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The map label for this gene is rfaF [H]

Identifier: 183221175

GI number: 183221175

Start: 1867400

End: 1868461

Strand: Reverse

Name: rfaF [H]

Synonym: LEPBI_I1789

Alternate gene names: 183221175

Gene position: 1868461-1867400 (Counterclockwise)

Preceding gene: 183221176

Following gene: 183221174

Centisome position: 51.91

GC content: 38.7

Gene sequence:

>1062_bases
ATGCCTGAAAAAATACTGATCATCCAAACCGCTTTTTTAGGAGATTTGATCCTATCGACCTCCTTTTTCCATGCTGTGAA
AATGGAACACAAGGAAAGCGAAATCCATGTCTTAGTCAATTTGGGTACGGAGTCGGTCCTAGACCAAAATCCTGACATAA
CTAAAGTTTGGTGTTTGGATAAAAAAAGGATCAAAAAGAATCCCTTTTTTTTCCTAAAATTTGTTAAAAAATTAAGATCA
GAAAGATTTGATAAGGTTTACTCTCCTCATTTTTCCTATCGATCGAGCCTCATTTCCTTTTTTACAAAGGCACCCATTCG
CATTGGTTATAAGGAATCGGGATTTTCTTTTTTGCACACGAAGGTAATCCAAAGACCCAAACAAGGTCCTCATGAAGTGG
AAAAATTATTTTCTCTCTTATTTGAACCTTATGATTTTCCAACGGGAAGGGAAAGGCAACCTTACTTGTATCCAGGTACC
GAGGAAGAATCGAGTTTTGCTCTCAAAAAATCAAAACTCATAAAAAATGACTCGGGTTATATCCTCATTGCCCCGTCATC
CTTGTGGGAAACAAAACGGTTGCCTGAAGAAAAATTTGTCAGTGTCATCACTCAAATCTTGCGAAAACGAAATGAAACTG
TGATCCTCATCGGAAGTAAGGCAGATATAGAGATTCAGAATCATATTTTCCGTTTGATGAAAACAGAGCCACTTGAATTA
AAAGAAAGGGAAAGGTTGTTATCACTTGTTGGCCAAACGAGTTTAAAAGAACTTATGGTTTGGATCCAAAATGCGACTGC
CATTCTTTCGAATGATTCGAGTCCCATTCACTTTGCATCTGCCTTTAATACACCTACAGTGATGATTTATGGCGCCACAA
TACCGGCGTTTGGTTATGGAAGTTTGTCTGACAAACACCGTATTATGGAAGTGAATGGATTAAACTGCAGACCATGTGGC
ATTCACGGAGGTAGGGTTTGCCCAGAAGGGCATTTTCGGTGTATGTTGGATCAAAATCCAGTACGTATTTTTGAAGCACT
CGAGGAAGTGATTAAGACATGA

Upstream 100 bases:

>100_bases
GCCGCTTCTTTTCTGAATGGCCCGAAAAGCGGTTGGCAGTAGCACAAAGTATAGGTGGACTAGCTACAAACAACTTTGTG
TCGATATTAGAAAGAGTCTG

Downstream 100 bases:

>100_bases
AACTACCGGTTATGAAATCGAATGAATTCGATGCAGGACTATTTCAAAAACGAATTCGGAAAGTCCAAAAAAAGCTAAAA
AAGGGCGAAATATTAATCTT

Product: putative ADP-heptose:LPS heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MPEKILIIQTAFLGDLILSTSFFHAVKMEHKESEIHVLVNLGTESVLDQNPDITKVWCLDKKRIKKNPFFFLKFVKKLRS
ERFDKVYSPHFSYRSSLISFFTKAPIRIGYKESGFSFLHTKVIQRPKQGPHEVEKLFSLLFEPYDFPTGRERQPYLYPGT
EEESSFALKKSKLIKNDSGYILIAPSSLWETKRLPEEKFVSVITQILRKRNETVILIGSKADIEIQNHIFRLMKTEPLEL
KERERLLSLVGQTSLKELMVWIQNATAILSNDSSPIHFASAFNTPTVMIYGATIPAFGYGSLSDKHRIMEVNGLNCRPCG
IHGGRVCPEGHFRCMLDQNPVRIFEALEEVIKT

Sequences:

>Translated_353_residues
MPEKILIIQTAFLGDLILSTSFFHAVKMEHKESEIHVLVNLGTESVLDQNPDITKVWCLDKKRIKKNPFFFLKFVKKLRS
ERFDKVYSPHFSYRSSLISFFTKAPIRIGYKESGFSFLHTKVIQRPKQGPHEVEKLFSLLFEPYDFPTGRERQPYLYPGT
EEESSFALKKSKLIKNDSGYILIAPSSLWETKRLPEEKFVSVITQILRKRNETVILIGSKADIEIQNHIFRLMKTEPLEL
KERERLLSLVGQTSLKELMVWIQNATAILSNDSSPIHFASAFNTPTVMIYGATIPAFGYGSLSDKHRIMEVNGLNCRPCG
IHGGRVCPEGHFRCMLDQNPVRIFEALEEVIKT
>Mature_352_residues
PEKILIIQTAFLGDLILSTSFFHAVKMEHKESEIHVLVNLGTESVLDQNPDITKVWCLDKKRIKKNPFFFLKFVKKLRSE
RFDKVYSPHFSYRSSLISFFTKAPIRIGYKESGFSFLHTKVIQRPKQGPHEVEKLFSLLFEPYDFPTGRERQPYLYPGTE
EESSFALKKSKLIKNDSGYILIAPSSLWETKRLPEEKFVSVITQILRKRNETVILIGSKADIEIQNHIFRLMKTEPLELK
ERERLLSLVGQTSLKELMVWIQNATAILSNDSSPIHFASAFNTPTVMIYGATIPAFGYGSLSDKHRIMEVNGLNCRPCGI
HGGRVCPEGHFRCMLDQNPVRIFEALEEVIKT

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=355, Percent_Identity=25.0704225352113, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1790062, Length=307, Percent_Identity=26.0586319218241, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 40585; Mature: 40454

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEKILIIQTAFLGDLILSTSFFHAVKMEHKESEIHVLVNLGTESVLDQNPDITKVWCLD
CCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHCCCCCEEEEEECC
KKRIKKNPFFFLKFVKKLRSERFDKVYSPHFSYRSSLISFFTKAPIRIGYKESGFSFLHT
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHH
KVIQRPKQGPHEVEKLFSLLFEPYDFPTGRERQPYLYPGTEEESSFALKKSKLIKNDSGY
HHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCE
ILIAPSSLWETKRLPEEKFVSVITQILRKRNETVILIGSKADIEIQNHIFRLMKTEPLEL
EEEECCHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCH
KERERLLSLVGQTSLKELMVWIQNATAILSNDSSPIHFASAFNTPTVMIYGATIPAFGYG
HHHHHHHHHHCHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCCCEEEEEECCCCCCCCC
SLSDKHRIMEVNGLNCRPCGIHGGRVCPEGHFRCMLDQNPVRIFEALEEVIKT
CCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PEKILIIQTAFLGDLILSTSFFHAVKMEHKESEIHVLVNLGTESVLDQNPDITKVWCLD
CCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHCCCCCEEEEEECC
KKRIKKNPFFFLKFVKKLRSERFDKVYSPHFSYRSSLISFFTKAPIRIGYKESGFSFLHT
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHH
KVIQRPKQGPHEVEKLFSLLFEPYDFPTGRERQPYLYPGTEEESSFALKKSKLIKNDSGY
HHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCE
ILIAPSSLWETKRLPEEKFVSVITQILRKRNETVILIGSKADIEIQNHIFRLMKTEPLEL
EEEECCHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCH
KERERLLSLVGQTSLKELMVWIQNATAILSNDSSPIHFASAFNTPTVMIYGATIPAFGYG
HHHHHHHHHHCHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCCCEEEEEECCCCCCCCC
SLSDKHRIMEVNGLNCRPCGIHGGRVCPEGHFRCMLDQNPVRIFEALEEVIKT
CCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]