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The map label for this gene is mnmC [H]

Identifier: 183220345

GI number: 183220345

Start: 973221

End: 974417

Strand: Reverse

Name: mnmC [H]

Synonym: LEPBI_I0941

Alternate gene names: 183220345

Gene position: 974417-973221 (Counterclockwise)

Preceding gene: 183220346

Following gene: 183220342

Centisome position: 27.07

GC content: 39.01

Gene sequence:

>1197_bases
ATGAGAACCCTTGAGGAAAAAACTGCCATTGTCGTAGGGAGCGGGATTGCAGGAGCAAGCATTTGTTATGCGCTTTCCAA
ACAAAATATCAATACCATTTTATTGGAATCGGAATCAGCACCAGCAAAACATGCTAGTGGAAATCCAATTGGTGTCGTGT
ATCCCTTTCTCACCAAACACAAAACTGCGGAATCAGAATTTTCGCTAATAGCATACACTCACTTTTTGGAAGTTTGGGAA
AGATTGGGATTGGAAAATTCAGTTCCTCATGCCAATGGAATCCATTTTTTATTGGAAACAGAATCCAACGTAGACCGTTA
TTGGCACTCGCTCCTTTCCCATCAAATTCCCGAATCAATCGCCAAACAAAGTTTTGAGCCTAATTCCCAAAAAAAGGCTT
TGTACTTCCCAAAAGGGAAAGCTCTCTCTCCCGCCATCCTTACAAAAGAACTGATTCGATTGGCACACCCTACAATGAAA
TTGCAATCCACATTAGTCTCCTGGAGAGAGAAAGAATCGGATGGTAAATTGATTTGCGAAACAAATGAAGAAAAGTTTGA
AGTCGATTTTTTATTTTTGGCGCAAGGGTATCAATTTACGAAGGATCCGTTCTCAAATTGGATTCCGATGAAACAAGTTC
GGGGGCAAATTGTACAAATTCCTTCAGCTTTTTTTCAAAACCAAACCTCTATTTTGTATGGAGATTACTTAACAGCCGAA
ATCAATGGGGAACGCGTATTAGGTGCAAGTTTCGATGAATTCCATCTAGAAATAGAACCTAGAATCAATGAAACCATAAC
CATGTGGGAAACTCTGCAATCAAAACTGCCAATCTTACATAACGATTGGAAAACTGTGGATGTGAGTCAGTTTGGAACTC
GTGTGAGTTATCGGACACAGTCCCAAGATCGTCACCCAGTAGTGGGAAAACTTCCCAATCTTTCAACTTTAGATACTACT
GTGAAATACCAAAATATGTTCCGAAAAGATCACAAACCATTCCAAATACCATATTATGAATCTGTCGGAATTTTAAATGG
TTTAGGATCCAGAGGTCTCACACACTCTTTACTTGCAGCTGAAATTTTAGTGAAATCAATTTTAAATCAAACGATGGACA
TTCCAAACAATCTCATCAATTCATTGAAACCAGATCGTTTTTTGCTTCGTATGTGGAAACGAGACCAGCTAACATAG

Upstream 100 bases:

>100_bases
CATTCGAAGGAATTTAGAATCATTGGGATTTTTTGTACAAAAACAAAAAGGGTTTGGGAAGAAGAGAGAAATGTTAACAG
GAGTTCTACTGTAATCTGAA

Downstream 100 bases:

>100_bases
ATTCGAAACTTTCTTTCTCATTCACAACCACTTCAATTTTGATCACAAAGATTTGAATGTTTCTTTTTTTTAATTCTTCC
AAAAATCCTTTTTTTGTACG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MRTLEEKTAIVVGSGIAGASICYALSKQNINTILLESESAPAKHASGNPIGVVYPFLTKHKTAESEFSLIAYTHFLEVWE
RLGLENSVPHANGIHFLLETESNVDRYWHSLLSHQIPESIAKQSFEPNSQKKALYFPKGKALSPAILTKELIRLAHPTMK
LQSTLVSWREKESDGKLICETNEEKFEVDFLFLAQGYQFTKDPFSNWIPMKQVRGQIVQIPSAFFQNQTSILYGDYLTAE
INGERVLGASFDEFHLEIEPRINETITMWETLQSKLPILHNDWKTVDVSQFGTRVSYRTQSQDRHPVVGKLPNLSTLDTT
VKYQNMFRKDHKPFQIPYYESVGILNGLGSRGLTHSLLAAEILVKSILNQTMDIPNNLINSLKPDRFLLRMWKRDQLT

Sequences:

>Translated_398_residues
MRTLEEKTAIVVGSGIAGASICYALSKQNINTILLESESAPAKHASGNPIGVVYPFLTKHKTAESEFSLIAYTHFLEVWE
RLGLENSVPHANGIHFLLETESNVDRYWHSLLSHQIPESIAKQSFEPNSQKKALYFPKGKALSPAILTKELIRLAHPTMK
LQSTLVSWREKESDGKLICETNEEKFEVDFLFLAQGYQFTKDPFSNWIPMKQVRGQIVQIPSAFFQNQTSILYGDYLTAE
INGERVLGASFDEFHLEIEPRINETITMWETLQSKLPILHNDWKTVDVSQFGTRVSYRTQSQDRHPVVGKLPNLSTLDTT
VKYQNMFRKDHKPFQIPYYESVGILNGLGSRGLTHSLLAAEILVKSILNQTMDIPNNLINSLKPDRFLLRMWKRDQLT
>Mature_398_residues
MRTLEEKTAIVVGSGIAGASICYALSKQNINTILLESESAPAKHASGNPIGVVYPFLTKHKTAESEFSLIAYTHFLEVWE
RLGLENSVPHANGIHFLLETESNVDRYWHSLLSHQIPESIAKQSFEPNSQKKALYFPKGKALSPAILTKELIRLAHPTMK
LQSTLVSWREKESDGKLICETNEEKFEVDFLFLAQGYQFTKDPFSNWIPMKQVRGQIVQIPSAFFQNQTSILYGDYLTAE
INGERVLGASFDEFHLEIEPRINETITMWETLQSKLPILHNDWKTVDVSQFGTRVSYRTQSQDRHPVVGKLPNLSTLDTT
VKYQNMFRKDHKPFQIPYYESVGILNGLGSRGLTHSLLAAEILVKSILNQTMDIPNNLINSLKPDRFLLRMWKRDQLT

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=402, Percent_Identity=25.3731343283582, Blast_Score=109, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 45337; Mature: 45337

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLEEKTAIVVGSGIAGASICYALSKQNINTILLESESAPAKHASGNPIGVVYPFLTKH
CCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEECHHHCC
KTAESEFSLIAYTHFLEVWERLGLENSVPHANGIHFLLETESNVDRYWHSLLSHQIPESI
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
AKQSFEPNSQKKALYFPKGKALSPAILTKELIRLAHPTMKLQSTLVSWREKESDGKLICE
HHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEE
TNEEKFEVDFLFLAQGYQFTKDPFSNWIPMKQVRGQIVQIPSAFFQNQTSILYGDYLTAE
CCCCEEEEEEEEEECCCCCCCCCHHCCCCHHHHCCHHEECCHHHHCCCCEEEECCEEEEE
INGERVLGASFDEFHLEIEPRINETITMWETLQSKLPILHNDWKTVDVSQFGTRVSYRTQ
ECCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEECCCCEEEHHHHCCEEEEECC
SQDRHPVVGKLPNLSTLDTTVKYQNMFRKDHKPFQIPYYESVGILNGLGSRGLTHSLLAA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCHHCCHHHHCCCCCCHHHHHHHH
EILVKSILNQTMDIPNNLINSLKPDRFLLRMWKRDQLT
HHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MRTLEEKTAIVVGSGIAGASICYALSKQNINTILLESESAPAKHASGNPIGVVYPFLTKH
CCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEECHHHCC
KTAESEFSLIAYTHFLEVWERLGLENSVPHANGIHFLLETESNVDRYWHSLLSHQIPESI
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
AKQSFEPNSQKKALYFPKGKALSPAILTKELIRLAHPTMKLQSTLVSWREKESDGKLICE
HHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEE
TNEEKFEVDFLFLAQGYQFTKDPFSNWIPMKQVRGQIVQIPSAFFQNQTSILYGDYLTAE
CCCCEEEEEEEEEECCCCCCCCCHHCCCCHHHHCCHHEECCHHHHCCCCEEEECCEEEEE
INGERVLGASFDEFHLEIEPRINETITMWETLQSKLPILHNDWKTVDVSQFGTRVSYRTQ
ECCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEECCCCEEEHHHHCCEEEEECC
SQDRHPVVGKLPNLSTLDTTVKYQNMFRKDHKPFQIPYYESVGILNGLGSRGLTHSLLAA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCHHCCHHHHCCCCCCHHHHHHHH
EILVKSILNQTMDIPNNLINSLKPDRFLLRMWKRDQLT
HHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA