Click here to switch to the map view.

The map label for this gene is nth [H]

Identifier: 186476851

GI number: 186476851

Start: 2356197

End: 2356841

Strand: Direct

Name: nth [H]

Synonym: Bphy_2097

Alternate gene names: 186476851

Gene position: 2356197-2356841 (Clockwise)

Preceding gene: 186476850

Following gene: 186476852

Centisome position: 67.72

GC content: 61.55

Gene sequence:

>645_bases
ATGAACGCGACCAAACGACGTGCGATCTACGAGACGCTCCAAAGCCTGAATCCCCATCCGAAGACGGAACTGGAATACAC
AACGCCGTTCGAGTTGCTGATCGCCGTGATGTTGTCGGCGCAAGCAACGGACGTCTCCGTGAACAAGGCGATGCGCCGGA
TGTTCCCTGTCGCGAACACGCCGCAGAAGGTGTTCGACCTCGGCGAGGAGGGCGTTGCCGACTACATCAAGACGATCGGG
TTGTATCGCACGAAGGCGAAGAACGTGATCGCGACGTGCCGCATTCTGCTCGACCAGTACGCGGGCGAAGTGCCCGCCGA
TCGCGAGGCGCTCGAAAGCCTGCCCGGTGTCGGTCGCAAGACGGCGAACGTCGTGTTGAACACGGCGTTCGGACAGCCGA
CCATCGCGGTCGATACGCACATCTTTCGGGTTGCGAATCGAACGGGCCTTGCGCCCGGCAAGGACGTGCGCGCCGTCGAA
GTGGCGCTGGAAAAATTCACGCCCGCCGAGTTCCTGCAAGACGCGCATCACTGGTTAATCCTGCACGGACGCTATGTGTG
CAAGGCGCGCCGGCCGGAATGCTGGCATTGCGTGATCGAGCCGTTGTGCGAGTTCAAGCCGAAGACGCCGCCGCCGGATC
TTTAA

Upstream 100 bases:

>100_bases
AGTCGAAGCGCGCCGTCGCCGTCTAGGCATTGCCACAGACATTTCCGACTCAACGTCCAAGCAGAACGACAATCAATCCG
ACAAGCCCTGACTGCACCGC

Downstream 100 bases:

>100_bases
GCACGCTGCGCGGCGTTTCGTCGGGTTCGCGCGGCCGCGCCGTCCGCCTGCGTGCGATCGAGCCGGGGCGTAAAATGAAC
CGATATTGCGCACGCCGCCA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MNATKRRAIYETLQSLNPHPKTELEYTTPFELLIAVMLSAQATDVSVNKAMRRMFPVANTPQKVFDLGEEGVADYIKTIG
LYRTKAKNVIATCRILLDQYAGEVPADREALESLPGVGRKTANVVLNTAFGQPTIAVDTHIFRVANRTGLAPGKDVRAVE
VALEKFTPAEFLQDAHHWLILHGRYVCKARRPECWHCVIEPLCEFKPKTPPPDL

Sequences:

>Translated_214_residues
MNATKRRAIYETLQSLNPHPKTELEYTTPFELLIAVMLSAQATDVSVNKAMRRMFPVANTPQKVFDLGEEGVADYIKTIG
LYRTKAKNVIATCRILLDQYAGEVPADREALESLPGVGRKTANVVLNTAFGQPTIAVDTHIFRVANRTGLAPGKDVRAVE
VALEKFTPAEFLQDAHHWLILHGRYVCKARRPECWHCVIEPLCEFKPKTPPPDL
>Mature_214_residues
MNATKRRAIYETLQSLNPHPKTELEYTTPFELLIAVMLSAQATDVSVNKAMRRMFPVANTPQKVFDLGEEGVADYIKTIG
LYRTKAKNVIATCRILLDQYAGEVPADREALESLPGVGRKTANVVLNTAFGQPTIAVDTHIFRVANRTGLAPGKDVRAVE
VALEKFTPAEFLQDAHHWLILHGRYVCKARRPECWHCVIEPLCEFKPKTPPPDL

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.638418079096, Blast_Score=98, Evalue=5e-21,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=68.75, Blast_Score=298, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=33.8983050847458, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=27.5862068965517, Blast_Score=75, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=25.668449197861, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=32.7777777777778, Blast_Score=110, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23923; Mature: 23923

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATKRRAIYETLQSLNPHPKTELEYTTPFELLIAVMLSAQATDVSVNKAMRRMFPVANT
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC
PQKVFDLGEEGVADYIKTIGLYRTKAKNVIATCRILLDQYAGEVPADREALESLPGVGRK
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCC
TANVVLNTAFGQPTIAVDTHIFRVANRTGLAPGKDVRAVEVALEKFTPAEFLQDAHHWLI
HHHHEEECCCCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCEEE
LHGRYVCKARRPECWHCVIEPLCEFKPKTPPPDL
EECCEEECCCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNATKRRAIYETLQSLNPHPKTELEYTTPFELLIAVMLSAQATDVSVNKAMRRMFPVANT
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC
PQKVFDLGEEGVADYIKTIGLYRTKAKNVIATCRILLDQYAGEVPADREALESLPGVGRK
CHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCC
TANVVLNTAFGQPTIAVDTHIFRVANRTGLAPGKDVRAVEVALEKFTPAEFLQDAHHWLI
HHHHEEECCCCCCEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCEEE
LHGRYVCKARRPECWHCVIEPLCEFKPKTPPPDL
EECCEEECCCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]