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The map label for this gene is queA [H]

Identifier: 187735219

GI number: 187735219

Start: 842050

End: 843039

Strand: Reverse

Name: queA [H]

Synonym: Amuc_0713

Alternate gene names: 187735219

Gene position: 843039-842050 (Counterclockwise)

Preceding gene: 187735223

Following gene: 187735217

Centisome position: 31.64

GC content: 55.45

Gene sequence:

>990_bases
ATGCACTTGGCCGTGCGCACCATTGATTTTGATTATCCGCTGCCGGAAGAACTGATAGCGGACCGTCCCCTTCCGGACCG
CTCCGCGTCCCGGATGATGGTCATTCACCGGGATACGGGCATGATTGAGCACAGGCATTTTTGCGATTTGCCGGAATATG
TGCGGGAAGGGGATCATTTTATCCTGAATGATACCAGGGTGGTTCCGGCAAGATATTTTTCCAACGACGGTTCCATTGAA
ATAGTACGCGCGGAGGTTCTTAATCCCCTGTTGTGGAAGTGCATGGTGCGCCCCGGACGCAAAATGAAGCTTGGGCGTAC
GGTTGCGGTCGGAGAGGCGGTGGGCACCGTGGAAGGCATTGATGAGGAAGGGTACCGCCTTATCCGGTTTGACCGGGAAG
TGGATGAGGAAAAATACGGGCGTCTGGCTCTTCCCCACTATATGAACCGGGAAAGCGACCCATCCGACAGGGAGCGTTAC
CAGACCGTTTACGCCAGGCATGAAGGGGCCATTGCCGCTCCTACGGCCGGGCTGCATTTTACGCCGGAGCTGCTGGCATC
CGTCCCCCACGATTTTCTAACCCTGCACGTGGGGGTAGGAACATTCCGTCCCGTCAAGGCGGACAGGATAGAAGACCATC
AGATGCATACGGAGCATTTTTTTCTTCCTGAAGCCGCTGCCCGGGCAGTCAATAACGCCAGAAGGGTTATTGCCGTGGGC
ACCACCAGCGTGCGGGTGCTGGAACATGTGGCAACGGCGGAAGGTCTGCCGTTGAGGGAGTGTTCCGGTTCAACGAACAT
CTTCATTTATCCTCCCTACAACTACAAGGTGGTGGACGCCCTGATCACGAATTTCCATTTGCCGCAGAGCACGCTGCTGA
TGCTTGTCAGCGCCTTTGCGGGCAGGGAGCTGGTCATGAAAGCCTATCTGGAAGCCATCCGGGAACGGTACAGATTTTTC
TCCTATGGAGACTGCATGTTGATTTTGTAA

Upstream 100 bases:

>100_bases
GTTCATGTATCCTTCCATACCATATGAACCGAAGCAGGGTAAAGATATAATTGTGTTACCTGCGGTTTAAAGGTATTGAC
CTTTCGGAGGATTCGTGGCA

Downstream 100 bases:

>100_bases
GGTCCCGGCGGTTTTTTCAAGTATCCTTTGGAAAAAGCGCGGATGGTTTTAAAGACTGTCTTCCGGAGGGTGCTGCGGGC
GGAGGGACGGCGGCGGAACG

Product: S-adenosylmethionine/tRNA-ribosyltransferase- isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MHLAVRTIDFDYPLPEELIADRPLPDRSASRMMVIHRDTGMIEHRHFCDLPEYVREGDHFILNDTRVVPARYFSNDGSIE
IVRAEVLNPLLWKCMVRPGRKMKLGRTVAVGEAVGTVEGIDEEGYRLIRFDREVDEEKYGRLALPHYMNRESDPSDRERY
QTVYARHEGAIAAPTAGLHFTPELLASVPHDFLTLHVGVGTFRPVKADRIEDHQMHTEHFFLPEAAARAVNNARRVIAVG
TTSVRVLEHVATAEGLPLRECSGSTNIFIYPPYNYKVVDALITNFHLPQSTLLMLVSAFAGRELVMKAYLEAIRERYRFF
SYGDCMLIL

Sequences:

>Translated_329_residues
MHLAVRTIDFDYPLPEELIADRPLPDRSASRMMVIHRDTGMIEHRHFCDLPEYVREGDHFILNDTRVVPARYFSNDGSIE
IVRAEVLNPLLWKCMVRPGRKMKLGRTVAVGEAVGTVEGIDEEGYRLIRFDREVDEEKYGRLALPHYMNRESDPSDRERY
QTVYARHEGAIAAPTAGLHFTPELLASVPHDFLTLHVGVGTFRPVKADRIEDHQMHTEHFFLPEAAARAVNNARRVIAVG
TTSVRVLEHVATAEGLPLRECSGSTNIFIYPPYNYKVVDALITNFHLPQSTLLMLVSAFAGRELVMKAYLEAIRERYRFF
SYGDCMLIL
>Mature_329_residues
MHLAVRTIDFDYPLPEELIADRPLPDRSASRMMVIHRDTGMIEHRHFCDLPEYVREGDHFILNDTRVVPARYFSNDGSIE
IVRAEVLNPLLWKCMVRPGRKMKLGRTVAVGEAVGTVEGIDEEGYRLIRFDREVDEEKYGRLALPHYMNRESDPSDRERY
QTVYARHEGAIAAPTAGLHFTPELLASVPHDFLTLHVGVGTFRPVKADRIEDHQMHTEHFFLPEAAARAVNNARRVIAVG
TTSVRVLEHVATAEGLPLRECSGSTNIFIYPPYNYKVVDALITNFHLPQSTLLMLVSAFAGRELVMKAYLEAIRERYRFF
SYGDCMLIL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=345, Percent_Identity=44.6376811594203, Blast_Score=262, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 37567; Mature: 37567

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLAVRTIDFDYPLPEELIADRPLPDRSASRMMVIHRDTGMIEHRHFCDLPEYVREGDHF
CCEEEEEEECCCCCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCEE
ILNDTRVVPARYFSNDGSIEIVRAEVLNPLLWKCMVRPGRKMKLGRTVAVGEAVGTVEGI
EEECCEEEEHHEECCCCCEEEEHHHHCCHHHHHHHCCCCCCEECCCEEEECHHHHHHCCC
DEEGYRLIRFDREVDEEKYGRLALPHYMNRESDPSDRERYQTVYARHEGAIAAPTAGLHF
CCCCCEEEEECCCCCHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCC
TPELLASVPHDFLTLHVGVGTFRPVKADRIEDHQMHTEHFFLPEAAARAVNNARRVIAVG
CHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHCHHHEECCHHHHHHHCCCCEEEEEC
TTSVRVLEHVATAEGLPLRECSGSTNIFIYPPYNYKVVDALITNFHLPQSTLLMLVSAFA
CHHHHHHHHHHHHCCCCHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHC
GRELVMKAYLEAIRERYRFFSYGDCMLIL
CHHHHHHHHHHHHHHHHHEEECCCEEEEC
>Mature Secondary Structure
MHLAVRTIDFDYPLPEELIADRPLPDRSASRMMVIHRDTGMIEHRHFCDLPEYVREGDHF
CCEEEEEEECCCCCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCEE
ILNDTRVVPARYFSNDGSIEIVRAEVLNPLLWKCMVRPGRKMKLGRTVAVGEAVGTVEGI
EEECCEEEEHHEECCCCCEEEEHHHHCCHHHHHHHCCCCCCEECCCEEEECHHHHHHCCC
DEEGYRLIRFDREVDEEKYGRLALPHYMNRESDPSDRERYQTVYARHEGAIAAPTAGLHF
CCCCCEEEEECCCCCHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCC
TPELLASVPHDFLTLHVGVGTFRPVKADRIEDHQMHTEHFFLPEAAARAVNNARRVIAVG
CHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHCHHHEECCHHHHHHHCCCCEEEEEC
TTSVRVLEHVATAEGLPLRECSGSTNIFIYPPYNYKVVDALITNFHLPQSTLLMLVSAFA
CHHHHHHHHHHHHCCCCHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHC
GRELVMKAYLEAIRERYRFFSYGDCMLIL
CHHHHHHHHHHHHHHHHHEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA