Click here to switch to the map view.

The map label for this gene is pcrA [H]

Identifier: 187735189

GI number: 187735189

Start: 802523

End: 804532

Strand: Direct

Name: pcrA [H]

Synonym: Amuc_0683

Alternate gene names: 187735189

Gene position: 802523-804532 (Clockwise)

Preceding gene: 187735188

Following gene: 187735191

Centisome position: 30.12

GC content: 57.31

Gene sequence:

>2010_bases
ATGGCTCGCCGATATGTCATCAGGCAGGATGCCGCTCCGGTCGCCCCCATCTCCGGGATCGACTACCGTACGGCCCTCAA
TGAAGAACAGTACGCCGCCGTTTCTTCAGAACCGGGGCCGGCTCTTGTCATCGCAGGAGCCGGATCGGGAAAAACGCGCA
CGCTGACTTATCGCGTGGCCTGGCTTCTGGATCACGGTACGGACCCATGGAACATTCTGCTGCTTACCTTCACCAATAAG
GCGGCGCGGGAAATGACGGAACGGGTGCGTTCCCTGATTCCCCTGGACTTGTCCCGCCTGTGGAGCGGCACGTTCCATTC
CATTGCCAACCGCATCCTGCGCCAGCATGCGGAATACCTGGGATATACACCGGCTTTTACAATCATGGACTCCGACGACC
GCAAATCCATGATTAAAAGCGTGGTCAAATCCCTCAAACTGGATGAGAAATCCACCCGCTTCCCCAAGCCGGAAGTTCTT
TCATCCCTGTTCAGTCTGGCAGACAACGAGGGAGCCAATATCCGGGAAACGCAGGAAAACGCCTATCCCTACCTTTCCAA
CCACCTGGACGCCATCCTGGAGGTGCATGATCAATACGTAGCCCGCAAGCAGGAAACCAACAGTATGGACTTTGACGACC
TGCTTCTCAACGTCGTGAGGCTTTTTCAGGAACAGGAGCACCTGCGTGCCCTGTACGGTTCCCGCTTCCAGCATATTCTG
GTGGATGAGTACCAGGACACCAATTACCTCCAGAGCCGCCTCATCGACATGCTGGCCCGGGAACACGGCCAGCTGATGGT
GGTGGGGGACGATGCCCAGAGCATCTACTCCTGGCGCGGAGCGGACATGGAAAACATCCTCAGCTTCACTACCCGCTATC
CGGCGGCAAAGACTTTCAAGATAGAAACCAATTACCGCAGTGTGCCGGAAATTCTGGAACTTTCCAACGCGGCTATCGCC
GCCAACGAAGTTCAAATTGAAAAACAGCTCCGCTCCGTGCGGCCCACCGGGGAAATGCCTCCCGCCCTCGTCCCGCTCAA
CGACGACCGGATGCAGGCCAAATTTGTCGTCCAGCGTATCAGGGATCTCATTGAAGAAGGCACGGACCCCAGTGAAATAG
CCGTGCTTTACCGCGCCCACTTCCACAGCATGGAATTGCAAATGGAACTGACCCGCGGAGAAATACCTTTCCGCATCACC
AGCGGCATCAGGTTTTTCGAGCAGGCCCATATTAAGGACGTGGTGGCATTTATGCGTTTTGTGGTCAATCCCAGGGATGA
ACTTTCCTTCCGCCGCATGGTGATGCTGCTGCCCGGCATCGGCCCCGGCATGGCCCAAAAACTCTGGATACGCTGGACCG
CCTGCCCGGAAAGCCGGCAAGAAACCCCTCCGGAATCCTTTTCCGCCCTGATGCTGGATTTCCCGGTTCCTGCCAAATCC
AGAACGGCCTGGACGCAGCTTTGCTACACACTGGATGAACTGGCCCCTGCCGGAAAGCTGGCCGGACCGGCCTCCATGCT
CCTTTCCATCAATGAGGCCGTGTACGACGAGTACATGCAGGCCGCATTCGAAAATTACGACCAGCGCAGGCAGGACCTTC
TGCATCTGGCCGGATTTTCCGAACGCTTTGAATCCACGGAGGATTTTCTTTCCCAGCTTTCCCTTTTGGGCAACACGGAT
GATGAAAGCGCCGCGGCGGACTTTTCCGGTGGCTCCGTCACCCTGTCCACTATCCACCAGGCCAAGGGACTGGAATGGTC
TGTCGTCTTCCTGATCGGCCTGTGCGACGGCATGTTTCCGCACCAGCGGGTCATTGATGACGGAGACCTGGCCGGGCTGG
AAGAAGAACGCCGCCTCTTTTATGTAGGCATTACCCGCGCCAAGGACCAGCTTTACCTGACCTACCCCCGCTGGAACTGC
CGCGCCCAGGGAGGAACCTTCATGCAAATGCCCTCCCGCTTTCTGGATGAATTCCCAGCCGGCCTGGTGGAAGAGTGGGA
GGTGGAATAA

Upstream 100 bases:

>100_bases
ATGTACCTGGACTTTTAAAAGAATCATCAGAGAGCAGAGAAGGGGCAAGCCTTTCCAACTTCTCTATTTACTGTTGCCTG
TCAATTATTAACCATATTCC

Downstream 100 bases:

>100_bases
TCCAGATCTCCTCTCCCGGGTCATATCCGGGAAACGCCGGTTCTCAATCTTCTCCCGCCGGACAGGGAAAACGTGGCGCG
GAGTTTCCGAAGGGTTCTTT

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 669; Mature: 668

Protein sequence:

>669_residues
MARRYVIRQDAAPVAPISGIDYRTALNEEQYAAVSSEPGPALVIAGAGSGKTRTLTYRVAWLLDHGTDPWNILLLTFTNK
AAREMTERVRSLIPLDLSRLWSGTFHSIANRILRQHAEYLGYTPAFTIMDSDDRKSMIKSVVKSLKLDEKSTRFPKPEVL
SSLFSLADNEGANIRETQENAYPYLSNHLDAILEVHDQYVARKQETNSMDFDDLLLNVVRLFQEQEHLRALYGSRFQHIL
VDEYQDTNYLQSRLIDMLAREHGQLMVVGDDAQSIYSWRGADMENILSFTTRYPAAKTFKIETNYRSVPEILELSNAAIA
ANEVQIEKQLRSVRPTGEMPPALVPLNDDRMQAKFVVQRIRDLIEEGTDPSEIAVLYRAHFHSMELQMELTRGEIPFRIT
SGIRFFEQAHIKDVVAFMRFVVNPRDELSFRRMVMLLPGIGPGMAQKLWIRWTACPESRQETPPESFSALMLDFPVPAKS
RTAWTQLCYTLDELAPAGKLAGPASMLLSINEAVYDEYMQAAFENYDQRRQDLLHLAGFSERFESTEDFLSQLSLLGNTD
DESAAADFSGGSVTLSTIHQAKGLEWSVVFLIGLCDGMFPHQRVIDDGDLAGLEEERRLFYVGITRAKDQLYLTYPRWNC
RAQGGTFMQMPSRFLDEFPAGLVEEWEVE

Sequences:

>Translated_669_residues
MARRYVIRQDAAPVAPISGIDYRTALNEEQYAAVSSEPGPALVIAGAGSGKTRTLTYRVAWLLDHGTDPWNILLLTFTNK
AAREMTERVRSLIPLDLSRLWSGTFHSIANRILRQHAEYLGYTPAFTIMDSDDRKSMIKSVVKSLKLDEKSTRFPKPEVL
SSLFSLADNEGANIRETQENAYPYLSNHLDAILEVHDQYVARKQETNSMDFDDLLLNVVRLFQEQEHLRALYGSRFQHIL
VDEYQDTNYLQSRLIDMLAREHGQLMVVGDDAQSIYSWRGADMENILSFTTRYPAAKTFKIETNYRSVPEILELSNAAIA
ANEVQIEKQLRSVRPTGEMPPALVPLNDDRMQAKFVVQRIRDLIEEGTDPSEIAVLYRAHFHSMELQMELTRGEIPFRIT
SGIRFFEQAHIKDVVAFMRFVVNPRDELSFRRMVMLLPGIGPGMAQKLWIRWTACPESRQETPPESFSALMLDFPVPAKS
RTAWTQLCYTLDELAPAGKLAGPASMLLSINEAVYDEYMQAAFENYDQRRQDLLHLAGFSERFESTEDFLSQLSLLGNTD
DESAAADFSGGSVTLSTIHQAKGLEWSVVFLIGLCDGMFPHQRVIDDGDLAGLEEERRLFYVGITRAKDQLYLTYPRWNC
RAQGGTFMQMPSRFLDEFPAGLVEEWEVE
>Mature_668_residues
ARRYVIRQDAAPVAPISGIDYRTALNEEQYAAVSSEPGPALVIAGAGSGKTRTLTYRVAWLLDHGTDPWNILLLTFTNKA
AREMTERVRSLIPLDLSRLWSGTFHSIANRILRQHAEYLGYTPAFTIMDSDDRKSMIKSVVKSLKLDEKSTRFPKPEVLS
SLFSLADNEGANIRETQENAYPYLSNHLDAILEVHDQYVARKQETNSMDFDDLLLNVVRLFQEQEHLRALYGSRFQHILV
DEYQDTNYLQSRLIDMLAREHGQLMVVGDDAQSIYSWRGADMENILSFTTRYPAAKTFKIETNYRSVPEILELSNAAIAA
NEVQIEKQLRSVRPTGEMPPALVPLNDDRMQAKFVVQRIRDLIEEGTDPSEIAVLYRAHFHSMELQMELTRGEIPFRITS
GIRFFEQAHIKDVVAFMRFVVNPRDELSFRRMVMLLPGIGPGMAQKLWIRWTACPESRQETPPESFSALMLDFPVPAKSR
TAWTQLCYTLDELAPAGKLAGPASMLLSINEAVYDEYMQAAFENYDQRRQDLLHLAGFSERFESTEDFLSQLSLLGNTDD
ESAAADFSGGSVTLSTIHQAKGLEWSVVFLIGLCDGMFPHQRVIDDGDLAGLEEERRLFYVGITRAKDQLYLTYPRWNCR
AQGGTFMQMPSRFLDEFPAGLVEEWEVE

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=654, Percent_Identity=35.7798165137615, Blast_Score=353, Evalue=2e-98,
Organism=Escherichia coli, GI48994965, Length=648, Percent_Identity=31.9444444444444, Blast_Score=272, Evalue=6e-74,
Organism=Escherichia coli, GI1787196, Length=397, Percent_Identity=26.9521410579345, Blast_Score=105, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=500, Percent_Identity=27.4, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324477, Length=675, Percent_Identity=23.8518518518519, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76123; Mature: 75992

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRYVIRQDAAPVAPISGIDYRTALNEEQYAAVSSEPGPALVIAGAGSGKTRTLTYRVA
CCCCEEEECCCCCCCCCCCCCHHHHCCCHHHHCCCCCCCCEEEEEECCCCCEEEEEEEEE
WLLDHGTDPWNILLLTFTNKAAREMTERVRSLIPLDLSRLWSGTFHSIANRILRQHAEYL
EEEECCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHC
GYTPAFTIMDSDDRKSMIKSVVKSLKLDEKSTRFPKPEVLSSLFSLADNEGANIRETQEN
CCCCEEEEECCCHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHC
AYPYLSNHLDAILEVHDQYVARKQETNSMDFDDLLLNVVRLFQEQEHLRALYGSRFQHIL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VDEYQDTNYLQSRLIDMLAREHGQLMVVGDDAQSIYSWRGADMENILSFTTRYPAAKTFK
HHCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCHHHHHHHHHCCCCCEEEE
IETNYRSVPEILELSNAAIAANEVQIEKQLRSVRPTGEMPPALVPLNDDRMQAKFVVQRI
EECCCCCHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHH
RDLIEEGTDPSEIAVLYRAHFHSMELQMELTRGEIPFRITSGIRFFEQAHIKDVVAFMRF
HHHHHCCCCHHHHHHHHHHHHHCEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHH
VVNPRDELSFRRMVMLLPGIGPGMAQKLWIRWTACPESRQETPPESFSALMLDFPVPAKS
HCCCHHHHHHHHHHHHCCCCCCCCHHEEEEEEECCCCCCCCCCCHHHHEEEEECCCCCCC
RTAWTQLCYTLDELAPAGKLAGPASMLLSINEAVYDEYMQAAFENYDQRRQDLLHLAGFS
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
ERFESTEDFLSQLSLLGNTDDESAAADFSGGSVTLSTIHQAKGLEWSVVFLIGLCDGMFP
HHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHCCCCC
HQRVIDDGDLAGLEEERRLFYVGITRAKDQLYLTYPRWNCRAQGGTFMQMPSRFLDEFPA
CHHCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCC
GLVEEWEVE
CHHHHCCCC
>Mature Secondary Structure 
ARRYVIRQDAAPVAPISGIDYRTALNEEQYAAVSSEPGPALVIAGAGSGKTRTLTYRVA
CCCEEEECCCCCCCCCCCCCHHHHCCCHHHHCCCCCCCCEEEEEECCCCCEEEEEEEEE
WLLDHGTDPWNILLLTFTNKAAREMTERVRSLIPLDLSRLWSGTFHSIANRILRQHAEYL
EEEECCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHC
GYTPAFTIMDSDDRKSMIKSVVKSLKLDEKSTRFPKPEVLSSLFSLADNEGANIRETQEN
CCCCEEEEECCCHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHC
AYPYLSNHLDAILEVHDQYVARKQETNSMDFDDLLLNVVRLFQEQEHLRALYGSRFQHIL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VDEYQDTNYLQSRLIDMLAREHGQLMVVGDDAQSIYSWRGADMENILSFTTRYPAAKTFK
HHCCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCHHHHHHHHHCCCCCEEEE
IETNYRSVPEILELSNAAIAANEVQIEKQLRSVRPTGEMPPALVPLNDDRMQAKFVVQRI
EECCCCCHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHH
RDLIEEGTDPSEIAVLYRAHFHSMELQMELTRGEIPFRITSGIRFFEQAHIKDVVAFMRF
HHHHHCCCCHHHHHHHHHHHHHCEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHH
VVNPRDELSFRRMVMLLPGIGPGMAQKLWIRWTACPESRQETPPESFSALMLDFPVPAKS
HCCCHHHHHHHHHHHHCCCCCCCCHHEEEEEEECCCCCCCCCCCHHHHEEEEECCCCCCC
RTAWTQLCYTLDELAPAGKLAGPASMLLSINEAVYDEYMQAAFENYDQRRQDLLHLAGFS
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
ERFESTEDFLSQLSLLGNTDDESAAADFSGGSVTLSTIHQAKGLEWSVVFLIGLCDGMFP
HHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHCCCCC
HQRVIDDGDLAGLEEERRLFYVGITRAKDQLYLTYPRWNCRAQGGTFMQMPSRFLDEFPA
CHHCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCC
GLVEEWEVE
CHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA