITEEEVQEIIDQFVMKLRIVRF
IRTPDYNNLFSGDPTWVTESIGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSSV
QYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGKQVAPPSPRYTEEILNYDEVMTL
YDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHDPEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFK
TEGEFPCYGNNDPRVDDIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHG
RDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAYFAATGHHINVNVLERETLLDAMDHPE
KYPQLTIRVSGYAVNFIKLTREQQQEVINRTFHTR

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=752, Percent_Identity=63.031914893617, Blast_Score=991, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=745, Percent_Identity=62.0134228187919, Blast_Score=956, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=591, Percent_Identity=27.9187817258883, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1790388, Length=644, Percent_Identity=26.3975155279503, Blast_Score=152, Evalue=1e-37,
Organism=Escherichia coli, GI1788933, Length=54, Percent_Identity=75.9259259259259, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 85235; Mature: 85235

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISIVKDLKESTALPQEWQGFKPGTWTESIDVRDFIQHNYTPYSGNEEFLSGPSQRTLRL
CCHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHCCCCHHHHHH
WDELKVLLKREIDNGGVLDADEKVVSSITSHKPGYIDKELEVVVGLQTDAPLKRALMPFG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHCCCC
GLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYTSDIRAARSAGIITGLPDAYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCCCC
RGRIIGDYRRVALYGTDKLIAERRKDLKNREHSPLTDELIRLREEMSEQIRALEELAQLG
CCCEEECEEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGRVSTFFDIYFTRDLEQGLIT
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCHHHCCCC
EEEVQEIIDQFVMKLRIVRFIRTPDYNNLFSGDPTWVTESIGGMGEDGRTLVTRSSFRML
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEHHHHHHH
QTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSSVQYENDDLMRPHWGDDYGIAC
HHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHEEEECCCEEECCCCCCCCCCCCCHHHHH
CVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGKQVAPPSPRYTEEILNYDEVMTL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHCHHHHHHH
YDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHDPEILRTMATGIAGLSVAADSLSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVDDIACSLVSNFMEKLRRLHTYRNSL
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDRNGAVASMLSVAKLSYDD
CCHHHHHHHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
SLDGISYTFSIVPQALGKEERERRVKLVSLLDAYFAATGHHINVNVLERETLLDAMDHPE
CCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHCCCC
KYPQLTIRVSGYAVNFIKLTREQQQEVINRTFHTR
CCCEEEEEEECEEEEHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MISIVKDLKESTALPQEWQGFKPGTWTESIDVRDFIQHNYTPYSGNEEFLSGPSQRTLRL
CCHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHCCCCHHHHHH
WDELKVLLKREIDNGGVLDADEKVVSSITSHKPGYIDKELEVVVGLQTDAPLKRALMPFG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHCCCC
GLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYTSDIRAARSAGIITGLPDAYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEECCCCCCC
RGRIIGDYRRVALYGTDKLIAERRKDLKNREHSPLTDELIRLREEMSEQIRALEELAQLG
CCCEEECEEEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGRVSTFFDIYFTRDLEQGLIT
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCHHHCCCC
EEEVQEIIDQFVMKLRIVRFIRTPDYNNLFSGDPTWVTESIGGMGEDGRTLVTRSSFRML
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEHHHHHHH
QTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSSVQYENDDLMRPHWGDDYGIAC
HHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHEEEECCCEEECCCCCCCCCCCCCHHHHH
CVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGKQVAPPSPRYTEEILNYDEVMTL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHCHHHHHHH
YDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHDPEILRTMATGIAGLSVAADSLSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVDDIACSLVSNFMEKLRRLHTYRNSL
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDRNGAVASMLSVAKLSYDD
CCHHHHHHHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
SLDGISYTFSIVPQALGKEERERRVKLVSLLDAYFAATGHHINVNVLERETLLDAMDHPE
CCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHCCCC
KYPQLTIRVSGYAVNFIKLTREQQQEVINRTFHTR
CCCEEEEEEECEEEEHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA