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The map label for this gene is pflA [H]

Identifier: 187736032

GI number: 187736032

Start: 1853720

End: 1854499

Strand: Reverse

Name: pflA [H]

Synonym: Amuc_1542

Alternate gene names: 187736032

Gene position: 1854499-1853720 (Counterclockwise)

Preceding gene: 187736033

Following gene: 187736030

Centisome position: 69.61

GC content: 61.03

Gene sequence:

>780_bases
ATGCCGCAGGTTTTTCCACAAAACCATGATCCGGACGGAGAAAGCTCCGTCACCGGTTTGGTTCATTCCGTGGAATCCTG
CGGCACCGTGGACGGCCCCGGCATCCGCTTCGTCCTTTTCCTGTCCGGCTGCAGCCTGCGCTGCCGCTACTGCCACAATC
CGGATACCTCTTACGTGCGGCGGGGACGGACCAGGAGCGCAGATGATGTCCTCAAGGAAATAGCCCGTTACCGGGATTTT
TTGCAGGCGGCGGGCGGCGGCGTCACCCTCTCCGGCGGGGATCCCCTCTTCCAGCCGGATTTTGCCGGTGCCGTGCTGAA
AGGCTGCCGGAAACTGGGCCTGCACACCTGCCTGGATACCTCCGGCCATCTGGGCGTGAATGCGGGCGAAGAAATGCTGG
CGGATACGGACCTGGTCCTGCTGGATATCAAGGCATGGAATCCGAAACGGTATCGCGCCCTGACAGGCGGGGAACTGCGC
CCTACACTGGAGTTTGCGGAACGCCTGGCCTCCCTCCGCAAGCCCGTGTGGCTGAGGTATGTGCTGGTACCTGGCGTTAC
GGACAATCTGGAAGAAATTGAGGAACTCTCCCGCCATGCGGCGCTTCTGGGCAACGTGGAACGCGTGGACGTGCTCCCTT
TCCACCAGCTGGGGAGATTCAAATGGGATGAATTGAAGCTGGACTATACTTTGAAGGACGTCCCGGAAGCGTCCGCAGAA
CTGGCGGAACAGGTCCGCGGCATTTTCCGGCGGGGCGGCTTTCCGGATTGCGTGCATTAA

Upstream 100 bases:

>100_bases
GTTTCCGGCTATGCCGTGAATTTCATCAAGCTGACCCGGGAACAGCAGCAGGAGGTCATCAACCGCACTTTCCACACCCG
TTAACCCGGACTGCAAGCGG

Downstream 100 bases:

>100_bases
CCGGATTTTCGCGCTTTGGCGCCACCAACCGCAGCAAACCGGACGCAGGCATCCCCCTCTTCCGGATGAGGGGCCCTCTT
CCCTCCACTACAGAACCCAT

Product: pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCSLRCRYCHNPDTSYVRRGRTRSADDVLKEIARYRDF
LQAAGGGVTLSGGDPLFQPDFAGAVLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELR
PTLEFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKWDELKLDYTLKDVPEASAE
LAEQVRGIFRRGGFPDCVH

Sequences:

>Translated_259_residues
MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCSLRCRYCHNPDTSYVRRGRTRSADDVLKEIARYRDF
LQAAGGGVTLSGGDPLFQPDFAGAVLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELR
PTLEFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKWDELKLDYTLKDVPEASAE
LAEQVRGIFRRGGFPDCVH
>Mature_258_residues
PQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCSLRCRYCHNPDTSYVRRGRTRSADDVLKEIARYRDFL
QAAGGGVTLSGGDPLFQPDFAGAVLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRP
TLEFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKWDELKLDYTLKDVPEASAEL
AEQVRGIFRRGGFPDCVH

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=240, Percent_Identity=44.1666666666667, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1790389, Length=264, Percent_Identity=27.2727272727273, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790839, Length=285, Percent_Identity=29.1228070175439, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI226510931, Length=188, Percent_Identity=28.7234042553192, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 28672; Mature: 28541

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCSLRCRYCHNPDTSYVR
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCCCCHHHHH
RGRTRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGAVLKGCRKLGLHTCLDT
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
SGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTLEFAERLASLRKPVWLRY
CCCCCCCCCHHHHCCCCEEEEEEECCCCHHHEECCCCCCCHHHHHHHHHHHHCCCCEEEE
VLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKWDELKLDYTLKDVPEASAE
EEECCCCCCHHHHHHHHHHHHHHCCCHHEECCCHHHHCCCCHHHEEEEEEHHHCCHHHHH
LAEQVRGIFRRGGFPDCVH
HHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCSLRCRYCHNPDTSYVR
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCCCCHHHHH
RGRTRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGAVLKGCRKLGLHTCLDT
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
SGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTLEFAERLASLRKPVWLRY
CCCCCCCCCHHHHCCCCEEEEEEECCCCHHHEECCCCCCCHHHHHHHHHHHHCCCCEEEE
VLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKWDELKLDYTLKDVPEASAE
EEECCCCCCHHHHHHHHHHHHHHCCCHHEECCCHHHHCCCCHHHEEEEEEHHHCCHHHHH
LAEQVRGIFRRGGFPDCVH
HHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]