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The map label for this gene is hisH [H]

Identifier: 187735966

GI number: 187735966

Start: 1766333

End: 1766941

Strand: Direct

Name: hisH [H]

Synonym: Amuc_1475

Alternate gene names: 187735966

Gene position: 1766333-1766941 (Clockwise)

Preceding gene: 187735965

Following gene: 187735971

Centisome position: 66.3

GC content: 57.31

Gene sequence:

>609_bases
ATGAAACTTGGCGTGATTGATTACGGTGCGGGCAATCTCCGCAGTGTGCTGAACACCTTTGAGGCCGCCGGCGTGACCGG
TCATCTGGTGCGTACTCCGGAAGATGCGGCGGGCGTAACCCATTTGGTTCTTCCCGGCGTGGGCGCCTTCGGCGACTGTG
CAGAAAAGCTGCGCCGCCAGAATCTGGTGCCCCTGGTCCGGGCATGGATAGAGCGGGACAGGCCTTTTCTGGGTATCTGC
GTGGGCTATCAGGTTCTTTTTGAAAGCGGGGAAGAAAGCGGGCAGGTTCCCGGACTGGGCATCCTCAAGGGGCGCGTGGT
TCGATTTTCAGAATCGGAACTCAAGGTGCCTCACATGGGGTGGAACAGCGTGGCTCTCACTGATCCCGCCGATCCCATCT
GGAAAGGCATGGGGAAGGAACCGTACTTTTATTTCGTGCATTCCTATTATCCCCAGCCGGAGGATGAATCCCTGATTGCC
GCTTTCTGCACCTACGGCGTGCGTTTTGCTGCCGCTGTCAGGCACGGCAATCTGGTCGCCACCCAGTTCCACCCGGAAAA
AAGCCAGAAACTGGGCGTGAAACTGCTGAAAAACTTTATTTCCCTGTGA

Upstream 100 bases:

>100_bases
CTGTCTTCAAGGGCATCGCCCACGCGTTGCGGCAGGCTGCGGCGATTGATCCCCGAGCCGCCGGGACGCTGCCTTCAACC
AAGGGAATGCTGTAAAATAC

Downstream 100 bases:

>100_bases
GCCGGAAATTACCGGCTCACAGACTGCCGCTCTTTTTCCTGACAGCAAGGGGCAAGGAGGAGCGGTGAATCAAATGAAAC
GTCATTCTCGAAAAGCAGCC

Product: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MKLGVIDYGAGNLRSVLNTFEAAGVTGHLVRTPEDAAGVTHLVLPGVGAFGDCAEKLRRQNLVPLVRAWIERDRPFLGIC
VGYQVLFESGEESGQVPGLGILKGRVVRFSESELKVPHMGWNSVALTDPADPIWKGMGKEPYFYFVHSYYPQPEDESLIA
AFCTYGVRFAAAVRHGNLVATQFHPEKSQKLGVKLLKNFISL

Sequences:

>Translated_202_residues
MKLGVIDYGAGNLRSVLNTFEAAGVTGHLVRTPEDAAGVTHLVLPGVGAFGDCAEKLRRQNLVPLVRAWIERDRPFLGIC
VGYQVLFESGEESGQVPGLGILKGRVVRFSESELKVPHMGWNSVALTDPADPIWKGMGKEPYFYFVHSYYPQPEDESLIA
AFCTYGVRFAAAVRHGNLVATQFHPEKSQKLGVKLLKNFISL
>Mature_202_residues
MKLGVIDYGAGNLRSVLNTFEAAGVTGHLVRTPEDAAGVTHLVLPGVGAFGDCAEKLRRQNLVPLVRAWIERDRPFLGIC
VGYQVLFESGEESGQVPGLGILKGRVVRFSESELKVPHMGWNSVALTDPADPIWKGMGKEPYFYFVHSYYPQPEDESLIA
AFCTYGVRFAAAVRHGNLVATQFHPEKSQKLGVKLLKNFISL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=202, Percent_Identity=38.1188118811881, Blast_Score=128, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6319725, Length=207, Percent_Identity=37.6811594202899, Blast_Score=136, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22188; Mature: 22188

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGVIDYGAGNLRSVLNTFEAAGVTGHLVRTPEDAAGVTHLVLPGVGAFGDCAEKLRRQ
CCEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCHHHCCEEEECCCCCCHHHHHHHHHHC
NLVPLVRAWIERDRPFLGICVGYQVLFESGEESGQVPGLGILKGRVVRFSESELKVPHMG
CCHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCCEEEECCEEEEECCCCCCCCCCC
WNSVALTDPADPIWKGMGKEPYFYFVHSYYPQPEDESLIAAFCTYGVRFAAAVRHGNLVA
CCCEEECCCCCHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
TQFHPEKSQKLGVKLLKNFISL
EEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLGVIDYGAGNLRSVLNTFEAAGVTGHLVRTPEDAAGVTHLVLPGVGAFGDCAEKLRRQ
CCEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCHHHCCEEEECCCCCCHHHHHHHHHHC
NLVPLVRAWIERDRPFLGICVGYQVLFESGEESGQVPGLGILKGRVVRFSESELKVPHMG
CCHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCCCCEEEECCEEEEECCCCCCCCCCC
WNSVALTDPADPIWKGMGKEPYFYFVHSYYPQPEDESLIAAFCTYGVRFAAAVRHGNLVA
CCCEEECCCCCHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
TQFHPEKSQKLGVKLLKNFISL
EEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA