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The map label for this gene is eno [H]

Identifier: 187735346

GI number: 187735346

Start: 1004673

End: 1005962

Strand: Reverse

Name: eno [H]

Synonym: Amuc_0844

Alternate gene names: 187735346

Gene position: 1005962-1004673 (Counterclockwise)

Preceding gene: 187735347

Following gene: 187735345

Centisome position: 37.76

GC content: 58.14

Gene sequence:

>1290_bases
ATGAAAGAAATGCGCATCGCGAAAATAACAGGGCGTGAAATTCTGGATTCACGCGGCAACCCCACCATTGAGGCGGACGT
TTATCTGGAAGGAGGCGCCGTAGGCACGGCGGGCGTCCCCAGCGGAGCTTCCACCGGGGAACATGAAGCCTGGGAACTCA
GGGATGGCGACACAACCCGCTACCAGGGTAAGGGAGTCACCAAGGCCGTAGACAACATCCGTAACGTCATTGCTCCGGCC
CTGATCGGTCTGGAAGCCACGGAACAAACCACGGCGGATGCCCTCATGAATTCCCTGGACGGCACATTCAACAAGGCCAA
ACTGGGGGCCAACGCCATTCTGGGTGTTTCCCTGGCGCTTGCCAAGGCGGCGGCGGCCCAGCTGCATCAGCCCCTGTACC
GCTACCTGGGCGGCCCGAACGCCAAGGTCCTTCCCGTTCCGATGATGAACATCATCAACGGAGGCGCCCACTCCGACGCG
CCTATTGATTTCCAGGAATTCATGATCGTCCCGTCCGGCGCTCCCACCTTCCGGGAAGCTCTACGTTATGGCGCGGAAGT
TTTCCATGCGCTGAAATCCGTTCTTCATGACCGCGGCCTCTCCACCGCCGTGGGCGACGAAGGCGGCTTCGCCCCCAAGC
TGAACTCTGCGGAAGACGCCCTGGATTGCATCGCCCAGGCGGTGGACAAGGCCGGTTACCGGTTCGGGGAGGACATTTCC
ATTGCCCTGGACGTGGCTTCCTCCGAATTTTATGAACCGGAATCCGGGCTGTATGTTTTCAAGAAATCCGACGGCAGCCG
CAGAACCGCCGCAGAGCTGACGGACTACTATGTGGAACTGAAGAAAAAATATCCCATCGTCTCCATCGAAGACGGCTGTG
CGGAAAACGACTGGGACGGATGGAAAATCCTGACGGAAAAACTTGGCAAAAGCACTCAGCTTGTCGGAGATGACCTCTTT
GTCACCAACGTGGAATTCCTCAGAAAAGGCATCCATCTGGGCGTGGGGAACTCCATCCTGGTGAAGGTCAATCAAATCGG
CTCGCTGACGGAAACGCTGGACGCCGTGGAGCTGGCCATGGGCAACCGCTATACCGCCGTTATTTCCCACCGTTCCGGAG
AAACGGAGGACGCCACCATCGCGGACATTGCCGTAGGAACCAACGCCGGACAAATCAAAACCGGCTCTTTGAGCCGTTCC
GACCGTATGGCCAAATATAACCGCCTTCTCCGGATTGAAGAAGAACTGGGAGAATCCGCCCGCTACGGCAACGGATTCGG
CCTGTTCTGA

Upstream 100 bases:

>100_bases
AAAGCTTGCATCCGCTCCGGATACGGGATACACAAACCTTGTGAATTAGTTTAAACTAATTCCAATATCTGGTTTCCAAG
GAAAAAACCATCCGATTTTT

Downstream 100 bases:

>100_bases
CCTTTTTGCTGGAACTGGTATCAAGCAACGCCAAACCCCGGCCGCCGCGAGGCGGTGCGGGGATTTTTCATCTCCGGAGG
CAACTGCATCCTTTTCTTTG

Product: Phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKEMRIAKITGREILDSRGNPTIEADVYLEGGAVGTAGVPSGASTGEHEAWELRDGDTTRYQGKGVTKAVDNIRNVIAPA
LIGLEATEQTTADALMNSLDGTFNKAKLGANAILGVSLALAKAAAAQLHQPLYRYLGGPNAKVLPVPMMNIINGGAHSDA
PIDFQEFMIVPSGAPTFREALRYGAEVFHALKSVLHDRGLSTAVGDEGGFAPKLNSAEDALDCIAQAVDKAGYRFGEDIS
IALDVASSEFYEPESGLYVFKKSDGSRRTAAELTDYYVELKKKYPIVSIEDGCAENDWDGWKILTEKLGKSTQLVGDDLF
VTNVEFLRKGIHLGVGNSILVKVNQIGSLTETLDAVELAMGNRYTAVISHRSGETEDATIADIAVGTNAGQIKTGSLSRS
DRMAKYNRLLRIEEELGESARYGNGFGLF

Sequences:

>Translated_429_residues
MKEMRIAKITGREILDSRGNPTIEADVYLEGGAVGTAGVPSGASTGEHEAWELRDGDTTRYQGKGVTKAVDNIRNVIAPA
LIGLEATEQTTADALMNSLDGTFNKAKLGANAILGVSLALAKAAAAQLHQPLYRYLGGPNAKVLPVPMMNIINGGAHSDA
PIDFQEFMIVPSGAPTFREALRYGAEVFHALKSVLHDRGLSTAVGDEGGFAPKLNSAEDALDCIAQAVDKAGYRFGEDIS
IALDVASSEFYEPESGLYVFKKSDGSRRTAAELTDYYVELKKKYPIVSIEDGCAENDWDGWKILTEKLGKSTQLVGDDLF
VTNVEFLRKGIHLGVGNSILVKVNQIGSLTETLDAVELAMGNRYTAVISHRSGETEDATIADIAVGTNAGQIKTGSLSRS
DRMAKYNRLLRIEEELGESARYGNGFGLF
>Mature_429_residues
MKEMRIAKITGREILDSRGNPTIEADVYLEGGAVGTAGVPSGASTGEHEAWELRDGDTTRYQGKGVTKAVDNIRNVIAPA
LIGLEATEQTTADALMNSLDGTFNKAKLGANAILGVSLALAKAAAAQLHQPLYRYLGGPNAKVLPVPMMNIINGGAHSDA
PIDFQEFMIVPSGAPTFREALRYGAEVFHALKSVLHDRGLSTAVGDEGGFAPKLNSAEDALDCIAQAVDKAGYRFGEDIS
IALDVASSEFYEPESGLYVFKKSDGSRRTAAELTDYYVELKKKYPIVSIEDGCAENDWDGWKILTEKLGKSTQLVGDDLF
VTNVEFLRKGIHLGVGNSILVKVNQIGSLTETLDAVELAMGNRYTAVISHRSGETEDATIADIAVGTNAGQIKTGSLSRS
DRMAKYNRLLRIEEELGESARYGNGFGLF

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=433, Percent_Identity=53.1177829099307, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=51.6279069767442, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI301897477, Length=429, Percent_Identity=49.6503496503497, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI301897469, Length=429, Percent_Identity=49.6503496503497, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI301897479, Length=427, Percent_Identity=45.1990632318501, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI169201331, Length=344, Percent_Identity=26.453488372093, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI169201757, Length=344, Percent_Identity=26.453488372093, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI239744207, Length=344, Percent_Identity=26.453488372093, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1789141, Length=422, Percent_Identity=61.6113744075829, Blast_Score=491, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI71995829, Length=436, Percent_Identity=52.7522935779816, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17536383, Length=434, Percent_Identity=52.9953917050691, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI32563855, Length=300, Percent_Identity=41.6666666666667, Blast_Score=171, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6324974, Length=431, Percent_Identity=48.7238979118329, Blast_Score=376, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324969, Length=431, Percent_Identity=48.7238979118329, Blast_Score=376, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6323985, Length=431, Percent_Identity=48.7238979118329, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321693, Length=431, Percent_Identity=48.9559164733179, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6321968, Length=431, Percent_Identity=48.9559164733179, Blast_Score=347, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=49.5348837209302, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=49.5348837209302, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=49.5348837209302, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=49.5348837209302, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI281360527, Length=429, Percent_Identity=49.4172494172494, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI17137654, Length=429, Percent_Identity=49.4172494172494, Blast_Score=365, Evalue=1e-101,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45971; Mature: 45971

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEMRIAKITGREILDSRGNPTIEADVYLEGGAVGTAGVPSGASTGEHEAWELRDGDTTR
CCCCEEEECCCHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCE
YQGKGVTKAVDNIRNVIAPALIGLEATEQTTADALMNSLDGTFNKAKLGANAILGVSLAL
ECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHH
AKAAAAQLHQPLYRYLGGPNAKVLPVPMMNIINGGAHSDAPIDFQEFMIVPSGAPTFREA
HHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHCCCCCCCCCCCHHHEEEECCCCHHHHHH
LRYGAEVFHALKSVLHDRGLSTAVGDEGGFAPKLNSAEDALDCIAQAVDKAGYRFGEDIS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEE
IALDVASSEFYEPESGLYVFKKSDGSRRTAAELTDYYVELKKKYPIVSIEDGCAENDWDG
EEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCH
WKILTEKLGKSTQLVGDDLFVTNVEFLRKGIHLGVGNSILVKVNQIGSLTETLDAVELAM
HHHHHHHCCCCCEECCCCEEEECHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHH
GNRYTAVISHRSGETEDATIADIAVGTNAGQIKTGSLSRSDRMAKYNRLLRIEEELGESA
CCCEEEEEECCCCCCCCCEEEEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCHH
RYGNGFGLF
CCCCCCCCC
>Mature Secondary Structure
MKEMRIAKITGREILDSRGNPTIEADVYLEGGAVGTAGVPSGASTGEHEAWELRDGDTTR
CCCCEEEECCCHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCE
YQGKGVTKAVDNIRNVIAPALIGLEATEQTTADALMNSLDGTFNKAKLGANAILGVSLAL
ECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHH
AKAAAAQLHQPLYRYLGGPNAKVLPVPMMNIINGGAHSDAPIDFQEFMIVPSGAPTFREA
HHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHCCCCCCCCCCCHHHEEEECCCCHHHHHH
LRYGAEVFHALKSVLHDRGLSTAVGDEGGFAPKLNSAEDALDCIAQAVDKAGYRFGEDIS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEE
IALDVASSEFYEPESGLYVFKKSDGSRRTAAELTDYYVELKKKYPIVSIEDGCAENDWDG
EEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCH
WKILTEKLGKSTQLVGDDLFVTNVEFLRKGIHLGVGNSILVKVNQIGSLTETLDAVELAM
HHHHHHHCCCCCEECCCCEEEECHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHH
GNRYTAVISHRSGETEDATIADIAVGTNAGQIKTGSLSRSDRMAKYNRLLRIEEELGESA
CCCEEEEEECCCCCCCCCEEEEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCHH
RYGNGFGLF
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA