Click here to switch to the map view.

The map label for this gene is nfo

Identifier: 187735347

GI number: 187735347

Start: 1006117

End: 1006950

Strand: Reverse

Name: nfo

Synonym: Amuc_0845

Alternate gene names: 187735347

Gene position: 1006950-1006117 (Counterclockwise)

Preceding gene: 187735351

Following gene: 187735346

Centisome position: 37.8

GC content: 54.68

Gene sequence:

>834_bases
ATGCCATACATCGGATGCCACCTCTCCTCCGCAAAAGGCTATGAAGCCATGGGCCGGGTTGCCCTCTCCATCGGCGCCAA
TACTTTTCAGTTCTTTACCCGCAATCCGCGGGGGAGCAAAGCCAAAGCTATTGACGAACAGGATATTGCCCGTTTTCTGG
AATTAGCGCGAAATAACGGATTCGGCACGCTGCTGGCACACGCCCCCTACACGTTGAACCCCTGTTCCGCAGACCCCAGT
GTCGCCCGTTTTGCGGCCCAGGTACTGAAAGAGGATCTGGAACTCATGGAACATCTGCCGGGGAATCTTTATAACTTCCA
TCCCGGCTGCCACGTAGGGCAGGGAGTGGAAAAAGGCATTGAACTGGTGGCGGATCAATTAAACGACGTGCTTTCGCCGG
AACAAAAGACCATTGTTTTGCTGGAAACCATGTCCGGCAAGGGAAGCGAAGTCGGCCGGACCTTTGAAGAACTGGCCGCC
ATCATGGAGCGGGTGGACCTGAAGGACAAACTTGGCGTATGCCTGGATACCTGCCATGTCTATTCCGCCGGATATGATAT
CGTCAACAGGCTGGATTCCGTACTGGAACATTTTGATGCCGTGCTGGGTCTGGAACGCCTGCGCGCCATCCATCTGAATG
ACAGCATGACACCCTTTTCCTCCTTCAAGGACCGCCATGAAACCATCGGCAAGGGTTCCCTGGGAGAACAGGCCTTCATC
AACATCATCAACCATCCCGTTCTGCGGGAATTGCCCTTTTTCCTGGAAACGCCCAGGGACGACGCCGGGCACGGCGAAGA
AATAACGTGGCTGAAGGAACATTACCGCAACTGA

Upstream 100 bases:

>100_bases
CTGGCGGTCTTATTCTTTCCGATCAAGTCATGCAGGCCTTTTAGTCTCTCTTCCTCTCTGCGGCATTGATAAACTTCCTT
TCCATTCCGAACTTACCGCC

Downstream 100 bases:

>100_bases
ACCGCCGCCAAGGGGTTTCCGTACTGATTGAACTCCGGTCTTTCTCCCGGAGAAAAAGCTTGCATCCGCTCCGGATACGG
GATACACAAACCTTGTGAAT

Product: apurinic endonuclease Apn1

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MPYIGCHLSSAKGYEAMGRVALSIGANTFQFFTRNPRGSKAKAIDEQDIARFLELARNNGFGTLLAHAPYTLNPCSADPS
VARFAAQVLKEDLELMEHLPGNLYNFHPGCHVGQGVEKGIELVADQLNDVLSPEQKTIVLLETMSGKGSEVGRTFEELAA
IMERVDLKDKLGVCLDTCHVYSAGYDIVNRLDSVLEHFDAVLGLERLRAIHLNDSMTPFSSFKDRHETIGKGSLGEQAFI
NIINHPVLRELPFFLETPRDDAGHGEEITWLKEHYRN

Sequences:

>Translated_277_residues
MPYIGCHLSSAKGYEAMGRVALSIGANTFQFFTRNPRGSKAKAIDEQDIARFLELARNNGFGTLLAHAPYTLNPCSADPS
VARFAAQVLKEDLELMEHLPGNLYNFHPGCHVGQGVEKGIELVADQLNDVLSPEQKTIVLLETMSGKGSEVGRTFEELAA
IMERVDLKDKLGVCLDTCHVYSAGYDIVNRLDSVLEHFDAVLGLERLRAIHLNDSMTPFSSFKDRHETIGKGSLGEQAFI
NIINHPVLRELPFFLETPRDDAGHGEEITWLKEHYRN
>Mature_276_residues
PYIGCHLSSAKGYEAMGRVALSIGANTFQFFTRNPRGSKAKAIDEQDIARFLELARNNGFGTLLAHAPYTLNPCSADPSV
ARFAAQVLKEDLELMEHLPGNLYNFHPGCHVGQGVEKGIELVADQLNDVLSPEQKTIVLLETMSGKGSEVGRTFEELAAI
MERVDLKDKLGVCLDTCHVYSAGYDIVNRLDSVLEHFDAVLGLERLRAIHLNDSMTPFSSFKDRHETIGKGSLGEQAFIN
IINHPVLRELPFFLETPRDDAGHGEEITWLKEHYRN

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=277, Percent_Identity=33.5740072202166, Blast_Score=158, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17531193, Length=260, Percent_Identity=34.6153846153846, Blast_Score=167, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6322735, Length=277, Percent_Identity=31.7689530685921, Blast_Score=153, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_AKKM8 (B2UQE3)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877459.1
- ProteinModelPortal:   B2UQE3
- SMR:   B2UQE3
- GeneID:   6274324
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0845
- HOGENOM:   HBG565018
- OMA:   QIALETM
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 30780; Mature: 30649

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYIGCHLSSAKGYEAMGRVALSIGANTFQFFTRNPRGSKAKAIDEQDIARFLELARNNG
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC
FGTLLAHAPYTLNPCSADPSVARFAAQVLKEDLELMEHLPGNLYNFHPGCHVGQGVEKGI
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
ELVADQLNDVLSPEQKTIVLLETMSGKGSEVGRTFEELAAIMERVDLKDKLGVCLDTCHV
HHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
YSAGYDIVNRLDSVLEHFDAVLGLERLRAIHLNDSMTPFSSFKDRHETIGKGSLGEQAFI
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
NIINHPVLRELPFFLETPRDDAGHGEEITWLKEHYRN
HHHCCHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
PYIGCHLSSAKGYEAMGRVALSIGANTFQFFTRNPRGSKAKAIDEQDIARFLELARNNG
CCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCC
FGTLLAHAPYTLNPCSADPSVARFAAQVLKEDLELMEHLPGNLYNFHPGCHVGQGVEKGI
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
ELVADQLNDVLSPEQKTIVLLETMSGKGSEVGRTFEELAAIMERVDLKDKLGVCLDTCHV
HHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
YSAGYDIVNRLDSVLEHFDAVLGLERLRAIHLNDSMTPFSSFKDRHETIGKGSLGEQAFI
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
NIINHPVLRELPFFLETPRDDAGHGEEITWLKEHYRN
HHHCCHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA