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The map label for this gene is tig

Identifier: 187735547

GI number: 187735547

Start: 1254796

End: 1256106

Strand: Reverse

Name: tig

Synonym: Amuc_1052

Alternate gene names: 187735547

Gene position: 1256106-1254796 (Counterclockwise)

Preceding gene: 187735553

Following gene: 187735546

Centisome position: 47.15

GC content: 54.08

Gene sequence:

>1311_bases
ATGGAAATCAACGTTGATATTCAACCGGACTGCACAGCCACGCTGAAAGCTTCCATCCCTGCAGAAACCACGGCTGCGCG
CCGCGCCTCCATTGTAGATTCCTATGCCGCCAAAGCCAAGCTTCCCGGCTTCCGCCCCGGCAAAACACCCAAGTCCATCA
TTGAAAAGCGCTTCAAGAAAGAAATGGAAGAAGAACTGCTGGACACCCTTTTTGAAACGGCCTGCTCCACCGCTCTGGAA
GAAAACCCCAAATTGAAAGTGCTCAGCTTCGGAAAGCCGGAACAATCTCTGGATGACCAGGGCAACTACACCGCTACCAG
CACGATGACCGTGGTTCCCGAATTTGAATTGCCGGAATACAAAGGCATCGAAGTCAAGGTTCCCTCTTCCGAAGTAACGG
AAGCAGACGTAGAGGAAGCTCTGAACTCCCTGGCTGAACAAATCGCGGAATTCACCCCTGTGGACCGCGCCGCCCAAAAA
GACGACGTGGCGATTATCGACTTCAAAACCACGTTGGACGGCAAGCCCGTAGCGGAAGCCGTAGGCAAACCCGTAGGCTT
CCTGGAAGGCCGTGACGGCCAGTGGATGAAGGTGGAAGACGATCAATTCCTGCCCGGCTTCGCCTCCGCCCTGGAAGGCC
TGAATGCCGGTGACAGCAAGGATATCACCGTCACCATTCCGGATACCTTCCCAATTACGGAACTGCGCGGCAAGGAACTC
GTATTCCATGCCACTGTGAAAGAAGTACGGGAAAAACAGCTTCCGGCCATGGATGATGCTTTTGCGGAAAAAGTTCTTCC
CGGCAAAAACCTGGAAGAATTGAAAACCGCCCTGAAGGAAAACCTGGCCCAGCGCAAAGCCATGCAAATTGATGAGGCCA
AGGCCGATCAGATCACGGAAAAGCTGGCGGACATGCTTGACTTCAACCTTCCGGAAGCCGTCGTAGAACGTGAAGTGTAC
GGCATCCTCCAGCAGAAAATGCAGCAGGCCATGTACAGCGGAAACGCCCCGGCGGACATGGATAAGTTTGTGGAGGAAGC
CCGGGAAGAAGCCAAACAGGAGGCCAAGCGCAACCTTAAGGTCTTCTTCATGCTTCAGGAAGTGGCGCAGGTGGAAAAAA
TTGCCGTCACGGAAATGGAGCTTTACAACGAAGTGGCCCGCCAGGCCCGCCAGCAGAAAAAGAACCTCAAATCCTACATT
CGCGAACTCCAGCGCGAAGGCCGCGTGCATGGCATCAGAATGAGCCTGCTGACAGCCAAGGTTCTGGACTTCCTGACAAA
AGAGGCCAAAGTAACGGTAGACGAGCAGTAA

Upstream 100 bases:

>100_bases
ATACCCTCTCATTAAAGTTATCGCCGCTCGTGTTTTGACCGCTGCCGCCATCCCGGAGCTGAAACAGAACCACTTTCAAT
TAACACTGACCGTATTATTT

Downstream 100 bases:

>100_bases
CCCCTCCTCTGCACAAAACACCTTACCAGGGGGAGTTGGCGCACACATGCCCGACTCCCCCGTTTTGCATCTTTCGGACG
GGTAACGTACATTGAAAAAA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MEINVDIQPDCTATLKASIPAETTAARRASIVDSYAAKAKLPGFRPGKTPKSIIEKRFKKEMEEELLDTLFETACSTALE
ENPKLKVLSFGKPEQSLDDQGNYTATSTMTVVPEFELPEYKGIEVKVPSSEVTEADVEEALNSLAEQIAEFTPVDRAAQK
DDVAIIDFKTTLDGKPVAEAVGKPVGFLEGRDGQWMKVEDDQFLPGFASALEGLNAGDSKDITVTIPDTFPITELRGKEL
VFHATVKEVREKQLPAMDDAFAEKVLPGKNLEELKTALKENLAQRKAMQIDEAKADQITEKLADMLDFNLPEAVVEREVY
GILQQKMQQAMYSGNAPADMDKFVEEAREEAKQEAKRNLKVFFMLQEVAQVEKIAVTEMELYNEVARQARQQKKNLKSYI
RELQREGRVHGIRMSLLTAKVLDFLTKEAKVTVDEQ

Sequences:

>Translated_436_residues
MEINVDIQPDCTATLKASIPAETTAARRASIVDSYAAKAKLPGFRPGKTPKSIIEKRFKKEMEEELLDTLFETACSTALE
ENPKLKVLSFGKPEQSLDDQGNYTATSTMTVVPEFELPEYKGIEVKVPSSEVTEADVEEALNSLAEQIAEFTPVDRAAQK
DDVAIIDFKTTLDGKPVAEAVGKPVGFLEGRDGQWMKVEDDQFLPGFASALEGLNAGDSKDITVTIPDTFPITELRGKEL
VFHATVKEVREKQLPAMDDAFAEKVLPGKNLEELKTALKENLAQRKAMQIDEAKADQITEKLADMLDFNLPEAVVEREVY
GILQQKMQQAMYSGNAPADMDKFVEEAREEAKQEAKRNLKVFFMLQEVAQVEKIAVTEMELYNEVARQARQQKKNLKSYI
RELQREGRVHGIRMSLLTAKVLDFLTKEAKVTVDEQ
>Mature_436_residues
MEINVDIQPDCTATLKASIPAETTAARRASIVDSYAAKAKLPGFRPGKTPKSIIEKRFKKEMEEELLDTLFETACSTALE
ENPKLKVLSFGKPEQSLDDQGNYTATSTMTVVPEFELPEYKGIEVKVPSSEVTEADVEEALNSLAEQIAEFTPVDRAAQK
DDVAIIDFKTTLDGKPVAEAVGKPVGFLEGRDGQWMKVEDDQFLPGFASALEGLNAGDSKDITVTIPDTFPITELRGKEL
VFHATVKEVREKQLPAMDDAFAEKVLPGKNLEELKTALKENLAQRKAMQIDEAKADQITEKLADMLDFNLPEAVVEREVY
GILQQKMQQAMYSGNAPADMDKFVEEAREEAKQEAKRNLKVFFMLQEVAQVEKIAVTEMELYNEVARQARQQKKNLKSYI
RELQREGRVHGIRMSLLTAKVLDFLTKEAKVTVDEQ

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=384, Percent_Identity=26.3020833333333, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_AKKM8 (B2UQZ3)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877659.1
- GeneID:   6274058
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_1052
- OMA:   RFIPGFE
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 48713; Mature: 48713

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEINVDIQPDCTATLKASIPAETTAARRASIVDSYAAKAKLPGFRPGKTPKSIIEKRFKK
CEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
EMEEELLDTLFETACSTALEENPKLKVLSFGKPEQSLDDQGNYTATSTMTVVPEFELPEY
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEEEEEECCCCCCC
KGIEVKVPSSEVTEADVEEALNSLAEQIAEFTPVDRAAQKDDVAIIDFKTTLDGKPVAEA
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCEEEEEEECCCCCCHHHHH
VGKPVGFLEGRDGQWMKVEDDQFLPGFASALEGLNAGDSKDITVTIPDTFPITELRGKEL
CCCCCCCEECCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCHHCCCCCE
VFHATVKEVREKQLPAMDDAFAEKVLPGKNLEELKTALKENLAQRKAMQIDEAKADQITE
EHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLADMLDFNLPEAVVEREVYGILQQKMQQAMYSGNAPADMDKFVEEAREEAKQEAKRNLK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHH
VFFMLQEVAQVEKIAVTEMELYNEVARQARQQKKNLKSYIRELQREGRVHGIRMSLLTAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VLDFLTKEAKVTVDEQ
HHHHHHHCCCEEECCC
>Mature Secondary Structure
MEINVDIQPDCTATLKASIPAETTAARRASIVDSYAAKAKLPGFRPGKTPKSIIEKRFKK
CEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
EMEEELLDTLFETACSTALEENPKLKVLSFGKPEQSLDDQGNYTATSTMTVVPEFELPEY
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEEEEEEECCCCCCC
KGIEVKVPSSEVTEADVEEALNSLAEQIAEFTPVDRAAQKDDVAIIDFKTTLDGKPVAEA
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCEEEEEEECCCCCCHHHHH
VGKPVGFLEGRDGQWMKVEDDQFLPGFASALEGLNAGDSKDITVTIPDTFPITELRGKEL
CCCCCCCEECCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCHHCCCCCE
VFHATVKEVREKQLPAMDDAFAEKVLPGKNLEELKTALKENLAQRKAMQIDEAKADQITE
EHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLADMLDFNLPEAVVEREVYGILQQKMQQAMYSGNAPADMDKFVEEAREEAKQEAKRNLK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHH
VFFMLQEVAQVEKIAVTEMELYNEVARQARQQKKNLKSYIRELQREGRVHGIRMSLLTAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VLDFLTKEAKVTVDEQ
HHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA