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The map label for this gene is topB [H]

Identifier: 187735400

GI number: 187735400

Start: 1070694

End: 1073303

Strand: Direct

Name: topB [H]

Synonym: Amuc_0898

Alternate gene names: 187735400

Gene position: 1070694-1073303 (Clockwise)

Preceding gene: 187735398

Following gene: 187735401

Centisome position: 40.19

GC content: 58.66

Gene sequence:

>2610_bases
ATGTCCAAAACGTTAATTATAGCAGAAAAGCCGAGCGTCGCCACTGATTTGGCGAGAGTGCTCGGCAAAGAGCTTGGAAA
ATTCACCCGGGACAAGTCCGGGGCATTTTACCAGAATGACCGGGCCATCATCACCTCTGCCGTAGGCCATCTGCTGGAAC
AGAAAAAACCCATGACGGAAGAAGGAAAGTCCCTGCCGTGGAAATTCGACTATCTGCCCGTCATCCCCAGGAAGTTTGAA
CTGGAACCCATCAAGCAGTCGGAAGACCGTTTGAAAAAGGTTCTTCAGCTTGCCAGGAGCAAGGACGTCACGGAAATCGT
CAACGCATGCGATGCCGGGCGTGAAGGGGAACTGATCTTTCACAATCTGGCGCGTTACGGAAAATGGACCAAGCCTACGC
GGCGCTTATGGATGCAGTCCATGACAGATGAGGCCATCCTCAACGCATGGCATGGCATGCGCAGCGAAGCTGACATGAAG
CCCCTGACGAATGCCGCCGTGTGCCGTTCCGAATCAGACTGGCTGGTGGGGCTGAACAGCACCCGCGCCTTGACGATCCT
GCAATCCCGCGGCGGCTTCAACGTCACCCCCGCCGGGCGCGTGCAGACGCCCACGCTGGCTATTCTGACGCAGAGGGAAT
TGGAAATCCAGGCGTTTGAACCGGAGCCCTATTCGGAAGTATGGGCCGAATTCGACGTTCAGTCCGGCTCTTACTCCGGC
CGCTGGATTGACCGTGACTGGAAGAAAAACGATCATCCCCACTCCCGCGCGGAGCGCATCTGGGATCCGGAACAGGCGGC
CGTCATCCGCGACCGCTGCCGCGGCAAAGCCGGCACGGTGACGGAAGAAAAGAAACCGGTCACGACGGTCGCCCCGCTGT
TGTATGACCTGACTTCCCTTCAGCGGGAAGCCGCCAACCGCTACGGTTTTTCCGCCAAGCGCACGCTGCAGCTGGCCCAG
GAATGCTATGAAAAGCACAAGGTGCTCACTTATCCGCGTACGGACTCCCGCTACCTGCCGGAAGATTATCTGGGCAAGGT
TTACGGCATCGTAGGCACGGTGGCGGAGCAGAACCCGGAATTCGCCCCCTTTGCCAGGGACATTCTGGACAACAAGCGCA
TCAAGCCCAACCGGCGCGTGTTTGATACAAAAAAGGTGAGCGACCACTTTGCCATCATCCCGACCGGGAAGATGGAGAAG
CTTACCGGGGACGCGCAGAAACTCTTTGAAATGGTCATGGCCCGTTTCCTGGCCGTCTTCTTCCCCGCTGCCGTGTTTGA
AGACACGCGGAGAACCACGCTCATTACCCACCAGGACGGCGTGACGGACGCTTTCCTGACCACGGGCCGGGTGCTTGTGG
AGCCGGGCTGGCAGGCCGTTTACGGACGGAAGGCCGGCACTTCCTCCAAGGAGGAGCTGGTGCCCGTCCGCGACGGGGAG
CAGGCCGCCTTGCGTGAGATTGAAATCCGCAACGCCATGACCAAGCCGCCCGCCCGGTACAACGAAGCCACGCTGCTGGC
TGCCATGGAGGGGGCAGGAAAACTGGTGCATGACGAAGAACTGGCCGCCGCCATGAGCGAACGCGGCCTGGGCACTCCCG
CCACCCGCGCCTCCATCATTGAAGGGCTGATTTCCCAGGAATACATCGTCCGGGACGGCCGCGAACTTCTGGCGACGCGC
CGCGGGATTGAACTGATTGCCCTGCTGGAACGCATCGGCCTGAAAACGCTCACCTCCCCCAGCCTGACTGGGGAATGGGA
ATACAAGCTCAAGCAGATGGAACAGGCCGCCCTGCCGAGGGATTCCTTCATGGAGGGCATTAAGACCCTGACCGGGGAAA
TCGTGAAGAGGGTGAAGGATTTCCGGGAAGCCCAGCAGCAGGTGGAACTGCCGGAAATGGAATTGGACTGCCCTTCCTGC
CATGCCCACGGCCTGAAAAACACGCTGGACGCCGTTTCCTGCCGTTCCTGCAAGTTCAGCATCCGCAAAGTTATCTCCGG
CCGCGACATGGCGGATGAAGAATTGAAGACCCTTATTGTGGACGGCAGGACGGGAATTCTCCACGGATTTACCTCCCGGT
TCGGCAAGCCCTTTGAGGCGGGGCTGGAACTCAACGACAAATTCCGGGCCACGCTTTACTTCCCGGCGCGGGAGGAAGAT
GAAGCCGCAGGGGTGGAGGAAGCCGTGAAAGTGGCTTCCGTCGCCATTCCGGACATGGGAGAGCATGATGTGATGGAAAC
GGCCAAGGCCTGGAAAATTCCTTCCCTGACCCTTGGAAAGGAACATGCCGCCGTCTCCGTTTCCCGGACTATCCTGGGAC
GTGAAATCCCGCTGGACCAGGTATTGAAGATTTTGGCGGAAGGAAAATCCGATCTGATGAAGGGGTTCATTTCGCAGCGT
ACCAAGCGTCCGTTTGACGCTTATCTGGTCTTCAATGCAAAGACGGGGAAAATAGGTTTTGAATTCCCGCCGCGGGAAAG
GAAATATCCCGCTAAAAACGCTGCCGGAAAAACGGAAGGCAAGGCGGAGAAGACGGGCCGGAACGCCGCCAAAACTGCCG
GGAAAGACTCCAAATCCGGGGGGGCTGCCAAAAAGGGGCGCAAGCAATAA

Upstream 100 bases:

>100_bases
GGGGGTAGGGGAGTAGGAATTCAGCTCCAAATGCAACGCCCCCTTCCCCGTGCGGGCAGCGTCGCTTTTATTGTTCAGCA
CTTTTTTCCGAGCCGCCGAT

Downstream 100 bases:

>100_bases
CGGCAGCGGAAACATTCCGCCGGGAACGAAGATTGGGAAAAGAGCCGGGAAAAAGCTTCTTCCCGGCTGTTCCCGTATGT
TAGCTTGAAAGCGCACCCCT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 869; Mature: 868

Protein sequence:

>869_residues
MSKTLIIAEKPSVATDLARVLGKELGKFTRDKSGAFYQNDRAIITSAVGHLLEQKKPMTEEGKSLPWKFDYLPVIPRKFE
LEPIKQSEDRLKKVLQLARSKDVTEIVNACDAGREGELIFHNLARYGKWTKPTRRLWMQSMTDEAILNAWHGMRSEADMK
PLTNAAVCRSESDWLVGLNSTRALTILQSRGGFNVTPAGRVQTPTLAILTQRELEIQAFEPEPYSEVWAEFDVQSGSYSG
RWIDRDWKKNDHPHSRAERIWDPEQAAVIRDRCRGKAGTVTEEKKPVTTVAPLLYDLTSLQREAANRYGFSAKRTLQLAQ
ECYEKHKVLTYPRTDSRYLPEDYLGKVYGIVGTVAEQNPEFAPFARDILDNKRIKPNRRVFDTKKVSDHFAIIPTGKMEK
LTGDAQKLFEMVMARFLAVFFPAAVFEDTRRTTLITHQDGVTDAFLTTGRVLVEPGWQAVYGRKAGTSSKEELVPVRDGE
QAALREIEIRNAMTKPPARYNEATLLAAMEGAGKLVHDEELAAAMSERGLGTPATRASIIEGLISQEYIVRDGRELLATR
RGIELIALLERIGLKTLTSPSLTGEWEYKLKQMEQAALPRDSFMEGIKTLTGEIVKRVKDFREAQQQVELPEMELDCPSC
HAHGLKNTLDAVSCRSCKFSIRKVISGRDMADEELKTLIVDGRTGILHGFTSRFGKPFEAGLELNDKFRATLYFPAREED
EAAGVEEAVKVASVAIPDMGEHDVMETAKAWKIPSLTLGKEHAAVSVSRTILGREIPLDQVLKILAEGKSDLMKGFISQR
TKRPFDAYLVFNAKTGKIGFEFPPRERKYPAKNAAGKTEGKAEKTGRNAAKTAGKDSKSGGAAKKGRKQ

Sequences:

>Translated_869_residues
MSKTLIIAEKPSVATDLARVLGKELGKFTRDKSGAFYQNDRAIITSAVGHLLEQKKPMTEEGKSLPWKFDYLPVIPRKFE
LEPIKQSEDRLKKVLQLARSKDVTEIVNACDAGREGELIFHNLARYGKWTKPTRRLWMQSMTDEAILNAWHGMRSEADMK
PLTNAAVCRSESDWLVGLNSTRALTILQSRGGFNVTPAGRVQTPTLAILTQRELEIQAFEPEPYSEVWAEFDVQSGSYSG
RWIDRDWKKNDHPHSRAERIWDPEQAAVIRDRCRGKAGTVTEEKKPVTTVAPLLYDLTSLQREAANRYGFSAKRTLQLAQ
ECYEKHKVLTYPRTDSRYLPEDYLGKVYGIVGTVAEQNPEFAPFARDILDNKRIKPNRRVFDTKKVSDHFAIIPTGKMEK
LTGDAQKLFEMVMARFLAVFFPAAVFEDTRRTTLITHQDGVTDAFLTTGRVLVEPGWQAVYGRKAGTSSKEELVPVRDGE
QAALREIEIRNAMTKPPARYNEATLLAAMEGAGKLVHDEELAAAMSERGLGTPATRASIIEGLISQEYIVRDGRELLATR
RGIELIALLERIGLKTLTSPSLTGEWEYKLKQMEQAALPRDSFMEGIKTLTGEIVKRVKDFREAQQQVELPEMELDCPSC
HAHGLKNTLDAVSCRSCKFSIRKVISGRDMADEELKTLIVDGRTGILHGFTSRFGKPFEAGLELNDKFRATLYFPAREED
EAAGVEEAVKVASVAIPDMGEHDVMETAKAWKIPSLTLGKEHAAVSVSRTILGREIPLDQVLKILAEGKSDLMKGFISQR
TKRPFDAYLVFNAKTGKIGFEFPPRERKYPAKNAAGKTEGKAEKTGRNAAKTAGKDSKSGGAAKKGRKQ
>Mature_868_residues
SKTLIIAEKPSVATDLARVLGKELGKFTRDKSGAFYQNDRAIITSAVGHLLEQKKPMTEEGKSLPWKFDYLPVIPRKFEL
EPIKQSEDRLKKVLQLARSKDVTEIVNACDAGREGELIFHNLARYGKWTKPTRRLWMQSMTDEAILNAWHGMRSEADMKP
LTNAAVCRSESDWLVGLNSTRALTILQSRGGFNVTPAGRVQTPTLAILTQRELEIQAFEPEPYSEVWAEFDVQSGSYSGR
WIDRDWKKNDHPHSRAERIWDPEQAAVIRDRCRGKAGTVTEEKKPVTTVAPLLYDLTSLQREAANRYGFSAKRTLQLAQE
CYEKHKVLTYPRTDSRYLPEDYLGKVYGIVGTVAEQNPEFAPFARDILDNKRIKPNRRVFDTKKVSDHFAIIPTGKMEKL
TGDAQKLFEMVMARFLAVFFPAAVFEDTRRTTLITHQDGVTDAFLTTGRVLVEPGWQAVYGRKAGTSSKEELVPVRDGEQ
AALREIEIRNAMTKPPARYNEATLLAAMEGAGKLVHDEELAAAMSERGLGTPATRASIIEGLISQEYIVRDGRELLATRR
GIELIALLERIGLKTLTSPSLTGEWEYKLKQMEQAALPRDSFMEGIKTLTGEIVKRVKDFREAQQQVELPEMELDCPSCH
AHGLKNTLDAVSCRSCKFSIRKVISGRDMADEELKTLIVDGRTGILHGFTSRFGKPFEAGLELNDKFRATLYFPAREEDE
AAGVEEAVKVASVAIPDMGEHDVMETAKAWKIPSLTLGKEHAAVSVSRTILGREIPLDQVLKILAEGKSDLMKGFISQRT
KRPFDAYLVFNAKTGKIGFEFPPRERKYPAKNAAGKTEGKAEKTGRNAAKTAGKDSKSGGAAKKGRKQ

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=683, Percent_Identity=23.8653001464129, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI4507635, Length=635, Percent_Identity=25.0393700787402, Blast_Score=108, Evalue=3e-23,
Organism=Escherichia coli, GI1788061, Length=633, Percent_Identity=30.8056872037915, Blast_Score=285, Evalue=1e-77,
Organism=Escherichia coli, GI1787529, Length=677, Percent_Identity=25.8493353028065, Blast_Score=138, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17555378, Length=636, Percent_Identity=26.1006289308176, Blast_Score=114, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24585251, Length=659, Percent_Identity=23.2169954476479, Blast_Score=94, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 97194; Mature: 97063

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTLIIAEKPSVATDLARVLGKELGKFTRDKSGAFYQNDRAIITSAVGHLLEQKKPMTE
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCCCCCC
EGKSLPWKFDYLPVIPRKFELEPIKQSEDRLKKVLQLARSKDVTEIVNACDAGREGELIF
CCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHH
HNLARYGKWTKPTRRLWMQSMTDEAILNAWHGMRSEADMKPLTNAAVCRSESDWLVGLNS
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCEEEECCC
TRALTILQSRGGFNVTPAGRVQTPTLAILTQRELEIQAFEPEPYSEVWAEFDVQSGSYSG
CEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHCCCCCCCCC
RWIDRDWKKNDHPHSRAERIWDPEQAAVIRDRCRGKAGTVTEEKKPVTTVAPLLYDLTSL
CCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
QREAANRYGFSAKRTLQLAQECYEKHKVLTYPRTDSRYLPEDYLGKVYGIVGTVAEQNPE
HHHHHHHCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
FAPFARDILDNKRIKPNRRVFDTKKVSDHFAIIPTGKMEKLTGDAQKLFEMVMARFLAVF
CCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHH
FPAAVFEDTRRTTLITHQDGVTDAFLTTGRVLVEPGWQAVYGRKAGTSSKEELVPVRDGE
HHHHHHHCCCCEEEEEECCCCCHHHHHCCEEEECCCCHHHHCCCCCCCCHHCCCCCCCCC
QAALREIEIRNAMTKPPARYNEATLLAAMEGAGKLVHDEELAAAMSERGLGTPATRASII
HHHHHHHHHHHHCCCCCCCCCCHHEEEEHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHH
EGLISQEYIVRDGRELLATRRGIELIALLERIGLKTLTSPSLTGEWEYKLKQMEQAALPR
HHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHCCH
DSFMEGIKTLTGEIVKRVKDFREAQQQVELPEMELDCPSCHAHGLKNTLDAVSCRSCKFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IRKVISGRDMADEELKTLIVDGRTGILHGFTSRFGKPFEAGLELNDKFRATLYFPAREED
HHHHHCCCCCCHHHHHEEEECCCCHHHHHHHHHCCCCHHCCCCCCCCEEEEEEECCCCCH
EAAGVEEAVKVASVAIPDMGEHDVMETAKAWKIPSLTLGKEHAAVSVSRTILGREIPLDQ
HHCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHH
VLKILAEGKSDLMKGFISQRTKRPFDAYLVFNAKTGKIGFEFPPRERKYPAKNAAGKTEG
HHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
KAEKTGRNAAKTAGKDSKSGGAAKKGRKQ
CHHHCCCHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKTLIIAEKPSVATDLARVLGKELGKFTRDKSGAFYQNDRAIITSAVGHLLEQKKPMTE
CCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCCCCCC
EGKSLPWKFDYLPVIPRKFELEPIKQSEDRLKKVLQLARSKDVTEIVNACDAGREGELIF
CCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHH
HNLARYGKWTKPTRRLWMQSMTDEAILNAWHGMRSEADMKPLTNAAVCRSESDWLVGLNS
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCEEEECCC
TRALTILQSRGGFNVTPAGRVQTPTLAILTQRELEIQAFEPEPYSEVWAEFDVQSGSYSG
CEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHCCCCCCCCC
RWIDRDWKKNDHPHSRAERIWDPEQAAVIRDRCRGKAGTVTEEKKPVTTVAPLLYDLTSL
CCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
QREAANRYGFSAKRTLQLAQECYEKHKVLTYPRTDSRYLPEDYLGKVYGIVGTVAEQNPE
HHHHHHHCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
FAPFARDILDNKRIKPNRRVFDTKKVSDHFAIIPTGKMEKLTGDAQKLFEMVMARFLAVF
CCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHH
FPAAVFEDTRRTTLITHQDGVTDAFLTTGRVLVEPGWQAVYGRKAGTSSKEELVPVRDGE
HHHHHHHCCCCEEEEEECCCCCHHHHHCCEEEECCCCHHHHCCCCCCCCHHCCCCCCCCC
QAALREIEIRNAMTKPPARYNEATLLAAMEGAGKLVHDEELAAAMSERGLGTPATRASII
HHHHHHHHHHHHCCCCCCCCCCHHEEEEHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHH
EGLISQEYIVRDGRELLATRRGIELIALLERIGLKTLTSPSLTGEWEYKLKQMEQAALPR
HHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHCCH
DSFMEGIKTLTGEIVKRVKDFREAQQQVELPEMELDCPSCHAHGLKNTLDAVSCRSCKFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IRKVISGRDMADEELKTLIVDGRTGILHGFTSRFGKPFEAGLELNDKFRATLYFPAREED
HHHHHCCCCCCHHHHHEEEECCCCHHHHHHHHHCCCCHHCCCCCCCCEEEEEEECCCCCH
EAAGVEEAVKVASVAIPDMGEHDVMETAKAWKIPSLTLGKEHAAVSVSRTILGREIPLDQ
HHCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHH
VLKILAEGKSDLMKGFISQRTKRPFDAYLVFNAKTGKIGFEFPPRERKYPAKNAAGKTEG
HHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
KAEKTGRNAAKTAGKDSKSGGAAKKGRKQ
CHHHCCCHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]