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The map label for this gene is ycaL [H]

Identifier: 183222605

GI number: 183222605

Start: 3373828

End: 3374844

Strand: Direct

Name: ycaL [H]

Synonym: LEPBI_I3260

Alternate gene names: 183222605

Gene position: 3373828-3374844 (Clockwise)

Preceding gene: 183222604

Following gene: 183222607

Centisome position: 93.73

GC content: 36.97

Gene sequence:

>1017_bases
TTGATTCTTCTTCCAGACGAAACCAAAGAAAGTCCAGTTTTGGAAATTTTTTGTACAAAAGCAGAATCCAAACTATTAGA
AAAAATTTGGATCCAAAACAAAAAATCACAAAGTCATTCCAGTGCTCTTTTTTACTCCATTCGCGAAATGAACCCGATCG
TTCTTGGAATCCTATCGCTTACGGTAGTTTCCATCGTTGGTTTTTTCTATTTTAAAGGACTAGAACTCGTTACCAATGTC
ATTCCTCTTTCGATGGACAAATCGTTGGGAGATTCCGTTCAATTGAAAATGGAATCGCAATTTGAAAAATGTGATTCAAA
AGCAACGGATCGTTTTTTCGCAGAAGCACTTAAAAAAATAGTTCCAAAAGATAGCAAACATGAGTTTCAAGTTTCGGTGA
TTGCTTCCACCGTTCCCAATGCCTTTGCCTTATCCAATGGTAAAATTTATTTTTTCTCAGGATTACTGAACGAAGCCGAG
TCACAAGAAGAGGTGATAGGTGTGTTAGCACATGAAATTGCCCATGTGGAAAAAAGACACCATATGCGGAATTTGGTCAA
AGCTGGTGGAACTTCGCTTGCGATCAGTTTGGTGGTAGGTCCTGGTTTAGGGAATATGGAATTTTTAGAAACCTTCACAG
AAATTGGTTCCACTATTTTGGTATTGAAGTTTTCCAGAGACTTTGAAACAGAAGCGGACAAAACTTCTATCGAATATCTA
AAAGCACAAAATATGTCTACAGATGGACTCCTTAGTTTTTTCAAACGAATGGAAACATTGGAAAATGATGAAACCGAATC
GGATCAATCTGCTACCAGTGCGAAAGAAGGTGACTCTACTGTTAGTCAAATCACAGATTTTTTAAGTACGCACCCCGCGA
CAAAAGAAAGGATGAAAACATTAGAGACTTTGATTCAGAAAGGTAAAAAAGGGAACATCAAAAAAGTGGTTTCTGATAAG
ACTTGGAATGAAGTTCAAACGGTTTGTTTGAATTTAAAAAATTCGGATTCCAAATGA

Upstream 100 bases:

>100_bases
TGTCCATGGCCAAACTGTCCAATTCACATCATCTGATACTCATCATAAATTAAATATTTCCCAATTTACAGAATTCACAC
AGACGCATAAGGGCTGTAAG

Downstream 100 bases:

>100_bases
TCATAAATTTCCTTTAGAGTTTCACTCAAACTATGTTTGTTTTTCCACCCTAACGATTTTAGTTTTTGATTGTTACCATA
CACCCGAGATGTTTCGGAGG

Product: putative metalloendopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MILLPDETKESPVLEIFCTKAESKLLEKIWIQNKKSQSHSSALFYSIREMNPIVLGILSLTVVSIVGFFYFKGLELVTNV
IPLSMDKSLGDSVQLKMESQFEKCDSKATDRFFAEALKKIVPKDSKHEFQVSVIASTVPNAFALSNGKIYFFSGLLNEAE
SQEEVIGVLAHEIAHVEKRHHMRNLVKAGGTSLAISLVVGPGLGNMEFLETFTEIGSTILVLKFSRDFETEADKTSIEYL
KAQNMSTDGLLSFFKRMETLENDETESDQSATSAKEGDSTVSQITDFLSTHPATKERMKTLETLIQKGKKGNIKKVVSDK
TWNEVQTVCLNLKNSDSK

Sequences:

>Translated_338_residues
MILLPDETKESPVLEIFCTKAESKLLEKIWIQNKKSQSHSSALFYSIREMNPIVLGILSLTVVSIVGFFYFKGLELVTNV
IPLSMDKSLGDSVQLKMESQFEKCDSKATDRFFAEALKKIVPKDSKHEFQVSVIASTVPNAFALSNGKIYFFSGLLNEAE
SQEEVIGVLAHEIAHVEKRHHMRNLVKAGGTSLAISLVVGPGLGNMEFLETFTEIGSTILVLKFSRDFETEADKTSIEYL
KAQNMSTDGLLSFFKRMETLENDETESDQSATSAKEGDSTVSQITDFLSTHPATKERMKTLETLIQKGKKGNIKKVVSDK
TWNEVQTVCLNLKNSDSK
>Mature_338_residues
MILLPDETKESPVLEIFCTKAESKLLEKIWIQNKKSQSHSSALFYSIREMNPIVLGILSLTVVSIVGFFYFKGLELVTNV
IPLSMDKSLGDSVQLKMESQFEKCDSKATDRFFAEALKKIVPKDSKHEFQVSVIASTVPNAFALSNGKIYFFSGLLNEAE
SQEEVIGVLAHEIAHVEKRHHMRNLVKAGGTSLAISLVVGPGLGNMEFLETFTEIGSTILVLKFSRDFETEADKTSIEYL
KAQNMSTDGLLSFFKRMETLENDETESDQSATSAKEGDSTVSQITDFLSTHPATKERMKTLETLIQKGKKGNIKKVVSDK
TWNEVQTVCLNLKNSDSK

Specific function: Unknown

COG id: COG4783

COG function: function code R; Putative Zn-dependent protease, contains TPR repeats

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M48B family [H]

Homologues:

Organism=Escherichia coli, GI87081800, Length=175, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322940, Length=188, Percent_Identity=28.7234042553192, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001915 [H]

Pfam domain/function: PF01435 Peptidase_M48 [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 37653; Mature: 37653

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILLPDETKESPVLEIFCTKAESKLLEKIWIQNKKSQSHSSALFYSIREMNPIVLGILSL
CEECCCCCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
TVVSIVGFFYFKGLELVTNVIPLSMDKSLGDSVQLKMESQFEKCDSKATDRFFAEALKKI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VPKDSKHEFQVSVIASTVPNAFALSNGKIYFFSGLLNEAESQEEVIGVLAHEIAHVEKRH
CCCCCCCCEEEEEEHHHCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HMRNLVKAGGTSLAISLVVGPGLGNMEFLETFTEIGSTILVLKFSRDFETEADKTSIEYL
HHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHH
KAQNMSTDGLLSFFKRMETLENDETESDQSATSAKEGDSTVSQITDFLSTHPATKERMKT
HHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHH
LETLIQKGKKGNIKKVVSDKTWNEVQTVCLNLKNSDSK
HHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MILLPDETKESPVLEIFCTKAESKLLEKIWIQNKKSQSHSSALFYSIREMNPIVLGILSL
CEECCCCCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
TVVSIVGFFYFKGLELVTNVIPLSMDKSLGDSVQLKMESQFEKCDSKATDRFFAEALKKI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
VPKDSKHEFQVSVIASTVPNAFALSNGKIYFFSGLLNEAESQEEVIGVLAHEIAHVEKRH
CCCCCCCCEEEEEEHHHCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HMRNLVKAGGTSLAISLVVGPGLGNMEFLETFTEIGSTILVLKFSRDFETEADKTSIEYL
HHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHH
KAQNMSTDGLLSFFKRMETLENDETESDQSATSAKEGDSTVSQITDFLSTHPATKERMKT
HHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHH
LETLIQKGKKGNIKKVVSDKTWNEVQTVCLNLKNSDSK
HHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7836281 [H]