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The map label for this gene is rsbU [H]

Identifier: 183220125

GI number: 183220125

Start: 722166

End: 723869

Strand: Reverse

Name: rsbU [H]

Synonym: LEPBI_I0713

Alternate gene names: 183220125

Gene position: 723869-722166 (Counterclockwise)

Preceding gene: 183220126

Following gene: 183220120

Centisome position: 20.11

GC content: 41.14

Gene sequence:

>1704_bases
TTGGACCCCCATCATCCTCATTGGGTATTTCATCTCAGCACAAATTGGTTCAATATTGCATTTCTCAATAGCCAAGTTTC
ACCTGTTTGGCCACCGGAAGGTGTAGGCCTTTCTTCTCTTTTACTCCTTGGACCTGTGGCTTTACCGGGAATTTACCTCG
GCGCCACCCTTGCCAATTTCTACAATAACCCACATTTCCAAACAGCGTTTATTATAGGAATTGGAAACACTCTCAGTAGT
TACGTCAATTACCGCATCATCAAACGAGTGACAGAAAAAACGGATCCTTTGTATTCCACAAAGGATTTAATTTATTTCCT
AAGCATTGGAACCTTTCCTGGATCTTTGATTAGCGCCGTGATGGGAGTGACGAGTTTATGGTATTGGGATTTTTTATCAT
CTGAATTGTATTTTAATGTTTTCTTTACTTGGTTTTCAGGAGAGATGCTTGGATTTCTCATCGTTGCCCCACTCCTGTAT
GTTTGGTTTTATCCCAAATCCAAATTAAACTTAGAACTTTCAAAACAATTGGAACTTTTTTTATGGATCGTCATCGTATA
TATTTCTGGATCCATTGCGTTCAGTGATGAATGGCCCCTCCTCTTTGTTCCCATTCCCTTCGTGATTGTCACAAGCATTC
GCTTCCGTCAATTTGGGGCCACCTTGTCAACGGTAGTTTTATCATTCATTGCAGTCACACTCACCATTGAAGGGAAAGGT
GTCTTTGCAAGACGTGATGCGAGTGGACTTTCGATCAATGATTCACTTATCTTTCTGGACGCGTTCTTATTTTGTATCAG
TGGGATAGCTTACTTCCTTGTGACGGCCACGCGCGAAAGAGAACGAGCCCAAGAAACGTCTCTAAAATCATTACAAGTGT
TAAACGAACTGAAAGAACAAGCCAATGAAGAACTAGAACAAAAAGTTTTAGAACGAACTGCTGTCATAGAGGAACAAAGG
ATCGAAATCGAAAAACAATTGGATATGGCAAAAAGAATCCAAGAATCCCTTTTCCCTCAAAAAGAAATTATACCTAATGG
TGTTGAGATTTTATTCAAAAACATTCCCATGATGAAAGTGGGAGGTGACTTGTATGACATTGTATGGAGACACGACAAGC
AAGAGTTAGGTGTATTTATTTGCGATGTGTCTGGGCATGGAATCCCTGCAGCCCTTTTAAGTGCCCTTGTCAAAACTTCA
CTTGAAAAATGGAAAGAAGACCCAAGTGACTTGAAGGAAAGTTTGGAATCCATCCGTAATCAAATCATACCGAATTTAAG
AGAACATTTTGTGACGGCAAGCTTTGTCCATCTACATACAGAATCAGGAGCACTTACATTTGCTCGTGCAGGCCATTTCC
CTTTATTCATCATTCGAAAATCAGGTGCCCTTGTTAGCTTAAAACCAATGGGTAGGATCATCACTCCCATCTTTGATATC
CTTGCGGAAGAAGAAAAATTCCAACTGGAAAAAGGTGATTTGATTGTGATGCTCACCGATGGCCTCACGGAAGCAAGAGA
CCCGTCTTCCTTACAAATGTTTGGTGAAGATAAACTACTCCACTTGGTTCGAGAACTACGAAACCAACCACTCGTCCAGA
TCCAAGACGAAGTTTTCCAATCGGTGATCCACTTATCTGGAGGAATCCAAGCCATCCAAGATGACTTAACTCTTGGGCTC
ATTCGTTATACGGGTGTCAGTTGA

Upstream 100 bases:

>100_bases
GAAGAAATCCAAACGATTGAGACGGAAATTCTTTACAAAATGGAGGAATGGGAAATGTTTCAAACCGAATGAAAACGGTC
CAGTATACAAGAATTCTCCT

Downstream 100 bases:

>100_bases
TACTTTCGATTCAAACAAAAGAATCTTTACTCATGAAAAAATGATTCTTTTGGGAATGTTCAATTTATCCTTGGTTTCAG
GTTTGAGTTAAAAATTCTTC

Product: putative phosphoserine phosphatase

Products: NA

Alternate protein names: Sigma factor sigB regulation protein rsbU [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MDPHHPHWVFHLSTNWFNIAFLNSQVSPVWPPEGVGLSSLLLLGPVALPGIYLGATLANFYNNPHFQTAFIIGIGNTLSS
YVNYRIIKRVTEKTDPLYSTKDLIYFLSIGTFPGSLISAVMGVTSLWYWDFLSSELYFNVFFTWFSGEMLGFLIVAPLLY
VWFYPKSKLNLELSKQLELFLWIVIVYISGSIAFSDEWPLLFVPIPFVIVTSIRFRQFGATLSTVVLSFIAVTLTIEGKG
VFARRDASGLSINDSLIFLDAFLFCISGIAYFLVTATRERERAQETSLKSLQVLNELKEQANEELEQKVLERTAVIEEQR
IEIEKQLDMAKRIQESLFPQKEIIPNGVEILFKNIPMMKVGGDLYDIVWRHDKQELGVFICDVSGHGIPAALLSALVKTS
LEKWKEDPSDLKESLESIRNQIIPNLREHFVTASFVHLHTESGALTFARAGHFPLFIIRKSGALVSLKPMGRIITPIFDI
LAEEEKFQLEKGDLIVMLTDGLTEARDPSSLQMFGEDKLLHLVRELRNQPLVQIQDEVFQSVIHLSGGIQAIQDDLTLGL
IRYTGVS

Sequences:

>Translated_567_residues
MDPHHPHWVFHLSTNWFNIAFLNSQVSPVWPPEGVGLSSLLLLGPVALPGIYLGATLANFYNNPHFQTAFIIGIGNTLSS
YVNYRIIKRVTEKTDPLYSTKDLIYFLSIGTFPGSLISAVMGVTSLWYWDFLSSELYFNVFFTWFSGEMLGFLIVAPLLY
VWFYPKSKLNLELSKQLELFLWIVIVYISGSIAFSDEWPLLFVPIPFVIVTSIRFRQFGATLSTVVLSFIAVTLTIEGKG
VFARRDASGLSINDSLIFLDAFLFCISGIAYFLVTATRERERAQETSLKSLQVLNELKEQANEELEQKVLERTAVIEEQR
IEIEKQLDMAKRIQESLFPQKEIIPNGVEILFKNIPMMKVGGDLYDIVWRHDKQELGVFICDVSGHGIPAALLSALVKTS
LEKWKEDPSDLKESLESIRNQIIPNLREHFVTASFVHLHTESGALTFARAGHFPLFIIRKSGALVSLKPMGRIITPIFDI
LAEEEKFQLEKGDLIVMLTDGLTEARDPSSLQMFGEDKLLHLVRELRNQPLVQIQDEVFQSVIHLSGGIQAIQDDLTLGL
IRYTGVS
>Mature_567_residues
MDPHHPHWVFHLSTNWFNIAFLNSQVSPVWPPEGVGLSSLLLLGPVALPGIYLGATLANFYNNPHFQTAFIIGIGNTLSS
YVNYRIIKRVTEKTDPLYSTKDLIYFLSIGTFPGSLISAVMGVTSLWYWDFLSSELYFNVFFTWFSGEMLGFLIVAPLLY
VWFYPKSKLNLELSKQLELFLWIVIVYISGSIAFSDEWPLLFVPIPFVIVTSIRFRQFGATLSTVVLSFIAVTLTIEGKG
VFARRDASGLSINDSLIFLDAFLFCISGIAYFLVTATRERERAQETSLKSLQVLNELKEQANEELEQKVLERTAVIEEQR
IEIEKQLDMAKRIQESLFPQKEIIPNGVEILFKNIPMMKVGGDLYDIVWRHDKQELGVFICDVSGHGIPAALLSALVKTS
LEKWKEDPSDLKESLESIRNQIIPNLREHFVTASFVHLHTESGALTFARAGHFPLFIIRKSGALVSLKPMGRIITPIFDI
LAEEEKFQLEKGDLIVMLTDGLTEARDPSSLQMFGEDKLLHLVRELRNQPLVQIQDEVFQSVIHLSGGIQAIQDDLTLGL
IRYTGVS

Specific function: Positive regulator of sigma-B activity. Dephosphorylates rsbV in response to environmental stress conveyed from the rsbXST module [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017944
- InterPro:   IPR001932
- InterPro:   IPR014787
- InterPro:   IPR010822
- ProDom:   PD685490 [H]

Pfam domain/function: PF08673 RsbU_N; PF07228 SpoIIE [H]

EC number: =3.1.3.3 [H]

Molecular weight: Translated: 64084; Mature: 64084

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPHHPHWVFHLSTNWFNIAFLNSQVSPVWPPEGVGLSSLLLLGPVALPGIYLGATLANF
CCCCCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHH
YNNPHFQTAFIIGIGNTLSSYVNYRIIKRVTEKTDPLYSTKDLIYFLSIGTFPGSLISAV
CCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHH
MGVTSLWYWDFLSSELYFNVFFTWFSGEMLGFLIVAPLLYVWFYPKSKLNLELSKQLELF
HHHHHHHHHHHHCCHHEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHH
LWIVIVYISGSIAFSDEWPLLFVPIPFVIVTSIRFRQFGATLSTVVLSFIAVTLTIEGKG
HHHHHHHHCCCEEECCCCCEEEECHHHHHHHHHHHHHHCHHHHHHHHHHHHEEEEECCCE
VFARRDASGLSINDSLIFLDAFLFCISGIAYFLVTATRERERAQETSLKSLQVLNELKEQ
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANEELEQKVLERTAVIEEQRIEIEKQLDMAKRIQESLFPQKEIIPNGVEILFKNIPMMKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHCCCCEEE
GGDLYDIVWRHDKQELGVFICDVSGHGIPAALLSALVKTSLEKWKEDPSDLKESLESIRN
CCCHHHHHHCCCCHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
QIIPNLREHFVTASFVHLHTESGALTFARAGHFPLFIIRKSGALVSLKPMGRIITPIFDI
HHCHHHHHHHHHEEEEEEEECCCCEEEEECCCCEEEEEECCCCEEEECCHHHHHHHHHHH
LAEEEKFQLEKGDLIVMLTDGLTEARDPSSLQMFGEDKLLHLVRELRNQPLVQIQDEVFQ
HCCHHHHEECCCCEEEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCCEEHHHHHHH
SVIHLSGGIQAIQDDLTLGLIRYTGVS
HHHHHCCCHHHHHHHHHEEEHEECCCC
>Mature Secondary Structure
MDPHHPHWVFHLSTNWFNIAFLNSQVSPVWPPEGVGLSSLLLLGPVALPGIYLGATLANF
CCCCCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHH
YNNPHFQTAFIIGIGNTLSSYVNYRIIKRVTEKTDPLYSTKDLIYFLSIGTFPGSLISAV
CCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHH
MGVTSLWYWDFLSSELYFNVFFTWFSGEMLGFLIVAPLLYVWFYPKSKLNLELSKQLELF
HHHHHHHHHHHHCCHHEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHH
LWIVIVYISGSIAFSDEWPLLFVPIPFVIVTSIRFRQFGATLSTVVLSFIAVTLTIEGKG
HHHHHHHHCCCEEECCCCCEEEECHHHHHHHHHHHHHHCHHHHHHHHHHHHEEEEECCCE
VFARRDASGLSINDSLIFLDAFLFCISGIAYFLVTATRERERAQETSLKSLQVLNELKEQ
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANEELEQKVLERTAVIEEQRIEIEKQLDMAKRIQESLFPQKEIIPNGVEILFKNIPMMKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHCCCCEEE
GGDLYDIVWRHDKQELGVFICDVSGHGIPAALLSALVKTSLEKWKEDPSDLKESLESIRN
CCCHHHHHHCCCCHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
QIIPNLREHFVTASFVHLHTESGALTFARAGHFPLFIIRKSGALVSLKPMGRIITPIFDI
HHCHHHHHHHHHEEEEEEEECCCCEEEEECCCCEEEEEECCCCEEEECCHHHHHHHHHHH
LAEEEKFQLEKGDLIVMLTDGLTEARDPSSLQMFGEDKLLHLVRELRNQPLVQIQDEVFQ
HCCHHHHEECCCCEEEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCCEEHHHHHHH
SVIHLSGGIQAIQDDLTLGLIRYTGVS
HHHHHCCCHHHHHHHHHEEEHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8002610; 8002609; 9384377; 8824586; 9786195; 12499568 [H]