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The map label for this gene is rph [H]

Identifier: 18311236

GI number: 18311236

Start: 2587100

End: 2587846

Strand: Reverse

Name: rph [H]

Synonym: CPE2254

Alternate gene names: 18311236

Gene position: 2587846-2587100 (Counterclockwise)

Preceding gene: 18311238

Following gene: 18311235

Centisome position: 85.37

GC content: 33.6

Gene sequence:

>747_bases
ATGAGAAGTAGTGGAAGAAAAAAAGAACAGATAAGACCAGTTAAAATAACAAGAAACTTTACTAAATATGCAGAAGGCTC
TGTATTAATAGAAGTTGGGGATACTAAAGTATTATGTACTGCTTCAATAGAAGAGAAAGTTCCACCTTTTTTAAAGGGTT
CTGGAGAAGGATGGATAACTGCTGAATATAACATGCTTCCAAGAAGTACTCAAAGTAGAAAACAAAGAGATATAAATAAA
CTTAAAATAGATGGAAGAACAATGGAAATACAAAGACTTATAGGAAGAGCTCTTAGATCAGCTGTAGATATGAAAGCTTT
AGGAGAAAAAACTATTTGGATAGACTGTGACGTTCTTCAAGCTGATGGGGGAACTAGAACGACTTCTATTACAGGTTCAT
TTGTGGCATTAGTTGATGCTGTAAATAAACTTCATCAAAAGAAACCTTTCAATGTTTATCCTATAAGACATTTTGTCAGC
GCAGTAAGTGTTGGAATAGTAGGGGAAGAGAAAATATTAGACTTATGCTATGAAGAAGACCATGTAGCTAAAGTAGATAT
GAATGTTGTAATGACAGAAGAGGGAGAATTTATAGAAATTCAAGGAACTGGAGAGGCTGGACCTTTTTCAAGAAAAGAAT
TAGATGAATTATTAAATCTTGCTGAAAAAGGAGCAAAACAAATGATACAAGCACAAAAAGATGCTTTAAAGACAGATTCT
TTATGGATCGGAACAGGGAGAGAGTAA

Upstream 100 bases:

>100_bases
TAATTTTTATATATAATCCATGTTGATAAAGGATAAAATAACCAATATACTATTCTTATAATTAGTGTTTTGAAAATAAA
TTAAGGTAAGGAGATATTGA

Downstream 100 bases:

>100_bases
AATGAAAAAATTTATACTAGCAAGTAATAATGCACATAAGGTAAAAGAAATAAAAGAAATATTAAAAGATTTTAATTTAA
ACATATTATCTTTAAATGAA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MRSSGRKKEQIRPVKITRNFTKYAEGSVLIEVGDTKVLCTASIEEKVPPFLKGSGEGWITAEYNMLPRSTQSRKQRDINK
LKIDGRTMEIQRLIGRALRSAVDMKALGEKTIWIDCDVLQADGGTRTTSITGSFVALVDAVNKLHQKKPFNVYPIRHFVS
AVSVGIVGEEKILDLCYEEDHVAKVDMNVVMTEEGEFIEIQGTGEAGPFSRKELDELLNLAEKGAKQMIQAQKDALKTDS
LWIGTGRE

Sequences:

>Translated_248_residues
MRSSGRKKEQIRPVKITRNFTKYAEGSVLIEVGDTKVLCTASIEEKVPPFLKGSGEGWITAEYNMLPRSTQSRKQRDINK
LKIDGRTMEIQRLIGRALRSAVDMKALGEKTIWIDCDVLQADGGTRTTSITGSFVALVDAVNKLHQKKPFNVYPIRHFVS
AVSVGIVGEEKILDLCYEEDHVAKVDMNVVMTEEGEFIEIQGTGEAGPFSRKELDELLNLAEKGAKQMIQAQKDALKTDS
LWIGTGRE
>Mature_248_residues
MRSSGRKKEQIRPVKITRNFTKYAEGSVLIEVGDTKVLCTASIEEKVPPFLKGSGEGWITAEYNMLPRSTQSRKQRDINK
LKIDGRTMEIQRLIGRALRSAVDMKALGEKTIWIDCDVLQADGGTRTTSITGSFVALVDAVNKLHQKKPFNVYPIRHFVS
AVSVGIVGEEKILDLCYEEDHVAKVDMNVVMTEEGEFIEIQGTGEAGPFSRKELDELLNLAEKGAKQMIQAQKDALKTDS
LWIGTGRE

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=65.8767772511848, Blast_Score=285, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI71981632, Length=180, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 27629; Mature: 27629

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSGRKKEQIRPVKITRNFTKYAEGSVLIEVGDTKVLCTASIEEKVPPFLKGSGEGWIT
CCCCCCCHHCCCCEEEECCHHHHCCCEEEEEECCCEEEEEECHHHHCCCCCCCCCCEEEE
AEYNMLPRSTQSRKQRDINKLKIDGRTMEIQRLIGRALRSAVDMKALGEKTIWIDCDVLQ
EECCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTTSITGSFVALVDAVNKLHQKKPFNVYPIRHFVSAVSVGIVGEEKILDLCYEED
CCCCCEEEEECHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHCCCC
HVAKVDMNVVMTEEGEFIEIQGTGEAGPFSRKELDELLNLAEKGAKQMIQAQKDALKTDS
CEEEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LWIGTGRE
EEEECCCC
>Mature Secondary Structure
MRSSGRKKEQIRPVKITRNFTKYAEGSVLIEVGDTKVLCTASIEEKVPPFLKGSGEGWIT
CCCCCCCHHCCCCEEEECCHHHHCCCEEEEEECCCEEEEEECHHHHCCCCCCCCCCEEEE
AEYNMLPRSTQSRKQRDINKLKIDGRTMEIQRLIGRALRSAVDMKALGEKTIWIDCDVLQ
EECCCCCCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
ADGGTRTTSITGSFVALVDAVNKLHQKKPFNVYPIRHFVSAVSVGIVGEEKILDLCYEED
CCCCCEEEEECHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHCCCC
HVAKVDMNVVMTEEGEFIEIQGTGEAGPFSRKELDELLNLAEKGAKQMIQAQKDALKTDS
CEEEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LWIGTGRE
EEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA