Click here to switch to the map view.

The map label for this gene is ptsI [H]

Identifier: 183221253

GI number: 183221253

Start: 1959516

End: 1961243

Strand: Direct

Name: ptsI [H]

Synonym: LEPBI_I1869

Alternate gene names: 183221253

Gene position: 1959516-1961243 (Clockwise)

Preceding gene: 183221252

Following gene: 183221256

Centisome position: 54.44

GC content: 42.13

Gene sequence:

>1728_bases
ATGGAAGAAAAAACCACATTTAAAGGCATCTCTGCCTACCCAGGAACAGTCTACGGGAAAGTATTTCGTTGGAAACAAAC
CAAACGGAAACGGGAGGATCGTACAGACCTATCTCCTGAGGAAATCAAAGAGGAAGTGGAACTCTTAAAAAAAGGCCTTC
AAAAAACCGAAGATGACCTGGCAGACCTTGTCCAAAAATCCAAACAAAACCAAGAACTTTCGGAAATCTTAGAATCCCAA
ATTGTTTTTTTGAACGACCCCCTCTTTCGAGCTCGTGTCTTTGAAAGGATCGCACAAAACAATGAATCGGCGGGACTTGC
CTTGGAAACAGCAGTGAGTTCCCTCTATGACGAATTCCAATCCATCCCCGATGAATTCTTTCGAGAACGCGCCGACCACC
TTCTCGATATCGGCAAACGCATTGAATCCAATTTGTATCCCGAAAAAGGATCAGAGCCTACAAAGATTCCAGACGATGTC
ATCCTCATTGCCAAAGAGATCACCCCTTCCGAGATGATCCAATTGGGGAAAAGTAAGTTACGAGGGATTGCTACAGACTT
CGGTGGGAAAACAGGTCACACTGCCATCATTGCTAGAAATTATGGAATCCCAACCATCGTTGGATTGAAAAACATCACCT
CACACGTGGAAGATGATGATTATATCTTACTGGATGCCACAAAAGGAGTTCTCAATCGTTCCCCAGGAATCGAAGAAATC
AAACTGGCTGGGATACGAAGTGATATCAAAAAAATAATCCCTGTTCGGGAGATCAGTGACGGTCCAAAAGAACTCAAAAC
AAAAGATGGCAAAACCTTTACCTTACGAGCCAATATCGATTCGGAAGAAGAAGTGGACGGAGCCTTCCTGCAAGGGGCCG
ATGGAATTGGACTCGTTCGTACTGAAATTTTATTCATTCGGTATGTGGAATTCAAACCTACCGAAGAAGAACAGTTTGCA
GTTTACAAACGAATTTTACTCAAAATGGTCGGCCGACCTGTTACCTTTCGGGTTTGGGACATTGGTGCCGATAAAATGGA
AAATGGATACGAAGAAGAAAATCCATTTCTTGGAAACCGTGGGATCCGTTACCTCCTCCGCCACCCACATTTTTTTAAAG
AACAATTGAGAGCCCTATTACGTGCGAGTGAATATGGAACCATGCGCATCATGTTACCGATGATCACAACTCGTTCCGAA
ATTTTACAAACAAAGGTTTTATTAAATGAATGTTTGGATGAGTTAAAACAAACAGGACTTTTCATTACTAAAAAAATCCC
CTTAGGGATCATGGTGGAAACACCTGCCTGTGCTCTCAATCTTCCCTTTTTAGGAAACCATGTAGACTTTTATAGCGTTG
GTACAAACGATTTATTACAATACCTACTTGCCGTGGAACGTAACAACCATTTGGTGGGAGACCTTTACAACCCTTGGCAA
GTTGTGTTTTTATTATTACTGAAAAACATTGTGGAAGTGGCAAACTCCCAAAAAAAACCAATCAGCATTTGTGGTGAAAT
TGGAAGTGATCCTATGTTCACTGCAGTTCTCATTGGTCTTGGGTTTCGTGACCTAAGTTCTGCCTTACCACTCATGAAGG
ATGTAGCTGAAAAAGTCTCGGAAATCTCCACCTGGAAGGCAAAGTTATTAGCAGAACAAGTGATTTCCCTCGCAGGCGAA
GAAAAGTTCGATGAGATTGAAAAACTTGTTTTAGAAACCAAAGGTTAG

Upstream 100 bases:

>100_bases
GGATCCCACTTTTTTCCAAAAAAGAAGCCGCAAAGTAATCAAAACCGTTTTTTACTTTCCAAACCAGGCCAAAACAAAAG
TATTTTTGGCATAGGTTCGG

Downstream 100 bases:

>100_bases
TTGACCACCGAAGGAGTTGGGTTACGATTCCACCGATGCTCGTATGGGGGAACCATCGTCGTCCTTCGTTTGGTTCGTAT
CATTGATTAACCATAAGATG

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MEEKTTFKGISAYPGTVYGKVFRWKQTKRKREDRTDLSPEEIKEEVELLKKGLQKTEDDLADLVQKSKQNQELSEILESQ
IVFLNDPLFRARVFERIAQNNESAGLALETAVSSLYDEFQSIPDEFFRERADHLLDIGKRIESNLYPEKGSEPTKIPDDV
ILIAKEITPSEMIQLGKSKLRGIATDFGGKTGHTAIIARNYGIPTIVGLKNITSHVEDDDYILLDATKGVLNRSPGIEEI
KLAGIRSDIKKIIPVREISDGPKELKTKDGKTFTLRANIDSEEEVDGAFLQGADGIGLVRTEILFIRYVEFKPTEEEQFA
VYKRILLKMVGRPVTFRVWDIGADKMENGYEEENPFLGNRGIRYLLRHPHFFKEQLRALLRASEYGTMRIMLPMITTRSE
ILQTKVLLNECLDELKQTGLFITKKIPLGIMVETPACALNLPFLGNHVDFYSVGTNDLLQYLLAVERNNHLVGDLYNPWQ
VVFLLLLKNIVEVANSQKKPISICGEIGSDPMFTAVLIGLGFRDLSSALPLMKDVAEKVSEISTWKAKLLAEQVISLAGE
EKFDEIEKLVLETKG

Sequences:

>Translated_575_residues
MEEKTTFKGISAYPGTVYGKVFRWKQTKRKREDRTDLSPEEIKEEVELLKKGLQKTEDDLADLVQKSKQNQELSEILESQ
IVFLNDPLFRARVFERIAQNNESAGLALETAVSSLYDEFQSIPDEFFRERADHLLDIGKRIESNLYPEKGSEPTKIPDDV
ILIAKEITPSEMIQLGKSKLRGIATDFGGKTGHTAIIARNYGIPTIVGLKNITSHVEDDDYILLDATKGVLNRSPGIEEI
KLAGIRSDIKKIIPVREISDGPKELKTKDGKTFTLRANIDSEEEVDGAFLQGADGIGLVRTEILFIRYVEFKPTEEEQFA
VYKRILLKMVGRPVTFRVWDIGADKMENGYEEENPFLGNRGIRYLLRHPHFFKEQLRALLRASEYGTMRIMLPMITTRSE
ILQTKVLLNECLDELKQTGLFITKKIPLGIMVETPACALNLPFLGNHVDFYSVGTNDLLQYLLAVERNNHLVGDLYNPWQ
VVFLLLLKNIVEVANSQKKPISICGEIGSDPMFTAVLIGLGFRDLSSALPLMKDVAEKVSEISTWKAKLLAEQVISLAGE
EKFDEIEKLVLETKG
>Mature_575_residues
MEEKTTFKGISAYPGTVYGKVFRWKQTKRKREDRTDLSPEEIKEEVELLKKGLQKTEDDLADLVQKSKQNQELSEILESQ
IVFLNDPLFRARVFERIAQNNESAGLALETAVSSLYDEFQSIPDEFFRERADHLLDIGKRIESNLYPEKGSEPTKIPDDV
ILIAKEITPSEMIQLGKSKLRGIATDFGGKTGHTAIIARNYGIPTIVGLKNITSHVEDDDYILLDATKGVLNRSPGIEEI
KLAGIRSDIKKIIPVREISDGPKELKTKDGKTFTLRANIDSEEEVDGAFLQGADGIGLVRTEILFIRYVEFKPTEEEQFA
VYKRILLKMVGRPVTFRVWDIGADKMENGYEEENPFLGNRGIRYLLRHPHFFKEQLRALLRASEYGTMRIMLPMITTRSE
ILQTKVLLNECLDELKQTGLFITKKIPLGIMVETPACALNLPFLGNHVDFYSVGTNDLLQYLLAVERNNHLVGDLYNPWQ
VVFLLLLKNIVEVANSQKKPISICGEIGSDPMFTAVLIGLGFRDLSSALPLMKDVAEKVSEISTWKAKLLAEQVISLAGE
EKFDEIEKLVLETKG

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=557, Percent_Identity=35.1885098743267, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1788726, Length=527, Percent_Identity=31.8785578747628, Blast_Score=254, Evalue=8e-69,
Organism=Escherichia coli, GI1789193, Length=465, Percent_Identity=34.4086021505376, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI48994992, Length=492, Percent_Identity=31.5040650406504, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI226510935, Length=219, Percent_Identity=24.6575342465753, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64953; Mature: 64953

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKTTFKGISAYPGTVYGKVFRWKQTKRKREDRTDLSPEEIKEEVELLKKGLQKTEDDL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ADLVQKSKQNQELSEILESQIVFLNDPLFRARVFERIAQNNESAGLALETAVSSLYDEFQ
HHHHHHHHCCHHHHHHHHHCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
SIPDEFFRERADHLLDIGKRIESNLYPEKGSEPTKIPDDVILIAKEITPSEMIQLGKSKL
HCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
RGIATDFGGKTGHTAIIARNYGIPTIVGLKNITSHVEDDDYILLDATKGVLNRSPGIEEI
HHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHCCCCCHHHH
KLAGIRSDIKKIIPVREISDGPKELKTKDGKTFTLRANIDSEEEVDGAFLQGADGIGLVR
HHHHHHHHHHHHCCHHHCCCCHHHHHCCCCCEEEEEECCCCCHHHCCHHHCCCCCCHHHH
TEILFIRYVEFKPTEEEQFAVYKRILLKMVGRPVTFRVWDIGADKMENGYEEENPFLGNR
HHEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCCCCCCCCCCC
GIRYLLRHPHFFKEQLRALLRASEYGTMRIMLPMITTRSEILQTKVLLNECLDELKQTGL
HHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
FITKKIPLGIMVETPACALNLPFLGNHVDFYSVGTNDLLQYLLAVERNNHLVGDLYNPWQ
EEEEECCCEEEEECCCCEEECCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEECCCCHHH
VVFLLLLKNIVEVANSQKKPISICGEIGSDPMFTAVLIGLGFRDLSSALPLMKDVAEKVS
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
EISTWKAKLLAEQVISLAGEEKFDEIEKLVLETKG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEEKTTFKGISAYPGTVYGKVFRWKQTKRKREDRTDLSPEEIKEEVELLKKGLQKTEDDL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ADLVQKSKQNQELSEILESQIVFLNDPLFRARVFERIAQNNESAGLALETAVSSLYDEFQ
HHHHHHHHCCHHHHHHHHHCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
SIPDEFFRERADHLLDIGKRIESNLYPEKGSEPTKIPDDVILIAKEITPSEMIQLGKSKL
HCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
RGIATDFGGKTGHTAIIARNYGIPTIVGLKNITSHVEDDDYILLDATKGVLNRSPGIEEI
HHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHCCCCCHHHH
KLAGIRSDIKKIIPVREISDGPKELKTKDGKTFTLRANIDSEEEVDGAFLQGADGIGLVR
HHHHHHHHHHHHCCHHHCCCCHHHHHCCCCCEEEEEECCCCCHHHCCHHHCCCCCCHHHH
TEILFIRYVEFKPTEEEQFAVYKRILLKMVGRPVTFRVWDIGADKMENGYEEENPFLGNR
HHEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCCCCCCCCCCC
GIRYLLRHPHFFKEQLRALLRASEYGTMRIMLPMITTRSEILQTKVLLNECLDELKQTGL
HHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
FITKKIPLGIMVETPACALNLPFLGNHVDFYSVGTNDLLQYLLAVERNNHLVGDLYNPWQ
EEEEECCCEEEEECCCCEEECCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEECCCCHHH
VVFLLLLKNIVEVANSQKKPISICGEIGSDPMFTAVLIGLGFRDLSSALPLMKDVAEKVS
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
EISTWKAKLLAEQVISLAGEEKFDEIEKLVLETKG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA