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The map label for this gene is lepA [H]

Identifier: 187735531

GI number: 187735531

Start: 1229683

End: 1231482

Strand: Reverse

Name: lepA [H]

Synonym: Amuc_1036

Alternate gene names: 187735531

Gene position: 1231482-1229683 (Counterclockwise)

Preceding gene: 187735533

Following gene: 187735530

Centisome position: 46.23

GC content: 55.06

Gene sequence:

>1800_bases
ATGTCCATTGAACTGACTCGCAATTTCTCCATTATCGCTCACATCGACCACGGAAAGACCACCCTTTCCGACCGGCTGCT
GGAAAAGACGAATACCATTTCCGAACGGGAGAAGCAGGATCAGCTTCTGGACGCCATGGACCTGGAGCGGGAAAAGGGCA
TCACCATCAAGTCCCACCCCGTTACCATTTTCTACAAGGCCGGGGACGGAAAGACTTACAAGCTCAATCTGCTGGATACG
CCGGGCCATGTGGATTTCTCCTATGAAGTGTCCCGTTCCCTGGCGGCATGCGAAGGAGCCCTCCTTATTGTGGACGCAGC
CCAGGGAGTGGAAGCCCAGACACTTGCCAACATGCACCTGGCCATGGATCTGAACCTGGCCATTATCCCCGTCATCAATA
AGATTGACCTTCCCAGCGCCAACCTGCCCAAAGTGTACCGCCAGCTGGAGGACATCGTCTGCATCCCCCATGAGGAAGCC
ATCCATGCCTCCGCCAAGATGGGCATCGGCATTGACGATATTCTGGAAGCCGTTGTTCAGCGCATCCCTCCGCCTGAAAC
GGAACAGGACGGCCTTCTGCGCGCCCTGGTGTTCGATTCCGTTTACGACGCCTACCGCGGTGTAGTCTCCTATGTGCGCG
TCATTTCCGGCAGCGTGCACCGCGGCATGAAAGTCAAACTTTTCGCAACGGACGAAGTGTATGAAGTGAAGGAAGTGGGT
ATCTTCACTCCCAAAATGACCCGAACGGATTCCCTGGAAGCCGGTGACGTAGGCTACATCATCGCCAACATGAAATCCGC
GGCTGATGTCAAGATCGGGGACACGTATACGGATTACATGCGCCCCTGCCCATCCCCCCTGCCCGGTTTCAAGGAAATCC
GCCCCATGGTCTTTTCCGGCATTTATCCGGTGGATTCCTCCGATTTTGAAGCCCTGAAAGCGGCCATGGCGAAGCTTCAG
ATTAATGACGCCGCTTTCTCCTTCCAGGCGGAATCTTCCGTAGCTCTGGGCTTCGGGTTCCGTTGCGGCTTCCTTGGCCT
GCTCCATATGGAAATTATCCAAGAGCGTCTGCGCCGGGAATTCAACATGGATATCATCTCCACCTACCCCTCCGTGATTT
ATGAGGTCACCAAGACCAATGGAGAGGAAATCAATGTGGATAATCCCAGCCTGCTCCCGGAACCGCAGGAAATTCAGGAA
ATCCGGGAACCTATCGTCAAGGTGTTCATTATGCTTCCTGGGGAATACATCGGGGATATCATGCAACTTGTCCTGGAAAA
ACGCGGCAGCGTGGACAATACGGAGACCATTGACGACATGCGCGTAATGCTCACCTGCACCGTCCCTCTGGCGGAAATTC
TGGTGGATTTCAACGACAAGCTCAAATCCATGACGCGCGGCTACGGCTCCATGGATTATGAATACGCCGGCTACCAGGCC
GCCAAGCTCATTAAAATGGACATGCTCATCGCCGGGGAACCGGTGGATGCTTTCTCCATGATCGTTCACCAGGACAAGGC
AGCCTCCCGCGGCAGGGAACTGGCGGAACGCCTGAAAAACGTCATTCCCCGCCAGCTCTTCACCGTCGCCATTCAGGCCT
GCATTGGCGGAAAGATCATTGCCCGGGAAAGCATCTCCCCCATGCGCAAGGACGTGACGGCCAAATGCTACGGCGGGGAC
GTCACCCGCAAGCGCAAACTTCTGGAAAAGCAGAAGGAGGGCAAAAAGCGCATGAAGGCCATCGGAAAAATCAACATTCC
GCAGGAAGCGTTCATCAAGGTGCTGAAAACGGGGGATTAA

Upstream 100 bases:

>100_bases
CAGGGAACGGGAGGCAGTCATGTTCAAGTCTTGACCGCCGCATGGGCTATGTTACCCTCCCCGCGCACAGGGCACTCATG
TTAGAAGGGTAGAGACCACC

Downstream 100 bases:

>100_bases
CCCATTTTCTTCCCTCAAGGCATCCAAACGGGGTCTTCTGTTTCCTTCCCGGATTTATACATTTTTCCGTTTGAATATGG
GCCATGAAACACCTCCTGCA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MSIELTRNFSIIAHIDHGKTTLSDRLLEKTNTISEREKQDQLLDAMDLEREKGITIKSHPVTIFYKAGDGKTYKLNLLDT
PGHVDFSYEVSRSLAACEGALLIVDAAQGVEAQTLANMHLAMDLNLAIIPVINKIDLPSANLPKVYRQLEDIVCIPHEEA
IHASAKMGIGIDDILEAVVQRIPPPETEQDGLLRALVFDSVYDAYRGVVSYVRVISGSVHRGMKVKLFATDEVYEVKEVG
IFTPKMTRTDSLEAGDVGYIIANMKSAADVKIGDTYTDYMRPCPSPLPGFKEIRPMVFSGIYPVDSSDFEALKAAMAKLQ
INDAAFSFQAESSVALGFGFRCGFLGLLHMEIIQERLRREFNMDIISTYPSVIYEVTKTNGEEINVDNPSLLPEPQEIQE
IREPIVKVFIMLPGEYIGDIMQLVLEKRGSVDNTETIDDMRVMLTCTVPLAEILVDFNDKLKSMTRGYGSMDYEYAGYQA
AKLIKMDMLIAGEPVDAFSMIVHQDKAASRGRELAERLKNVIPRQLFTVAIQACIGGKIIARESISPMRKDVTAKCYGGD
VTRKRKLLEKQKEGKKRMKAIGKINIPQEAFIKVLKTGD

Sequences:

>Translated_599_residues
MSIELTRNFSIIAHIDHGKTTLSDRLLEKTNTISEREKQDQLLDAMDLEREKGITIKSHPVTIFYKAGDGKTYKLNLLDT
PGHVDFSYEVSRSLAACEGALLIVDAAQGVEAQTLANMHLAMDLNLAIIPVINKIDLPSANLPKVYRQLEDIVCIPHEEA
IHASAKMGIGIDDILEAVVQRIPPPETEQDGLLRALVFDSVYDAYRGVVSYVRVISGSVHRGMKVKLFATDEVYEVKEVG
IFTPKMTRTDSLEAGDVGYIIANMKSAADVKIGDTYTDYMRPCPSPLPGFKEIRPMVFSGIYPVDSSDFEALKAAMAKLQ
INDAAFSFQAESSVALGFGFRCGFLGLLHMEIIQERLRREFNMDIISTYPSVIYEVTKTNGEEINVDNPSLLPEPQEIQE
IREPIVKVFIMLPGEYIGDIMQLVLEKRGSVDNTETIDDMRVMLTCTVPLAEILVDFNDKLKSMTRGYGSMDYEYAGYQA
AKLIKMDMLIAGEPVDAFSMIVHQDKAASRGRELAERLKNVIPRQLFTVAIQACIGGKIIARESISPMRKDVTAKCYGGD
VTRKRKLLEKQKEGKKRMKAIGKINIPQEAFIKVLKTGD
>Mature_598_residues
SIELTRNFSIIAHIDHGKTTLSDRLLEKTNTISEREKQDQLLDAMDLEREKGITIKSHPVTIFYKAGDGKTYKLNLLDTP
GHVDFSYEVSRSLAACEGALLIVDAAQGVEAQTLANMHLAMDLNLAIIPVINKIDLPSANLPKVYRQLEDIVCIPHEEAI
HASAKMGIGIDDILEAVVQRIPPPETEQDGLLRALVFDSVYDAYRGVVSYVRVISGSVHRGMKVKLFATDEVYEVKEVGI
FTPKMTRTDSLEAGDVGYIIANMKSAADVKIGDTYTDYMRPCPSPLPGFKEIRPMVFSGIYPVDSSDFEALKAAMAKLQI
NDAAFSFQAESSVALGFGFRCGFLGLLHMEIIQERLRREFNMDIISTYPSVIYEVTKTNGEEINVDNPSLLPEPQEIQEI
REPIVKVFIMLPGEYIGDIMQLVLEKRGSVDNTETIDDMRVMLTCTVPLAEILVDFNDKLKSMTRGYGSMDYEYAGYQAA
KLIKMDMLIAGEPVDAFSMIVHQDKAASRGRELAERLKNVIPRQLFTVAIQACIGGKIIARESISPMRKDVTAKCYGGDV
TRKRKLLEKQKEGKKRMKAIGKINIPQEAFIKVLKTGD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=603, Percent_Identity=50.4145936981758, Blast_Score=629, Evalue=1e-180,
Organism=Homo sapiens, GI94966754, Length=131, Percent_Identity=43.5114503816794, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI217272894, Length=135, Percent_Identity=42.962962962963, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI217272892, Length=135, Percent_Identity=42.962962962963, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI4503483, Length=142, Percent_Identity=39.4366197183099, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI18390331, Length=184, Percent_Identity=34.2391304347826, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI25306283, Length=139, Percent_Identity=44.6043165467626, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI19923640, Length=139, Percent_Identity=44.6043165467626, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI25306287, Length=139, Percent_Identity=44.6043165467626, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI310132016, Length=108, Percent_Identity=39.8148148148148, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI310110807, Length=108, Percent_Identity=39.8148148148148, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI310123363, Length=108, Percent_Identity=39.8148148148148, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI53729339, Length=254, Percent_Identity=29.1338582677165, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI53729337, Length=254, Percent_Identity=29.1338582677165, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI94966752, Length=64, Percent_Identity=50, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788922, Length=592, Percent_Identity=54.0540540540541, Blast_Score=659, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=506, Percent_Identity=29.0513833992095, Blast_Score=163, Evalue=4e-41,
Organism=Escherichia coli, GI1789738, Length=150, Percent_Identity=39.3333333333333, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789559, Length=220, Percent_Identity=29.5454545454545, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1790835, Length=161, Percent_Identity=34.1614906832298, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=609, Percent_Identity=41.871921182266, Blast_Score=499, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17556745, Length=139, Percent_Identity=38.8489208633094, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=145, Percent_Identity=40, Blast_Score=97, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17552882, Length=134, Percent_Identity=39.5522388059701, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=154, Percent_Identity=35.7142857142857, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=38.0597014925373, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=38.0597014925373, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71994658, Length=211, Percent_Identity=27.0142180094787, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=44.5182724252492, Blast_Score=517, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6323098, Length=182, Percent_Identity=38.4615384615385, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6324707, Length=142, Percent_Identity=38.7323943661972, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=142, Percent_Identity=38.7323943661972, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=111, Percent_Identity=42.3423423423423, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=168, Percent_Identity=36.9047619047619, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI78706572, Length=604, Percent_Identity=44.3708609271523, Blast_Score=559, Evalue=1e-159,
Organism=Drosophila melanogaster, GI24582462, Length=153, Percent_Identity=39.2156862745098, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI221458488, Length=148, Percent_Identity=40.5405405405405, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI21357743, Length=134, Percent_Identity=39.5522388059701, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28574573, Length=135, Percent_Identity=41.4814814814815, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585709, Length=146, Percent_Identity=35.6164383561644, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24585711, Length=146, Percent_Identity=35.6164383561644, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24585713, Length=146, Percent_Identity=35.6164383561644, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28572034, Length=220, Percent_Identity=30.9090909090909, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66718; Mature: 66587

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIELTRNFSIIAHIDHGKTTLSDRLLEKTNTISEREKQDQLLDAMDLEREKGITIKSHP
CCEEEEECEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCC
VTIFYKAGDGKTYKLNLLDTPGHVDFSYEVSRSLAACEGALLIVDAAQGVEAQTLANMHL
EEEEEECCCCCEEEEEEECCCCCCEEEHHHHHHHHHHCCCEEEEECCCCCCHHHHHHEEE
AMDLNLAIIPVINKIDLPSANLPKVYRQLEDIVCIPHEEAIHASAKMGIGIDDILEAVVQ
EEECCEEEEEEECCCCCCCCCHHHHHHHHHHEEECCCHHHHHHHHHCCCCHHHHHHHHHH
RIPPPETEQDGLLRALVFDSVYDAYRGVVSYVRVISGSVHRGMKVKLFATDEVYEVKEVG
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCEEEEEEECCCHHHHHHCC
IFTPKMTRTDSLEAGDVGYIIANMKSAADVKIGDTYTDYMRPCPSPLPGFKEIRPMVFSG
EECCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCHHHHHCCCCCCCCCHHHHHHHHHHC
IYPVDSSDFEALKAAMAKLQINDAAFSFQAESSVALGFGFRCGFLGLLHMEIIQERLRRE
CCCCCCCHHHHHHHHHHHHEECCHHEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
FNMDIISTYPSVIYEVTKTNGEEINVDNPSLLPEPQEIQEIREPIVKVFIMLPGEYIGDI
HCCHHHHHHHHHHEEHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
MQLVLEKRGSVDNTETIDDMRVMLTCTVPLAEILVDFNDKLKSMTRGYGSMDYEYAGYQA
HHHHHHHCCCCCCCCHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
AKLIKMDMLIAGEPVDAFSMIVHQDKAASRGRELAERLKNVIPRQLFTVAIQACIGGKII
HHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
ARESISPMRKDVTAKCYGGDVTRKRKLLEKQKEGKKRMKAIGKINIPQEAFIKVLKTGD
ECCCCCHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SIELTRNFSIIAHIDHGKTTLSDRLLEKTNTISEREKQDQLLDAMDLEREKGITIKSHP
CEEEEECEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCC
VTIFYKAGDGKTYKLNLLDTPGHVDFSYEVSRSLAACEGALLIVDAAQGVEAQTLANMHL
EEEEEECCCCCEEEEEEECCCCCCEEEHHHHHHHHHHCCCEEEEECCCCCCHHHHHHEEE
AMDLNLAIIPVINKIDLPSANLPKVYRQLEDIVCIPHEEAIHASAKMGIGIDDILEAVVQ
EEECCEEEEEEECCCCCCCCCHHHHHHHHHHEEECCCHHHHHHHHHCCCCHHHHHHHHHH
RIPPPETEQDGLLRALVFDSVYDAYRGVVSYVRVISGSVHRGMKVKLFATDEVYEVKEVG
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCEEEEEEECCCHHHHHHCC
IFTPKMTRTDSLEAGDVGYIIANMKSAADVKIGDTYTDYMRPCPSPLPGFKEIRPMVFSG
EECCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCHHHHHCCCCCCCCCHHHHHHHHHHC
IYPVDSSDFEALKAAMAKLQINDAAFSFQAESSVALGFGFRCGFLGLLHMEIIQERLRRE
CCCCCCCHHHHHHHHHHHHEECCHHEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
FNMDIISTYPSVIYEVTKTNGEEINVDNPSLLPEPQEIQEIREPIVKVFIMLPGEYIGDI
HCCHHHHHHHHHHEEHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
MQLVLEKRGSVDNTETIDDMRVMLTCTVPLAEILVDFNDKLKSMTRGYGSMDYEYAGYQA
HHHHHHHCCCCCCCCHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
AKLIKMDMLIAGEPVDAFSMIVHQDKAASRGRELAERLKNVIPRQLFTVAIQACIGGKII
HHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
ARESISPMRKDVTAKCYGGDVTRKRKLLEKQKEGKKRMKAIGKINIPQEAFIKVLKTGD
ECCCCCHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA