| Definition | Burkholderia phymatum STM815 chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_010622 |
| Length | 3,479,187 |
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The map label for this gene is mrcA [H]
Identifier: 186476027
GI number: 186476027
Start: 1421150
End: 1423510
Strand: Reverse
Name: mrcA [H]
Synonym: Bphy_1268
Alternate gene names: 186476027
Gene position: 1423510-1421150 (Counterclockwise)
Preceding gene: 186476031
Following gene: 186476023
Centisome position: 40.92
GC content: 64.89
Gene sequence:
>2361_bases ATGTGGTTCGCTGGCCTGTTCGCCACGCTCGCCGTGGTGGGGGCGCTGATCGTCGGCTACGCGCTCGTCGTGATGGGCCC GCAGTTGCCGTCGCTCGACGCGCTGACCGACTATCGCCCGAAGGTCCCGCTGCGCATCTACACGGCCGACCACGTGCTGA TCGGCGAGTTCGGCGAAGAGCGGCGCAGCCTCGTGCGCTTTCAGGACATCCCCGACGTGCAGAAGAAGGCTGTGCTCGCG ATCGAGGACTACCGTTTCTACGAGCACGGCGGCGTCGATTTCATCGGCATTCTGCGCGCGGGTTTCGCGGACATCATGCA CGGCGGCTCGTCGCAGGGCGCAAGCACGATCACGATGCAGGTCGCGCGTAATTTCTTTCTGTCGAGTGAAAAGACGTACA CCCGCAAGGTCTATGAAATGCTGCTCGCGTACAAGATCGAGCGCGCGCTCACCAAAGACCAGATTCTGGAGCTGTACATG AACCAGATCTATCTGGGCGAACGCGCTTACGGCTTCGCGGCGGCGGCACGTGTGTACTTCGGCAAGGATCTGAAAGACAT CACGCTCGCCGAAGCGGCGATGCTCGCGGGGCTGCCGAAGGCGCCGTCCGCGTACAACCCGGTCGTCAATCCGAAGCGCG CGAAGATCCGACAGGAATACATCCTCAAGCGCATGCTGGACCTGAATTACATTACGCAGGACCAGTACAACCAGGCCGTG AAGGAAGAGATTCGCACGAAGACGGCGGGCAACGAGTACAGCGTGCACGCCGAGTACATCGCGGAAATGGTGCGTCAGAT GATGTACGCGCAGTACAAGGACGAGACGTACACGCGTGGCCTCAACGTGACGACGACGATCGATTCGGCCGATCAGGAAG CCGCATATCGCGCGGTGCGCAAAGGCGTGATGGACTACGAGCGCCGCCACGGCTATCGCGGACCGGAAGGCTTCGTCGAA TTGCCGGCTGCTGGCGACGAGCGCGACGAGGCGATCGAGGACGCGCTGAACGATCATCCCGACAACGGCGAGATCGTGGC GGCCGTCGTCACGTCGGCGACCGCGAAGGAAGTGAAGGCTCAAATGCTCGACGGCACGCAGGCAAGCGTGACGGGCGACG GCCTGCGCTTTGCAGCCGGTTCGCTGTCGGCGCGCGCCGCGCAGGCGCAGAAGATCCGGCCTGGCTCGATCGTGCGGCTG GTGGCCGATGCGAACGGCAAATGGCAGATCACGCAGTTGCCGCAGGTGGAGGGCGCGCTGGTGTCGATGACGCCGCAGGA CGGCGCGATTCGCTCGCTCGTCGGCGGCTTCGACTTCAACAAGAACAAGTTCAACCATGTGACGCAGGCATGGCGTCAGC CGGGTTCGAGCTTTAAACCGTTCATCTATTCGGCTGCGCTCGACAAGGGCCTTGGACCGGCGACGATCATCAACGACGCG CCGCTCTACTTCCCGCCGAGCGCGCCGGGCGGCGACGCGTGGGAGCCAAAGGACGACGACCAGCCCGACGGTCCGATGCC CATGCGTCTTGCGCTGCAGAAGTCGAAGAACCTGGTGTCGATCCGCATCCTGTCCTATATCGGCACGAAGTACGCGCAGG ACTTCGTCACGCAGCGCTTCGGTTTCGACGCCGACAAGACGCCGCCATACTTGCCGATGGCGCTCGGCGCGGGCCTCGTC ACGCCGCTGCAGTCGGCGGGCGCGTATAGCGTGTTCGCGAACGGCGGCTACCGCATCAATCCGTATCTGATCGCCGAAGT CGACGATCCGCATGGGCAGCCGCTGTCGCGCGCGCAGCCGTTGACGGCGGGCCGCGACGCGCCGCGCACGCTCGAGCCGC GCAACGCGTACATCATGAACAGCCTGCTGCATTCGGTGGCGACGGCGGGTACGGGCGCGGGCACCAACGTGCTGCATCGA AGCGACCTGCAGGGCAAGACGGGGACCACGAACGACGCGAAGGACGGCTGGTTCGCCGGCTATCAGCAGTCGCTCGTGGC CGTCGCGTGGATGGGTTACGACCAGCCGAAGTCGCTTGGCAGCCGTGAATTCGGCGCGCAACTGGCCCTCCCGATCTGGG TCGAGTACATGCAGCGCGCGCTGCGCGGCGTGCCGCAAGCCGAGCCGGAGATGCCGCCGGGCGTGACGACCGTCGATGGC GAACTGTTCTATGCGGACATGACGCCGGGCGCCGGGTTCGTCGCGAGCATCGGCATGGATTCGGCGAATCCGCTGGCGAG CGGCGGCGATGCCGTCGGTACGGTGGGGCCCGCAGGGATGACACCGCCCGCGCCGCCGCCGAACGTCACGTCGACGGAGA AGAAGCAGATCATGGATCTGTTCGAGTCGAACAAGCCTTGA
Upstream 100 bases:
>100_bases GAGCCGCGACTATCGCTACGCCCAGCGTGAAGATGACGCCGGCGATGAGCGCGGCCGTGGCAGGCGCTCCGGGGGACGCT CAATAGGCGGCACGATCGCG
Downstream 100 bases:
>100_bases TGGTTTGAGCCTTCGACGCCGGCAAACACGCGCCCGTGGTGGACATCACGGGCGCGTTTTTTTGTGCAGTCGATGGAGGC ATGCGCGCCAAGCGATGATT
Product: 1A family penicillin-binding protein
Products: NA
Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 786; Mature: 786
Protein sequence:
>786_residues MWFAGLFATLAVVGALIVGYALVVMGPQLPSLDALTDYRPKVPLRIYTADHVLIGEFGEERRSLVRFQDIPDVQKKAVLA IEDYRFYEHGGVDFIGILRAGFADIMHGGSSQGASTITMQVARNFFLSSEKTYTRKVYEMLLAYKIERALTKDQILELYM NQIYLGERAYGFAAAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKIRQEYILKRMLDLNYITQDQYNQAV KEEIRTKTAGNEYSVHAEYIAEMVRQMMYAQYKDETYTRGLNVTTTIDSADQEAAYRAVRKGVMDYERRHGYRGPEGFVE LPAAGDERDEAIEDALNDHPDNGEIVAAVVTSATAKEVKAQMLDGTQASVTGDGLRFAAGSLSARAAQAQKIRPGSIVRL VADANGKWQITQLPQVEGALVSMTPQDGAIRSLVGGFDFNKNKFNHVTQAWRQPGSSFKPFIYSAALDKGLGPATIINDA PLYFPPSAPGGDAWEPKDDDQPDGPMPMRLALQKSKNLVSIRILSYIGTKYAQDFVTQRFGFDADKTPPYLPMALGAGLV TPLQSAGAYSVFANGGYRINPYLIAEVDDPHGQPLSRAQPLTAGRDAPRTLEPRNAYIMNSLLHSVATAGTGAGTNVLHR SDLQGKTGTTNDAKDGWFAGYQQSLVAVAWMGYDQPKSLGSREFGAQLALPIWVEYMQRALRGVPQAEPEMPPGVTTVDG ELFYADMTPGAGFVASIGMDSANPLASGGDAVGTVGPAGMTPPAPPPNVTSTEKKQIMDLFESNKP
Sequences:
>Translated_786_residues MWFAGLFATLAVVGALIVGYALVVMGPQLPSLDALTDYRPKVPLRIYTADHVLIGEFGEERRSLVRFQDIPDVQKKAVLA IEDYRFYEHGGVDFIGILRAGFADIMHGGSSQGASTITMQVARNFFLSSEKTYTRKVYEMLLAYKIERALTKDQILELYM NQIYLGERAYGFAAAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKIRQEYILKRMLDLNYITQDQYNQAV KEEIRTKTAGNEYSVHAEYIAEMVRQMMYAQYKDETYTRGLNVTTTIDSADQEAAYRAVRKGVMDYERRHGYRGPEGFVE LPAAGDERDEAIEDALNDHPDNGEIVAAVVTSATAKEVKAQMLDGTQASVTGDGLRFAAGSLSARAAQAQKIRPGSIVRL VADANGKWQITQLPQVEGALVSMTPQDGAIRSLVGGFDFNKNKFNHVTQAWRQPGSSFKPFIYSAALDKGLGPATIINDA PLYFPPSAPGGDAWEPKDDDQPDGPMPMRLALQKSKNLVSIRILSYIGTKYAQDFVTQRFGFDADKTPPYLPMALGAGLV TPLQSAGAYSVFANGGYRINPYLIAEVDDPHGQPLSRAQPLTAGRDAPRTLEPRNAYIMNSLLHSVATAGTGAGTNVLHR SDLQGKTGTTNDAKDGWFAGYQQSLVAVAWMGYDQPKSLGSREFGAQLALPIWVEYMQRALRGVPQAEPEMPPGVTTVDG ELFYADMTPGAGFVASIGMDSANPLASGGDAVGTVGPAGMTPPAPPPNVTSTEKKQIMDLFESNKP >Mature_786_residues MWFAGLFATLAVVGALIVGYALVVMGPQLPSLDALTDYRPKVPLRIYTADHVLIGEFGEERRSLVRFQDIPDVQKKAVLA IEDYRFYEHGGVDFIGILRAGFADIMHGGSSQGASTITMQVARNFFLSSEKTYTRKVYEMLLAYKIERALTKDQILELYM NQIYLGERAYGFAAAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKIRQEYILKRMLDLNYITQDQYNQAV KEEIRTKTAGNEYSVHAEYIAEMVRQMMYAQYKDETYTRGLNVTTTIDSADQEAAYRAVRKGVMDYERRHGYRGPEGFVE LPAAGDERDEAIEDALNDHPDNGEIVAAVVTSATAKEVKAQMLDGTQASVTGDGLRFAAGSLSARAAQAQKIRPGSIVRL VADANGKWQITQLPQVEGALVSMTPQDGAIRSLVGGFDFNKNKFNHVTQAWRQPGSSFKPFIYSAALDKGLGPATIINDA PLYFPPSAPGGDAWEPKDDDQPDGPMPMRLALQKSKNLVSIRILSYIGTKYAQDFVTQRFGFDADKTPPYLPMALGAGLV TPLQSAGAYSVFANGGYRINPYLIAEVDDPHGQPLSRAQPLTAGRDAPRTLEPRNAYIMNSLLHSVATAGTGAGTNVLHR SDLQGKTGTTNDAKDGWFAGYQQSLVAVAWMGYDQPKSLGSREFGAQLALPIWVEYMQRALRGVPQAEPEMPPGVTTVDG ELFYADMTPGAGFVASIGMDSANPLASGGDAVGTVGPAGMTPPAPPPNVTSTEKKQIMDLFESNKP
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG5009
COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI87082258, Length=843, Percent_Identity=38.1969157769869, Blast_Score=526, Evalue=1e-150, Organism=Escherichia coli, GI1786343, Length=249, Percent_Identity=36.144578313253, Blast_Score=146, Evalue=6e-36, Organism=Escherichia coli, GI1788867, Length=232, Percent_Identity=31.0344827586207, Blast_Score=113, Evalue=4e-26, Organism=Escherichia coli, GI1789601, Length=164, Percent_Identity=34.1463414634146, Blast_Score=87, Evalue=3e-18,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011816 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.2.-; 3.4.-.- [C]
Molecular weight: Translated: 85587; Mature: 85587
Theoretical pI: Translated: 5.91; Mature: 5.91
Prosite motif: PS00639 THIOL_PROTEASE_HIS
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 3.2 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 3.2 %Met (Mature Protein) 3.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MWFAGLFATLAVVGALIVGYALVVMGPQLPSLDALTDYRPKVPLRIYTADHVLIGEFGEE CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEECCEEEECCCCHH RRSLVRFQDIPDVQKKAVLAIEDYRFYEHGGVDFIGILRAGFADIMHGGSSQGASTITMQ HHHHHHHCCCCCHHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHH VARNFFLSSEKTYTRKVYEMLLAYKIERALTKDQILELYMNQIYLGERAYGFAAAARVYF HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH GKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKIRQEYILKRMLDLNYITQDQYNQAV CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH KEEIRTKTAGNEYSVHAEYIAEMVRQMMYAQYKDETYTRGLNVTTTIDSADQEAAYRAVR HHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCHHHHHHHHHH KGVMDYERRHGYRGPEGFVELPAAGDERDEAIEDALNDHPDNGEIVAAVVTSATAKEVKA HHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHH QMLDGTQASVTGDGLRFAAGSLSARAAQAQKIRPGSIVRLVADANGKWQITQLPQVEGAL HHHCCCCCEECCCCCEECCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCEE VSMTPQDGAIRSLVGGFDFNKNKFNHVTQAWRQPGSSFKPFIYSAALDKGLGPATIINDA EEECCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEECCC PLYFPPSAPGGDAWEPKDDDQPDGPMPMRLALQKSKNLVSIRILSYIGTKYAQDFVTQRF CEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHC GFDADKTPPYLPMALGAGLVTPLQSAGAYSVFANGGYRINPYLIAEVDDPHGQPLSRAQP CCCCCCCCCCCHHHHCCCHHHHHHCCCCEEEEECCCEEECCEEEEEECCCCCCCCCCCCC LTAGRDAPRTLEPRNAYIMNSLLHSVATAGTGAGTNVLHRSDLQGKTGTTNDAKDGWFAG CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHH YQQSLVAVAWMGYDQPKSLGSREFGAQLALPIWVEYMQRALRGVPQAEPEMPPGVTTVDG HHHHHHHHEECCCCCCHHCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECC ELFYADMTPGAGFVASIGMDSANPLASGGDAVGTVGPAGMTPPAPPPNVTSTEKKQIMDL EEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH FESNKP HHCCCC >Mature Secondary Structure MWFAGLFATLAVVGALIVGYALVVMGPQLPSLDALTDYRPKVPLRIYTADHVLIGEFGEE CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEECCEEEECCCCHH RRSLVRFQDIPDVQKKAVLAIEDYRFYEHGGVDFIGILRAGFADIMHGGSSQGASTITMQ HHHHHHHCCCCCHHHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHH VARNFFLSSEKTYTRKVYEMLLAYKIERALTKDQILELYMNQIYLGERAYGFAAAARVYF HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH GKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKRAKIRQEYILKRMLDLNYITQDQYNQAV CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH KEEIRTKTAGNEYSVHAEYIAEMVRQMMYAQYKDETYTRGLNVTTTIDSADQEAAYRAVR HHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCHHHHHHHHHH KGVMDYERRHGYRGPEGFVELPAAGDERDEAIEDALNDHPDNGEIVAAVVTSATAKEVKA HHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHH QMLDGTQASVTGDGLRFAAGSLSARAAQAQKIRPGSIVRLVADANGKWQITQLPQVEGAL HHHCCCCCEECCCCCEECCCCHHHHHHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCEE VSMTPQDGAIRSLVGGFDFNKNKFNHVTQAWRQPGSSFKPFIYSAALDKGLGPATIINDA EEECCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEECCC PLYFPPSAPGGDAWEPKDDDQPDGPMPMRLALQKSKNLVSIRILSYIGTKYAQDFVTQRF CEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHC GFDADKTPPYLPMALGAGLVTPLQSAGAYSVFANGGYRINPYLIAEVDDPHGQPLSRAQP CCCCCCCCCCCHHHHCCCHHHHHHCCCCEEEEECCCEEECCEEEEEECCCCCCCCCCCCC LTAGRDAPRTLEPRNAYIMNSLLHSVATAGTGAGTNVLHRSDLQGKTGTTNDAKDGWFAG CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHH YQQSLVAVAWMGYDQPKSLGSREFGAQLALPIWVEYMQRALRGVPQAEPEMPPGVTTVDG HHHHHHHHEECCCCCCHHCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECC ELFYADMTPGAGFVASIGMDSANPLASGGDAVGTVGPAGMTPPAPPPNVTSTEKKQIMDL EEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH FESNKP HHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: NA