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The map label for this gene is rsbW [H]

Identifier: 183221727

GI number: 183221727

Start: 2426273

End: 2426704

Strand: Reverse

Name: rsbW [H]

Synonym: LEPBI_I2356

Alternate gene names: 183221727

Gene position: 2426704-2426273 (Counterclockwise)

Preceding gene: 183221728

Following gene: 183221726

Centisome position: 67.41

GC content: 37.04

Gene sequence:

>432_bases
ATGGTAAACCCTTCAAAACATGCGGACTACGGCAAAGTAGTCCGTATCCAAATTCCTTCAAACCCCAGATTTATTTCCCA
CACTCGCAATTATTTTTTCCATCTATGTTTAGAACATGGATTTTCATTATTTGATTCCATGGACTTAAAACTTGTGATTG
GTGAAGCCATCACCAATATCATTCGGCATGCCTATTCTGGTCAGACAAATAAACCCATTTTCATTGAAATCCAATTTGAT
AAAGACAGAGTCGAAATTAAACTCAGGGATTACGGCCTAAAAGTAGAACCAAAAGACCTACGTAGTTTTGATTTGAGTGA
TTACAGAGAACATGGCATTGGTCTTTTTATGATCCGCGAACTCACCGATTATTATTTTTTAGACCAATCCTTCGAGATTG
GAAACCAGATGGTCCTCATCAAAAGAAAGTAA

Upstream 100 bases:

>100_bases
AGCTTCTCGGCCATATCGATATTTCGACGACACAGATTTATACCCATATGGCAAATAAAACTCTGAAGGAAGTTCATAAG
AAATTCCATCCGAGAGGATA

Downstream 100 bases:

>100_bases
CCTCCATTTTTCTCGACAAAGCTTCCTCATTTGGAAATTTATCCTTATGGAAACAATTCACGCAACCACCATCCTATCCG
TACGTAAAAACGGAAAAATC

Product: putative kinase

Products: NA

Alternate protein names: Anti-sigma-B factor; Sigma-B negative effector rsbW [H]

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MVNPSKHADYGKVVRIQIPSNPRFISHTRNYFFHLCLEHGFSLFDSMDLKLVIGEAITNIIRHAYSGQTNKPIFIEIQFD
KDRVEIKLRDYGLKVEPKDLRSFDLSDYREHGIGLFMIRELTDYYFLDQSFEIGNQMVLIKRK

Sequences:

>Translated_143_residues
MVNPSKHADYGKVVRIQIPSNPRFISHTRNYFFHLCLEHGFSLFDSMDLKLVIGEAITNIIRHAYSGQTNKPIFIEIQFD
KDRVEIKLRDYGLKVEPKDLRSFDLSDYREHGIGLFMIRELTDYYFLDQSFEIGNQMVLIKRK
>Mature_143_residues
MVNPSKHADYGKVVRIQIPSNPRFISHTRNYFFHLCLEHGFSLFDSMDLKLVIGEAITNIIRHAYSGQTNKPIFIEIQFD
KDRVEIKLRDYGLKVEPKDLRSFDLSDYREHGIGLFMIRELTDYYFLDQSFEIGNQMVLIKRK

Specific function: Negative regulator of sigma-B activity. Phosphorylates and inactivates its specific antagonist protein, rsbV. Upon phosphorylation of rsbV, rsbW is released and binds to sigma-B, thereby blocking its ability to form an RNA polymerase holoenzyme (E-sigma-B

COG id: COG2172

COG function: function code T; Anti-sigma regulatory factor (Ser/Thr protein kinase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anti-sigma-factor family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010193
- InterPro:   IPR003594 [H]

Pfam domain/function: PF02518 HATPase_c [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 16904; Mature: 16904

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNPSKHADYGKVVRIQIPSNPRFISHTRNYFFHLCLEHGFSLFDSMDLKLVIGEAITNI
CCCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHCCCHHHCCCCEEEEHHHHHHHH
IRHAYSGQTNKPIFIEIQFDKDRVEIKLRDYGLKVEPKDLRSFDLSDYREHGIGLFMIRE
HHHHHCCCCCCCEEEEEEECCCEEEEEEECCCCEECHHHCCCCCCCHHHHCCCEEEEEEH
LTDYYFLDQSFEIGNQMVLIKRK
HHHHHEECCCCCCCCEEEEEEEC
>Mature Secondary Structure
MVNPSKHADYGKVVRIQIPSNPRFISHTRNYFFHLCLEHGFSLFDSMDLKLVIGEAITNI
CCCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHCCCHHHCCCCEEEEHHHHHHHH
IRHAYSGQTNKPIFIEIQFDKDRVEIKLRDYGLKVEPKDLRSFDLSDYREHGIGLFMIRE
HHHHHCCCCCCCEEEEEEECCCEEEEEEECCCCEECHHHCCCCCCCHHHHCCCEEEEEEH
LTDYYFLDQSFEIGNQMVLIKRK
HHHHHEECCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]