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The map label for this gene is ytzG [H]

Identifier: 18311246

GI number: 18311246

Start: 2601132

End: 2601851

Strand: Reverse

Name: ytzG [H]

Synonym: CPE2264

Alternate gene names: 18311246

Gene position: 2601851-2601132 (Counterclockwise)

Preceding gene: 18311247

Following gene: 18311245

Centisome position: 85.83

GC content: 29.58

Gene sequence:

>720_bases
GTGGAAAGATTGGATAAGGTTTTATCAAACTTAGGATACGGAACGAGAAAAGAAATAAAGCAAGTTGTTAAAAAAGGATT
TGTAACAGTTAATGATGAGAAGGTTAAAGATAGTGGACTTCAAATAGATCCTGAGAAAGATACAATAGTTATAAATGGGG
AAAAAGTAGAGTATAAAAAGTACATATATTTAATGATGAATAAGCCAGCTGGAGTAATTTCAGCAACTTATGATAAGAGA
GATGAAACTGTTATCGACTTATTATATATGGAAGATCAAGTTTTTGAACCATTTCCAGTAGGAAGACTTGATAAAGATAC
AGTGGGATTATTATTATTAACTAATGATGGGGATTTAAATCATAAATTAATTTCACCTAAATACCATGTTGATAAGGTTT
ATTATGCAGAAATAAATGCTCCTATAACAGAAAAAGATATTAAAGCTTTCGAAAATGGTGTAACTTTAGATGATGGATAT
AAATGTATGAGTGCAAAGCTTAAGGTTATAGAAAATTTAGAGGATTCATCTAGAGTAGAAGTTACAGTTCAAGAGGGGAA
ATATCATCAAGTAAAAAGAATGTTTGAAGCTAGAGGGAAAAAGGTTGTTTATTTAAAAAGAACTTCTTTTGGAAATCTTC
CACTTGATGAAAGTTTAGAAGAAGGCGAATATAAAGAGCTTTCAGAAAGTGAATTAGCGGTACTTTACAAAAATTTTTAA

Upstream 100 bases:

>100_bases
TTTAATTATTTAAGAAAGATAATAAGTTGTAATTAGTTAAACTTTGCTTTATACTAACCACAGATATAAATGTGATTTTT
TACGAAGGAGTGACTATTTA

Downstream 100 bases:

>100_bases
AAATTTAAATAGTAATGATTGTAAAGGGAAAATTCATGTAGGAAAACTTGCAAAAAAAATATTAGATTACTTGCAATTTA
AGTAAACATCTAATATAATA

Product: 16S pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MERLDKVLSNLGYGTRKEIKQVVKKGFVTVNDEKVKDSGLQIDPEKDTIVINGEKVEYKKYIYLMMNKPAGVISATYDKR
DETVIDLLYMEDQVFEPFPVGRLDKDTVGLLLLTNDGDLNHKLISPKYHVDKVYYAEINAPITEKDIKAFENGVTLDDGY
KCMSAKLKVIENLEDSSRVEVTVQEGKYHQVKRMFEARGKKVVYLKRTSFGNLPLDESLEEGEYKELSESELAVLYKNF

Sequences:

>Translated_239_residues
MERLDKVLSNLGYGTRKEIKQVVKKGFVTVNDEKVKDSGLQIDPEKDTIVINGEKVEYKKYIYLMMNKPAGVISATYDKR
DETVIDLLYMEDQVFEPFPVGRLDKDTVGLLLLTNDGDLNHKLISPKYHVDKVYYAEINAPITEKDIKAFENGVTLDDGY
KCMSAKLKVIENLEDSSRVEVTVQEGKYHQVKRMFEARGKKVVYLKRTSFGNLPLDESLEEGEYKELSESELAVLYKNF
>Mature_239_residues
MERLDKVLSNLGYGTRKEIKQVVKKGFVTVNDEKVKDSGLQIDPEKDTIVINGEKVEYKKYIYLMMNKPAGVISATYDKR
DETVIDLLYMEDQVFEPFPVGRLDKDTVGLLLLTNDGDLNHKLISPKYHVDKVYYAEINAPITEKDIKAFENGVTLDDGY
KCMSAKLKVIENLEDSSRVEVTVQEGKYHQVKRMFEARGKKVVYLKRTSFGNLPLDESLEEGEYKELSESELAVLYKNF

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=233, Percent_Identity=34.7639484978541, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1790453, Length=238, Percent_Identity=31.0924369747899, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1787524, Length=234, Percent_Identity=30.7692307692308, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87081838, Length=191, Percent_Identity=28.2722513089005, Blast_Score=80, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27410; Mature: 27410

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLDKVLSNLGYGTRKEIKQVVKKGFVTVNDEKVKDSGLQIDPEKDTIVINGEKVEYKK
CHHHHHHHHHCCCCCHHHHHHHHHHCCEEECCCCCCCCCCEECCCCCEEEECCCEEEEEE
YIYLMMNKPAGVISATYDKRDETVIDLLYMEDQVFEPFPVGRLDKDTVGLLLLTNDGDLN
EEEEEECCCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCC
HKLISPKYHVDKVYYAEINAPITEKDIKAFENGVTLDDGYKCMSAKLKVIENLEDSSRVE
CEEECCCCCCCEEEEEEECCCCCHHHHHHHHCCCEECCCHHHHHHHHHHHHCCCCCCEEE
VTVQEGKYHQVKRMFEARGKKVVYLKRTSFGNLPLDESLEEGEYKELSESELAVLYKNF
EEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHCCCCCEEEEEECC
>Mature Secondary Structure
MERLDKVLSNLGYGTRKEIKQVVKKGFVTVNDEKVKDSGLQIDPEKDTIVINGEKVEYKK
CHHHHHHHHHCCCCCHHHHHHHHHHCCEEECCCCCCCCCCEECCCCCEEEECCCEEEEEE
YIYLMMNKPAGVISATYDKRDETVIDLLYMEDQVFEPFPVGRLDKDTVGLLLLTNDGDLN
EEEEEECCCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCC
HKLISPKYHVDKVYYAEINAPITEKDIKAFENGVTLDDGYKCMSAKLKVIENLEDSSRVE
CEEECCCCCCCEEEEEEECCCCCHHHHHHHHCCCEECCCHHHHHHHHHHHHCCCCCCEEE
VTVQEGKYHQVKRMFEARGKKVVYLKRTSFGNLPLDESLEEGEYKELSESELAVLYKNF
EEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]