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The map label for this gene is agaA [H]

Identifier: 187735681

GI number: 187735681

Start: 1423063

End: 1424664

Strand: Direct

Name: agaA [H]

Synonym: Amuc_1187

Alternate gene names: 187735681

Gene position: 1423063-1424664 (Clockwise)

Preceding gene: 187735680

Following gene: 187735684

Centisome position: 53.42

GC content: 56.49

Gene sequence:

>1602_bases
ATGAGATTGTACCATTTTTTACTCCCTGCCGTTGTCAGCGCTGCCGTATCTGCGTCATTTGGGGCAGAGTTCCCTAATCC
CTATCCTGCGCCCGCTCCCGGTGTCCGCCTGACTCCAGAGATTCCGCTTTCACCCTCCATTAATGGCGCCCGTATCGTCG
GGGCTACCCCCGGTTCCCGCATGCTGTTCCAGGTTCCCGTCTCCGGGGAGCGGCCCATGAAAATTCAGGCAACAGGGCTG
CCCCCAGGCCTGAAGATGGATTCGCGCGGATTGATTTCGGGTACCGCTCCGTCCGGGAAGAGGGAATACAAGGTAAATAT
CCAGGCTTCCAACAGGCATGGAAAGGACATGAAGGAGCTGATTCTGAAGGTGGGGGACGAATTGTGCCTGACTCCGCCCA
TGGGCTGGAGCAGCTGGTATTCCTACAGTGAGGCCGTAGGGGAGGATAATGTGCTGAAGACGGCACGGCTTTTTGTGGAA
CGGGGTCTGGTCAATCATGGCTGGGCCTATATCAACATTGACGACTGCTGGCAGGGCAGGCGCGGAGGGAAGTATGGCGC
CATTCAACCCAATAAGCGTTTTCCTGACATGAAGGCCATGTGCGACGCTATTCACGCCATGGGCATGAAAGCGGGCATTT
ATTCCACGCCTTGGATGGGAACGTATGCCGGTTTTATCGGAGGGAGCGCGCCCAACGCTAAGCCGGACTACGGGGAAATG
GCCATTCCGGAAAAGGAGCGCAAGCAGGAGGATCAAATCTTTGGAAGTTATCCGGGAGTTCATCGCAGAAAGGCGGATCA
TGTGGGAGCCGTCTGGCTGTTTGACCGTGACGCTAAACAATGGGCGGATTGGGGGTTCGATTATGTGAAAGTGGATTGGA
ATCCCAACGATGTGTCTACGACAAAGCGCATCCGCAAGGCGCTGGACGAGTCCGGGAGGGATATCGTGCTCAGCCTGTCC
AATGCCGCCCCGTACGAACATGTGGAAGAGCTGGGCAAGCTGGCGAATTTATGGCGGACGACGGGGGATATCCAGGATCA
CTGGGGCAGCGTCAGCGGCATCGGTTTTTCCCAGGAACGCTGGCAGAAGCATATGCGCCCGGGACATTGGAATGATCCGG
ACATCCTCCAGATCGGGAAGCTGGGCAAACCCAACCAGCCCAACACCACGTTTGTCCAGACGCGGCTGACTCCGGATGAA
CAGTACACCCATGTGACCCTGTGGTGCCTGCTGTCCGCTCCGCTCATCGTCTCCTGTGATTTGGAGCATATTGATTCGTT
TACGATGGGACTGCTTACCAATGATGAGGTGATAGCGGTGGATCAGGATCCGGCTGCCCGTCCCGCCCGCAAAGCGTGGC
ACCAGGGGAATTTCCAGGTGTGGATGAAGGAGTTGTCCGACGGTTCCGTGGCGGCTGGCTTTTTCAATACCGGGAAGGAG
AAAGGAATTTTGAAGGTGAATCTGAAGGAGCTGGGGCTTTCCGGAGCGTATGAGGCAAGGGACCTCTGGAAACGCGCTGA
CCAGGGGACCGTACAGGGAGATATGGCGGTAGAATTGAACGGGCATGGAGCATCCATGTTCCGGTTCAGCAAAAAGAAGT
AA

Upstream 100 bases:

>100_bases
CGCAGAAACAGGATGGACTGCGGAAGGAGGGAGGAATGGGGTCAATGAAAGGAATGACAAAGCAACTACGTTTTTAAGGT
ACACCTTTATAACCCTGTTT

Downstream 100 bases:

>100_bases
CGGGAAGGAGACGGCTCTGTATTCATTCAGGGGCGGCGGGGATATGCCGCCCCTGAAGCCTGTGGCAGGGGAGGCTTTTC
ATTGAACCGGCATCCATAGA

Product: Alpha-galactosidase

Products: NA

Alternate protein names: Alpha-D-galactoside galactohydrolase; Alpha-galactosidase 27A; Melibiase [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MRLYHFLLPAVVSAAVSASFGAEFPNPYPAPAPGVRLTPEIPLSPSINGARIVGATPGSRMLFQVPVSGERPMKIQATGL
PPGLKMDSRGLISGTAPSGKREYKVNIQASNRHGKDMKELILKVGDELCLTPPMGWSSWYSYSEAVGEDNVLKTARLFVE
RGLVNHGWAYINIDDCWQGRRGGKYGAIQPNKRFPDMKAMCDAIHAMGMKAGIYSTPWMGTYAGFIGGSAPNAKPDYGEM
AIPEKERKQEDQIFGSYPGVHRRKADHVGAVWLFDRDAKQWADWGFDYVKVDWNPNDVSTTKRIRKALDESGRDIVLSLS
NAAPYEHVEELGKLANLWRTTGDIQDHWGSVSGIGFSQERWQKHMRPGHWNDPDILQIGKLGKPNQPNTTFVQTRLTPDE
QYTHVTLWCLLSAPLIVSCDLEHIDSFTMGLLTNDEVIAVDQDPAARPARKAWHQGNFQVWMKELSDGSVAAGFFNTGKE
KGILKVNLKELGLSGAYEARDLWKRADQGTVQGDMAVELNGHGASMFRFSKKK

Sequences:

>Translated_533_residues
MRLYHFLLPAVVSAAVSASFGAEFPNPYPAPAPGVRLTPEIPLSPSINGARIVGATPGSRMLFQVPVSGERPMKIQATGL
PPGLKMDSRGLISGTAPSGKREYKVNIQASNRHGKDMKELILKVGDELCLTPPMGWSSWYSYSEAVGEDNVLKTARLFVE
RGLVNHGWAYINIDDCWQGRRGGKYGAIQPNKRFPDMKAMCDAIHAMGMKAGIYSTPWMGTYAGFIGGSAPNAKPDYGEM
AIPEKERKQEDQIFGSYPGVHRRKADHVGAVWLFDRDAKQWADWGFDYVKVDWNPNDVSTTKRIRKALDESGRDIVLSLS
NAAPYEHVEELGKLANLWRTTGDIQDHWGSVSGIGFSQERWQKHMRPGHWNDPDILQIGKLGKPNQPNTTFVQTRLTPDE
QYTHVTLWCLLSAPLIVSCDLEHIDSFTMGLLTNDEVIAVDQDPAARPARKAWHQGNFQVWMKELSDGSVAAGFFNTGKE
KGILKVNLKELGLSGAYEARDLWKRADQGTVQGDMAVELNGHGASMFRFSKKK
>Mature_533_residues
MRLYHFLLPAVVSAAVSASFGAEFPNPYPAPAPGVRLTPEIPLSPSINGARIVGATPGSRMLFQVPVSGERPMKIQATGL
PPGLKMDSRGLISGTAPSGKREYKVNIQASNRHGKDMKELILKVGDELCLTPPMGWSSWYSYSEAVGEDNVLKTARLFVE
RGLVNHGWAYINIDDCWQGRRGGKYGAIQPNKRFPDMKAMCDAIHAMGMKAGIYSTPWMGTYAGFIGGSAPNAKPDYGEM
AIPEKERKQEDQIFGSYPGVHRRKADHVGAVWLFDRDAKQWADWGFDYVKVDWNPNDVSTTKRIRKALDESGRDIVLSLS
NAAPYEHVEELGKLANLWRTTGDIQDHWGSVSGIGFSQERWQKHMRPGHWNDPDILQIGKLGKPNQPNTTFVQTRLTPDE
QYTHVTLWCLLSAPLIVSCDLEHIDSFTMGLLTNDEVIAVDQDPAARPARKAWHQGNFQVWMKELSDGSVAAGFFNTGKE
KGILKVNLKELGLSGAYEARDLWKRADQGTVQGDMAVELNGHGASMFRFSKKK

Specific function: Hydrolyzes galactomannan found in plant cell wall, by cleaving alpha-1,6-D-galactose side-chains from the mannan backbone. Appears to act in synergy with mannanase (ManA) to elicit hydrolysis of galactomannan. Has greater activity against galactomannans w

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 27 family [H]

Homologues:

Organism=Homo sapiens, GI4504009, Length=375, Percent_Identity=29.8666666666667, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI4557781, Length=414, Percent_Identity=28.5024154589372, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17562946, Length=452, Percent_Identity=28.7610619469027, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24583253, Length=441, Percent_Identity=30.6122448979592, Blast_Score=162, Evalue=7e-40,
Organism=Drosophila melanogaster, GI19922382, Length=452, Percent_Identity=27.8761061946903, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24654142, Length=452, Percent_Identity=27.8761061946903, Blast_Score=156, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013780
- InterPro:   IPR002241
- InterPro:   IPR017853
- InterPro:   IPR000111 [H]

Pfam domain/function: PF02065 Melibiase [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 59098; Mature: 59098

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLYHFLLPAVVSAAVSASFGAEFPNPYPAPAPGVRLTPEIPLSPSINGARIVGATPGSR
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEECCCCCC
MLFQVPVSGERPMKIQATGLPPGLKMDSRGLISGTAPSGKREYKVNIQASNRHGKDMKEL
EEEEECCCCCCCEEEEECCCCCCCCCCCCCCEECCCCCCCCEEEEEEEECCCCCCHHHHH
ILKVGDELCLTPPMGWSSWYSYSEAVGEDNVLKTARLFVERGLVNHGWAYINIDDCWQGR
HHHCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHCCEEEEEEHHHCCCC
RGGKYGAIQPNKRFPDMKAMCDAIHAMGMKAGIYSTPWMGTYAGFIGGSAPNAKPDYGEM
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCC
AIPEKERKQEDQIFGSYPGVHRRKADHVGAVWLFDRDAKQWADWGFDYVKVDWNPNDVST
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCHHHHHHCCCEEEEEECCCCCHHH
TKRIRKALDESGRDIVLSLSNAAPYEHVEELGKLANLWRTTGDIQDHWGSVSGIGFSQER
HHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHH
WQKHMRPGHWNDPDILQIGKLGKPNQPNTTFVQTRLTPDEQYTHVTLWCLLSAPLIVSCD
HHHHCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCHHEEEEEEECCCCEEEEEC
LEHIDSFTMGLLTNDEVIAVDQDPAARPARKAWHQGNFQVWMKELSDGSVAAGFFNTGKE
HHHHCCHHEEEECCCCEEEECCCCCCCHHHHHHHCCCHHHHHHHHCCCCEEEEECCCCCC
KGILKVNLKELGLSGAYEARDLWKRADQGTVQGDMAVELNGHGASMFRFSKKK
CCEEEEEHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEECCCC
>Mature Secondary Structure
MRLYHFLLPAVVSAAVSASFGAEFPNPYPAPAPGVRLTPEIPLSPSINGARIVGATPGSR
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEECCCCCC
MLFQVPVSGERPMKIQATGLPPGLKMDSRGLISGTAPSGKREYKVNIQASNRHGKDMKEL
EEEEECCCCCCCEEEEECCCCCCCCCCCCCCEECCCCCCCCEEEEEEEECCCCCCHHHHH
ILKVGDELCLTPPMGWSSWYSYSEAVGEDNVLKTARLFVERGLVNHGWAYINIDDCWQGR
HHHCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHCCEEEEEEHHHCCCC
RGGKYGAIQPNKRFPDMKAMCDAIHAMGMKAGIYSTPWMGTYAGFIGGSAPNAKPDYGEM
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCC
AIPEKERKQEDQIFGSYPGVHRRKADHVGAVWLFDRDAKQWADWGFDYVKVDWNPNDVST
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCHHHHHHCCCEEEEEECCCCCHHH
TKRIRKALDESGRDIVLSLSNAAPYEHVEELGKLANLWRTTGDIQDHWGSVSGIGFSQER
HHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHH
WQKHMRPGHWNDPDILQIGKLGKPNQPNTTFVQTRLTPDEQYTHVTLWCLLSAPLIVSCD
HHHHCCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCHHEEEEEEECCCCEEEEEC
LEHIDSFTMGLLTNDEVIAVDQDPAARPARKAWHQGNFQVWMKELSDGSVAAGFFNTGKE
HHHHCCHHEEEECCCCEEEECCCCCCCHHHHHHHCCCHHHHHHHHCCCCEEEEECCCCCC
KGILKVNLKELGLSGAYEARDLWKRADQGTVQGDMAVELNGHGASMFRFSKKK
CCEEEEEHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA