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The map label for this gene is hemD [H]

Identifier: 187735398

GI number: 187735398

Start: 1068430

End: 1069950

Strand: Direct

Name: hemD [H]

Synonym: Amuc_0896

Alternate gene names: 187735398

Gene position: 1068430-1069950 (Clockwise)

Preceding gene: 187735395

Following gene: 187735400

Centisome position: 40.1

GC content: 61.6

Gene sequence:

>1521_bases
ATGCAACAGGGTATTGTCTATTTGCTTGGAGCGGGTCCCGGCGACCCCGGCCTGATAACCGTCCGCGGGCGGGAACTGGT
GGGAATGGCGGAAGTGCTGGTATATGATGCGCTGAGTTCGGCGGAAATGCTGAACTGGGCTCCTGCTGCATGCGAAAGGA
TTTTTGTGGGCAAGCGGGCTTCCCGCCACGCCCTGCCGCAGGAGGAAATCAACGCCCTGCTGGTCAGGCTGGGCCGTGCC
GGGAAAAAAGTGGTGCGCCTGAAAGGGGGCGACCCCTACGTCTTCGGGCGCGGGGGGGAGGAGGCGGACGCCCTGCATGA
AGCGGGCATCCCTTTTGAAGTTGTTCCGGGCGTTACTTCCGCCATTGCGGGGCCTGCCTACGCAGGCATTCCCGTGACGC
ACCGCGGGTACTGCACGCAGTTTACCGTATTTACGGGCCATGAAGGCGAGAACAAGAAGGCGTCTTCCCTGGATTTGAGG
GGAATAGCCGAGGCGCAGGGCACGAAAGTCATGCTGATGGGAATGCGGAAACTGGCGGATGTATGCGAAGCCCTGATCGG
GTACGGGCAGGAACCGTCTGTGCCTGCCGCCGCCGTCCAGTGGGCCACCACGGGCATTCAGCGCACGGTTACGGGAACTG
TGAAAACACTGCCCGCCAGGGTGCAGAAGGCCGGGTTGGGCGCTCCCGCAGTAGTTGTCATCGGGGATGTGGTGAAAGAA
CGCGAGTCCCTGAACTGGTTTGAAAAACTGCCCCTGTTCGGCAAGCGCATCGTGGTGACGCGCACCCGTGCCCAGGCCGG
GGAATTGAGCGCGCGCCTGCGCCGCCTGGGGGCGGAAGTGCTGGAGATGCCTGTCATACGCATTGCCCCGCCCTCCAACA
GGAGGGAATTTGCGGAAAGCGTGGTGCATGCCCACACATATGACTGGCTGGTGTTTTCCAGCCCCAACGGGGTGGAACGC
TTCTTCCAGGCGTTTTTTGCCGTGTACAGGGACATCCGGAGCATTGGAGGAGCCAGGATTGCGGCCATCGGGCCAGGGAC
GGAGGCGAAGCTCCGGGAATACGGGCTGGCCGTGGATGTCATGCCTAAAAAGTTTGTGGCGGAGGGCCTCGTTAAAGCGT
TCAGGGACGCACGCGAAGAAATAGGAACCATTGAACACAGCACATTCCTGTGGGTGCGCGGAGAAGAAGCGCGCCGCGTG
ATTTACGACGGGTTGAATGCGCTGGGCGCCATTGTGGACGAATGCATCGCCTACAAAACGGAAGCGGAAACGGAGGATGT
GGCGGGAGCCCAGGCGGCTTTCCGCGAACGCGGAGCGGACATCGTGACGTTCACCAGTTCCTCCACGGCGGAGAACTTTT
TCAAGCTGGGGCTTCCGTGGCCGGAGGGATGCAGGGCCGCCAGCATCGGCCCGGTAACCACGGCAACCCTCAAGGAGCTG
GGGCATGCCCCTTCAATTACAGCGAAAACACACGATATCAACGGTCTGGTGGAAGCCATCGTCAAGGCCGCCGGCAAATA
G

Upstream 100 bases:

>100_bases
TTTGCGGATGTTTTGTGACAATTTAGCCATCAATGAAATGAATCCGGGCATGGCGGGGTTTCTTGTCGCCAAAAAGCGGA
TGGTGGAGTTGAATGGCGGC

Downstream 100 bases:

>100_bases
GGAAAACGGCGCGGACGGCGTGAAACGCCGGGCCGGGCAGATGGGAAATACAAGGCGGGGAACGCCGTTTAAAAGCCATT
GACGGCTTCCCAGCCGATGA

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MQQGIVYLLGAGPGDPGLITVRGRELVGMAEVLVYDALSSAEMLNWAPAACERIFVGKRASRHALPQEEINALLVRLGRA
GKKVVRLKGGDPYVFGRGGEEADALHEAGIPFEVVPGVTSAIAGPAYAGIPVTHRGYCTQFTVFTGHEGENKKASSLDLR
GIAEAQGTKVMLMGMRKLADVCEALIGYGQEPSVPAAAVQWATTGIQRTVTGTVKTLPARVQKAGLGAPAVVVIGDVVKE
RESLNWFEKLPLFGKRIVVTRTRAQAGELSARLRRLGAEVLEMPVIRIAPPSNRREFAESVVHAHTYDWLVFSSPNGVER
FFQAFFAVYRDIRSIGGARIAAIGPGTEAKLREYGLAVDVMPKKFVAEGLVKAFRDAREEIGTIEHSTFLWVRGEEARRV
IYDGLNALGAIVDECIAYKTEAETEDVAGAQAAFRERGADIVTFTSSSTAENFFKLGLPWPEGCRAASIGPVTTATLKEL
GHAPSITAKTHDINGLVEAIVKAAGK

Sequences:

>Translated_506_residues
MQQGIVYLLGAGPGDPGLITVRGRELVGMAEVLVYDALSSAEMLNWAPAACERIFVGKRASRHALPQEEINALLVRLGRA
GKKVVRLKGGDPYVFGRGGEEADALHEAGIPFEVVPGVTSAIAGPAYAGIPVTHRGYCTQFTVFTGHEGENKKASSLDLR
GIAEAQGTKVMLMGMRKLADVCEALIGYGQEPSVPAAAVQWATTGIQRTVTGTVKTLPARVQKAGLGAPAVVVIGDVVKE
RESLNWFEKLPLFGKRIVVTRTRAQAGELSARLRRLGAEVLEMPVIRIAPPSNRREFAESVVHAHTYDWLVFSSPNGVER
FFQAFFAVYRDIRSIGGARIAAIGPGTEAKLREYGLAVDVMPKKFVAEGLVKAFRDAREEIGTIEHSTFLWVRGEEARRV
IYDGLNALGAIVDECIAYKTEAETEDVAGAQAAFRERGADIVTFTSSSTAENFFKLGLPWPEGCRAASIGPVTTATLKEL
GHAPSITAKTHDINGLVEAIVKAAGK
>Mature_506_residues
MQQGIVYLLGAGPGDPGLITVRGRELVGMAEVLVYDALSSAEMLNWAPAACERIFVGKRASRHALPQEEINALLVRLGRA
GKKVVRLKGGDPYVFGRGGEEADALHEAGIPFEVVPGVTSAIAGPAYAGIPVTHRGYCTQFTVFTGHEGENKKASSLDLR
GIAEAQGTKVMLMGMRKLADVCEALIGYGQEPSVPAAAVQWATTGIQRTVTGTVKTLPARVQKAGLGAPAVVVIGDVVKE
RESLNWFEKLPLFGKRIVVTRTRAQAGELSARLRRLGAEVLEMPVIRIAPPSNRREFAESVVHAHTYDWLVFSSPNGVER
FFQAFFAVYRDIRSIGGARIAAIGPGTEAKLREYGLAVDVMPKKFVAEGLVKAFRDAREEIGTIEHSTFLWVRGEEARRV
IYDGLNALGAIVDECIAYKTEAETEDVAGAQAAFRERGADIVTFTSSSTAENFFKLGLPWPEGCRAASIGPVTTATLKEL
GHAPSITAKTHDINGLVEAIVKAAGK

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=248, Percent_Identity=44.758064516129, Blast_Score=218, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6322922, Length=248, Percent_Identity=30.6451612903226, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 54288; Mature: 54288

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQGIVYLLGAGPGDPGLITVRGRELVGMAEVLVYDALSSAEMLNWAPAACERIFVGKRA
CCCCEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCH
SRHALPQEEINALLVRLGRAGKKVVRLKGGDPYVFGRGGEEADALHEAGIPFEVVPGVTS
HHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCHHHHHHHCCCCEEECCCCHH
AIAGPAYAGIPVTHRGYCTQFTVFTGHEGENKKASSLDLRGIAEAQGTKVMLMGMRKLAD
HHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHCCCCEEEEHHHHHHHH
VCEALIGYGQEPSVPAAAVQWATTGIQRTVTGTVKTLPARVQKAGLGAPAVVVIGDVVKE
HHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHH
RESLNWFEKLPLFGKRIVVTRTRAQAGELSARLRRLGAEVLEMPVIRIAPPSNRREFAES
HHHCCHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHH
VVHAHTYDWLVFSSPNGVERFFQAFFAVYRDIRSIGGARIAAIGPGTEAKLREYGLAVDV
HHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCEEEC
MPKKFVAEGLVKAFRDAREEIGTIEHSTFLWVRGEEARRVIYDGLNALGAIVDECIAYKT
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
EAETEDVAGAQAAFRERGADIVTFTSSSTAENFFKLGLPWPEGCRAASIGPVTTATLKEL
CCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHEECCCCCCCCCCCCCCCCHHHHHHHC
GHAPSITAKTHDINGLVEAIVKAAGK
CCCCCCEECCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQQGIVYLLGAGPGDPGLITVRGRELVGMAEVLVYDALSSAEMLNWAPAACERIFVGKRA
CCCCEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCH
SRHALPQEEINALLVRLGRAGKKVVRLKGGDPYVFGRGGEEADALHEAGIPFEVVPGVTS
HHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCHHHHHHHCCCCEEECCCCHH
AIAGPAYAGIPVTHRGYCTQFTVFTGHEGENKKASSLDLRGIAEAQGTKVMLMGMRKLAD
HHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHCCCCEEEEHHHHHHHH
VCEALIGYGQEPSVPAAAVQWATTGIQRTVTGTVKTLPARVQKAGLGAPAVVVIGDVVKE
HHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHH
RESLNWFEKLPLFGKRIVVTRTRAQAGELSARLRRLGAEVLEMPVIRIAPPSNRREFAES
HHHCCHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHH
VVHAHTYDWLVFSSPNGVERFFQAFFAVYRDIRSIGGARIAAIGPGTEAKLREYGLAVDV
HHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCEEEC
MPKKFVAEGLVKAFRDAREEIGTIEHSTFLWVRGEEARRVIYDGLNALGAIVDECIAYKT
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
EAETEDVAGAQAAFRERGADIVTFTSSSTAENFFKLGLPWPEGCRAASIGPVTTATLKEL
CCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHEECCCCCCCCCCCCCCCCHHHHHHHC
GHAPSITAKTHDINGLVEAIVKAAGK
CCCCCCEECCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]