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The map label for this gene is rfbM [H]

Identifier: 187735686

GI number: 187735686

Start: 1427912

End: 1428970

Strand: Reverse

Name: rfbM [H]

Synonym: Amuc_1192

Alternate gene names: 187735686

Gene position: 1428970-1427912 (Counterclockwise)

Preceding gene: 187735687

Following gene: 187735685

Centisome position: 53.64

GC content: 58.45

Gene sequence:

>1059_bases
ATGAACCAATACGCTCTTATTCTGGCCGGCGGCAGCGGCACCCGTTTCTGGCCTCTTTCCCGCGACCACCGCCCCAAACA
ACTCCTGAACATGTTCGGAGAAGGAACCCTCCTTCGCCAGGCTATCGACAGGCTGGACGGGCTGGTGCCCAGGCAAAACA
TCTTTATCCTGACCAACCATCTGCAGGAAGCGGAAGTGCGCCGCCAGGCCCACGATATTCCCGTGGACAACATCATTTCC
GAACCCGTGCGCCGGGACACGGCGCCTGCCATCGCCCTGGGCATTGGTCTCATCAAGGCGGCGGACCCGCACGGAGTCAT
GCTCGTTATCCCCTCCGACCAGCTCATTCAGGACCAGGACTCCTTCCGCACCCTGATGAAAGCCGCCATGGACACGGCTG
CCAGGGAAAAAGCGCTTGTCACCGTGGGTATCAAGCCAACCTGGCCCTGCCCCTCCTACGGCTACATTGAACGCGGAAAA
GAAATCCACTCCGCTCCGGGCTGTTCCTGCCGCGAAGTCATTCAATTCCGCGAAAAACCGGACACGGCCACGGCTGCAGC
CTACCTGAGCCGGGGCAATTTCTGCTGGAACGCCGGCATGTTCGTATGGAGCATTCCTGCCGTGTGCGAACAGCTGGACA
AGCACTGCCCGGAACTGGCCTCCTTCGTGGAACACGTTGCGGAATCTCCGGCCCCGCAGGAAACCATCCGGGAAGAATTC
CCGGCGCTCACCCCCGTCTCCATAGACTTCGCGCTCATGGAGCATGCGGACAGAGTGCTGAACATGGAAGCAACCTTTGA
CTGGGACGACGTAGGTTCCTGGATATCCGTAGCCAACTATCTGGAAACCCACAATAAAAACACCACCAACACGGACATCA
CCGTGCAGGACGCCTCCGGCAACATTGTTTTCTCCCAAAAGGGGGACAAGCACGTGGCCCTGCTGGGAGTGGAAAACCTG
ATTGTGGTGGAAACGGCGGACGCCATCCTCATTGCGGATAAAAACAAGGCGGACGAAATCAAGAAAATCGTCAACCAGCT
TCCGGACGAACTCAGATGA

Upstream 100 bases:

>100_bases
GCGACCGGGACATGGAAGCTGCTCTGCTGGACTACTCCCGTAGAAAACGCCGTTTCGGCGCTCTCTGACCCTCTTGAACC
CCCATTCCTGCGAAACAGCC

Downstream 100 bases:

>100_bases
CCAGTCCGCACCCCGCCCTGGGCATCGACTACGGAGAAGCCCGCATCGGCATTGCCGCCACGGACCCCGTGGGCATCATG
GCCCATCCCGTAGAAACTAT

Product: Mannose-1-phosphate guanylyltransferase (GDP)

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP; GTP--mannose-1-phosphate guanylyltransferase [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MNQYALILAGGSGTRFWPLSRDHRPKQLLNMFGEGTLLRQAIDRLDGLVPRQNIFILTNHLQEAEVRRQAHDIPVDNIIS
EPVRRDTAPAIALGIGLIKAADPHGVMLVIPSDQLIQDQDSFRTLMKAAMDTAAREKALVTVGIKPTWPCPSYGYIERGK
EIHSAPGCSCREVIQFREKPDTATAAAYLSRGNFCWNAGMFVWSIPAVCEQLDKHCPELASFVEHVAESPAPQETIREEF
PALTPVSIDFALMEHADRVLNMEATFDWDDVGSWISVANYLETHNKNTTNTDITVQDASGNIVFSQKGDKHVALLGVENL
IVVETADAILIADKNKADEIKKIVNQLPDELR

Sequences:

>Translated_352_residues
MNQYALILAGGSGTRFWPLSRDHRPKQLLNMFGEGTLLRQAIDRLDGLVPRQNIFILTNHLQEAEVRRQAHDIPVDNIIS
EPVRRDTAPAIALGIGLIKAADPHGVMLVIPSDQLIQDQDSFRTLMKAAMDTAAREKALVTVGIKPTWPCPSYGYIERGK
EIHSAPGCSCREVIQFREKPDTATAAAYLSRGNFCWNAGMFVWSIPAVCEQLDKHCPELASFVEHVAESPAPQETIREEF
PALTPVSIDFALMEHADRVLNMEATFDWDDVGSWISVANYLETHNKNTTNTDITVQDASGNIVFSQKGDKHVALLGVENL
IVVETADAILIADKNKADEIKKIVNQLPDELR
>Mature_352_residues
MNQYALILAGGSGTRFWPLSRDHRPKQLLNMFGEGTLLRQAIDRLDGLVPRQNIFILTNHLQEAEVRRQAHDIPVDNIIS
EPVRRDTAPAIALGIGLIKAADPHGVMLVIPSDQLIQDQDSFRTLMKAAMDTAAREKALVTVGIKPTWPCPSYGYIERGK
EIHSAPGCSCREVIQFREKPDTATAAAYLSRGNFCWNAGMFVWSIPAVCEQLDKHCPELASFVEHVAESPAPQETIREEF
PALTPVSIDFALMEHADRVLNMEATFDWDDVGSWISVANYLETHNKNTTNTDITVQDASGNIVFSQKGDKHVALLGVENL
IVVETADAILIADKNKADEIKKIVNQLPDELR

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS group B O antigen [H]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=358, Percent_Identity=36.5921787709497, Blast_Score=195, Evalue=5e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 39058; Mature: 39058

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQYALILAGGSGTRFWPLSRDHRPKQLLNMFGEGTLLRQAIDRLDGLVPRQNIFILTNH
CCCEEEEEECCCCCEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECC
LQEAEVRRQAHDIPVDNIISEPVRRDTAPAIALGIGLIKAADPHGVMLVIPSDQLIQDQD
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCHHHCCHH
SFRTLMKAAMDTAAREKALVTVGIKPTWPCPSYGYIERGKEIHSAPGCSCREVIQFREKP
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHCCCHHCCCCCCCHHHHHHHHCCC
DTATAAAYLSRGNFCWNAGMFVWSIPAVCEQLDKHCPELASFVEHVAESPAPQETIREEF
CHHHHHHHHHCCCEEEECCHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
PALTPVSIDFALMEHADRVLNMEATFDWDDVGSWISVANYLETHNKNTTNTDITVQDASG
CCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
NIVFSQKGDKHVALLGVENLIVVETADAILIADKNKADEIKKIVNQLPDELR
CEEEECCCCCEEEEEECCCEEEEEECCEEEEECCCCHHHHHHHHHHCHHHCC
>Mature Secondary Structure
MNQYALILAGGSGTRFWPLSRDHRPKQLLNMFGEGTLLRQAIDRLDGLVPRQNIFILTNH
CCCEEEEEECCCCCEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECC
LQEAEVRRQAHDIPVDNIISEPVRRDTAPAIALGIGLIKAADPHGVMLVIPSDQLIQDQD
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCHHHCCHH
SFRTLMKAAMDTAAREKALVTVGIKPTWPCPSYGYIERGKEIHSAPGCSCREVIQFREKP
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHCCCHHCCCCCCCHHHHHHHHCCC
DTATAAAYLSRGNFCWNAGMFVWSIPAVCEQLDKHCPELASFVEHVAESPAPQETIREEF
CHHHHHHHHHCCCEEEECCHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHC
PALTPVSIDFALMEHADRVLNMEATFDWDDVGSWISVANYLETHNKNTTNTDITVQDASG
CCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
NIVFSQKGDKHVALLGVENLIVVETADAILIADKNKADEIKKIVNQLPDELR
CEEEECCCCCEEEEEECCCEEEEEECCEEEEECCCCHHHHHHHHHHCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]