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The map label for this gene is thlA [H]

Identifier: 183220119

GI number: 183220119

Start: 717252

End: 718571

Strand: Reverse

Name: thlA [H]

Synonym: LEPBI_I0707

Alternate gene names: 183220119

Gene position: 718571-717252 (Counterclockwise)

Preceding gene: 183220120

Following gene: 183220117

Centisome position: 19.96

GC content: 43.86

Gene sequence:

>1320_bases
ATGAAAGTAACACAAAAGATTGTACTCGCAGCACCTGCACGAACTCCTTTTGCACAAATTGGCAAGGCGCTCGCTCAGTA
TCCAGGCCACCACTTAGGGAAACTTGTTGGTGATGAAGTTATGAAACGCAGTGGTTTGAAACCAACTGACATTGACGGTG
TGATTGTGGGAGAGGGTTTTGCCAATGCACCAAACTCTGCTCGTGTGATTGCAAACCTCATGAACCTGCCTTTAGAAATT
CCTTGTTTAACAGTGGCAAACAACTGTGTATCAGGTCTCGAAGCAGTGGCAGAAGCATCTCGCCGAATTATGTTAGGTGA
AGGGGAAGTTTTCCTCGTGATTGGTGAAGAATCTCAAACTTCTATGCCTTTCGTTGTGAAAAATGCTCGCCTCAACAAAA
AAACAAACAGCTTAGATTCACTTGTAAAACTCCTTCCAAACGACCTTCCAGAAGGTGTGGAACTTCGTGATACATTGGAA
GATGGTCTTGGTGATGGAGAAACTTCTTATGGGATGCAAGTCACAGCAGAAATCCTCGCACAAAACTACGCACTTCCACG
TGAAACGCAAGACAAAGTGGCTTACGAATCTTTCAAAAGAGCTTTCGAAGCAACTCAAGAAGGTCGTTACAAACCGTATA
TTATGGAAGTAAAAGACGATGAAGGGAACATGTTACAAGCTGACGAAGCAGTTCTCCTTCGTGAAGGTCTTGTGAAAAAC
CCAACTCGTATGGGTCGCGCAATGCTTATGTTTGATAACCCAGCAATGAAATTTGATGCTTGGAAAGAAAAGTACGGAAA
AGATTTAAAAAAATCACATGGCCCTACGGTTTCCATCTTCAATGCAAGCCCACGTTCTGATGGAGCGGCTGGAATCATTG
TCGCTTCTGAAGCTGCTGCAAAAAGATTAGGTCTGAAAGCAGAAGCAGTGGTCACAGGTTTCAAAATGAAAGGTGTGGAT
CCTAACCTTATGGGACTTGGACAAGCAGAAGCAACTCTTGGACTACTCGAAGAAGTTGGCGAAAAAGTGGAAAACATCGA
CGTGATCGAAATCCATGAAGCATTCGCAGCAACTGCAGTGGCAGCTCTTGAAGAAATCAAAATCAGAACAGGATTTGATT
GGGAAAAGAACTTTGATGCCAAAAAGATCAATCCAAATGGTGGATCTATCGCCATTGGACACCCGTTTGGTGCGACTGGG
GTTCGCCTCCTACACAATGCGATCATGGACTTCCATGAAAACAAAGATGCAAAAAAGGTTCTCGTGACTGCTTGTGCACA
CGGTGGAATTGCAGGATCAATGATTGTAGAAAGACCATAA

Upstream 100 bases:

>100_bases
TGAAGCAGTAGGAACTAAACTACGTTCCATGATGAAGTGGCTCGCAAAGTAATTGTTTAGGTAACTTTAGTTAAAAGAAA
GAAAGGAAGAAGGATTATAT

Downstream 100 bases:

>100_bases
TTCGAATCTAAGATTCAATTACGTTCGTTCGAAAGATCGATTAAAAAGCCAAGGGTAACACCTTGGCTTTTTTTGTGGGG
AAATATTTTAAGAAATCGAA

Product: putative acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKVTQKIVLAAPARTPFAQIGKALAQYPGHHLGKLVGDEVMKRSGLKPTDIDGVIVGEGFANAPNSARVIANLMNLPLEI
PCLTVANNCVSGLEAVAEASRRIMLGEGEVFLVIGEESQTSMPFVVKNARLNKKTNSLDSLVKLLPNDLPEGVELRDTLE
DGLGDGETSYGMQVTAEILAQNYALPRETQDKVAYESFKRAFEATQEGRYKPYIMEVKDDEGNMLQADEAVLLREGLVKN
PTRMGRAMLMFDNPAMKFDAWKEKYGKDLKKSHGPTVSIFNASPRSDGAAGIIVASEAAAKRLGLKAEAVVTGFKMKGVD
PNLMGLGQAEATLGLLEEVGEKVENIDVIEIHEAFAATAVAALEEIKIRTGFDWEKNFDAKKINPNGGSIAIGHPFGATG
VRLLHNAIMDFHENKDAKKVLVTACAHGGIAGSMIVERP

Sequences:

>Translated_439_residues
MKVTQKIVLAAPARTPFAQIGKALAQYPGHHLGKLVGDEVMKRSGLKPTDIDGVIVGEGFANAPNSARVIANLMNLPLEI
PCLTVANNCVSGLEAVAEASRRIMLGEGEVFLVIGEESQTSMPFVVKNARLNKKTNSLDSLVKLLPNDLPEGVELRDTLE
DGLGDGETSYGMQVTAEILAQNYALPRETQDKVAYESFKRAFEATQEGRYKPYIMEVKDDEGNMLQADEAVLLREGLVKN
PTRMGRAMLMFDNPAMKFDAWKEKYGKDLKKSHGPTVSIFNASPRSDGAAGIIVASEAAAKRLGLKAEAVVTGFKMKGVD
PNLMGLGQAEATLGLLEEVGEKVENIDVIEIHEAFAATAVAALEEIKIRTGFDWEKNFDAKKINPNGGSIAIGHPFGATG
VRLLHNAIMDFHENKDAKKVLVTACAHGGIAGSMIVERP
>Mature_439_residues
MKVTQKIVLAAPARTPFAQIGKALAQYPGHHLGKLVGDEVMKRSGLKPTDIDGVIVGEGFANAPNSARVIANLMNLPLEI
PCLTVANNCVSGLEAVAEASRRIMLGEGEVFLVIGEESQTSMPFVVKNARLNKKTNSLDSLVKLLPNDLPEGVELRDTLE
DGLGDGETSYGMQVTAEILAQNYALPRETQDKVAYESFKRAFEATQEGRYKPYIMEVKDDEGNMLQADEAVLLREGLVKN
PTRMGRAMLMFDNPAMKFDAWKEKYGKDLKKSHGPTVSIFNASPRSDGAAGIIVASEAAAKRLGLKAEAVVTGFKMKGVD
PNLMGLGQAEATLGLLEEVGEKVENIDVIEIHEAFAATAVAALEEIKIRTGFDWEKNFDAKKINPNGGSIAIGHPFGATG
VRLLHNAIMDFHENKDAKKVLVTACAHGGIAGSMIVERP

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=446, Percent_Identity=28.0269058295964, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI148539872, Length=435, Percent_Identity=29.4252873563218, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI4557237, Length=439, Percent_Identity=25.5125284738041, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI4501853, Length=406, Percent_Identity=27.8325123152709, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4504327, Length=465, Percent_Identity=26.4516129032258, Blast_Score=102, Evalue=8e-22,
Organism=Escherichia coli, GI87082165, Length=441, Percent_Identity=31.7460317460317, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1787663, Length=435, Percent_Identity=29.8850574712644, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1788683, Length=436, Percent_Identity=29.1284403669725, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI48994986, Length=448, Percent_Identity=27.4553571428571, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1788554, Length=438, Percent_Identity=28.5388127853881, Blast_Score=131, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI133906874, Length=437, Percent_Identity=28.604118993135, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI25147385, Length=432, Percent_Identity=25.462962962963, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17535921, Length=444, Percent_Identity=25, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17551802, Length=466, Percent_Identity=28.1115879828326, Blast_Score=108, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6322031, Length=360, Percent_Identity=28.6111111111111, Blast_Score=135, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6325229, Length=443, Percent_Identity=25.5079006772009, Blast_Score=120, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24655093, Length=436, Percent_Identity=29.8165137614679, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI17648125, Length=445, Percent_Identity=29.6629213483146, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24640423, Length=440, Percent_Identity=27.2727272727273, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI17137578, Length=461, Percent_Identity=29.5010845986985, Blast_Score=128, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 47309; Mature: 47309

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTQKIVLAAPARTPFAQIGKALAQYPGHHLGKLVGDEVMKRSGLKPTDIDGVIVGEGF
CCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
ANAPNSARVIANLMNLPLEIPCLTVANNCVSGLEAVAEASRRIMLGEGEVFLVIGEESQT
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCEEEEECCCCCC
SMPFVVKNARLNKKTNSLDSLVKLLPNDLPEGVELRDTLEDGLGDGETSYGMQVTAEILA
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHH
QNYALPRETQDKVAYESFKRAFEATQEGRYKPYIMEVKDDEGNMLQADEAVLLREGLVKN
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEECCHHHHHHHCCCCC
PTRMGRAMLMFDNPAMKFDAWKEKYGKDLKKSHGPTVSIFNASPRSDGAAGIIVASEAAA
HHHHCCEEEEECCCCCCHHHHHHHHCHHHHHCCCCEEEEEECCCCCCCCEEEEEECHHHH
KRLGLKAEAVVTGFKMKGVDPNLMGLGQAEATLGLLEEVGEKVENIDVIEIHEAFAATAV
HHHCCCHHHEEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
AALEEIKIRTGFDWEKNFDAKKINPNGGSIAIGHPFGATGVRLLHNAIMDFHENKDAKKV
HHHHHHHHCCCCCCCCCCCCCEECCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHH
LVTACAHGGIAGSMIVERP
HHHHHHCCCCCCCEEEECC
>Mature Secondary Structure
MKVTQKIVLAAPARTPFAQIGKALAQYPGHHLGKLVGDEVMKRSGLKPTDIDGVIVGEGF
CCCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
ANAPNSARVIANLMNLPLEIPCLTVANNCVSGLEAVAEASRRIMLGEGEVFLVIGEESQT
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCEEEEECCCCCC
SMPFVVKNARLNKKTNSLDSLVKLLPNDLPEGVELRDTLEDGLGDGETSYGMQVTAEILA
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHH
QNYALPRETQDKVAYESFKRAFEATQEGRYKPYIMEVKDDEGNMLQADEAVLLREGLVKN
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEECCHHHHHHHCCCCC
PTRMGRAMLMFDNPAMKFDAWKEKYGKDLKKSHGPTVSIFNASPRSDGAAGIIVASEAAA
HHHHCCEEEEECCCCCCHHHHHHHHCHHHHHCCCCEEEEEECCCCCCCCEEEEEECHHHH
KRLGLKAEAVVTGFKMKGVDPNLMGLGQAEATLGLLEEVGEKVENIDVIEIHEAFAATAV
HHHCCCHHHEEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
AALEEIKIRTGFDWEKNFDAKKINPNGGSIAIGHPFGATGVRLLHNAIMDFHENKDAKKV
HHHHHHHHCCCCCCCCCCCCCEECCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHH
LVTACAHGGIAGSMIVERP
HHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]