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The map label for this gene is ddlB

Identifier: 183221247

GI number: 183221247

Start: 1953484

End: 1954533

Strand: Reverse

Name: ddlB

Synonym: LEPBI_I1863

Alternate gene names: 183221247

Gene position: 1954533-1953484 (Counterclockwise)

Preceding gene: 183221254

Following gene: 183221241

Centisome position: 54.3

GC content: 42.29

Gene sequence:

>1050_bases
ATGTCAAAAATCAAAATAGCTCTACTTTTCGGAGGCATCTCTGGAGAGCATATCATTTCTGTTCGTTCTTCTGCCTTTAT
TTTTGCGACCATTGATAGGGAAAAATATGACGTTTGTCCCGTCTATATCAATCCCAATGGAAAGTTTTGGATCCCGACAG
TGTCCGAACCCATCTACCCAGATCCGTCGGGCAAAACGGAAATTGAATTTTTACAAGAATTTAACAAAGCCAATGCAATT
GTTTCTCCGAGTGAACCTGCCGATATCAGTCAGATGGGATTTTTGTCCGCCTTCCTTGGATTACACGGGGGAGCCGGTGA
AGATGGAAGGATCCAAGGGTTTTTGGATACACTGGGAATTCCGCACACTGGTTCGGGTGTCCTTGCCTCAAGCCTCGCCA
TGGATAAATACCGGGCCAATATTTTATTTGAAGCCATGGGGATCCCTGTTGCACCTTTTTTAGAATTAGAAAAGGGGAAA
ACGGATCCAAGAAAAACTCTCCTCAACCTTTCCTTTTCGTATCCAGTATTCATTAAACCAACATTAGGCGGCTCAAGTGT
CAATACAGGAATGGCAAAAACTGCAGAAGAAGCCATGACTCTTGTGGATAAAATTTTTGTCACCGATGACCGAGTTCTTG
TTCAAAAACTTGTTTCCGGAACAGAAGTATCCATAGGTGTTTTAGAAAAACCGGAAGGGAAAAAACGAAATCCATTCCCT
TTGGTTCCTACAGAAATCAGACCCAAATCAGAATTTTTTGATTTTGAAGCAAAATACACCAAAGGTGCCAGTGAAGAGAT
CACACCTGCCCCTGTGGGGGATGAGGTGACAAAAACCTTACAGGAGTATACATTAAGGTGCCATGAAATTTTAGGTTGTA
AAGGTTATTCCCGGACAGACTTTATCATCTCCGATGGAGTTCCCTATGTACTAGAAACCAATACCTTACCTGGAATGACG
GGAACAAGTCTCATCCCTCAACAAGCGAAAGCCTTGGGGATCAATATGAAAGATGTATTTACGTGGTTACTTGAGATTTC
TCTTTCTTAG

Upstream 100 bases:

>100_bases
AGAAAGAAAAGTGAAAAAACAATTCCGAATTGAATTTTAAAGCGCCAGTTCACGTAATTTCATTATCGTTCACTTTCATG
AAAAGGAATCAAGTGATTTC

Downstream 100 bases:

>100_bases
GGAAGAGATACATTCCAATGAGAATGGCCACAAGGCTTGCGATGGTTCCGTAAAAATGACTGGTCATATCATCGCCATAA
ATCTCAAGTAAGAGCCAAGT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSKIKIALLFGGISGEHIISVRSSAFIFATIDREKYDVCPVYINPNGKFWIPTVSEPIYPDPSGKTEIEFLQEFNKANAI
VSPSEPADISQMGFLSAFLGLHGGAGEDGRIQGFLDTLGIPHTGSGVLASSLAMDKYRANILFEAMGIPVAPFLELEKGK
TDPRKTLLNLSFSYPVFIKPTLGGSSVNTGMAKTAEEAMTLVDKIFVTDDRVLVQKLVSGTEVSIGVLEKPEGKKRNPFP
LVPTEIRPKSEFFDFEAKYTKGASEEITPAPVGDEVTKTLQEYTLRCHEILGCKGYSRTDFIISDGVPYVLETNTLPGMT
GTSLIPQQAKALGINMKDVFTWLLEISLS

Sequences:

>Translated_349_residues
MSKIKIALLFGGISGEHIISVRSSAFIFATIDREKYDVCPVYINPNGKFWIPTVSEPIYPDPSGKTEIEFLQEFNKANAI
VSPSEPADISQMGFLSAFLGLHGGAGEDGRIQGFLDTLGIPHTGSGVLASSLAMDKYRANILFEAMGIPVAPFLELEKGK
TDPRKTLLNLSFSYPVFIKPTLGGSSVNTGMAKTAEEAMTLVDKIFVTDDRVLVQKLVSGTEVSIGVLEKPEGKKRNPFP
LVPTEIRPKSEFFDFEAKYTKGASEEITPAPVGDEVTKTLQEYTLRCHEILGCKGYSRTDFIISDGVPYVLETNTLPGMT
GTSLIPQQAKALGINMKDVFTWLLEISLS
>Mature_348_residues
SKIKIALLFGGISGEHIISVRSSAFIFATIDREKYDVCPVYINPNGKFWIPTVSEPIYPDPSGKTEIEFLQEFNKANAIV
SPSEPADISQMGFLSAFLGLHGGAGEDGRIQGFLDTLGIPHTGSGVLASSLAMDKYRANILFEAMGIPVAPFLELEKGKT
DPRKTLLNLSFSYPVFIKPTLGGSSVNTGMAKTAEEAMTLVDKIFVTDDRVLVQKLVSGTEVSIGVLEKPEGKKRNPFPL
VPTEIRPKSEFFDFEAKYTKGASEEITPAPVGDEVTKTLQEYTLRCHEILGCKGYSRTDFIISDGVPYVLETNTLPGMTG
TSLIPQQAKALGINMKDVFTWLLEISLS

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=352, Percent_Identity=35.5113636363636, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1786579, Length=374, Percent_Identity=29.144385026738, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_LEPBA (B0S9U5)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962893.1
- ProteinModelPortal:   B0S9U5
- SMR:   B0S9U5
- GeneID:   6388800
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1808
- HOGENOM:   HBG427092
- OMA:   RAFIILH
- ProtClustDB:   PRK14572
- BioCyc:   LBIF355278:LBF_1808-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 38026; Mature: 37895

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIKIALLFGGISGEHIISVRSSAFIFATIDREKYDVCPVYINPNGKFWIPTVSEPIYP
CCCEEEEEEEECCCCCEEEEEECCEEEEEEECCCCCEEEEEEECCCCCEEECCCCCCCCC
DPSGKTEIEFLQEFNKANAIVSPSEPADISQMGFLSAFLGLHGGAGEDGRIQGFLDTLGI
CCCCCHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEHHHCCC
PHTGSGVLASSLAMDKYRANILFEAMGIPVAPFLELEKGKTDPRKTLLNLSFSYPVFIKP
CCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCEEECCCCCCHHHHEEEEEECCCEEEEE
TLGGSSVNTGMAKTAEEAMTLVDKIFVTDDRVLVQKLVSGTEVSIGVLEKPEGKKRNPFP
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCC
LVPTEIRPKSEFFDFEAKYTKGASEEITPAPVGDEVTKTLQEYTLRCHEILGCKGYSRTD
CCCCCCCCCHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
FIISDGVPYVLETNTLPGMTGTSLIPQQAKALGINMKDVFTWLLEISLS
EEEECCCCEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHEECC
>Mature Secondary Structure 
SKIKIALLFGGISGEHIISVRSSAFIFATIDREKYDVCPVYINPNGKFWIPTVSEPIYP
CCEEEEEEEECCCCCEEEEEECCEEEEEEECCCCCEEEEEEECCCCCEEECCCCCCCCC
DPSGKTEIEFLQEFNKANAIVSPSEPADISQMGFLSAFLGLHGGAGEDGRIQGFLDTLGI
CCCCCHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEHHHCCC
PHTGSGVLASSLAMDKYRANILFEAMGIPVAPFLELEKGKTDPRKTLLNLSFSYPVFIKP
CCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCCEEECCCCCCHHHHEEEEEECCCEEEEE
TLGGSSVNTGMAKTAEEAMTLVDKIFVTDDRVLVQKLVSGTEVSIGVLEKPEGKKRNPFP
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCC
LVPTEIRPKSEFFDFEAKYTKGASEEITPAPVGDEVTKTLQEYTLRCHEILGCKGYSRTD
CCCCCCCCCHHHCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
FIISDGVPYVLETNTLPGMTGTSLIPQQAKALGINMKDVFTWLLEISLS
EEEECCCCEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA