Click here to switch to the map view.

The map label for this gene is mnmC [H]

Identifier: 183220346

GI number: 183220346

Start: 974424

End: 975293

Strand: Reverse

Name: mnmC [H]

Synonym: LEPBI_I0942

Alternate gene names: 183220346

Gene position: 975293-974424 (Counterclockwise)

Preceding gene: 183220356

Following gene: 183220345

Centisome position: 27.09

GC content: 42.07

Gene sequence:

>870_bases
ATGAGACATAATAAATTTGCCAAGAAGGGTGTCAGGATTTCCTTTTTCCCATTGGTTAAAAGAGAATCCGTTTCCCTCCC
CTCTCCCTTTCACCATCTTGGTATCTGGGAAGTGAGATCACGATCAATGGATCCAATGCGAACCGAAGCCAAGATAGTAT
TGCAGGAAGGAGTCCCTGTCTCTTTGCACTATGATGATGTGTACTTTTCGAAGGAAGGCGGTTGGGAAGAATCAAAGTAT
GTGTTTCTGGAAGGAAACCGCATCCCAGAAGCCTTCTCAAAGAAGGAAATCAGCCAAATCCAGATTGGTGAACTTGGATT
TGGGACGGGACTGAATTTCTTTGTGACCTTAGACCATTGGCAAAGGATCAATGACCCACCTTCGGTCCATTTCGTAAGCT
TAGAAGGGTTCCCTTTACCGAGTGAAATATTACACTCCCTGAATCTTTCCTTTCCGGGGAAACCTTTATGGACGGAAGCT
TTGCAAGAATCCTATACCGAACAAAGGAAACGTTGGAACTTGGATGCAAACGATATGATTTGGAAGGTCCAAATCCCCCA
CCAGAAATCCCCAAACCCCCAAACGACATTTACCCTAACTTTGTATTTAGGAGATGTATTGGAATGTTTGGAACTTTTTC
CCAAGATCGACTTTTGGTATTTGGATGGATTTTCTCCGAATAAAAACCCAAATATGTGGTCACCTGAAACACTTTCACAA
ATCCGAATCCATTCTAAGAAAGGCACTCGGTTTTCTACCTTTACAGCAGCGGGATTCATTCGAAGGAATTTAGAATCATT
GGGATTTTTTGTACAAAAACAAAAAGGGTTTGGGAAGAAGAGAGAAATGTTAACAGGAGTTCTACTGTAA

Upstream 100 bases:

>100_bases
GTCTTGTAAGTCTTTGCCTCGGATTTTCACAAAATCCATAGAATCTCCCCCAAACCTGGGAAAAATATGAAGGGTACCCT
CTATTTGGAAAGCCTTTTTT

Downstream 100 bases:

>100_bases
TCTGAAATGAGAACCCTTGAGGAAAAAACTGCCATTGTCGTAGGGAGCGGGATTGCAGGAGCAAGCATTTGTTATGCGCT
TTCCAAACAAAATATCAATA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MRHNKFAKKGVRISFFPLVKRESVSLPSPFHHLGIWEVRSRSMDPMRTEAKIVLQEGVPVSLHYDDVYFSKEGGWEESKY
VFLEGNRIPEAFSKKEISQIQIGELGFGTGLNFFVTLDHWQRINDPPSVHFVSLEGFPLPSEILHSLNLSFPGKPLWTEA
LQESYTEQRKRWNLDANDMIWKVQIPHQKSPNPQTTFTLTLYLGDVLECLELFPKIDFWYLDGFSPNKNPNMWSPETLSQ
IRIHSKKGTRFSTFTAAGFIRRNLESLGFFVQKQKGFGKKREMLTGVLL

Sequences:

>Translated_289_residues
MRHNKFAKKGVRISFFPLVKRESVSLPSPFHHLGIWEVRSRSMDPMRTEAKIVLQEGVPVSLHYDDVYFSKEGGWEESKY
VFLEGNRIPEAFSKKEISQIQIGELGFGTGLNFFVTLDHWQRINDPPSVHFVSLEGFPLPSEILHSLNLSFPGKPLWTEA
LQESYTEQRKRWNLDANDMIWKVQIPHQKSPNPQTTFTLTLYLGDVLECLELFPKIDFWYLDGFSPNKNPNMWSPETLSQ
IRIHSKKGTRFSTFTAAGFIRRNLESLGFFVQKQKGFGKKREMLTGVLL
>Mature_289_residues
MRHNKFAKKGVRISFFPLVKRESVSLPSPFHHLGIWEVRSRSMDPMRTEAKIVLQEGVPVSLHYDDVYFSKEGGWEESKY
VFLEGNRIPEAFSKKEISQIQIGELGFGTGLNFFVTLDHWQRINDPPSVHFVSLEGFPLPSEILHSLNLSFPGKPLWTEA
LQESYTEQRKRWNLDANDMIWKVQIPHQKSPNPQTTFTLTLYLGDVLECLELFPKIDFWYLDGFSPNKNPNMWSPETLSQ
IRIHSKKGTRFSTFTAAGFIRRNLESLGFFVQKQKGFGKKREMLTGVLL

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=248, Percent_Identity=38.3064516129032, Blast_Score=153, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 33468; Mature: 33468

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHNKFAKKGVRISFFPLVKRESVSLPSPFHHLGIWEVRSRSMDPMRTEAKIVLQEGVPV
CCCCHHHHCCCEEEEEEEEECCCCCCCCCHHHCCCHHHHCCCCCCCCCCEEEEEECCCEE
SLHYDDVYFSKEGGWEESKYVFLEGNRIPEAFSKKEISQIQIGELGFGTGLNFFVTLDHW
EEEECCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHCEEEECCCCCCCCCEEEEEEEHH
QRINDPPSVHFVSLEGFPLPSEILHSLNLSFPGKPLWTEALQESYTEQRKRWNLDANDMI
HCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHEE
WKVQIPHQKSPNPQTTFTLTLYLGDVLECLELFPKIDFWYLDGFSPNKNPNMWSPETLSQ
EEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHH
IRIHSKKGTRFSTFTAAGFIRRNLESLGFFVQKQKGFGKKREMLTGVLL
HEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MRHNKFAKKGVRISFFPLVKRESVSLPSPFHHLGIWEVRSRSMDPMRTEAKIVLQEGVPV
CCCCHHHHCCCEEEEEEEEECCCCCCCCCHHHCCCHHHHCCCCCCCCCCEEEEEECCCEE
SLHYDDVYFSKEGGWEESKYVFLEGNRIPEAFSKKEISQIQIGELGFGTGLNFFVTLDHW
EEEECCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHCEEEECCCCCCCCCEEEEEEEHH
QRINDPPSVHFVSLEGFPLPSEILHSLNLSFPGKPLWTEALQESYTEQRKRWNLDANDMI
HCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHEE
WKVQIPHQKSPNPQTTFTLTLYLGDVLECLELFPKIDFWYLDGFSPNKNPNMWSPETLSQ
EEEECCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHH
IRIHSKKGTRFSTFTAAGFIRRNLESLGFFVQKQKGFGKKREMLTGVLL
HEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA